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lysis solution  (MACHEREY NAGEL)


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    MACHEREY NAGEL lysis solution
    Lysis Solution, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lysis+buffer+p+solution/Lysis+Buffer+P/pmc11748876-46-1-18
    Average 93 stars, based on 41 article reviews
    lysis solution - by Bioz Stars, 2026-11
    93/100 stars

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    Isolation:

    Article Title: Characterisation of the noble crayfish immune response to oomycete-derived immunostimulants.
    Article Snippet: .. Total RNA from haemolymph cell pellet was isolated using the NuceloSpin 31 Jo urn al Pr e-p roo f RNA Mini kit (Macherey-Nagel, Germany) according to the manufacturers’ protocol with the 1 following adjustments: 150 μL Buffer RA1 was used for cell lysis; 150 μL ethanol was used to 2 adjust the RNA binding conditions; 300 μL Buffer RA3 was used for the second wash; instead 3 of the third wash, a drying step was added in which the empty column was centrifuged for 2 4 min at 11000 x g; RNA was eluted two times in the same microtube using 30 μL of RNase-free 5 H2O each time. .. RNA quality was assessed on the NanoVue Spectrophotometer, and RNA 6 quantity with the QuantiFluor RNA System on a Quantus fluorometer (Promega, USA). cDNA 7 synthesis was performed using the iScriptTM cDNA Synthesis Kit (Bio-Rad, USA) according 8 to the manufacturers’ protocol, using half volume reactions and prolonging the reverse 9 transcription step of the protocol to 40 min. Target loci for the qPCR were CCAAT/enhancer-10 binding protein (C/EBP), Krüppel homolog-1 (Kr-h1) and prophenoloxidase (proPO), while 11 elongation factor one alpha (EF1α) was used as a reference housekeeping gene.

    Lysis:

    Article Title: Characterisation of the noble crayfish immune response to oomycete-derived immunostimulants.
    Article Snippet: .. Total RNA from haemolymph cell pellet was isolated using the NuceloSpin 31 Jo urn al Pr e-p roo f RNA Mini kit (Macherey-Nagel, Germany) according to the manufacturers’ protocol with the 1 following adjustments: 150 μL Buffer RA1 was used for cell lysis; 150 μL ethanol was used to 2 adjust the RNA binding conditions; 300 μL Buffer RA3 was used for the second wash; instead 3 of the third wash, a drying step was added in which the empty column was centrifuged for 2 4 min at 11000 x g; RNA was eluted two times in the same microtube using 30 μL of RNase-free 5 H2O each time. .. RNA quality was assessed on the NanoVue Spectrophotometer, and RNA 6 quantity with the QuantiFluor RNA System on a Quantus fluorometer (Promega, USA). cDNA 7 synthesis was performed using the iScriptTM cDNA Synthesis Kit (Bio-Rad, USA) according 8 to the manufacturers’ protocol, using half volume reactions and prolonging the reverse 9 transcription step of the protocol to 40 min. Target loci for the qPCR were CCAAT/enhancer-10 binding protein (C/EBP), Krüppel homolog-1 (Kr-h1) and prophenoloxidase (proPO), while 11 elongation factor one alpha (EF1α) was used as a reference housekeeping gene.

    Article Title: An autoregulatory poison exon in Smndc1 is conserved across kingdoms and influences organism growth
    Article Snippet: .. To genotype founder mice and subsequent generations, ear tissue was collected when the mice were two to three weeks old and digested in Lysis M Buffer (Macherey-Nagel) and Proteinase K (Qiagen) for five minutes at room temperature. ..

    Article Title: Autonomous Development and Regeneration of Isolated Rice Egg Cells in Vitro in a Fertilization-Independent Manner.
    Article Snippet: an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints.. All other permissions can be obtained through our RightsLink service via the Permissions l ink on the article page on our site—for further information please contact journals.permissions@oup.com.

    Article Title: Key wound healing genes as diagnostic biomarkers and therapeutic targets in uterine corpus endometrial carcinoma: an integrated in silico and in vitro study
    Article Snippet: Briefly, the cells were washed with 1 mL of pre-chilled 1× phosphate-buffered saline (PBS) and lysed in 100 μL of NP40 buffer [50 mM Tris–HCl pH 7.5, 10 mM MgCl 2 , 100 mM NaCl, 10% glycerol, and 1% Nonidet P-40 (Roche)] containing RNase inhibitor (NZYTech, Portugal). .. The lysis solution was then collected, and total RNA was extracted using the Nucleospin RNA extraction II Kit (Macherey–Nagel, Düren, Germany) or 500 μL TRIzolTM, following the manufacturer’s protocol. .. RNA concentrations were measured with a NanoDrop1000 spectrophotometer (Thermo Fisher Scientific).

    RNA Binding Assay:

    Article Title: Characterisation of the noble crayfish immune response to oomycete-derived immunostimulants.
    Article Snippet: .. Total RNA from haemolymph cell pellet was isolated using the NuceloSpin 31 Jo urn al Pr e-p roo f RNA Mini kit (Macherey-Nagel, Germany) according to the manufacturers’ protocol with the 1 following adjustments: 150 μL Buffer RA1 was used for cell lysis; 150 μL ethanol was used to 2 adjust the RNA binding conditions; 300 μL Buffer RA3 was used for the second wash; instead 3 of the third wash, a drying step was added in which the empty column was centrifuged for 2 4 min at 11000 x g; RNA was eluted two times in the same microtube using 30 μL of RNase-free 5 H2O each time. .. RNA quality was assessed on the NanoVue Spectrophotometer, and RNA 6 quantity with the QuantiFluor RNA System on a Quantus fluorometer (Promega, USA). cDNA 7 synthesis was performed using the iScriptTM cDNA Synthesis Kit (Bio-Rad, USA) according 8 to the manufacturers’ protocol, using half volume reactions and prolonging the reverse 9 transcription step of the protocol to 40 min. Target loci for the qPCR were CCAAT/enhancer-10 binding protein (C/EBP), Krüppel homolog-1 (Kr-h1) and prophenoloxidase (proPO), while 11 elongation factor one alpha (EF1α) was used as a reference housekeeping gene.

    RNA Extraction:

    Article Title: Key wound healing genes as diagnostic biomarkers and therapeutic targets in uterine corpus endometrial carcinoma: an integrated in silico and in vitro study
    Article Snippet: Briefly, the cells were washed with 1 mL of pre-chilled 1× phosphate-buffered saline (PBS) and lysed in 100 μL of NP40 buffer [50 mM Tris–HCl pH 7.5, 10 mM MgCl 2 , 100 mM NaCl, 10% glycerol, and 1% Nonidet P-40 (Roche)] containing RNase inhibitor (NZYTech, Portugal). .. The lysis solution was then collected, and total RNA was extracted using the Nucleospin RNA extraction II Kit (Macherey–Nagel, Düren, Germany) or 500 μL TRIzolTM, following the manufacturer’s protocol. .. RNA concentrations were measured with a NanoDrop1000 spectrophotometer (Thermo Fisher Scientific).



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