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clinx chemiluminescence system  (Clinx Science)


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    Structured Review

    Clinx Science clinx chemiluminescence system
    Clinx Chemiluminescence System, supplied by Clinx Science, used in various techniques. Bioz Stars score: 96/100, based on 3830 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/machine/ChemiScope+6100+chemiluminescence+and+fluorescence+imaging+system/pm41329132-69-31-34
    Average 96 stars, based on 3830 article reviews
    clinx chemiluminescence system - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Post-Exposure 3-Methyladenine Is Associated with Lower Indices of Hepatic Injury and Fibrotic Remodeling After Chronic Sodium Arsenite Exposure in Mice
    Article Snippet: .. Bands were visualized using enhanced chemiluminescence and captured with a ChemiScope 6100 imaging system (Clinx Science Instruments, Shanghai, China). .. Band intensities were quantified using AlphaEaseFC version 3.1.2 (Alpha Innotech, San Leandro, CA, USA).

    Article Title: Desflurane Increases Hippocampal RAF-MAPK-MSK1 Protein Levels and Reduces CLOCK Expression: Sorafenib Attenuates Cognitive Impairment in Aged Mice.
    Article Snippet: After three 10-min washes with 1× TBST, membranes were incubated with HRP-conjugated secondary antibodies (goat anti-rabbit IgG or goat anti-mouse IgG, 1:3000; Saierbio, China) for 1 h at room temperature. .. Bands were visualized by enhanced chemiluminescence (ECL; CLINX, China) using a ChemiScope 6100 imaging system (CLINX, China). ..

    Article Title: HUCMSCs-Derived Exosomes Attenuate Keloid Fibroblast Pathological Phenotypes with Associated Metabolic-Redox-Matrix Network Remodeling: A Proteomic and In Vivo Study.
    Article Snippet: .. ZetaView PMX 110 (Particle Metrix) for NTA; epifluorescence microscope BX53 and confocal microscope FV3000 (Olympus); flow cytometer CytoFLEX (Beckman Coulter); qPCR system ViiA7 (Applied Biosystems); microplate reader BK-EL10C (BIOBASE); chemiluminescence imaging system CLINX 6100; CO 2 incubator BPN- 150CH(UV) (Shanghai Yiheng); microvolume spectrophotometer ND-100 (Hangzhou MIUO). .. Magnetic-bead digestion kit MMB-96 (Qinglian Bio); high-performance liquid chromatography (HPLC) system RIGOL L-3000 (Rigol) coupled to an Orbitrap Eclipse mass spectrometer with high-field asymmetric waveform ion mobility spectrometry (FAIMS) Pro (Thermo Fisher).

    Article Title: Expression of V‑domain Ig suppressor of T cell activation in ovarian cancer and its regulation of follicular regulatory T‑cell function via the AKT/MAPK pathway.
    Article Snippet: After rinsing with TBST, the membrane was incubated with primary antibodies separately: anti‐β‐actin (SinoBiological, cat. no. 100166‐MM10, 1:1,000 dilution) and anti‐VISTA (Abcam; cat. no. ab300042, 1:1,000 dilution) at 4 ̊C overnight. .. After rinsing with TBST, the corresponding secondary antibodies were added: Goat anti‐mouse IgG H&L (HRP) (Abcam; cat. no. ab205719, 1:10,000 dilution) and goat anti‐rabbit IgG H&L (HRP) (Abcam; cat. no. ab205718, 1:5,000 dilution), followed by incubation at room temperature for 1 h. After rinsing with TBST, the membrane was devel‐ oped with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, Inc.; cat. no. 34580), and imaged using a ChemiScope 3000 chemiluminescence imaging system (Clinx Science Instruments Co., Ltd.). .. For quantitative analysis, Band intensities were measured by manually drawing rectangular regions of interest around each target band in ImageJ, band gray value quantification was completed using ImageJ software v1.54f (National Institutes of Health); total protein levels were normalized to β‐actin as the loading control; relative phosphorylation levels of AKT and ERK1/2 were calculated as the ratio of phosphorylated (p‐) protein to the corresponding total protein (p‐AKT/total AKT, p‐ERK1/2/total ERK1/2).

