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iron concentration detection kits  (Elabscience Biotechnology)


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    Structured Review

    Elabscience Biotechnology iron concentration detection kits
    Iron Concentration Detection Kits, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 652 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m+detection+kit/Ferrous+Iron+Colorimetric+Assay+Kit/pm41947221-89-0-22
    Average 97 stars, based on 652 article reviews
    iron concentration detection kits - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    ROS Assay:

    Article Title: Xieyu Jiedu Granules attenuate sepsis-associated acute kidney injury through ESR1: An integrative study combining transcriptomics, network pharmacology, machine learning, and molecular docking
    Article Snippet: The concentrations of TNF-α and IL-6 were assayed in serum, rat kidney tissues, and treated HK-2 cells using corresponding Rat and Human ELISA Kits (Enzyme-linked Biotechnology, Shanghai, China), in accordance with the accompanying suggestions. .. The levels of key ferroptosis markers were measured in rat kidney tissues and treated HK-2 cells using commercial kits as per vendors’ instructions: ROS was assessed with a ROS Assay Kit (Elabscience, Wuhan, China), Fe2+ with a Ferrous Iron Colorimetric Assay Kit (Elabscience), MDA with an Enhanced MDA Assay Kit (Beyotime), and GSH with a GSH Assay Kit (Abcam). .. Rat kidney tissues and treated HK-2 cells were lysed in buffer containing protease and phosphatase inhibitors (Beyotime) for protein extraction.

    Colorimetric Assay:

    Article Title: Xieyu Jiedu Granules attenuate sepsis-associated acute kidney injury through ESR1: An integrative study combining transcriptomics, network pharmacology, machine learning, and molecular docking
    Article Snippet: The concentrations of TNF-α and IL-6 were assayed in serum, rat kidney tissues, and treated HK-2 cells using corresponding Rat and Human ELISA Kits (Enzyme-linked Biotechnology, Shanghai, China), in accordance with the accompanying suggestions. .. The levels of key ferroptosis markers were measured in rat kidney tissues and treated HK-2 cells using commercial kits as per vendors’ instructions: ROS was assessed with a ROS Assay Kit (Elabscience, Wuhan, China), Fe2+ with a Ferrous Iron Colorimetric Assay Kit (Elabscience), MDA with an Enhanced MDA Assay Kit (Beyotime), and GSH with a GSH Assay Kit (Abcam). .. Rat kidney tissues and treated HK-2 cells were lysed in buffer containing protease and phosphatase inhibitors (Beyotime) for protein extraction.

    Article Title: Targeting PPARγ-p53 to reprogram intervertebral disc degeneration through ferroptosis and senescence modulation
    Article Snippet: .. Ferrous iron assay in NPCs: the intracellular concentration of ferrous iron was quantified using commercial colorimetric assay kits (Elabscience, Wuhan, China). ..

    Article Title: The Berberine Derivative BBR684 Inhibits VDAC Oligomerization to Suppress Ferroptosis in Acute Kidney Injury
    Article Snippet: .. Intracellular Fe2+ levels were quantified using a Ferrous Iron Colorimetric Assay Kit (Catalog #E-BC-K773-M, Elabscience, Wuhan, China) per the manufacturer's protocol. ..

    Multiple Displacement Amplification:

    Article Title: Xieyu Jiedu Granules attenuate sepsis-associated acute kidney injury through ESR1: An integrative study combining transcriptomics, network pharmacology, machine learning, and molecular docking
    Article Snippet: The concentrations of TNF-α and IL-6 were assayed in serum, rat kidney tissues, and treated HK-2 cells using corresponding Rat and Human ELISA Kits (Enzyme-linked Biotechnology, Shanghai, China), in accordance with the accompanying suggestions. .. The levels of key ferroptosis markers were measured in rat kidney tissues and treated HK-2 cells using commercial kits as per vendors’ instructions: ROS was assessed with a ROS Assay Kit (Elabscience, Wuhan, China), Fe2+ with a Ferrous Iron Colorimetric Assay Kit (Elabscience), MDA with an Enhanced MDA Assay Kit (Beyotime), and GSH with a GSH Assay Kit (Abcam). .. Rat kidney tissues and treated HK-2 cells were lysed in buffer containing protease and phosphatase inhibitors (Beyotime) for protein extraction.

