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Serumwerk Bernburg AG lymphoprep density gradient solution
Lymphoprep Density Gradient Solution, supplied by Serumwerk Bernburg AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lymphoprep+density+gradient+solution/lymphoprep+density+gradient+solution/pm39514524-75-13-17
Average 90 stars, based on 1 article reviews
lymphoprep density gradient solution - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Isolation:

Article Title: Failing maternal-fetal tolerance in SLE (FaMaLE): a prospective cohort study for finding the molecular mechanisms behind pregnancy complications.
Article Snippet: .. Dankers W, et al. Lupus Science & Medicine 2025;12:e001668. doi:10.1136/lupus-2025-001668 5 then PBMCs are isolated within 24 hours from the heparin tubes, using Lymphoprep (Serumwerk, Bernburg, Germany) density gradient centrifugation. .. 2–4 million PBMCs are analysed using flow cytometry, and remaining PBMCs are frozen per 5–10 million cells in freezing medium (10% dimethyl sulfoxide (DMSO) (VWR, Amsterdam, The Netherlands) and 90% Iscove’s Modified Dulbecco’s Medium (Gibco) supplemented with 30% FCS, 0.05 mg/mL gentamicin (Gibco) and 0.00036 (v/v)% β-mercaptoethanol.

Article Title: Differential metabolic pathways underlie THC- and CBD-mediated inhibition of B-cell activation in both young and aged mice
Article Snippet: .. Splenocytes were centrifuged using a Lymphoprep gradient (Serumwerk Bernburg AG, Bernburg, Germany), and mononuclear cells were isolated from the interphase layer. .. A total of 1 × 10 6 splenocytes/well were plated in 24-well flat-bottom plates with RPMI 1640 medium supplemented with 10% fetal calf serum (FCS), 1% penicillin/streptomycin, and 1% L-glutamine (Sartorius, Beit Haemek, Israel).

Article Title: Failing maternal–fetal tolerance in SLE (FaMaLE): a prospective cohort study for finding the molecular mechanisms behind pregnancy complications
Article Snippet: .. 100 μL of whole blood is saved for analysis using flow cytometry, and then PBMCs are isolated within 24 hours from the heparin tubes, using Lymphoprep (Serumwerk, Bernburg, Germany) density gradient centrifugation. .. 2–4 million PBMCs are analysed using flow cytometry, and remaining PBMCs are frozen per 5–10 million cells in freezing medium (10% dimethyl sulfoxide (DMSO) (VWR, Amsterdam, The Netherlands) and 90% Iscove’s Modified Dulbecco’s Medium (Gibco) supplemented with 30% FCS, 0.05 mg/mL gentamicin (Gibco) and 0.00036 (v/v)% β-mercaptoethanol.

Article Title: Ex vivo pembrolizumab pharmacology for personalized PD-1 inhibitor therapy reveals a critical gap between receptor occupancy and T cell functionality.
Article Snippet: Purpose: Targeting the programmed death (ligand)-1 (PD-1/PD-L1) axis with immune checkpoint inhibitors (ICIs), like pembrolizumab, has improved cancer survival.. Unfortunately, the optimal dose remains unknown and less than 50 % of patients respond.. Understanding PD-1 receptor pharmacology and developing early-response biomarkers are crucial to personalize therapy.

Gradient Centrifugation:

Article Title: Failing maternal-fetal tolerance in SLE (FaMaLE): a prospective cohort study for finding the molecular mechanisms behind pregnancy complications.
Article Snippet: .. Dankers W, et al. Lupus Science & Medicine 2025;12:e001668. doi:10.1136/lupus-2025-001668 5 then PBMCs are isolated within 24 hours from the heparin tubes, using Lymphoprep (Serumwerk, Bernburg, Germany) density gradient centrifugation. .. 2–4 million PBMCs are analysed using flow cytometry, and remaining PBMCs are frozen per 5–10 million cells in freezing medium (10% dimethyl sulfoxide (DMSO) (VWR, Amsterdam, The Netherlands) and 90% Iscove’s Modified Dulbecco’s Medium (Gibco) supplemented with 30% FCS, 0.05 mg/mL gentamicin (Gibco) and 0.00036 (v/v)% β-mercaptoethanol.

Article Title: Failing maternal–fetal tolerance in SLE (FaMaLE): a prospective cohort study for finding the molecular mechanisms behind pregnancy complications
Article Snippet: .. 100 μL of whole blood is saved for analysis using flow cytometry, and then PBMCs are isolated within 24 hours from the heparin tubes, using Lymphoprep (Serumwerk, Bernburg, Germany) density gradient centrifugation. .. 2–4 million PBMCs are analysed using flow cytometry, and remaining PBMCs are frozen per 5–10 million cells in freezing medium (10% dimethyl sulfoxide (DMSO) (VWR, Amsterdam, The Netherlands) and 90% Iscove’s Modified Dulbecco’s Medium (Gibco) supplemented with 30% FCS, 0.05 mg/mL gentamicin (Gibco) and 0.00036 (v/v)% β-mercaptoethanol.

Article Title: Ex vivo pembrolizumab pharmacology for personalized PD-1 inhibitor therapy reveals a critical gap between receptor occupancy and T cell functionality.
Article Snippet: Purpose: Targeting the programmed death (ligand)-1 (PD-1/PD-L1) axis with immune checkpoint inhibitors (ICIs), like pembrolizumab, has improved cancer survival.. Unfortunately, the optimal dose remains unknown and less than 50 % of patients respond.. Understanding PD-1 receptor pharmacology and developing early-response biomarkers are crucial to personalize therapy.

other:

Article Title: Intracellular and exosomal localization of the negative checkpoint regulator VISTA in immune cells.
Article Snippet: To isolate peripheral blood mononuclear cells (PBMCs), the interphase of a Lymphoprep (Serumwerk Bernburg AG, Germany) gradient was isolated, and the cell amount was quantified via flow cytometry (Accuri® C6, BD Biosciences, California, USA).

Flow Cytometry:

Article Title: Failing maternal–fetal tolerance in SLE (FaMaLE): a prospective cohort study for finding the molecular mechanisms behind pregnancy complications
Article Snippet: .. 100 μL of whole blood is saved for analysis using flow cytometry, and then PBMCs are isolated within 24 hours from the heparin tubes, using Lymphoprep (Serumwerk, Bernburg, Germany) density gradient centrifugation. .. 2–4 million PBMCs are analysed using flow cytometry, and remaining PBMCs are frozen per 5–10 million cells in freezing medium (10% dimethyl sulfoxide (DMSO) (VWR, Amsterdam, The Netherlands) and 90% Iscove’s Modified Dulbecco’s Medium (Gibco) supplemented with 30% FCS, 0.05 mg/mL gentamicin (Gibco) and 0.00036 (v/v)% β-mercaptoethanol.



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