macsplex buffer (Miltenyi Biotec)
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Miltenyi Biotec
macsplex buffer
Macsplex Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macsplex+buffer/mac+buf/pm36577715-66-9-24
Average 90 stars, based on 1 article reviews
Macsplex Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macsplex+buffer/mac+buf/pm36577715-66-9-24
Average 90 stars, based on 1 article reviews
macsplex buffer - by Bioz Stars,
2026-10
90/100 stars
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Magnetic Cell Separation:Article Title: Derivation and transcriptional reprogramming of border-forming wound repair astrocytes after spinal cord injury or stroke in mice Article Snippet: Nuclei were pelleted by centrifugation (model 5415R, Eppendorf; 500 r.p.m. for 5 min at 4 °C) and then resuspended in nuclei wash and resuspension buffer (1× PBS, 1% BSA, 0.2 U ul −1 RNase inhibitor) before being washed once more in nuclei wash and resuspension buffer and then filtered using a 5 ml polystyrene round-bottom tube with 35 μm cell-strainer cap and concentrated to a nuclei concentration of 1,000 nuclei per μl (1 × 10 6 nuclei per ml), resuspended and incubated with Sox9 rabbit monoclonal antibody (ThermoFisher, 72016) for 30 min and then centrifuged at 700× g for 10 min. Pellets were resuspended in 80 μl of MACS buffer composed of 1× PBS (tissue culture grade; Ca 2+ , Mg 2+ free), 0.5% nuclease-free BSA, and 2 mM EDTA, and then incubated with anti-Rabbit IgG Microbeads (Miltenyi, 130-048-602) for 20 min at 4 °C. .. After washing with 1 ml of Article Title: Anti-IL-33 antibodies and uses thereof Article Snippet: K2 EDTA whole blood was diluted 1:1 in RPMI 1640, carefully layered over Ficoll-Paque (GE Healthcare, #17-1440-03) and centrifuged to separate PBMC. .. The interphase layer containing the PBMC was aspirated, transferred to a new tube, and washed twice with Article Title: SGLT2 inhibitors attenuate endothelial to mesenchymal transition and cardiac fibroblast activation. Article Snippet: One quarter of the of the cell suspension volume human anti-fibroblast Microbeads (Miltenyi Biotec) were added for 30 min at RT, light protected. .. Afterwards, two washing steps with 1 mL Article Title: Synergy and antagonism in the integration of BCR and CD40 signals that control B-cell proliferation Article Snippet: .. Homogenized splenocytes were incubated with anti-CD43 magnetic beads for 15 min at 4-8°C, washed with Article Title: IL-2 and TCR stimulation induce expression and secretion of IL-32β by human T cells Article Snippet: To exclude dead cells, after washing with PBS (Sigma, Cat#D1408), the cells were stained with the Zombie NIRTM Fixable Viability Kit (BioLegend, Cat#423105) or the Zombie AquaTM Fixable Viability Kit (BioLegend, Cat#423102) according to the manufacturer’s recommendations. .. To analyze CD25 expression, cells were first washed with Article Title: SGLT2 inhibitors attenuate endothelial to mesenchymal transition and cardiac fibroblast activation Article Snippet: One quarter of the of the cell suspension volume human anti-fibroblast Microbeads (Miltenyi Biotec) were added for 30 min at RT, light protected. .. Afterwards, two washing steps with 1 mL Article Title: Deletion of the Mitochondrial Membrane Protein Fam210b Is Associated with the Development of Systemic Lupus Erythematosus. Article Snippet: Then, 108 cells were resuspended in 500 μL MACS buffer with 10 μL FITC-conjugated anti-mouse CD71 antibody (Thermo Fisher Scientific Inc., Waltham, MA, USA) and incubated at 4–8 ◦C for 30 min. After, 10 mL PBS buffer was added to wash the cells. .. Each 107 cells were added with 90 μL Article Title: Dmxl1 Is an Essential Mammalian Gene that Is Required for V-ATPase Assembly and Function In Vivo. Article Snippet: A volume of 20 μL CD117/cit ma gnetic micr obeads (cat # 130-091-224, Miltenyi Biotec) per 0 7 cells were added, mixed well, and incubated for 15 min at ◦C with overhead rotation. .. A volume of 1 mL Immunolabeling:Article Title: Derivation and transcriptional reprogramming of border-forming wound repair astrocytes after spinal cord injury or stroke in mice Article Snippet: Nuclei were pelleted by centrifugation (model 5415R, Eppendorf; 500 r.p.m. for 5 min at 4 °C) and then resuspended in nuclei wash and resuspension buffer (1× PBS, 1% BSA, 0.2 U ul −1 RNase inhibitor) before