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afp amerlex-m ria detection kit  (Quidel)


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    Quidel afp amerlex-m ria detection kit
    Afp Amerlex M Ria Detection Kit, supplied by Quidel, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m+detection+kit/afp+amerlex+m+ria+detection+kit/pmc03282982-55-10-15
    Average 90 stars, based on 1 article reviews
    afp amerlex-m ria detection kit - by Bioz Stars, 2026-11
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    Article Title: A therapeutic method for the direct reprogramming of human liver cancer cells with only chemicals
    Article Snippet: The quantitative analysis of AFP secretion was performed with the AFP Amerlex-M RIA detection kit (Ortho Clinical Diagnostics, Issy les Moulineaux, France).



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    Effects of propofol intervention on oxidative stress and inflammatory factors in H/R‐stimulated cardiomyocytes. (A) Intracellular malondialdehyde (MDA) levels in H9c2 cells of each group determined by colorimetric kits. (B) ELISA detection of superoxide dismutase (SOD) expression in cells of each group. (C) Relative reactive oxygen species (ROS) contents in cells of each group measured using fluorescent probes. (D) ELISA quantification of tumor necrosis factor‐ α (TNF‐ α ) concentrations in cell culture supernatant of each group. (E) ELISA detection of interleukin‐6 (IL‐6) concentrations in cell culture supernatant of each group. (F) ELISA measurement of interleukin‐1 β (IL‐1 β ) concentrations in cell culture supernatant of each group. ∗∗ p < 0.01; ∗∗∗ p < 0.001.

    Journal: Cardiovascular Therapeutics

    Article Title: The miR‐511‐3p/ATP2A2 Axis Is Required for Propofol‐Mediated Cardioprotection Against Hypoxia/Reoxygenation Injury

    doi: 10.1155/cdr/7021184

    Figure Lengend Snippet: Effects of propofol intervention on oxidative stress and inflammatory factors in H/R‐stimulated cardiomyocytes. (A) Intracellular malondialdehyde (MDA) levels in H9c2 cells of each group determined by colorimetric kits. (B) ELISA detection of superoxide dismutase (SOD) expression in cells of each group. (C) Relative reactive oxygen species (ROS) contents in cells of each group measured using fluorescent probes. (D) ELISA quantification of tumor necrosis factor‐ α (TNF‐ α ) concentrations in cell culture supernatant of each group. (E) ELISA detection of interleukin‐6 (IL‐6) concentrations in cell culture supernatant of each group. (F) ELISA measurement of interleukin‐1 β (IL‐1 β ) concentrations in cell culture supernatant of each group. ∗∗ p < 0.01; ∗∗∗ p < 0.001.

    Article Snippet: Colorimetric quantitative analysis was performed with lactate dehydrogenase (LDH) assay kits (Abcam, United Kingdom) and malondialdehyde (MDA) detection kits (Elabscience, China).

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Cell Culture

    Knocking down HDAC4 inhibits glutamine metabolism in A549 cells. (A) Glutamine uptake levels in A549 cells. (B) Glutamate production levels in A549 cells. (C) α‐Ketoglutarate production levels in A549 cells. (D) Measurement of GLS activity in A549 cells. (E) DCFH‐DA staining was used to measure the level of ROS in cells. (F) A kit was used to measure the GSH/GSSG ratio. (G) Western blotting was used to detect the expression of Nrf2, HO‐1, and NQO1. ∗∗ p < 0.01, ∗∗∗ p < 0.001. Data are presented as mean ± SD. All experiments were performed with three independent biological replicates. Statistical analysis was conducted using Student’s t ‐test for panels (A)‒(G). GLS, glutaminase; DCFH‐DA, 2 ′ ,7 ′ dichlorodihydrofluorescein diacetate; ROS, reactive oxygen species; GSH, reduced glutathione; GSSG, oxidized glutathione; Nrf2, nuclear factor erythroid 2 related factor 2; HO‐1, heme oxygenase 1; NQO1: NAD(P)H quinone dehydrogenase 1. The original, uncropped western blot images for Figure are provided in the Supporting Information.

    Journal: Analytical Cellular Pathology (Amsterdam)

    Article Title: HDAC4 Promotes Cancer Stemness in Nonsmall Cell Lung Cancer by Upregulating SLC38A2 Expression via HIF‐1α to Enhance Glutamine Metabolism

    doi: 10.1155/ancp/2525721

    Figure Lengend Snippet: Knocking down HDAC4 inhibits glutamine metabolism in A549 cells. (A) Glutamine uptake levels in A549 cells. (B) Glutamate production levels in A549 cells. (C) α‐Ketoglutarate production levels in A549 cells. (D) Measurement of GLS activity in A549 cells. (E) DCFH‐DA staining was used to measure the level of ROS in cells. (F) A kit was used to measure the GSH/GSSG ratio. (G) Western blotting was used to detect the expression of Nrf2, HO‐1, and NQO1. ∗∗ p < 0.01, ∗∗∗ p < 0.001. Data are presented as mean ± SD. All experiments were performed with three independent biological replicates. Statistical analysis was conducted using Student’s t ‐test for panels (A)‒(G). GLS, glutaminase; DCFH‐DA, 2 ′ ,7 ′ dichlorodihydrofluorescein diacetate; ROS, reactive oxygen species; GSH, reduced glutathione; GSSG, oxidized glutathione; Nrf2, nuclear factor erythroid 2 related factor 2; HO‐1, heme oxygenase 1; NQO1: NAD(P)H quinone dehydrogenase 1. The original, uncropped western blot images for Figure are provided in the Supporting Information.

    Article Snippet: The levels of total glutathione (T‐GSH) and GSSG in A549 cells were measured using a GSH/GSSG ratio detection kit (E‐BC‐K097‐M, Elabscience, China) following the manufacturer’s instructions.

    Techniques: Activity Assay, Staining, Western Blot, Expressing, Analysis