chemiluminescence imaging system (Clinx Science)
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Clinx Science
chemiluminescence imaging system
Chemiluminescence Imaging System, supplied by Clinx Science, used in various techniques. Bioz Stars score: 96/100, based on 322 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/machine/ChemiScope+6200+Touch+chemiluminescence+and+fluorescence+imaging+system/us12624019-425-68-71
Average 96 stars, based on 322 article reviews
Chemiluminescence Imaging System, supplied by Clinx Science, used in various techniques. Bioz Stars score: 96/100, based on 322 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/machine/ChemiScope+6200+Touch+chemiluminescence+and+fluorescence+imaging+system/us12624019-425-68-71
Average 96 stars, based on 322 article reviews
chemiluminescence imaging system - by Bioz Stars,
2026-10
96/100 stars
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Western Blot:Article Title: NDST3 suppression restores lysosomal acidification and ameliorates amyloid-β and MAPT/tau pathology in Alzheimer's disease. Article Snippet: The primary antibodies used in this study were as follows: ATP6V1A (#sc-293336, 1:500, Santa Cruz Biotechnology, Dallas, TX), ATP6V1C1 (#sc-271077, 1:500, Santa Cruz Biotechnology), ATP6V0D (#sc-393322, 1:500, Santa Cruz Biotechnology), LAMP2 (#BS61513, 1:1000, Bioworld, Bloomington, MN), 6E10 (#803015, 1:1000, Biolegend), APP (#ab32136, 1:1000, Abcam), APP-C-Terminal (#A8717, 1:1000, Sigma-Aldrich), GAPDH (#AF7021, 1:5000, Affinity), α-tubulin (#11224- 1-AP, 1:5000, Proteintech), MAPT/tau (#ab80579, 1:1000, Abcam), p-MAPT/tau (Ser262) (#AF3151, 1:1000, Affinity), p-MAPT/tau (Thr212) (#AF3146, 1:1000, Affinity), p-MAPT/tau(Ser422) (#GTX86147, 1:1000, GeneTex), β-actin (#AF7018, 1:5000, Affinity), HDAC6 (#AF6485, 1:1000, Affinity), LC3B (#ab192890, 1:2000, Abcam), SQSTM1/P62 (#5114, 1:1000, CST), GM130 (#HY-P82075, 1:1000, MCE), Calnexin (#HY-P80578, 1:1000, MCE), VDAC1 (#HY-P80369, 1:10000, MCE), and NDST3 (#NBP2-19501, 1:1000, Novus) at 4 °C for 12–16 h. After washing with PBS, the membrane was incubated with HRP-conjugated or IRDye-conjugated secondary antibody at room temperature for 1 h. The secondary antibodies used in this study were as follows: HRP-conjugated Goat Anti-Mouse IgG (#SA00001-1, 1:5000, Proteintech), HRP-conjugated Goat Anti-Rabbit IgG (#SA00001-2; 1:5000, Proteintech), IRDye 680LT donkey anti-mouse IgG secondary antibody (#926-68022, 1:10000, LI-COR, Lincoln, NE), and IRDye 800CW goat anti-rabbit IgG secondary antibody (#926–32211, 1:10000, LI-COR). .. Immunoblots were analyzed for band intensity using either ECL chemiluminescence substrate (#4AW011-500, Beijing 4A Biotech, Beijing, China) with Article Title: NDST3 suppression restores lysosomal acidification and ameliorates amyloid-β and MAPT/tau pathology in Alzheimer’s disease Article Snippet: The primary antibodies used in this study were as follows: ATP6V1A (#sc-293336, 1:500, Santa Cruz Biotechnology, Dallas, TX), ATP6V1C1 (#sc-271077, 1:500, Santa Cruz Biotechnology), ATP6V0D (#sc-393322, 1:500, Santa Cruz Biotechnology), LAMP2 (#BS61513, 1:1000, Bioworld, Bloomington, MN), 6E10 (#803015, 1:1000, Biolegend), APP (#ab32136, 1:1000, Abcam), APP-C-Terminal (#A8717, 1:1000, Sigma-Aldrich), GAPDH (#AF7021, 1:5000, Affinity), α-tubulin (#11224-1-AP, 1:5000, Proteintech), MAPT/tau (#ab80579, 1:1000, Abcam), p-MAPT/tau (Ser262) (#AF3151, 1:1000, Affinity), p-MAPT/tau (Thr212) (#AF3146, 1:1000, Affinity), p-MAPT/tau(Ser422) (#GTX86147, 1:1000, GeneTex), β-actin (#AF7018, 1:5000, Affinity), HDAC6 (#AF6485, 1:1000, Affinity), LC3B (#ab192890, 1:2000, Abcam), SQSTM1/P62 (#5114, 1:1000, CST), GM130 (#HY- P82075 , 1:1000, MCE), Calnexin (#HY- P80578 , 1:1000, MCE), VDAC1 (#HY- P80369 , 1:10000, MCE), and NDST3 (#NBP2-19501, 1:1000, Novus) at 4 °C for 12–16 h. After washing with PBS, the membrane was incubated with HRP-conjugated or IRDye-conjugated secondary antibody at room temperature for 1 h. The secondary antibodies used in this study were as follows: HRP-conjugated Goat Anti-Mouse IgG (#SA00001-1, 1:5000, Proteintech), HRP-conjugated Goat Anti-Rabbit IgG (#SA00001-2; 1:5000, Proteintech), IRDye 680LT donkey anti-mouse IgG secondary antibody (#926-68022, 1:10000, LI-COR, Lincoln, NE), and IRDye 800CW goat anti-rabbit IgG secondary antibody (#926–32211, 1:10000, LI-COR). .. Immunoblots were analyzed for band intensity using either ECL chemiluminescence substrate (#4AW011-500, Beijing 4A Biotech, Beijing, China) with Article Title: Indazole derivative, and preparation method therefor and use thereof Article Snippet: After the membrane was transferred, the membrane was added in 1×QuickBlock blocking buffer (Beyotime) and incubated for 30 minutes at room temperature. .. After blocking, PVDF membranes were incubated with IRAK4 primary antibody (Abcam) overnight at 4°, the membranes were washed with TBST buffer (2.4 g Tris, 8.8 g NaCl, 1.5 mL Tween 20, pH adjusted to 7.4, constant volume to 1 L) for 30 minutes, incubated with secondary antibody (Abcam) for 2 hours at room temperature, finally incubated with Clarity Western ECL Substrate (BIO-RAD) for 5 minutes for luminescence development, Imaging:Article Title: NDST3 suppression restores lysosomal acidification and ameliorates amyloid-β and MAPT/tau pathology in Alzheimer's disease. Article Snippet: The primary antibodies used in this study were as follows: ATP6V1A (#sc-293336, 1:500, Santa Cruz Biotechnology, Dallas, TX), ATP6V1C1 (#sc-271077, 1:500, Santa Cruz Biotechnology), ATP6V0D (#sc-393322, 1:500, Santa Cruz Biotechnology), LAMP2 (#BS61513, 1:1000, Bioworld, Bloomington, MN), 6E10 (#803015, 1:1000, Biolegend), APP (#ab32136, 1:1000, Abcam), APP-C-Terminal (#A8717, 1:1000, Sigma-Aldrich), GAPDH (#AF7021, 1:5000, Affinity), α-tubulin (#11224- 1-AP, 1:5000, Proteintech), MAPT/tau (#ab80579, 1:1000, Abcam), p-MAPT/tau (Ser262) (#AF3151, 1:1000, Affinity), p-MAPT/tau (Thr212) (#AF3146, 1:1000, Affinity), p-MAPT/tau(Ser422) (#GTX86147, 1:1000, GeneTex), β-actin (#AF7018, 1:5000, Affinity), HDAC6 (#AF6485, 1:1000, Affinity), LC3B (#ab192890, 1:2000, Abcam), SQSTM1/P62 (#5114, 1:1000, CST), GM130 (#HY-P82075, 1:1000, MCE), Calnexin (#HY-P80578, 1:1000, MCE), VDAC1 (#HY-P80369, 1:10000, MCE), and NDST3 (#NBP2-19501, 1:1000, Novus) at 4 °C for 12–16 h. After washing with PBS, the membrane was incubated with HRP-conjugated or IRDye-conjugated secondary antibody at room temperature for 1 h. The secondary antibodies used in this study were as follows: HRP-conjugated Goat Anti-Mouse IgG (#SA00001-1, 1:5000, Proteintech), HRP-conjugated Goat Anti-Rabbit IgG (#SA00001-2; 1:5000, Proteintech), IRDye 680LT donkey anti-mouse IgG secondary antibody (#926-68022, 1:10000, LI-COR, Lincoln, NE), and IRDye 800CW goat anti-rabbit IgG secondary