being washed once more in nuclei wash and resuspension buffer and then filtered using a 5 ml polystyrene round-bottom tube with 35 μm cell-strainer cap and concentrated to a nuclei concentration of 1,000 nuclei per μl (1 × 10 6 nuclei per ml), resuspended and incubated with Sox9 rabbit monoclonal antibody (ThermoFisher, 72016) for 30 min and then centrifuged at 700× g for 10 min. Pellets were resuspended in 80 μl of MACS buffer composed of 1× PBS (tissue culture grade; Ca 2+ , Mg 2+ free), 0.5% nuclease-free BSA, and 2 mM EDTA, and then incubated with anti-Rabbit IgG Microbeads (Miltenyi, 130-048-602) for 20 min at 4 °C. .. After washing with 1 ml of Suspension:Article Title: SGLT2 inhibitors attenuate endothelial to mesenchymal transition and cardiac fibroblast activation. Article Snippet: One quarter of the of the cell suspension volume human anti-fibroblast Microbeads (Miltenyi Biotec) were added for 30 min at RT, light protected. .. Afterwards, two washing steps with 1 mL Article Title: SGLT2 inhibitors attenuate endothelial to mesenchymal transition and cardiac fibroblast activation Article Snippet: One quarter of the of the cell suspension volume human anti-fibroblast Microbeads (Miltenyi Biotec) were added for 30 min at RT, light protected. .. Afterwards, two washing steps with 1 mL Article Title: Dmxl1 Is an Essential Mammalian Gene that Is Required for V-ATPase Assembly and Function In Vivo. Article Snippet: A volume of 20 μL CD117/cit ma gnetic micr obeads (cat # 130-091-224, Miltenyi Biotec) per 0 7 cells were added, mixed well, and incubated for 15 min at ◦C with overhead rotation. .. A volume of 1 mL Incubation:Article Title: Synergy and antagonism in the integration of BCR and CD40 signals that control B-cell proliferation Article Snippet: .. Homogenized splenocytes were incubated with anti-CD43 magnetic beads for 15 min at 4-8°C, washed with Article Title: IL-2 and TCR stimulation induce expression and secretion of IL-32β by human T cells Article Snippet: To exclude dead cells, after washing with PBS (Sigma, Cat#D1408), the cells were stained with the Zombie NIRTM Fixable Viability Kit (BioLegend, Cat#423105) or the Zombie AquaTM Fixable Viability Kit (BioLegend, Cat#423102) according to the manufacturer’s recommendations. .. To analyze CD25 expression, cells were first washed with Article Title: Deletion of the Mitochondrial Membrane Protein Fam210b Is Associated with the Development of Systemic Lupus Erythematosus. Article Snippet: Then, 108 cells were resuspended in 500 μL MACS buffer with 10 μL FITC-conjugated anti-mouse CD71 antibody (Thermo Fisher Scientific Inc., Waltham, MA, USA) and incubated at 4–8 ◦C for 30 min. After, 10 mL PBS buffer was added to wash the cells. .. Each 107 cells were added with 90 μL Magnetic Beads:Article Title: Synergy and antagonism in the integration of BCR and CD40 signals that control B-cell proliferation Article Snippet: .. Homogenized splenocytes were incubated with anti-CD43 magnetic beads for 15 min at 4-8°C, washed with Article Title: Deletion of the Mitochondrial Membrane Protein Fam210b Is Associated with the Development of Systemic Lupus Erythematosus. Article Snippet: Then, 108 cells were resuspended in 500 μL MACS buffer with 10 μL FITC-conjugated anti-mouse CD71 antibody (Thermo Fisher Scientific Inc., Waltham, MA, USA) and incubated at 4–8 ◦C for 30 min. After, 10 mL PBS buffer was added to wash the cells. .. Each 107 cells were added with 90 μL Expressing:Article Title: IL-2 and TCR stimulation induce expression and secretion of IL-32β by human T cells Article Snippet: To exclude dead cells, after washing with PBS (Sigma, Cat#D1408), the cells were stained with the Zombie NIRTM Fixable Viability Kit (BioLegend, Cat#423105) or the Zombie AquaTM Fixable Viability Kit (BioLegend, Cat#423102) according to the manufacturer’s recommendations. .. To analyze CD25 expression, cells were first washed with Control:Article Title: IL-2 and TCR stimulation induce expression and secretion of IL-32β by human T cells Article Snippet: To exclude dead cells, after washing with PBS (Sigma, Cat#D1408), the cells were stained with the Zombie NIRTM Fixable Viability Kit (BioLegend, Cat#423105) or the Zombie AquaTM Fixable Viability Kit (BioLegend, Cat#423102) according to the manufacturer’s recommendations. .. To analyze CD25 expression, cells were first washed with |