    Article Title: Mechanistic insights into TLR2-mediated acute pancreatitis exacerbation via macrophage-ferroptotic acinar cell crosstalk.
    Article Snippet: For 459 pharmacological inhibition or activation of ferroptosis, mice were intraperitoneally injected with 460 liproxstatin-1 (Lip-1, HY-12726, and MCE) at a dose of 10 mg/kg body weight before AP induction. .. 461 ELISA 462 The concentrations or activities of amylase (ab102523; Abcam), lipase (ab102524; Abcam), 463 lactate dehydrogenase (E-BC-K771-M, Elabscience), GSH (E-BC-K097-M, Elabscience), PTGS2 464 (E-EL-M0959-M, Elabscience), iron (E-BC-K773-M, Elabscience), MDA (E-BC-K025-M, 465 Elabscience), TNF-α (E-EL-M3063, Elabscience), IL-1β (E-EL-M0037, Elabscience), and IL-6 466 (E-MSEL-M0001, Elabscience) in the indicated samples were measured using commercial ELISA kits 467 according to the manufacturer’s instruction. ..

    Article Title: HFPO-TA inhibits testosterone synthesis by triggering FTO-mediated m 6 A modification to drive NCOA4-associated ferroptosis.
    Article Snippet: Libraries were constructed and sequenced on the Illumina NovaSeq 6000 platform (LC-Bio Technology, China) with three biological replicates per group. .. Levels of MDA (Elabscience, E-BC-K025-M and E-BC-K028-M), GSH (Elabscience, E-BC-K030-M), and serum Fe2+ (Elabscience, E-BC-K773M) were measured using commercial kits following the manufacturer’s instructions. .. Denatured RNA was spotted onto a HybondN + membrane (GE Healthcare, RPN303B) and UV-crosslinked.

    GSH Assay:

    Article Title: Xieyu Jiedu Granules attenuate sepsis-associated acute kidney injury through ESR1: An integrative study combining transcriptomics, network pharmacology, machine learning, and molecular docking
    Article Snippet: The concentrations of TNF-α and IL-6 were assayed in serum, rat kidney tissues, and treated HK-2 cells using corresponding Rat and Human ELISA Kits (Enzyme-linked Biotechnology, Shanghai, China), in accordance with the accompanying suggestions. .. The levels of key ferroptosis markers were measured in rat kidney tissues and treated HK-2 cells using commercial kits as per vendors’ instructions: ROS was assessed with a ROS Assay Kit (Elabscience, Wuhan, China), Fe2+ with a Ferrous Iron Colorimetric Assay Kit (Elabscience), MDA with an Enhanced MDA Assay Kit (Beyotime), and GSH with a GSH Assay Kit (Abcam). .. Rat kidney tissues and treated HK-2 cells were lysed in buffer containing protease and phosphatase inhibitors (Beyotime) for protein extraction.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Mechanistic insights into TLR2-mediated acute pancreatitis exacerbation via macrophage-ferroptotic acinar cell crosstalk.
    Article Snippet: For 459 pharmacological inhibition or activation of ferroptosis, mice were intraperitoneally injected with 460 liproxstatin-1 (Lip-1, HY-12726, and MCE) at a dose of 10 mg/kg body weight before AP induction. .. 461 ELISA 462 The concentrations or activities of amylase (ab102523; Abcam), lipase (ab102524; Abcam), 463 lactate dehydrogenase (E-BC-K771-M, Elabscience), GSH (E-BC-K097-M, Elabscience), PTGS2 464 (E-EL-M0959-M, Elabscience), iron (E-BC-K773-M, Elabscience), MDA (E-BC-K025-M, 465 Elabscience), TNF-α (E-EL-M3063, Elabscience), IL-1β (E-EL-M0037, Elabscience), and IL-6 466 (E-MSEL-M0001, Elabscience) in the indicated samples were measured using commercial ELISA kits 467 according to the manufacturer’s instruction. ..

    Iron Assay:

    Article Title: Targeting PPARγ-p53 to reprogram intervertebral disc degeneration through ferroptosis and senescence modulation
    Article Snippet: .. Ferrous iron assay in NPCs: the intracellular concentration of ferrous iron was quantified using commercial colorimetric assay kits (Elabscience, Wuhan, China). ..

    Concentration Assay:

    Article Title: Targeting PPARγ-p53 to reprogram intervertebral disc degeneration through ferroptosis and senescence modulation
    Article Snippet: .. Ferrous iron assay in NPCs: the intracellular concentration of ferrous iron was quantified using commercial colorimetric assay kits (Elabscience, Wuhan, China). ..

    other:

    Article Title: Spermidine-functionalized Janus hydrogel microneedles inhibit ferroptosis and promote healing of oral ulcers
    Article Snippet: The BCA protein assay kit (PC0020, Solarbio) enabled quantification of protein levels.



