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U.S Biomax Inc human liver tissue arrays
(A) The serum levels of alanine aminotransferase (ALT) were analyzed at 0, 3, 24, 48, and 72 h after CCl 4 administration in the acute hepatic injury model mice. Data are represented as mean ± SEM (n = 4–5 mice per group). Unpaired Student's t -test was used for statistical analysis. *** P <0.001, ** P <0.01. (B) Time-course expression of KLF6, synoviolin, Acta2 (α-SMA), and COL1A1 (collagen I) mRNA in livers inoculated with CCl 4 (black bar) or vehicle (white bar) quantified using real-time RT-PCR. Data are represented as mean ± SEM (n = 4–5 mice per group). The mRNA level was normalized relative to the amount of the transcript of 18S rRNA, a housekeeping gene. Unpaired Student's t -test was used for statistical analysis. *** P <0.001, ** P <0.01, * P <0.05. (C) Immunohistochemical analysis of <t>liver</t> sections of wt mice at 48 h after treatment with CCl 4 (n = 4) or vehicle (n = 5). The expression and localization of synoviolin (arrows, right panel) were analyzed using anti-synoviolin antibodies. Normal IgG antibody was used for the negative control (left panels). Scale bar = 100 µm. (D) Double-labeled fluorescent immunohistochemical analysis of liver sections of wt mice at 48 h after treatment with CCl 4 (n = 4). The nuclei were counterstained with DAPI (Vectashield). The expression and localization of synoviolin (green) and α-SMA (red)—a marker of activated HSCs, were analyzed using anti-synoviolin and α-SMA antibodies. Scale bar = 200 µm. (E) Double-labeled fluorescent immunohistochemical analysis of liver sections of <t>human</t> healthy liver (n = 30) and cirrhotic liver (n = 40) using human <t>tissue</t> array sections. The expression and localization of synoviolin (green label) α-SMA (red label) were analyzed using anti-synoviolin and α-SMA antibodies. Scale bar = 100 µm.
Human Liver Tissue Arrays, supplied by U.S Biomax Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/liver+tissue+array/Normal+Human+Liver/pmc02963597-85-2-9
Average 90 stars, based on 1 article reviews
human liver tissue arrays - by Bioz Stars, 2026-10
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1) Product Images from "E3 Ubiquitin Ligase Synoviolin Is Involved in Liver Fibrogenesis"

