gene exp map2 mm00485231 m1 (Thermo Fisher)
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gene exp map2 mm00485231 m1
Gene Exp Map2 Mm00485231 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/map2/Gene+Exp%2E+Map2%2C+Mm00485231_m1/pmc13115098-100-29--1
Average 94 stars, based on 1 article reviews
Gene Exp Map2 Mm00485231 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/map2/Gene+Exp%2E+Map2%2C+Mm00485231_m1/pmc13115098-100-29--1
Average 94 stars, based on 1 article reviews
gene exp map2 mm00485231 m1 - by Bioz Stars,
2026-11
94/100 stars
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1) Product Images from "Astro-Versus Microglia-Enriched Transcriptomes from Aged Atxn2 -CAG100-Knockin Mice Suggest Underlying Pathology of RNA Processing at Ribosomes, and Possibly at U-Bodies"
Article Title: Astro-Versus Microglia-Enriched Transcriptomes from Aged Atxn2 -CAG100-Knockin Mice Suggest Underlying Pathology of RNA Processing at Ribosomes, and Possibly at U-Bodies
Journal: Cells
doi: 10.3390/cells15080699
Figure Legend Snippet: Purity validation of isolated microglia, astrocytes, and neurons. ( A ) Expression of specific markers in the gene sequencing data of 10-month-old mice. ( B ) Viable cell counts from 3-day-old wild-type mice ( n = 3). Data are mean ± SEM. ( C ) Immunofluorescence staining of microglia with the marker IBA1, astrocytes with GFAP, and neurons with MAP2 after 5 days in culture. DAPI was used for staining the nucleus. Scale bar: 5 µM.
Techniques Used: Biomarker Discovery, Isolation, Expressing, Sequencing, Immunofluorescence, Staining, Marker
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other:Article Title: Critical Roles of Translation Initiation and RNA Uridylation in Endogenous Retroviral Expression and Neural Differentiation in Pluripotent Stem Cells. Article Snippet: Oligonucleotides Sequences of primers, gRNA and the homologous regions for gene targeting used in this study This study see Table S3 Recombinant DNA PB-TRE-NAT1-CRB This study N/A PB-TRE-NAT1-3xFLAG-CRZ This study N/A pHL-H1-ccdB-EF1a-RiH (Li et al., 2015) Addgene #60601 pHL-H1-EF1a-RiH: human NAT1 gRNA This study N/A pHL-EF1a-SphcCas9-iC-A (Li et al., 2015) Addgene #60599 eSpCas9 (1.1) (Slaymaker et al., 2016) Addgene #71814 eSpCas9 (1.1): mouse NAT1 gRNA This study N/A PB-U6-CNCB This study N/A PB-U6-CNCB: human NAT1 gRNA This study N/A PB-U6-CNCB: human TUT7 gRNA This study N/A pCW-hyPBase This study N/A SB-CAG-NAT1-3xFLAG-CRZ This study N/A SB-U6-CNKH This study N/A SB-U6-CNKH: HERV-H shRNA This study N/A pCW-SB100X This study N/A pMXs-OCT3/4 Addgene #17217 pMXs-SOX2 Addgene #17218 pMXs-KLF4 Addgene #17219 pMXs-c-MYC Addgene #17220 pMXs CELL BIOLABS, INC. RTV-010 pMD2.G Addgene #12259, RRID:Addgene_12259 pGL4.13 Promega E6681 pGL4.13: human TUT7 50 UTR This study N/A pGL4.13: mouse TUT7 50 UTR This study N/A pGL4.13: human GAPDH 50 UTR This study N/A pGL4.74 Promega E6921 pGL4.13+RL This study N/A pGL4.13+RL: human TUT7 50 UTR This study N/A pGL4.13+RL: mouse TUT7 50 UTR This study N/A pGL4.13+RL: EMCV IRES This study N/A pBS-Puro-bGHpA This study N/A pBS-Neo-SV40pA This study N/A pBS-BSD-SV40pA This study N/A Human NAT1 targeting vector (Puro) This study N/A Human NAT1 targeting vector (Neo) This study N/A Mouse NAT1 targeting vector (Puro) This study N/A Mouse NAT1 targeting vector (BSD) This study N/A Software and Algorithms GeneSpring version 14.9.1 https://www.agilent.com/ Agilent Technologies DAVID Bioinformatics Resources 6.7 https://david.ncifcrf.gov/home.jsp (Huang et al., 2009) fastq-mcf. (in ea-utils) https://expressionanalysis.github.io/ ea-utils/ (Aronesty, 2013) (Continued on next page) Cell Reports 31, 107715, June 2, 2020 e6 Article Title: Effects of Tributyltin Chloride