    Article Title: Identification and Functional Characterization of Of-FADD in Ostrinia furnacalis Infected with Bacteria.
    Article Snippet: 30 Fas-associated protein with death domain (FADD) is a crucial adaptor protein in the insect 31 Immune Deficiency (IMD) signaling pathway.. Although its role in regulating antimicrobial 32 peptide (AMP) synthesis has been established in Drosophila, its function in Ostrinia furnacalis 33 remains unclear.. In this study, we identified a FADD (designated Of-FADD) with a full-length 34 cDNA of 971 bp, containing a 666 bp open reading frame encoding a 221-residue protein with 35 a characteristic death effector domain (DED) and a death domain (DD).

    Article Title: Chemosensory protein BmCSP4 enhances silkworm adaptation to mulberry by suppressing plant defense responses.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Microscopy:

    Article Title: HUCMSCs-Derived Exosomes Attenuate Keloid Fibroblast Pathological Phenotypes with Associated Metabolic-Redox-Matrix Network Remodeling: A Proteomic and In Vivo Study.
    Article Snippet: .. ZetaView PMX 110 (Particle Metrix) for NTA; epifluorescence microscope BX53 and confocal microscope FV3000 (Olympus); flow cytometer CytoFLEX (Beckman Coulter); qPCR system ViiA7 (Applied Biosystems); microplate reader BK-EL10C (BIOBASE); chemiluminescence imaging system CLINX 6100; CO 2 incubator BPN- 150CH(UV) (Shanghai Yiheng); microvolume spectrophotometer ND-100 (Hangzhou MIUO). .. Magnetic-bead digestion kit MMB-96 (Qinglian Bio); high-performance liquid chromatography (HPLC) system RIGOL L-3000 (Rigol) coupled to an Orbitrap Eclipse mass spectrometer with high-field asymmetric waveform ion mobility spectrometry (FAIMS) Pro (Thermo Fisher).

    Confocal:

    Article Title: HUCMSCs-Derived Exosomes Attenuate Keloid Fibroblast Pathological Phenotypes with Associated Metabolic-Redox-Matrix Network Remodeling: A Proteomic and In Vivo Study.
    Article Snippet: .. ZetaView PMX 110 (Particle Metrix) for NTA; epifluorescence microscope BX53 and confocal microscope FV3000 (Olympus); flow cytometer CytoFLEX (Beckman Coulter); qPCR system ViiA7 (Applied Biosystems); microplate reader BK-EL10C (BIOBASE); chemiluminescence imaging system CLINX 6100; CO 2 incubator BPN- 150CH(UV) (Shanghai Yiheng); microvolume spectrophotometer ND-100 (Hangzhou MIUO). .. Magnetic-bead digestion kit MMB-96 (Qinglian Bio); high-performance liquid chromatography (HPLC) system RIGOL L-3000 (Rigol) coupled to an Orbitrap Eclipse mass spectrometer with high-field asymmetric waveform ion mobility spectrometry (FAIMS) Pro (Thermo Fisher).

    Flow Cytometry:

    Article Title: HUCMSCs-Derived Exosomes Attenuate Keloid Fibroblast Pathological Phenotypes with Associated Metabolic-Redox-Matrix Network Remodeling: A Proteomic and In Vivo Study.
    Article Snippet: .. ZetaView PMX 110 (Particle Metrix) for NTA; epifluorescence microscope BX53 and confocal microscope FV3000 (Olympus); flow cytometer CytoFLEX (Beckman Coulter); qPCR system ViiA7 (Applied Biosystems); microplate reader BK-EL10C (BIOBASE); chemiluminescence imaging system CLINX 6100; CO 2 incubator BPN- 150CH(UV) (Shanghai Yiheng); microvolume spectrophotometer ND-100 (Hangzhou MIUO). .. Magnetic-bead digestion kit MMB-96 (Qinglian Bio); high-performance liquid chromatography (HPLC) system RIGOL L-3000 (Rigol) coupled to an Orbitrap Eclipse mass spectrometer with high-field asymmetric waveform ion mobility spectrometry (FAIMS) Pro (Thermo Fisher).