Article Title: E3 Ubiquitin Ligase Synoviolin Is Involved in Liver Fibrogenesis

Journal: PLoS ONE

doi: 10.1371/journal.pone.0013590

(A) The serum levels of alanine aminotransferase (ALT) were analyzed at 0, 3, 24, 48, and 72 h after CCl 4 administration in the acute hepatic injury model mice. Data are represented as mean ± SEM (n = 4–5 mice per group). Unpaired Student's t -test was used for statistical analysis. *** P <0.001, ** P <0.01. (B) Time-course expression of KLF6, synoviolin, Acta2 (α-SMA), and COL1A1 (collagen I) mRNA in livers inoculated with CCl 4 (black bar) or vehicle (white bar) quantified using real-time RT-PCR. Data are represented as mean ± SEM (n = 4–5 mice per group). The mRNA level was normalized relative to the amount of the transcript of 18S rRNA, a housekeeping gene. Unpaired Student's t -test was used for statistical analysis. *** P <0.001, ** P <0.01, * P <0.05. (C) Immunohistochemical analysis of liver sections of wt mice at 48 h after treatment with CCl 4 (n = 4) or vehicle (n = 5). The expression and localization of synoviolin (arrows, right panel) were analyzed using anti-synoviolin antibodies. Normal IgG antibody was used for the negative control (left panels). Scale bar = 100 µm. (D) Double-labeled fluorescent immunohistochemical analysis of liver sections of wt mice at 48 h after treatment with CCl 4 (n = 4). The nuclei were counterstained with DAPI (Vectashield). The expression and localization of synoviolin (green) and α-SMA (red)—a marker of activated HSCs, were analyzed using anti-synoviolin and α-SMA antibodies. Scale bar = 200 µm. (E) Double-labeled fluorescent immunohistochemical analysis of liver sections of human healthy liver (n = 30) and cirrhotic liver (n = 40) using human tissue array sections. The expression and localization of synoviolin (green label) α-SMA (red label) were analyzed using anti-synoviolin and α-SMA antibodies. Scale bar = 100 µm.
Figure Legend Snippet: (A) The serum levels of alanine aminotransferase (ALT) were analyzed at 0, 3, 24, 48, and 72 h after CCl 4 administration in the acute hepatic injury model mice. Data are represented as mean ± SEM (n = 4–5 mice per group). Unpaired Student's t -test was used for statistical analysis. *** P <0.001, ** P <0.01. (B) Time-course expression of KLF6, synoviolin, Acta2 (α-SMA), and COL1A1 (collagen I) mRNA in livers inoculated with CCl 4 (black bar) or vehicle (white bar) quantified using real-time RT-PCR. Data are represented as mean ± SEM (n = 4–5 mice per group). The mRNA level was normalized relative to the amount of the transcript of 18S rRNA, a housekeeping gene. Unpaired Student's t -test was used for statistical analysis. *** P <0.001, ** P <0.01, * P <0.05. (C) Immunohistochemical analysis of liver sections of wt mice at 48 h after treatment with CCl 4 (n = 4) or vehicle (n = 5). The expression and localization of synoviolin (arrows, right panel) were analyzed using anti-synoviolin antibodies. Normal IgG antibody was used for the negative control (left panels). Scale bar = 100 µm. (D) Double-labeled fluorescent immunohistochemical analysis of liver sections of wt mice at 48 h after treatment with CCl 4 (n = 4). The nuclei were counterstained with DAPI (Vectashield). The expression and localization of synoviolin (green) and α-SMA (red)—a marker of activated HSCs, were analyzed using anti-synoviolin and α-SMA antibodies. Scale bar = 200 µm. (E) Double-labeled fluorescent immunohistochemical analysis of liver sections of human healthy liver (n = 30) and cirrhotic liver (n = 40) using human tissue array sections. The expression and localization of synoviolin (green label) α-SMA (red label) were analyzed using anti-synoviolin and α-SMA antibodies. Scale bar = 100 µm.

Techniques Used: Expressing, Quantitative RT-PCR, Immunohistochemical staining, Negative Control, Labeling, Marker

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Membrane:

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Article Snippet: Reagents were purchased from Sigma (St. Louis, MO), including ATP, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and bilirubin assay kits, unless otherwise specified. .. Other reagents were SuperSignal West Pico or Femto chemiluminescent substrate and Coomassie protein assay (Pierce, Chicago, IL); protease inhibitor cocktail and polyvinylidene difluoride membrane (Roche Diagnostics, Basel, Switzerland); antibodies to EGFR, GAPDH, Akt, phospho-Akt, STAT3, phospho-STAT3, p44/42 MAPK (ERK1/2), phospho-p44/42 MAPK (ERK1/2), peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α), and cyclin D1 (Cell Signaling Technology, Danvers, MA); apoptosis-inducing factor (AIF; Santa Cruz Biotechnology, Santa Cruz, CA); PCNA, β-actin, and 5-bromo-2′-deoxyuridine (BrdU; Sigma); cytochrome c (BD Biosciences, San Diego, CA); ImmunoPure peroxidase-conjugated secondary antibodies (Pierce); and diseased human liver tissue array (LV1201; US Biomax, Rockville, MD). ..

Pyrolysis Gas Chromatography:

Article Title: Epidermal growth factor receptor restoration rescues the fatty liver regeneration in mice
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other:

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Immunohistochemistry:

Article Title: Combination Treatment with the Vimentin-Targeting Antibody hzVSF and Tenofovir Suppresses Woodchuck Hepatitis Virus Infection in Woodchucks
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Microarray:

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Image Search Results


Association between TESC gene expression and clinical prognosis. A High and low expression groups, established by dividing the TCGA-LIHC on the basis of median expression of CD133, CD90, and EpCAM. Thirteen intersecting differentially expressed genes were identified, with high expression of TESC and QSOX1 being noted in LCSCs from spheroid cultures. B TESC’s high expression in the TCGA-LIHC was significantly correlated with shorter overall survival (OS). C TESC expression in various cancers compared with that in adjacent normal tissues (if available), assessed using TIMER2.0. D RNA expression of the TESC gene in liver cancer and normal liver tissues from the ICGC database. E Protein expression of the TESC gene in liver cancer and normal liver tissues from the CPTAC database. F Immunohistochemistry (IHC) image of TESC in liver cancer and adjacent liver tissues of tissue microarray (Cat. LV1505a, TissueArray.Com). Scale bar, 60 μm. * P < 0.05; ** P < 0.01; *** P < 0.001

