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A) HE pathology staining, <t>MAP2</t> immunohistochemistry, and silver staining of brain tissue from various groups of mice. The right side of panel A shows a schematic of the observed brain damage areas (Image credit: Allen Institute for Brain Science), with black dots indicating the damaged areas, primarily in the hippocampal region. B) Statistical count of infiltrated immune cells and microvessels affected by thromboembolic events in each group of mice. * p < 0.05, ** p < 0.01.

Map2, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/map2/anti+map2/pmc13196981-101-8-11
Average 86 stars, based on 1 article reviews

map2 - by Bioz Stars, 2026-10

86/100 stars

Images

1) Product Images from "Inhibition of neutrophil infiltration and NETs formation ameliorates neuropsychiatric and renal dysfunction in MRL/lpr mice with lupus"

Article Title: Inhibition of neutrophil infiltration and NETs formation ameliorates neuropsychiatric and renal dysfunction in MRL/lpr mice with lupus

Journal: PLOS One

doi: 10.1371/journal.pone.0348011

A) HE pathology staining, MAP2 immunohistochemistry, and silver staining of brain tissue from various groups of mice. The right side of panel A shows a schematic of the observed brain damage areas (Image credit: Allen Institute for Brain Science), with black dots indicating the damaged areas, primarily in the hippocampal region. B) Statistical count of infiltrated immune cells and microvessels affected by thromboembolic events in each group of mice. * p < 0.05, ** p < 0.01.

Figure Legend Snippet: A) HE pathology staining, MAP2 immunohistochemistry, and silver staining of brain tissue from various groups of mice. The right side of panel A shows a schematic of the observed brain damage areas (Image credit: Allen Institute for Brain Science), with black dots indicating the damaged areas, primarily in the hippocampal region. B) Statistical count of infiltrated immune cells and microvessels affected by thromboembolic events in each group of mice. * p < 0.05, ** p < 0.01.

Techniques Used: Staining, Immunohistochemistry, Silver Staining

A) Upper half: Multiplex immunofluorescence staining of CD11b, CD16, and MAP2 in brain sections from various groups of mice, with localized positive areas enlarged; white arrowheads indicate CD11b/CD16 double-positive cells. Lower half: Multiplex immunofluorescence staining of NE and MPO, with localized positive areas enlarged. The brain region schematic used as a reference is derived from the Allen Brain Atlas. B) and C) Statistical count of CD11b positive and CD11b/CD16 double-positive cells across different groups. D) and E) Semi-quantitative analysis of fluorescence intensity for NE and MPO in different groups. Statistical significance is indicated as follows: * p < 0.05, ** p < 0.01 compared to the MRL/MpJ group; # p < 0.05, ## p < 0.01 compared to the MRL/lpr+Avacopan group.

Figure Legend Snippet: A) Upper half: Multiplex immunofluorescence staining of CD11b, CD16, and MAP2 in brain sections from various groups of mice, with localized positive areas enlarged; white arrowheads indicate CD11b/CD16 double-positive cells. Lower half: Multiplex immunofluorescence staining of NE and MPO, with localized positive areas enlarged. The brain region schematic used as a reference is derived from the Allen Brain Atlas. B) and C) Statistical count of CD11b positive and CD11b/CD16 double-positive cells across different groups. D) and E) Semi-quantitative analysis of fluorescence intensity for NE and MPO in different groups. Statistical significance is indicated as follows: * p < 0.05, ** p < 0.01 compared to the MRL/MpJ group; # p < 0.05, ## p < 0.01 compared to the MRL/lpr+Avacopan group.

Techniques Used: Multiplex Assay, Immunofluorescence, Staining, Derivative Assay, Fluorescence

In Vitro:

Article Title: DCPIB Attenuates Ischemia-Reperfusion Injury by Regulating Microglial M1/M2 Polarization and Oxidative Stress.
Article Snippet: The inflammatory response plays an indispensable role in ischemia–reperfusion injury, the most significant of which is the inflammatory response caused by microglial polarization.. Anti‐inflammatory therapy is also an important remedial measure after failed vascular reconstruction.. Maintaining the internal homeostasis of the brain is a crucial measure for suppressing the inflammatory response.