    Real-time Polymerase Chain Reaction:

    Article Title: HUCMSCs-Derived Exosomes Attenuate Keloid Fibroblast Pathological Phenotypes with Associated Metabolic-Redox-Matrix Network Remodeling: A Proteomic and In Vivo Study.
    Article Snippet: .. ZetaView PMX 110 (Particle Metrix) for NTA; epifluorescence microscope BX53 and confocal microscope FV3000 (Olympus); flow cytometer CytoFLEX (Beckman Coulter); qPCR system ViiA7 (Applied Biosystems); microplate reader BK-EL10C (BIOBASE); chemiluminescence imaging system CLINX 6100; CO 2 incubator BPN- 150CH(UV) (Shanghai Yiheng); microvolume spectrophotometer ND-100 (Hangzhou MIUO). .. Magnetic-bead digestion kit MMB-96 (Qinglian Bio); high-performance liquid chromatography (HPLC) system RIGOL L-3000 (Rigol) coupled to an Orbitrap Eclipse mass spectrometer with high-field asymmetric waveform ion mobility spectrometry (FAIMS) Pro (Thermo Fisher).

    Incubator:

    Article Title: HUCMSCs-Derived Exosomes Attenuate Keloid Fibroblast Pathological Phenotypes with Associated Metabolic-Redox-Matrix Network Remodeling: A Proteomic and In Vivo Study.
    Article Snippet: .. ZetaView PMX 110 (Particle Metrix) for NTA; epifluorescence microscope BX53 and confocal microscope FV3000 (Olympus); flow cytometer CytoFLEX (Beckman Coulter); qPCR system ViiA7 (Applied Biosystems); microplate reader BK-EL10C (BIOBASE); chemiluminescence imaging system CLINX 6100; CO 2 incubator BPN- 150CH(UV) (Shanghai Yiheng); microvolume spectrophotometer ND-100 (Hangzhou MIUO). .. Magnetic-bead digestion kit MMB-96 (Qinglian Bio); high-performance liquid chromatography (HPLC) system RIGOL L-3000 (Rigol) coupled to an Orbitrap Eclipse mass spectrometer with high-field asymmetric waveform ion mobility spectrometry (FAIMS) Pro (Thermo Fisher).

    Spectrophotometry:

    Article Title: HUCMSCs-Derived Exosomes Attenuate Keloid Fibroblast Pathological Phenotypes with Associated Metabolic-Redox-Matrix Network Remodeling: A Proteomic and In Vivo Study.
    Article Snippet: .. ZetaView PMX 110 (Particle Metrix) for NTA; epifluorescence microscope BX53 and confocal microscope FV3000 (Olympus); flow cytometer CytoFLEX (Beckman Coulter); qPCR system ViiA7 (Applied Biosystems); microplate reader BK-EL10C (BIOBASE); chemiluminescence imaging system CLINX 6100; CO 2 incubator BPN- 150CH(UV) (Shanghai Yiheng); microvolume spectrophotometer ND-100 (Hangzhou MIUO). .. Magnetic-bead digestion kit MMB-96 (Qinglian Bio); high-performance liquid chromatography (HPLC) system RIGOL L-3000 (Rigol) coupled to an Orbitrap Eclipse mass spectrometer with high-field asymmetric waveform ion mobility spectrometry (FAIMS) Pro (Thermo Fisher).