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    Effects of propofol intervention on oxidative stress and inflammatory factors in H/R‐stimulated cardiomyocytes. (A) Intracellular <t>malondialdehyde</t> <t>(MDA)</t> levels in H9c2 cells of each group determined by colorimetric kits. (B) ELISA detection of superoxide dismutase (SOD) expression in cells of each group. (C) Relative reactive oxygen species (ROS) contents in cells of each group measured using fluorescent probes. (D) ELISA quantification of tumor necrosis factor‐ α (TNF‐ α ) concentrations in cell culture supernatant of each group. (E) ELISA detection of interleukin‐6 (IL‐6) concentrations in cell culture supernatant of each group. (F) ELISA measurement of interleukin‐1 β (IL‐1 β ) concentrations in cell culture supernatant of each group. ∗∗ p < 0.01; ∗∗∗ p < 0.001.
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    Effects of propofol intervention on oxidative stress and inflammatory factors in H/R‐stimulated cardiomyocytes. (A) Intracellular <t>malondialdehyde</t> <t>(MDA)</t> levels in H9c2 cells of each group determined by colorimetric kits. (B) ELISA detection of superoxide dismutase (SOD) expression in cells of each group. (C) Relative reactive oxygen species (ROS) contents in cells of each group measured using fluorescent probes. (D) ELISA quantification of tumor necrosis factor‐ α (TNF‐ α ) concentrations in cell culture supernatant of each group. (E) ELISA detection of interleukin‐6 (IL‐6) concentrations in cell culture supernatant of each group. (F) ELISA measurement of interleukin‐1 β (IL‐1 β ) concentrations in cell culture supernatant of each group. ∗∗ p < 0.01; ∗∗∗ p < 0.001.
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    Knocking down HDAC4 inhibits glutamine metabolism in A549 cells. (A) Glutamine uptake levels in A549 cells. (B) Glutamate production levels in A549 cells. (C) α‐Ketoglutarate production levels in A549 cells. (D) Measurement of GLS activity in A549 cells. (E) DCFH‐DA staining was used to measure the level of ROS in cells. (F) A kit was used to measure <t>the</t> <t>GSH/GSSG</t> ratio. (G) Western blotting was used to detect the expression of Nrf2, HO‐1, and NQO1. ∗∗ p < 0.01, ∗∗∗ p < 0.001. Data are presented as mean ± SD. All experiments were performed with three independent biological replicates. Statistical analysis was conducted using Student’s t ‐test for panels (A)‒(G). GLS, glutaminase; DCFH‐DA, 2 ′ ,7 ′ dichlorodihydrofluorescein diacetate; ROS, reactive oxygen species; GSH, reduced glutathione; GSSG, oxidized glutathione; Nrf2, nuclear factor erythroid 2 related factor 2; HO‐1, heme oxygenase 1; NQO1: NAD(P)H quinone dehydrogenase 1. The original, uncropped western blot images for Figure are provided in the Supporting Information.
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    Knocking down HDAC4 inhibits glutamine metabolism in A549 cells. (A) Glutamine uptake levels in A549 cells. (B) Glutamate production levels in A549 cells. (C) α‐Ketoglutarate production levels in A549 cells. (D) Measurement of GLS activity in A549 cells. (E) DCFH‐DA staining was used to measure the level of ROS in cells. (F) A kit was used to measure <t>the</t> <t>GSH/GSSG</t> ratio. (G) Western blotting was used to detect the expression of Nrf2, HO‐1, and NQO1. ∗∗ p < 0.01, ∗∗∗ p < 0.001. Data are presented as mean ± SD. All experiments were performed with three independent biological replicates. Statistical analysis was conducted using Student’s t ‐test for panels (A)‒(G). GLS, glutaminase; DCFH‐DA, 2 ′ ,7 ′ dichlorodihydrofluorescein diacetate; ROS, reactive oxygen species; GSH, reduced glutathione; GSSG, oxidized glutathione; Nrf2, nuclear factor erythroid 2 related factor 2; HO‐1, heme oxygenase 1; NQO1: NAD(P)H quinone dehydrogenase 1. The original, uncropped western blot images for Figure are provided in the Supporting Information.