Incubation:

Article Title: Buyang Huanwu decoction alleviates oxidative injury of cerebral ischemia-reperfusion through PKCε/Nrf2 signaling pathway.
Article Snippet: Ethnopharmacological relevance: Ischemic stroke is a significant risk factor for human health, and Buyang Huanwu Decoction is a classical and famous Chinese formula for treating it, but without clear pharmacological

Article Title: Engrailed-1 potentiates mitochondrial transplant neuroprotection in spinal cord ischemia-reperfusion injury.
Article Snippet: .. The tissue was then incubated with anti-Engrailed-1 (1:100, sc-398534 X, Santa Cruz, USA), anti-MAP2 (GB11128-2, Servicebio, China), and anti-GFAP (GB11096, Servicebio, China) antibodies. .. Subsequent to staining with secondary antibodies (iF647, G1232; iF488, G1231; iF546, G1251, Servicebio, China) and DAPI (G1012, Servicebio, China), the stained sections were imaged using a slide scanner (Pannoramic MIDI, 3DHISTECH, Magyar).

Immunostaining:

Article Title: Synthetic Disaggregators Enhance Central-Peripheral Amyloid-β Clearance in Alzheimer's Disease.
Article Snippet: Pathogenic amyloid-β (Aβ) accumulation defines Alzheimer’s disease (AD), directly inflicting neuronal damage and driving chronic neuroinflammation.. While both central microglia and peripheral macrophages are critical for Aβ clearance, their functional impairment in AD inexorably leads to escalating Aβ burden and disease progression.. We here report an in situ engineered synthetic Aβ disaggregator (SAD) delivered to macrophages via neuroprotective DHA-based lipid nanoparticles (DLNPs).

Image Search Results

Syb2‐containing neuronal EV uptake in the somatodendritic compartment depends on Dynamin and occurs independently of macropinocytosis. (A) Experimental design. (B) Representative confocal images of neuronal cell bodies labeled with Dex (yellow), immunofluorescently labeled with anti‐MAP2 (magenta) and anti‐GFP (cyan). Z‐stacks of images are shown, presented as the maximum intensity projection of the volumetric data. White scale bars = 20 μm. (C, D) Number of Dex and GFP (EV‐delivered Syb2‐pHluorin) clusters in negative control, DMSO‐treated, and EIPA‐treated groups. (C) Kruskal–Wallis non‐parametric test, Kruskal–Wallis statistic = 50.83, p < 0.0001. (D) Kruskal – Wallis non‐parametric test, Kruskal–Wallis statistic = 27.15, p < 0.0001. (E) Experimental design. (F) Representative confocal images of neuronal cell bodies labeled with Tf (yellow), immunofluorescently labeled with anti‐MAP2 (magenta) and anti‐GFP (cyan). Z‐stacks of images are shown, presented as the maximum intensity projection of the volumetric data. White scale bars = 20 μm. (G, H) Number of Tf and GFP (EV‐delivered Syb2‐pHluorin) clusters in negative control, DMSO‐treated, and EIPA‐treated groups. (G) Kruskal – Wallis non‐parametric test, Kruskal–Wallis statistic = 49.51, p < 0.0001. (H) Kruskal – Wallis non‐parametric test, Kruskal–Wallis statistic = 29.31, p < 0.0001. For C, D and G, H: data are shown as box and whisker plots with each dot representing one individual neuron; results from Dunn's multiple comparisons test are displayed on the figure; data from 3 independent cultures.

Journal: Journal of Neurochemistry

Article Title: Synaptobrevin‐2 Containing Extracellular Vesicles Are Rapidly Incorporated Into Mammalian Neurons via a Dynamin‐Dependent Pathway

doi: 10.1111/jnc.70551

Figure Lengend Snippet: Syb2‐containing neuronal EV uptake in the somatodendritic compartment depends on Dynamin and occurs independently of macropinocytosis. (A) Experimental design. (B) Representative confocal images of neuronal cell bodies labeled with Dex (yellow), immunofluorescently labeled with anti‐MAP2 (magenta) and anti‐GFP (cyan). Z‐stacks of images are shown, presented as the maximum intensity projection of the volumetric data. White scale bars = 20 μm. (C, D) Number of Dex and GFP (EV‐delivered Syb2‐pHluorin) clusters in negative control, DMSO‐treated, and EIPA‐treated groups. (C) Kruskal–Wallis non‐parametric test, Kruskal–Wallis statistic = 50.83, p < 0.0001. (D) Kruskal – Wallis non‐parametric test, Kruskal–Wallis statistic = 27.15, p < 0.0001. (E) Experimental design. (F) Representative confocal images of neuronal cell bodies labeled with Tf (yellow), immunofluorescently labeled with anti‐MAP2 (magenta) and anti‐GFP (cyan). Z‐stacks of images are shown, presented as the maximum intensity projection of the volumetric data. White scale bars = 20 μm. (G, H) Number of Tf and GFP (EV‐delivered Syb2‐pHluorin) clusters in negative control, DMSO‐treated, and EIPA‐treated groups. (G) Kruskal – Wallis non‐parametric test, Kruskal–Wallis statistic = 49.51, p < 0.0001. (H) Kruskal – Wallis non‐parametric test, Kruskal–Wallis statistic = 29.31, p < 0.0001. For C, D and G, H: data are shown as box and whisker plots with each dot representing one individual neuron; results from Dunn's multiple comparisons test are displayed on the figure; data from 3 independent cultures.