antibody (#926–32211, 1:10000, LI-COR). .. Immunoblots were analyzed for band intensity using either ECL chemiluminescence substrate (#4AW011-500, Beijing 4A Biotech, Beijing, China) with Article Title: NDST3 suppression restores lysosomal acidification and ameliorates amyloid-β and MAPT/tau pathology in Alzheimer’s disease Article Snippet: The primary antibodies used in this study were as follows: ATP6V1A (#sc-293336, 1:500, Santa Cruz Biotechnology, Dallas, TX), ATP6V1C1 (#sc-271077, 1:500, Santa Cruz Biotechnology), ATP6V0D (#sc-393322, 1:500, Santa Cruz Biotechnology), LAMP2 (#BS61513, 1:1000, Bioworld, Bloomington, MN), 6E10 (#803015, 1:1000, Biolegend), APP (#ab32136, 1:1000, Abcam), APP-C-Terminal (#A8717, 1:1000, Sigma-Aldrich), GAPDH (#AF7021, 1:5000, Affinity), α-tubulin (#11224-1-AP, 1:5000, Proteintech), MAPT/tau (#ab80579, 1:1000, Abcam), p-MAPT/tau (Ser262) (#AF3151, 1:1000, Affinity), p-MAPT/tau (Thr212) (#AF3146, 1:1000, Affinity), p-MAPT/tau(Ser422) (#GTX86147, 1:1000, GeneTex), β-actin (#AF7018, 1:5000, Affinity), HDAC6 (#AF6485, 1:1000, Affinity), LC3B (#ab192890, 1:2000, Abcam), SQSTM1/P62 (#5114, 1:1000, CST), GM130 (#HY- P82075 , 1:1000, MCE), Calnexin (#HY- P80578 , 1:1000, MCE), VDAC1 (#HY- P80369 , 1:10000, MCE), and NDST3 (#NBP2-19501, 1:1000, Novus) at 4 °C for 12–16 h. After washing with PBS, the membrane was incubated with HRP-conjugated or IRDye-conjugated secondary antibody at room temperature for 1 h. The secondary antibodies used in this study were as follows: HRP-conjugated Goat Anti-Mouse IgG (#SA00001-1, 1:5000, Proteintech), HRP-conjugated Goat Anti-Rabbit IgG (#SA00001-2; 1:5000, Proteintech), IRDye 680LT donkey anti-mouse IgG secondary antibody (#926-68022, 1:10000, LI-COR, Lincoln, NE), and IRDye 800CW goat anti-rabbit IgG secondary antibody (#926–32211, 1:10000, LI-COR). .. Immunoblots were analyzed for band intensity using either ECL chemiluminescence substrate (#4AW011-500, Beijing 4A Biotech, Beijing, China) with Article Title: Indazole derivative, and preparation method therefor and use thereof Article Snippet: After the membrane was transferred, the membrane was added in 1×QuickBlock blocking buffer (Beyotime) and incubated for 30 minutes at room temperature. .. After blocking, PVDF membranes were incubated with IRAK4 primary antibody (Abcam) overnight at 4°, the membranes were washed with TBST buffer (2.4 g Tris, 8.8 g NaCl, 1.5 mL Tween 20, pH adjusted to 7.4, constant volume to 1 L) for 30 minutes, incubated with secondary antibody (Abcam) for 2 hours at room temperature, finally incubated with Clarity Western ECL Substrate (BIO-RAD) for 5 minutes for luminescence development, Electrophoresis:Article Title: A protease-free colorimetric strategy based on an aptamer and DCHA-mediated cyclic amplification for the ultrasensitive biosensing of Escherichia coli Article Snippet: Herein, a novel protease-free colorimetric strategy was proposed for the ultrasensitive biosensing of Escherichia coli (E. coli, strain CMCC 44102).. This strategy employed an E. coli whole-cell aptamer for specific recognition and dual-catalyst hairpin assembly (DCHA)-mediated cyclic amplification.. Specifically, four nucleic acid strands (Apt, H1, H2, and S) mediated DCHA cyclic amplification, while the G-quadruplex/hemin complex acted as a DNAzyme to catalyze the oxidation of ABTS2− for signal generation. Expressing:Article Title: Alterations in