on Human Neuronal Differentiation and Mice Brain Development. Article Snippet: Probes were as follows: Gapdh: Mm99999915_ g1; Ppargc1a: Mm01208835_m1; Tubb3: Mm00727586_s1; Map2: Mm00485231_m1. Article Title: A pre-existing Toxoplasma gondii infection exacerbates the pathophysiological response and extent of brain damage after traumatic brain injury in mice Article Snippet: 18 , MAP2 , Mm00485231_m1 , 44 , CCL12 , Mm01617100_m1. Article Title: Astro-Versus Microglia-Enriched Transcriptomes from Aged Atxn2 -CAG100-Knockin Mice Suggest Underlying Pathology of RNA Processing at Ribosomes, and Possibly at U-Bodies Article Snippet: The following Taqman assays were used: Aif1 : Mm00520165_m1, Atxn2 : Mm01199894_m1, Cd31 : Mm01242576_m1, Cldn5 : Mm00727012_s1, Dlg4 : Mm00492193_m1 Gfap : Mm01253033_m1, Kcnj8 : Mm00434620_m1, Map2 : Mm00485231_m1, Olig2 : Mm01210556_m1, Tmem119 : Mm00525305_m1, and Tbp : Mm00446973_m1. TaqMan Assay:Article Title: Critical roles of translation initiation and RNA uridylation in endogenous retroviral expression and neural differentiation in pluripotent stem cells Article Snippet: Gfap (Mm01253033_m1) TaqMan Assay , Thermo Fisher Scientific , 4331182. .. Map2 ( Polymerase Chain Reaction:Article Title: Lysine deacetylation inhibition reverses TDP-43 mislocalization and in combination with arimoclomol ameliorates neuromuscular pathology Article Snippet: For quantitative real-time polymerase chain reactions (RT-PCR), we used the Taq Man Gene expression assay (Applied Biosystems, Waltham, MA. .. USA), on cDNA specimens in triplicate, using 1X Universal PCR master mix (Life Technologies, Carlsbad, CA, USA) and 1X mix containing specific receptor probes for mouse acetylcholine receptor ( AChR ) γ -subunit in muscle (Mm00437419_m1; Life Technologies, Carlsbad, CA, USA), microtubule associated protein 2 ( Map-2 ) ( Isolation:Article Title: Thy1-YFP: an effective tool for single cell tracing from neuronal progenitors to mature functionally active neurons Article Snippet: .. Total RNA was isolated by using commercial RNeasy® Mini Kit (Qiagen, 74104) and converted to the cDNA using high-capacity RNA-to-cDNA Kit (Applied Biosystems, 4368814). qPCR was performed on cells in the same time points for five assays: Nestin (Mm00450205_m1), Map2 ( Article Title: Thy1-YFP: an effective tool for single cell tracing from neuronal progenitors to mature functionally active neurons. Article Snippet: .. Full length blots are provided in the Supplementary information - Original data. qPCR Total RNA was isolated by using commercial RNeasy® Mini Kit (Qiagen, 74104) and converted to the cDNA using high-capacity RNA-to-cDNA Kit (Applied Biosystems, 4368814). qPCR was performed on cells in the same time points for five assays: Nestin (Mm00450205_m1), Map2 ( Real-time Polymerase Chain Reaction:Article Title: Thy1-YFP: an effective tool for single cell tracing from neuronal progenitors to mature functionally active neurons Article Snippet: .. Total RNA was isolated by using commercial RNeasy® Mini Kit (Qiagen, 74104) and converted to the cDNA using high-capacity RNA-to-cDNA Kit (Applied Biosystems, 4368814). qPCR was performed on cells in the same time points for five assays: Nestin (Mm00450205_m1), Map2 ( Article Title: Thy1-YFP: an effective tool for single cell tracing from neuronal progenitors to mature functionally active neurons. Article Snippet: .. Full length blots are provided in the Supplementary information - Original data. qPCR Total RNA was isolated by using commercial RNeasy® Mini Kit (Qiagen, 74104) and converted to the cDNA using high-capacity RNA-to-cDNA Kit (Applied Biosystems, 4368814). qPCR was performed on cells in the same time points for five assays: Nestin (Mm00450205_m1), Map2 ( |
