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northernmax low/high-stringency wash buffers  (Thermo Fisher)


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    Thermo Fisher northernmax low/high-stringency wash buffers
    Northernmax Low/High Stringency Wash Buffers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/low+stringency+wash+buffer/pmc06393591-436-32-38
    Average 90 stars, based on 1 article reviews
    northernmax low/high-stringency wash buffers - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Human erythroid porphobilinogen deaminase exists in 2 splice variants.
    Article Snippet: Human porphobilinogen deaminase (PBGD) is, reportedly, encoded by 2 distinct messenger RNAs (mRNAs) transcribing from a single gene.. The ubiquitous form of the PBGD gene product is often used as an endogenous reference in gene expression studies because it is pseudogene free and has minimal transcriptional variability among tissues.. A distinct erythroid-specific gene product has also been described because of the alternate splicing of the gene.

    Article Title: Identification of the Dombrock blood group glycoprotein as a polymorphic member of the ADP-ribosyltransferase gene family
    Article Snippet: Identification of the 25 known human blood group molecules is of fundamental importance for the fields of erythroid cell biology and transfusion medicine.. Here we provide the first molecular description of the “Dombrock” blood group system.. A candidate gene was identified by in silico analyses of approximately 5000 expressed sequence tags (ESTs) from terminally differentiating human erythroid cells.

    Article Title: Identification of dispensable nucleotide sequence in 3′ untranslated region of porcine reproductive and respiratory syndrome virus
    Article Snippet: The filters were hybridized overnight, and washed with low-stringency buffers (Ambion, Austin, TX), and were incubated with alkaline phosphatase-conjugated streptavidin, followed by reaction with the chemiluminescent substrate CDP-STAR (Ambion, Austin, TX).

    Article Title: Post-transcriptional regulation of the Pseudomonas aeruginosa heme assimilation system (Has) fine-tunes extracellular heme sensing
    Article Snippet: After UV cross-linking, membranes were hybridized overnight at 68 °C in ULTRAhyb ultrasensitive hybridization buffer (ThermoFisher Scientific) with RNA-biotinylated probe 0.1 n m . The membranes were washed twice in low- and high-stringency buffers (NorthernMax low/high-stringency wash buffers, ThermoFisher Scientific), and the specific biotin-labeled RNA–RNA complex was visualized by chemiluminescent detection with the Chemiluminescent Nucleic Acid Detection Module (ThermoFisher Scientific) and Amersham Biosciences hyperfilm ECL (Amersham Biosciences).

    Article Title: Endothelial expression of CXCR7 and the regulation of systemic CXCL12 levels
    Article Snippet: After pre-hybridization, blots were incubated overnight at 68° in Ultrahyb (Ambion) containing 28 million counts/min CXCR7 riboprobe, then rinsed sequentially with low- and high-stringency buffers (Ambion) at 68°.

    Article Title: Isolation and Characterization of Dromedary Camel Coronavirus UAE-HKU23 from Dromedaries of the Middle East: Minimal Serological Cross-Reactivity between MERS Coronavirus and Dromedary Camel Coronavirus UAE-HKU23
    Article Snippet: The blot was hybridized at 42 °C overnight and washed with low- and high-stringency buffers as recommended by the manufacturer (Ambion).

    Hybridization:

    Article Title: Diversity in genomic organisation, developmental regulation and distribution of the murine PR72/B" subunits of protein phosphatase 2A
    Article Snippet: A Mouse Tissue MTN ® Blot containing poly(A + ) RNA from eight murine tissues and a Mouse Embryo MTN ® Blot containing poly(A + ) RNA from murine embryos at days 7, 11, 15 and 17 were obtained from Clontech Laboratories. .. The membranes were hybridised at 64°C using Ambion's Ultrahyb Ultrasensitive hybridisation buffer and washed with Northern Max Low and High Stringency Buffers from Ambion. .. They were exposed and analysed using ImageQuant (Molecular Dynamics).

    Article Title: A cis-acting control region is required exclusively for the tissue-specific imprinting of Gnas.
    Article Snippet: according to parental origin1.. Silencing is controlled by cisacting regulatory regions that are differentially marked during gametogenesis and can act over hundreds of kilobases to silence many genes2–6.. Two candidate imprinting control regions (ICRs) have been identified at the compact imprinted Gnas cluster on distal mouse chromosome 2, one at exon 1A upstream of Gnas itself7 and one covering the promoters for Gnasxl and the antisense Nespas (ref. 8).

    Northern Blot:

    Article Title: Diversity in genomic organisation, developmental regulation and distribution of the murine PR72/B" subunits of protein phosphatase 2A
    Article Snippet: A Mouse Tissue MTN ® Blot containing poly(A + ) RNA from eight murine tissues and a Mouse Embryo MTN ® Blot containing poly(A + ) RNA from murine embryos at days 7, 11, 15 and 17 were obtained from Clontech Laboratories. .. The membranes were hybridised at 64°C using Ambion's Ultrahyb Ultrasensitive hybridisation buffer and washed with Northern Max Low and High Stringency Buffers from Ambion. .. They were exposed and analysed using ImageQuant (Molecular Dynamics).



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    Key Resources Table

    Journal: Cell

    Article Title: Promoter of lncRNA gene PVT1 is a tumor suppressor DNA boundary element

    doi: 10.1016/j.cell.2018.03.068

    Figure Lengend Snippet: Key Resources Table

    Article Snippet: NorthernMaxTM Low Stringency Wash Buffer , Thermo Fisher , Cat#AM8673.

    Techniques: Virus, Recombinant, Ligation, Transfection, Immunoprecipitation, Bradford Assay, Reverse Transcription, Membrane, Blocking Assay, SYBR Green Assay, Sample Prep, Luciferase, Reporter Assay, Clone Assay, Northern Blot, Plasmid Preparation, Cloning, Software