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low cell chip-seq kit  (Active Motif)


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    Structured Review

    Active Motif low cell chip-seq kit
    Low Cell Chip Seq Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/low+cell+chip-seq+kit/low+cell+chip+seq+kit/pmc08050253-135-9-13
    Average 90 stars, based on 1 article reviews
    low cell chip-seq kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Multiomics analysis identifies novel facilitators of human dopaminergic neuron differentiation
    Article Snippet: To perform low-input chromatin immunoprecipitation (ChIP) for H3K27ac, the Low Cell ChIP-Seq Kit from Active Motif was used (53084).

    Article Title: The Host Adapted Fungal Pathogens of Pneumocystis Genus Utilize Genic Regional Centromeres
    Article Snippet: Chromatin preparation, immunoprecipitation and Illumina sequencing libraires were prepared using the Low Cell ChiP-Seq kit (Active Motif, Carlsbad, CA) according to the manufacturer instructions.

    Article Title: Multiomics analysis identifies novel facilitators of human dopaminergic neuron differentiation.
    Article Snippet: To perform low-input chromatin immunoprecipitation (ChIP) for H3K27ac, the Low Cell ChIP-Seq Kit from Active Motif was used (53084).

    Article Title: Regional centromere configuration in the fungal pathogens of the Pneumocystis genus.
    Article Snippet: Chromatin preparation, immunoprecipitation (IP), and Illumina sequencing libraires were prepared using the Low Cell ChIP-Seq Kit (Active Motif, Carlsbad, CA) according to the manufacturer instructions.

    Centrifugation:

    Article Title: Cytoplasmic and nuclear protein interaction networks of the synapto-nuclear messenger CRTC1 in neurons reveal cooperative chromatin binding between CREB1 and CRTC1, MEF2C and RFX3
    Article Snippet: Chromatin was fragmented using a Misonix S-3000 probe sonicator (1/8 probe tapered 419, 2:30 min, 3 sec on, 15 sec off, Power 5.5). .. Debris was removed by centrifugation at maximum speed for 10 min. Chromatin IP was performed with the Low Cell ChIP kit (Active Motif) according to the manufacturer’s instructions with modifications described below. ..

    Chromatin Immunoprecipitation:

    Article Title: Cytoplasmic and nuclear protein interaction networks of the synapto-nuclear messenger CRTC1 in neurons reveal cooperative chromatin binding between CREB1 and CRTC1, MEF2C and RFX3
    Article Snippet: Chromatin was fragmented using a Misonix S-3000 probe sonicator (1/8 probe tapered 419, 2:30 min, 3 sec on, 15 sec off, Power 5.5). .. Debris was removed by centrifugation at maximum speed for 10 min. Chromatin IP was performed with the Low Cell ChIP kit (Active Motif) according to the manufacturer’s instructions with modifications described below. ..

    Article Title: A gene regulatory network for neural induction
    Article Snippet: .. ChIP was performed by micrococcal nuclease (MNase) digestion to fragment the chromatin following a previous protocol , followed by immunoprecipitation according to the Low Cell ChIPseq kit (Active Motif, 53084) with some modifications. ..

    Article Title: Androgen receptor monomers and dimers regulate opposing biological processes in prostate cancer cells.
    Article Snippet: .. Chromatin Immunoprecipitation was then carried out using thawed chromatin corresponding to 1 million cells diluted with 200 μL in Active Motif ChIP Buffer (Active Motif #37516) and incubated with pre-clearing Protein G agarose beads prepared according to Active Motif’s Low Cell ChIP kit (Active Motif #53084). .. After pre-clearing the chromatin, agarose beads were pelleted, and cleared chromatin transferred to a new microcentrifuge tube containing 4 μg of antibody specific to the target protein (AR: Abcam ab236225).

    Article Title: Androgen receptor monomers and dimers regulate opposing biological processes in prostate cancer cells.
    Article Snippet: Next, ProteinG Agarose beads prepared the previous day according to Active Motif’s Low Cell ChIP kit were added to the antibody-bound chromatin and incubated for 4 h on a rotator at 4 °C. .. IPs were then transferred to ChIP filtration columns, washed, and eluted in 80 μL according to Active Motif’s Low Cell ChIP kit and DNA purified using SPRIselect beads at a bead:sample ratio of 1.4:1. .. After SPRI cleanup, ChIP samples and reverse crosslinked input DNA was made into sequencing Illumina libraries using Swift NGS 2S Plus (Swift Biosciences #21096) and indexed using Single Indexed Adapters withMIDs (Swift Biosciences #279384).

    Immunoprecipitation:

    Article Title: A gene regulatory network for neural induction
    Article Snippet: .. ChIP was performed by micrococcal nuclease (MNase) digestion to fragment the chromatin following a previous protocol , followed by immunoprecipitation according to the Low Cell ChIPseq kit (Active Motif, 53084) with some modifications. ..

    Incubation:

    Article Title: Androgen receptor monomers and dimers regulate opposing biological processes in prostate cancer cells.
    Article Snippet: .. Chromatin Immunoprecipitation was then carried out using thawed chromatin corresponding to 1 million cells diluted with 200 μL in Active Motif ChIP Buffer (Active Motif #37516) and incubated with pre-clearing Protein G agarose beads prepared according to Active Motif’s Low Cell ChIP kit (Active Motif #53084). .. After pre-clearing the chromatin, agarose beads were pelleted, and cleared chromatin transferred to a new microcentrifuge tube containing 4 μg of antibody specific to the target protein (AR: Abcam ab236225).

    Filtration:

    Article Title: Androgen receptor monomers and dimers regulate opposing biological processes in prostate cancer cells.
    Article Snippet: Next, ProteinG Agarose beads prepared the previous day according to Active Motif’s Low Cell ChIP kit were added to the antibody-bound chromatin and incubated for 4 h on a rotator at 4 °C. .. IPs were then transferred to ChIP filtration columns, washed, and eluted in 80 μL according to Active Motif’s Low Cell ChIP kit and DNA purified using SPRIselect beads at a bead:sample ratio of 1.4:1. .. After SPRI cleanup, ChIP samples and reverse crosslinked input DNA was made into sequencing Illumina libraries using Swift NGS 2S Plus (Swift Biosciences #21096) and indexed using Single Indexed Adapters withMIDs (Swift Biosciences #279384).

    Purification:

    Article Title: Androgen receptor monomers and dimers regulate opposing biological processes in prostate cancer cells.
    Article Snippet: Next, ProteinG Agarose beads prepared the previous day according to Active Motif’s Low Cell ChIP kit were added to the antibody-bound chromatin and incubated for 4 h on a rotator at 4 °C. .. IPs were then transferred to ChIP filtration columns, washed, and eluted in 80 μL according to Active Motif’s Low Cell ChIP kit and DNA purified using SPRIselect beads at a bead:sample ratio of 1.4:1. .. After SPRI cleanup, ChIP samples and reverse crosslinked input DNA was made into sequencing Illumina libraries using Swift NGS 2S Plus (Swift Biosciences #21096) and indexed using Single Indexed Adapters withMIDs (Swift Biosciences #279384).



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