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    Knocking down HDAC4 inhibits glutamine metabolism in A549 cells. (A) Glutamine uptake levels in A549 cells. (B) Glutamate production levels in A549 cells. (C) α‐Ketoglutarate production levels in A549 cells. (D) Measurement of GLS activity in A549 cells. (E) DCFH‐DA staining was used to measure the level of ROS in cells. (F) A kit was used to measure <t>the</t> <t>GSH/GSSG</t> ratio. (G) Western blotting was used to detect the expression of Nrf2, HO‐1, and NQO1. ∗∗ p < 0.01, ∗∗∗ p < 0.001. Data are presented as mean ± SD. All experiments were performed with three independent biological replicates. Statistical analysis was conducted using Student’s t ‐test for panels (A)‒(G). GLS, glutaminase; DCFH‐DA, 2 ′ ,7 ′ dichlorodihydrofluorescein diacetate; ROS, reactive oxygen species; GSH, reduced glutathione; GSSG, oxidized glutathione; Nrf2, nuclear factor erythroid 2 related factor 2; HO‐1, heme oxygenase 1; NQO1: NAD(P)H quinone dehydrogenase 1. The original, uncropped western blot images for Figure are provided in the Supporting Information.
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    Knocking down HDAC4 inhibits glutamine metabolism in A549 cells. (A) Glutamine uptake levels in A549 cells. (B) Glutamate production levels in A549 cells. (C) α‐Ketoglutarate production levels in A549 cells. (D) Measurement of GLS activity in A549 cells. (E) DCFH‐DA staining was used to measure the level of ROS in cells. (F) A kit was used to measure <t>the</t> <t>GSH/GSSG</t> ratio. (G) Western blotting was used to detect the expression of Nrf2, HO‐1, and NQO1. ∗∗ p < 0.01, ∗∗∗ p < 0.001. Data are presented as mean ± SD. All experiments were performed with three independent biological replicates. Statistical analysis was conducted using Student’s t ‐test for panels (A)‒(G). GLS, glutaminase; DCFH‐DA, 2 ′ ,7 ′ dichlorodihydrofluorescein diacetate; ROS, reactive oxygen species; GSH, reduced glutathione; GSSG, oxidized glutathione; Nrf2, nuclear factor erythroid 2 related factor 2; HO‐1, heme oxygenase 1; NQO1: NAD(P)H quinone dehydrogenase 1. The original, uncropped western blot images for Figure are provided in the Supporting Information.
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    Knocking down HDAC4 inhibits glutamine metabolism in A549 cells. (A) Glutamine uptake levels in A549 cells. (B) Glutamate production levels in A549 cells. (C) α‐Ketoglutarate production levels in A549 cells. (D) Measurement of GLS activity in A549 cells. (E) DCFH‐DA staining was used to measure the level of ROS in cells. (F) A kit was used to measure <t>the</t> <t>GSH/GSSG</t> ratio. (G) Western blotting was used to detect the expression of Nrf2, HO‐1, and NQO1. ∗∗ p < 0.01, ∗∗∗ p < 0.001. Data are presented as mean ± SD. All experiments were performed with three independent biological replicates. Statistical analysis was conducted using Student’s t ‐test for panels (A)‒(G). GLS, glutaminase; DCFH‐DA, 2 ′ ,7 ′ dichlorodihydrofluorescein diacetate; ROS, reactive oxygen species; GSH, reduced glutathione; GSSG, oxidized glutathione; Nrf2, nuclear factor erythroid 2 related factor 2; HO‐1, heme oxygenase 1; NQO1: NAD(P)H quinone dehydrogenase 1. The original, uncropped western blot images for Figure are provided in the Supporting Information.
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    Image Search Results