    Incubation:

    Article Title: Expression of V‑domain Ig suppressor of T cell activation in ovarian cancer and its regulation of follicular regulatory T‑cell function via the AKT/MAPK pathway.
    Article Snippet: After rinsing with TBST, the membrane was incubated with primary antibodies separately: anti‐β‐actin (SinoBiological, cat. no. 100166‐MM10, 1:1,000 dilution) and anti‐VISTA (Abcam; cat. no. ab300042, 1:1,000 dilution) at 4 ̊C overnight. .. After rinsing with TBST, the corresponding secondary antibodies were added: Goat anti‐mouse IgG H&L (HRP) (Abcam; cat. no. ab205719, 1:10,000 dilution) and goat anti‐rabbit IgG H&L (HRP) (Abcam; cat. no. ab205718, 1:5,000 dilution), followed by incubation at room temperature for 1 h. After rinsing with TBST, the membrane was devel‐ oped with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, Inc.; cat. no. 34580), and imaged using a ChemiScope 3000 chemiluminescence imaging system (Clinx Science Instruments Co., Ltd.). .. For quantitative analysis, Band intensities were measured by manually drawing rectangular regions of interest around each target band in ImageJ, band gray value quantification was completed using ImageJ software v1.54f (National Institutes of Health); total protein levels were normalized to β‐actin as the loading control; relative phosphorylation levels of AKT and ERK1/2 were calculated as the ratio of phosphorylated (p‐) protein to the corresponding total protein (p‐AKT/total AKT, p‐ERK1/2/total ERK1/2).

    Membrane:

    Article Title: Expression of V‑domain Ig suppressor of T cell activation in ovarian cancer and its regulation of follicular regulatory T‑cell function via the AKT/MAPK pathway.
    Article Snippet: After rinsing with TBST, the membrane was incubated with primary antibodies separately: anti‐β‐actin (SinoBiological, cat. no. 100166‐MM10, 1:1,000 dilution) and anti‐VISTA (Abcam; cat. no. ab300042, 1:1,000 dilution) at 4 ̊C overnight. .. After rinsing with TBST, the corresponding secondary antibodies were added: Goat anti‐mouse IgG H&L (HRP) (Abcam; cat. no. ab205719, 1:10,000 dilution) and goat anti‐rabbit IgG H&L (HRP) (Abcam; cat. no. ab205718, 1:5,000 dilution), followed by incubation at room temperature for 1 h. After rinsing with TBST, the membrane was devel‐ oped with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, Inc.; cat. no. 34580), and imaged using a ChemiScope 3000 chemiluminescence imaging system (Clinx Science Instruments Co., Ltd.). .. For quantitative analysis, Band intensities were measured by manually drawing rectangular regions of interest around each target band in ImageJ, band gray value quantification was completed using ImageJ software v1.54f (National Institutes of Health); total protein levels were normalized to β‐actin as the loading control; relative phosphorylation levels of AKT and ERK1/2 were calculated as the ratio of phosphorylated (p‐) protein to the corresponding total protein (p‐AKT/total AKT, p‐ERK1/2/total ERK1/2).

    Staining:

    Article Title: SMURF1 alleviates preeclampsia by suppressing ferroptosis in trophoblast cells via mediating ubiquitination of FLT1.
    Article Snippet: Background: Ferroptosis, an emerging form of regulated cell death, and ubiquitination have not been fully clarified in preeclampsia (PE).. Aberrant overex ression of Fms-like tyrosine kinase 1 (FLT1) is closely associated with PE development, yet the association between FLT1 and ferroptosis in PE, as well as the involvement of ubiquitination in this process, remains unexplored.. Methods: To recapitulate PE pathophysiology, rat models were generated via N(G)-Nitro-Larginine methyl ester (L-NAME) administration, and HTR-8/Svneo cells were subjected to hypoxic stress.

    Western Blot:

    Article Title: Identification and Functional Characterization of Of-FADD in Ostrinia furnacalis Infected with Bacteria.
    Article Snippet: 30 Fas-associated protein with death domain (FADD) is a crucial adaptor protein in the insect 31 Immune Deficiency (IMD) signaling pathway.. Although its role in regulating antimicrobial 32 peptide (AMP) synthesis has been established in Drosophila, its function in Ostrinia furnacalis 33 remains unclear.. In this study, we identified a FADD (designated Of-FADD) with a full-length 34 cDNA of 971 bp, containing a 666 bp open reading frame encoding a 221-residue protein with 35 a characteristic death effector domain (DED) and a death domain (DD).



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