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ls column adapter  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec ls column adapter
    Ls Column Adapter, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ls+column/LS+Column+Adapter/pmc09508471-64-0-4
    Average 94 stars, based on 14 article reviews
    ls column adapter - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Exploring the Potential Role of Interleukin-33 in Inducing Eosinophil-Mediated Fibrosis in Hepatic Alveolar Echinococcosis
    Article Snippet: 50 μL antiCD16 immunomagnetic beads (Cat. #: 130-092-010, Miltenyi biotech, Bergisch Gladbach, Germany) were added to 5 × 107 cells, mixed well, and incubated in a shaker at 4~8 °C for 30 minutes. .. After incubation, the cell antibody solution was added to the LS magnetic column (Cat. #: 130-090-544, Miltenyi biotech, Bergisch Gladbach, Germany) and the eluate was collected. ..

    Selection:

    Article Title: Low-Dose Radiotherapy Attenuates Experimental Autoimmune Arthritis by Inducing Apoptosis of Lymphocytes and Fibroblast-Like Synoviocytes.
    Article Snippet: The flow cytometry data were analyzed using FlowJo software version 10. .. B220+ B and CD4+ T cells were sorted by negative selection using MACS columns (130-090-544; Miltenyi Biotec, Bergisch Gladbach, Germany), and lysed in radioimmunoprecipitation buffer (89901; Thermo Fisher Scientific) supplemented with protease inhibitor (535142; Calbiochem, Seoul, Korea) to enable the collection of whole protein fractions. ..

    Magnetic Cell Separation:

    Article Title: Low-Dose Radiotherapy Attenuates Experimental Autoimmune Arthritis by Inducing Apoptosis of Lymphocytes and Fibroblast-Like Synoviocytes.
    Article Snippet: The flow cytometry data were analyzed using FlowJo software version 10. .. B220+ B and CD4+ T cells were sorted by negative selection using MACS columns (130-090-544; Miltenyi Biotec, Bergisch Gladbach, Germany), and lysed in radioimmunoprecipitation buffer (89901; Thermo Fisher Scientific) supplemented with protease inhibitor (535142; Calbiochem, Seoul, Korea) to enable the collection of whole protein fractions. ..

    Protease Inhibitor:

    Article Title: Low-Dose Radiotherapy Attenuates Experimental Autoimmune Arthritis by Inducing Apoptosis of Lymphocytes and Fibroblast-Like Synoviocytes.
    Article Snippet: The flow cytometry data were analyzed using FlowJo software version 10. .. B220+ B and CD4+ T cells were sorted by negative selection using MACS columns (130-090-544; Miltenyi Biotec, Bergisch Gladbach, Germany), and lysed in radioimmunoprecipitation buffer (89901; Thermo Fisher Scientific) supplemented with protease inhibitor (535142; Calbiochem, Seoul, Korea) to enable the collection of whole protein fractions. ..

    Cell Culture:

    Article Title: Protocol for sorting and culturing of mouse early erythroid progenitor BFU-E cells.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Other Pre-separation filters (30 mm) Miltenyi Biotec Cat# 130-041-407 LS columns Miltenyi Biotec Cat# 130-042-401 LS column adapter Miltenyi Biotec Cat# 130-090-544 1.5 mL tube Axygen Cat# YC-HC01008 15 mL tube Corning Cat# 430791 35 mm cell culture dish Corning Cat# CLS430165 Clean benchtop Thermo Fisher Scientific N/A FACSAria II BD Biosciences N/A 0.5% BSA PBS solution Reagent Final concentration Amount BSA 0.5% (W/V) 0.25 g 1 X PBS N/A 50 mL Total N/A 50 mL Store at 25 C for up to 1 week. .. Expansion media: Reagent Final concentration Amount StemPro-34 SFM N/A 39.5 mL L-Glutamine 2 mM 400 mL SCF (40 mg/mL) 100 ng/mL 100 mL EPO (10,000 U/mL) 0.5 U/mL 2 mL Dexamethasone (10 mM) 10 mM 4 mL 6mdA (20 mM) 100 mM 80 mL Total N/A 40 mL Store at 4 C for up to 1 month.

    Concentration Assay:

    Article Title: Protocol for sorting and culturing of mouse early erythroid progenitor BFU-E cells.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Other Pre-separation filters (30 mm) Miltenyi Biotec Cat# 130-041-407 LS columns Miltenyi Biotec Cat# 130-042-401 LS column adapter Miltenyi Biotec Cat# 130-090-544 1.5 mL tube Axygen Cat# YC-HC01008 15 mL tube Corning Cat# 430791 35 mm cell culture dish Corning Cat# CLS430165 Clean benchtop Thermo Fisher Scientific N/A FACSAria II BD Biosciences N/A 0.5% BSA PBS solution Reagent Final concentration Amount BSA 0.5% (W/V) 0.25 g 1 X PBS N/A 50 mL Total N/A 50 mL Store at 25 C for up to 1 week. .. Expansion media: Reagent Final concentration Amount StemPro-34 SFM N/A 39.5 mL L-Glutamine 2 mM 400 mL SCF (40 mg/mL) 100 ng/mL 100 mL EPO (10,000 U/mL) 0.5 U/mL 2 mL Dexamethasone (10 mM) 10 mM 4 mL 6mdA (20 mM) 100 mM 80 mL Total N/A 40 mL Store at 4 C for up to 1 month.

    other:

    Article Title: Single-cell atlas reveals characteristic changes in intrahepatic HBV-specific leukocytes
    Article Snippet: GraphPad Prism (version 8.0) GraphPad Software Inc https://www.graphpad.com/ scientificsoftware/prism/ FlowJo (version7.6.1) FlowJo, LLC https://www.flowjo.com/ solutions/flowjo Cytobank Beckman, LLC https://premium.cytobank.cn/ cytobank/experiments MATLAB R2016a N/A https://www.mathworks.com/ Normalizer (1) https://github.com/nolanlab/b ead-normalization/releases R version 3.5 R Development Core Team https://www.r-project.org/ R Studio RStudio Team, 2015 https://www.rstudio.com/ FlowSOM (2) https://github.com/SofieVG/Fl owSOM Circlize (3) https://cran.r-project.org/web/ 44 packages/circlize/index.html MCF-data-analysis (4) https://github.com/hartmannfj / MCF-data-analysis CyTOF workflow (5) https://f1000research.com/art icles/6-748# t-SNE (6) https://github.com/jkrijthe/Rts ne One-SENSE (7) N/A flowStats N/A https://www.bioconductor.org / packages/release/bioc/html/fl owStats.html pheatmap N/A https://cran.r-project.org/web/ packages/pheatmap/index.ht ml TCGAbiolinks (8) https://bioconductor.org/pack ages/release/bioc/html/TCG Abiolinks.html flowCore (9) N/A flowWorkspaceData (10) N/A Hmisc N/A https://cran.r-project.org/web/ packages/Hmisc/index.html ggplot2 N/A https://cran.r-project.org/web/ 45 packages/ggplot2/index.html



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    Miltenyi Biotec ls columns
    Ls Columns, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ls+column/LS+Columns/custom%40130-042-401%4042756835
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    ls columns - by Bioz Stars, 2026-09
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