Article Snippet: After blocking with 3% BSA, primary antibodies against GFP (1:150) (Invitrogen, Cat. No. A11122, RRID:AB_221569), Syn1 (1:500) (Synaptic Systems, Cat. No. 106011, RRID:AB_10805139), Syp1 (1:500) (Synaptic Systems, Cat. No. 101006, RRID: AB_2622239), and/or MAP2 (1:1000) (Synaptic Systems, Cat. No. 188004, RRID:AB_2138181) were diluted in blocking buffer and incubated overnight at 4°C in a humid chamber.

Techniques: Labeling, Negative Control, Whisker Assay

Syb2‐containing neuronal EV uptake in the axonal compartment is Dynamin‐dependent and occurs independently of macropinocytosis. (A) Experimental design. (B) Representative confocal images of synapses labeled with Dex (yellow), immunofluorescently labeled with anti‐Syp1 (magenta) and anti‐GFP (cyan). Z‐stacks of images are shown, presented as the maximum intensity projection of the volumetric data. White scale bars = 5 μm. (C, D) Number of Dex and GFP (EV‐delivered Syb2‐pHluorin) clusters in negative control, DMSO‐treated, and EIPA‐treated groups. (C) Kruskal‐Wallis non‐parametric test, Kruskal–Wallis statistic = 48.26, p < 0.0001. (D) Kruskal–Wallis non‐parametric test, Kruskal–Wallis statistic = 46.09, p < 0.0001. (E) Experimental design. (F) Representative confocal images of neuronal cell bodies labeled with Tf (yellow), immunofluorescently labeled with anti‐MAP2 (magenta) and anti‐GFP (cyan). Z‐stacks of images are shown, presented as the maximum intensity projection of the volumetric data. White scale bars = 5 μm. (G, H) Number of Tf and GFP (EV‐delivered Syb2‐pHluorin) clusters in negative control, DMSO‐treated, and EIPA‐treated groups. (G) Kruskal–Wallis non‐parametric test, Kruskal–Wallis statistic = 35.22, p < 0.0001. (D) Kruskal‐Wallis non‐parametric test, Kruskal–Wallis statistic = 30.51, p < 0.0001. For C, D and G, H: data are shown as box and whisker plots and each dot is the average of all presynaptic boutons quantified in one image; results from Dunn's multiple comparisons test are displayed on the figure; data from 3 independent cultures.

Journal: Journal of Neurochemistry

Article Title: Synaptobrevin‐2 Containing Extracellular Vesicles Are Rapidly Incorporated Into Mammalian Neurons via a Dynamin‐Dependent Pathway

doi: 10.1111/jnc.70551

Figure Lengend Snippet: Syb2‐containing neuronal EV uptake in the axonal compartment is Dynamin‐dependent and occurs independently of macropinocytosis. (A) Experimental design. (B) Representative confocal images of synapses labeled with Dex (yellow), immunofluorescently labeled with anti‐Syp1 (magenta) and anti‐GFP (cyan). Z‐stacks of images are shown, presented as the maximum intensity projection of the volumetric data. White scale bars = 5 μm. (C, D) Number of Dex and GFP (EV‐delivered Syb2‐pHluorin) clusters in negative control, DMSO‐treated, and EIPA‐treated groups. (C) Kruskal‐Wallis non‐parametric test, Kruskal–Wallis statistic = 48.26, p < 0.0001. (D) Kruskal–Wallis non‐parametric test, Kruskal–Wallis statistic = 46.09, p < 0.0001. (E) Experimental design. (F) Representative confocal images of neuronal cell bodies labeled with Tf (yellow), immunofluorescently labeled with anti‐MAP2 (magenta) and anti‐GFP (cyan). Z‐stacks of images are shown, presented as the maximum intensity projection of the volumetric data. White scale bars = 5 μm. (G, H) Number of Tf and GFP (EV‐delivered Syb2‐pHluorin) clusters in negative control, DMSO‐treated, and EIPA‐treated groups. (G) Kruskal–Wallis non‐parametric test, Kruskal–Wallis statistic = 35.22, p < 0.0001. (D) Kruskal‐Wallis non‐parametric test, Kruskal–Wallis statistic = 30.51, p < 0.0001. For C, D and G, H: data are shown as box and whisker plots and each dot is the average of all presynaptic boutons quantified in one image; results from Dunn's multiple comparisons test are displayed on the figure; data from 3 independent cultures.

