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lunascript rt supermix kit  (New England Biolabs)


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    Structured Review

    New England Biolabs lunascript rt supermix kit
    Lunascript Rt Supermix Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1833 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lunascript+rt+superscript+kit/LunaScript+RT+SuperMix+Kit/pmc10746192-376-16-20
    Average 99 stars, based on 1833 article reviews
    lunascript rt supermix kit - by Bioz Stars, 2026-09
    99/100 stars

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    Synthesized:

    Article Title: Quantitative expression of estrogen, progesterone and human epidermal growth factor receptor-2 and their correlation with immunohistochemistry in breast cancer at Uganda Cancer Institute
    Article Snippet: .. Complementary DNA (cDNA) was synthesized using a LunaScript RT SuperMix Kit (E3010S) (New England BioLabs, Hitchin, England) according to the manufacturer’s instructions. ..

    Article Title: Quantitative proteomics reveals extensive lysine ubiquitination and transcription factor stability states in Arabidopsis
    Article Snippet: .. Total RNA was extracted with Trizol (Invitrogen) and Direct-zol RNA MiniPrep kit (Zymo Research). cDNA was synthesized with LunaScript RT SuperMix Kit (NEB). .. Quantitative PCR was done with the following program: 3 min at 95 °C, followed by 39 cycles of 10 s at 95 °C, 10 s at 60 °C, and 30 s at 72 °C with a CFX96 TM Real-Time system (Bio-Rad).

    cDNA Synthesis:

    Article Title: Enhanced RNAi does not provide efficient innate antiviral immunity in mice
    Article Snippet: For Dicer expression analysis, aliquots of 3 μg of total RNA were treated by a TURBO DNA-freeTM Kit (Invitrogen) according to the manufacturer’s instructions. .. Next, aliquots of 1 μg of total DNAse-treated RNA were used for complementary DNA (cDNA) synthesis by a LunaScript RT SuperMix Kit (New England Biolabs) according to the manufacturer’s instructions. ..

    Reverse Transcription:

    Article Title: RBPscan: A Quantitative, In Vivo Tool for Profiling RNA-Binding Protein Interactions
    Article Snippet: .. Reverse transcription was performed with 1 μg of total RNA in 20 □L of total volume using the LunaScript RT SuperMix Kit (NEB #E3010) under the following conditions: 2 minutes at 25°C, 10 minutes at 55°C, and 1 minute at 95°C. ..

    Article Title: Paracrine role of endothelial IGF-1 receptor in depot-specific adipose tissue adaptation in male mice
    Article Snippet: RNA was isolated from tissue and cells samples (NEB, T2010S). .. The concentration of RNA in each sample (ng/ul) was measured using a Nanodrop. cDNA was reverse transcribed from the RNA samples (NEB, E3010L). .. Quantitative PCR (qPCR) was performed using a Roche LightCycler 480 Instrument II, using SYBR Green PCR Master Mix (Bio-Rad, 1725120) and relevant primers (Supp Table ).

    Real-time Polymerase Chain Reaction:

    Article Title: Pantothenate kinase 4 controls skeletal muscle substrate metabolism
    Article Snippet: RNA from tissues was isolated by Trizol-Chloroform (peqGOLD TriFastTM, 30-2010P, VWR) extraction in homogenized tissues and precipitation with Isopropanol. .. Genomic DNA was removed (DNAse I, EN0521, Fisher Scientific; RiboLock RNAse Inhibitor, EO0382, Fisher Scientific) and 1 μg RNA was transcribed into cDNA using LunaScript RT Super Mix (E3010L, New England Biolabs). mRNA levels were determined by qPCR using SYBR Green (Luna Universal qPCR Master Mix, M3003E, New England Biolabs) on a VIA7 Real-Time PCR System (4453534, Thermo Fischer Scientific). ..