    Effects of propofol intervention on oxidative stress and inflammatory factors in H/R‐stimulated cardiomyocytes. (A) Intracellular malondialdehyde (MDA) levels in H9c2 cells of each group determined by colorimetric kits. (B) ELISA detection of superoxide dismutase (SOD) expression in cells of each group. (C) Relative reactive oxygen species (ROS) contents in cells of each group measured using fluorescent probes. (D) ELISA quantification of tumor necrosis factor‐ α (TNF‐ α ) concentrations in cell culture supernatant of each group. (E) ELISA detection of interleukin‐6 (IL‐6) concentrations in cell culture supernatant of each group. (F) ELISA measurement of interleukin‐1 β (IL‐1 β ) concentrations in cell culture supernatant of each group. ∗∗ p < 0.01; ∗∗∗ p < 0.001.

    Journal: Cardiovascular Therapeutics

    Article Title: The miR‐511‐3p/ATP2A2 Axis Is Required for Propofol‐Mediated Cardioprotection Against Hypoxia/Reoxygenation Injury

    doi: 10.1155/cdr/7021184

    Figure Lengend Snippet: Effects of propofol intervention on oxidative stress and inflammatory factors in H/R‐stimulated cardiomyocytes. (A) Intracellular malondialdehyde (MDA) levels in H9c2 cells of each group determined by colorimetric kits. (B) ELISA detection of superoxide dismutase (SOD) expression in cells of each group. (C) Relative reactive oxygen species (ROS) contents in cells of each group measured using fluorescent probes. (D) ELISA quantification of tumor necrosis factor‐ α (TNF‐ α ) concentrations in cell culture supernatant of each group. (E) ELISA detection of interleukin‐6 (IL‐6) concentrations in cell culture supernatant of each group. (F) ELISA measurement of interleukin‐1 β (IL‐1 β ) concentrations in cell culture supernatant of each group. ∗∗ p < 0.01; ∗∗∗ p < 0.001.

    Article Snippet: Colorimetric quantitative analysis was performed with lactate dehydrogenase (LDH) assay kits (Abcam, United Kingdom) and malondialdehyde (MDA) detection kits (Elabscience, China).

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Cell Culture

    Knocking down HDAC4 inhibits glutamine metabolism in A549 cells. (A) Glutamine uptake levels in A549 cells. (B) Glutamate production levels in A549 cells. (C) α‐Ketoglutarate production levels in A549 cells. (D) Measurement of GLS activity in A549 cells. (E) DCFH‐DA staining was used to measure the level of ROS in cells. (F) A kit was used to measure the GSH/GSSG ratio. (G) Western blotting was used to detect the expression of Nrf2, HO‐1, and NQO1. ∗∗ p < 0.01, ∗∗∗ p < 0.001. Data are presented as mean ± SD. All experiments were performed with three independent biological replicates. Statistical analysis was conducted using Student’s t ‐test for panels (A)‒(G). GLS, glutaminase; DCFH‐DA, 2 ′ ,7 ′ dichlorodihydrofluorescein diacetate; ROS, reactive oxygen species; GSH, reduced glutathione; GSSG, oxidized glutathione; Nrf2, nuclear factor erythroid 2 related factor 2; HO‐1, heme oxygenase 1; NQO1: NAD(P)H quinone dehydrogenase 1. The original, uncropped western blot images for Figure are provided in the Supporting Information.

    Journal: Analytical Cellular Pathology (Amsterdam)

    Article Title: HDAC4 Promotes Cancer Stemness in Nonsmall Cell Lung Cancer by Upregulating SLC38A2 Expression via HIF‐1α to Enhance Glutamine Metabolism

    doi: 10.1155/ancp/2525721

    Figure Lengend Snippet: Knocking down HDAC4 inhibits glutamine metabolism in A549 cells. (A) Glutamine uptake levels in A549 cells. (B) Glutamate production levels in A549 cells. (C) α‐Ketoglutarate production levels in A549 cells. (D) Measurement of GLS activity in A549 cells. (E) DCFH‐DA staining was used to measure the level of ROS in cells. (F) A kit was used to measure the GSH/GSSG ratio. (G) Western blotting was used to detect the expression of Nrf2, HO‐1, and NQO1. ∗∗ p < 0.01, ∗∗∗ p < 0.001. Data are presented as mean ± SD. All experiments were performed with three independent biological replicates. Statistical analysis was conducted using Student’s t ‐test for panels (A)‒(G). GLS, glutaminase; DCFH‐DA, 2 ′ ,7 ′ dichlorodihydrofluorescein diacetate; ROS, reactive oxygen species; GSH, reduced glutathione; GSSG, oxidized glutathione; Nrf2, nuclear factor erythroid 2 related factor 2; HO‐1, heme oxygenase 1; NQO1: NAD(P)H quinone dehydrogenase 1. The original, uncropped western blot images for Figure are provided in the Supporting Information.

    Article Snippet: The levels of total glutathione (T‐GSH) and GSSG in A549 cells were measured using a GSH/GSSG ratio detection kit (E‐BC‐K097‐M, Elabscience, China) following the manufacturer’s instructions.

    Techniques: Activity Assay, Staining, Western Blot, Expressing, Analysis