Techniques: Labeling, Negative Control, Whisker Assay

Journal: Cell reports

Article Title: Pleiotrophin regulates presynaptic assembly and function through heparan sulfate-dependent binding to neurexin1

doi: 10.1016/j.celrep.2026.117827

Figure Lengend Snippet:

Article Snippet: Chicken anti-MAP2 , Synaptic Systems , Cat# 188006; RRID:AB_2619881.

Techniques: Virus, Recombinant, Magnetic Beads, Mass Spectrometry, shRNA, Sequencing, Software

Waveform rescue and impairment of AIS in C9-MSNs (A) Example AP waveforms for Con-1 (blue) and C9-3 MSNs (black) before and after the addition of BK channel activator NS11021 (10 μM, lighter color shade). Scale bars, 20 mV, 10 ms. (B and C) Mean ± SEM AP amplitude, AHP, half-width, threshold, and rheobase of C9-3 MSNs in the presence and absence of NS11021, along with Con-1 MSNs, were examined in parallel. Statistics, ∗ p < 0.05 and ∗∗∗ p < 0.001, from paired t test or t test . Data: con-1: n = 8, N = 3. C9-3: n = 10, N = 4. (D) NS11021 treatment had no effect on AP output in C9-3 MSNs evoked from current stimulation. Con-1 patched in parallel, shown for reference. Data: Con-1: n = 8, N = 3. C9-3: n = 10, N = 4. Statistics, two-way repeated measures ANOVA followed by Tukey’s multiple comparisons test. (E) Images of Con-1 and C9-3 stained with AIS marker ankyrin-G, neuronal marker MAP2, and DAPI. Scale bars, 50 μm. (F) Mean ± SEM AIS length and distance of start of AIS from the cell soma. Data: Con-1: n = 16, N = 4. C9-3: n = 36, N = 4. ∗∗∗ p < 0.001, t test.

Journal: Cell Reports

Article Title: Striatal neuron dysfunction in C9ORF72-FTD/ALS is driven by AIS and potassium channel dysregulation

doi: 10.1016/j.celrep.2026.117672

Figure Lengend Snippet: Waveform rescue and impairment of AIS in C9-MSNs (A) Example AP waveforms for Con-1 (blue) and C9-3 MSNs (black) before and after the addition of BK channel activator NS11021 (10 μM, lighter color shade). Scale bars, 20 mV, 10 ms. (B and C) Mean ± SEM AP amplitude, AHP, half-width, threshold, and rheobase of C9-3 MSNs in the presence and absence of NS11021, along with Con-1 MSNs, were examined in parallel. Statistics, ∗ p < 0.05 and ∗∗∗ p < 0.001, from paired t test or t test . Data: con-1: n = 8, N = 3. C9-3: n = 10, N = 4. (D) NS11021 treatment had no effect on AP output in C9-3 MSNs evoked from current stimulation. Con-1 patched in parallel, shown for reference. Data: Con-1: n = 8, N = 3. C9-3: n = 10, N = 4. Statistics, two-way repeated measures ANOVA followed by Tukey’s multiple comparisons test. (E) Images of Con-1 and C9-3 stained with AIS marker ankyrin-G, neuronal marker MAP2, and DAPI. Scale bars, 50 μm. (F) Mean ± SEM AIS length and distance of start of AIS from the cell soma. Data: Con-1: n = 16, N = 4. C9-3: n = 36, N = 4. ∗∗∗ p < 0.001, t test.

Article Snippet: MAP2 anti-Rabbit (1:1000) , Synaptic Systems , Cat# 188 003; RRID AB_2281442.

Techniques: Staining, Marker