    Article Title: Pantothenate kinase 4 controls skeletal muscle substrate metabolism.
    Article Snippet: RNA from tissues was isolated by Trizol-Chloroform (peqGOLD TriFastTM, 30-2010P, VWR) extraction in homogenized tissues and precipitation with Isopropanol. .. Genomic DNAwas removed (DNAse I, EN0521, Fisher Scientific; RiboLock RNAse Inhibitor, EO0382, Fisher Scientific) and 1 μg RNA was transcribed into cDNA using LunaScript RT Super Mix (E3010L, New England Biolabs). mRNA levels were determined by qPCR using SYBR Green (Luna Universal qPCRMasterMix,M3003E,NewEnglandBiolabs) on aVIA7RealTime PCR System (4453534, Thermo Fischer Scientific). ..

    SYBR Green Assay:

    Article Title: Pantothenate kinase 4 controls skeletal muscle substrate metabolism
    Article Snippet: RNA from tissues was isolated by Trizol-Chloroform (peqGOLD TriFastTM, 30-2010P, VWR) extraction in homogenized tissues and precipitation with Isopropanol. .. Genomic DNA was removed (DNAse I, EN0521, Fisher Scientific; RiboLock RNAse Inhibitor, EO0382, Fisher Scientific) and 1 μg RNA was transcribed into cDNA using LunaScript RT Super Mix (E3010L, New England Biolabs). mRNA levels were determined by qPCR using SYBR Green (Luna Universal qPCR Master Mix, M3003E, New England Biolabs) on a VIA7 Real-Time PCR System (4453534, Thermo Fischer Scientific). ..

    Article Title: Pantothenate kinase 4 controls skeletal muscle substrate metabolism.
    Article Snippet: RNA from tissues was isolated by Trizol-Chloroform (peqGOLD TriFastTM, 30-2010P, VWR) extraction in homogenized tissues and precipitation with Isopropanol. .. Genomic DNAwas removed (DNAse I, EN0521, Fisher Scientific; RiboLock RNAse Inhibitor, EO0382, Fisher Scientific) and 1 μg RNA was transcribed into cDNA using LunaScript RT Super Mix (E3010L, New England Biolabs). mRNA levels were determined by qPCR using SYBR Green (Luna Universal qPCRMasterMix,M3003E,NewEnglandBiolabs) on aVIA7RealTime PCR System (4453534, Thermo Fischer Scientific). ..

    Concentration Assay:

    Article Title: Paracrine role of endothelial IGF-1 receptor in depot-specific adipose tissue adaptation in male mice
    Article Snippet: RNA was isolated from tissue and cells samples (NEB, T2010S). .. The concentration of RNA in each sample (ng/ul) was measured using a Nanodrop. cDNA was reverse transcribed from the RNA samples (NEB, E3010L). .. Quantitative PCR (qPCR) was performed using a Roche LightCycler 480 Instrument II, using SYBR Green PCR Master Mix (Bio-Rad, 1725120) and relevant primers (Supp Table ).

    Polymerase Chain Reaction:

    Article Title: Pantothenate kinase 4 controls skeletal muscle substrate metabolism.
    Article Snippet: RNA from tissues was isolated by Trizol-Chloroform (peqGOLD TriFastTM, 30-2010P, VWR) extraction in homogenized tissues and precipitation with Isopropanol. .. Genomic DNAwas removed (DNAse I, EN0521, Fisher Scientific; RiboLock RNAse Inhibitor, EO0382, Fisher Scientific) and 1 μg RNA was transcribed into cDNA using LunaScript RT Super Mix (E3010L, New England Biolabs). mRNA levels were determined by qPCR using SYBR Green (Luna Universal qPCRMasterMix,M3003E,NewEnglandBiolabs) on aVIA7RealTime PCR System (4453534, Thermo Fischer Scientific). ..

    Control:

    Article Title: Genome-wide CRISPR knockout screening with viral replicons for identification of host factors involved in viral replication
    Article Snippet: .. The LunaScript RT supermix (primer-free) kit (NEB E3025S) was used to synthesize cDNA for vRNA, cRNA, and mRNA; the LunaScript RT supermix kit (NEB E3010S) was used to synthesize cDNA for the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA control. ..



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