the structural properties of polysaccharides in garlic yellow seeds before and after germination and their mechanism for alleviating colitis. Article Snippet: Garlic yellow are cultivated from bulbs under dark conditions, with cloves as natural seeds.. This study explored the structural properties of differences between pre-germination garlic polysaccharides (GP) and postgermination garlic sprout polysaccharides (GSP) from garlic yellow and evaluated their effects on DSSinduced colitis mice.. The results indicate germination induced glycosidic bond cleavage in GP, generating shorter chains and low-molecular-weight GSP with altered crystallinity and solubility. Software:Article Title: Alterations in the structural properties of polysaccharides in garlic yellow seeds before and after germination and their mechanism for alleviating colitis. Article Snippet: Garlic yellow are cultivated from bulbs under dark conditions, with cloves as natural seeds.. This study explored the structural properties of differences between pre-germination garlic polysaccharides (GP) and postgermination garlic sprout polysaccharides (GSP) from garlic yellow and evaluated their effects on DSSinduced colitis mice.. The results indicate germination induced glycosidic bond cleavage in GP, generating shorter chains and low-molecular-weight GSP with altered crystallinity and solubility. Blocking Assay:Article Title: Indazole derivative, and preparation method therefor and use thereof Article Snippet: After the membrane was transferred, the membrane was added in 1×QuickBlock blocking buffer (Beyotime) and incubated for 30 minutes at room temperature. .. After blocking, PVDF membranes were incubated with IRAK4 primary antibody (Abcam) overnight at 4°, the membranes were washed with TBST buffer (2.4 g Tris, 8.8 g NaCl, 1.5 mL Tween 20, pH adjusted to 7.4, constant volume to 1 L) for 30 minutes, incubated with secondary antibody (Abcam) for 2 hours at room temperature, finally incubated with Clarity Western ECL Substrate (BIO-RAD) for 5 minutes for luminescence development, Incubation:Article Title: Indazole derivative, and preparation method therefor and use thereof Article Snippet: After the membrane was transferred, the membrane was added in 1×QuickBlock blocking buffer (Beyotime) and incubated for 30 minutes at room temperature. .. After blocking, PVDF membranes were incubated with IRAK4 primary antibody (Abcam) overnight at 4°, the membranes were washed with TBST buffer (2.4 g Tris, 8.8 g NaCl, 1.5 mL Tween 20, pH adjusted to 7.4, constant volume to 1 L) for 30 minutes, incubated with secondary antibody (Abcam) for 2 hours at room temperature, finally incubated with Clarity Western ECL Substrate (BIO-RAD) for 5 minutes for luminescence development, other:Article Title: Discovery of a potent and orally available SOS1 inhibitor with antitumor efficacy in KRAS-mutant colorectal cancers. Article Snippet: As a key guanine nucleotide exchange factor, SOS1 is an attractive therapeutic target for KRAS-driven colorectal cancer.. In this study, based on the co-crystal structure of SOS1 in complex with BI-68BS, we developed a highaffinity fluorescent tracer TRR2 (KD = 0.134 μM), and established a robust fluorescence polarization assay for the profiling of SOS1 inhibitors.. Subsequent structure-based optimization yielded a series of SOS1 inhibitors, among which SL43 emerged as the most promising candidate. |