Journal: Clinical and Experimental Medicine

Article Title: TESC associated with poor prognosis enhances cancer stemness and migratory properties in liver cancer

doi: 10.1007/s10238-024-01469-y

Figure Lengend Snippet: Association between TESC gene expression and clinical prognosis. A High and low expression groups, established by dividing the TCGA-LIHC on the basis of median expression of CD133, CD90, and EpCAM. Thirteen intersecting differentially expressed genes were identified, with high expression of TESC and QSOX1 being noted in LCSCs from spheroid cultures. B TESC’s high expression in the TCGA-LIHC was significantly correlated with shorter overall survival (OS). C TESC expression in various cancers compared with that in adjacent normal tissues (if available), assessed using TIMER2.0. D RNA expression of the TESC gene in liver cancer and normal liver tissues from the ICGC database. E Protein expression of the TESC gene in liver cancer and normal liver tissues from the CPTAC database. F Immunohistochemistry (IHC) image of TESC in liver cancer and adjacent liver tissues of tissue microarray (Cat. LV1505a, TissueArray.Com). Scale bar, 60 μm. * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: Human liver cancer with matched cancer adjacent liver tissue array was obtained from TissueArray.Com (Cat. LV1505a, USA).

Techniques: Gene Expression, Expressing, RNA Expression, Immunohistochemistry, Microarray

(A) The serum levels of alanine aminotransferase (ALT) were analyzed at 0, 3, 24, 48, and 72 h after CCl 4 administration in the acute hepatic injury model mice. Data are represented as mean ± SEM (n = 4–5 mice per group). Unpaired Student's t -test was used for statistical analysis. *** P <0.001, ** P <0.01. (B) Time-course expression of KLF6, synoviolin, Acta2 (α-SMA), and COL1A1 (collagen I) mRNA in livers inoculated with CCl 4 (black bar) or vehicle (white bar) quantified using real-time RT-PCR. Data are represented as mean ± SEM (n = 4–5 mice per group). The mRNA level was normalized relative to the amount of the transcript of 18S rRNA, a housekeeping gene. Unpaired Student's t -test was used for statistical analysis. *** P <0.001, ** P <0.01, * P <0.05. (C) Immunohistochemical analysis of liver sections of wt mice at 48 h after treatment with CCl 4 (n = 4) or vehicle (n = 5). The expression and localization of synoviolin (arrows, right panel) were analyzed using anti-synoviolin antibodies. Normal IgG antibody was used for the negative control (left panels). Scale bar = 100 µm. (D) Double-labeled fluorescent immunohistochemical analysis of liver sections of wt mice at 48 h after treatment with CCl 4 (n = 4). The nuclei were counterstained with DAPI (Vectashield). The expression and localization of synoviolin (green) and α-SMA (red)—a marker of activated HSCs, were analyzed using anti-synoviolin and α-SMA antibodies. Scale bar = 200 µm. (E) Double-labeled fluorescent immunohistochemical analysis of liver sections of human healthy liver (n = 30) and cirrhotic liver (n = 40) using human tissue array sections. The expression and localization of synoviolin (green label) α-SMA (red label) were analyzed using anti-synoviolin and α-SMA antibodies. Scale bar = 100 µm.

Journal: PLoS ONE

Article Title: E3 Ubiquitin Ligase Synoviolin Is Involved in Liver Fibrogenesis

doi: 10.1371/journal.pone.0013590

Figure Lengend Snippet: (A) The serum levels of alanine aminotransferase (ALT) were analyzed at 0, 3, 24, 48, and 72 h after CCl 4 administration in the acute hepatic injury model mice. Data are represented as mean ± SEM (n = 4–5 mice per group). Unpaired Student's t -test was used for statistical analysis. *** P <0.001, ** P <0.01. (B) Time-course expression of KLF6, synoviolin, Acta2 (α-SMA), and COL1A1 (collagen I) mRNA in livers inoculated with CCl 4 (black bar) or vehicle (white bar) quantified using real-time RT-PCR. Data are represented as mean ± SEM (n = 4–5 mice per group). The mRNA level was normalized relative to the amount of the transcript of 18S rRNA, a housekeeping gene. Unpaired Student's t -test was used for statistical analysis. *** P <0.001, ** P <0.01, * P <0.05. (C) Immunohistochemical analysis of liver sections of wt mice at 48 h after treatment with CCl 4 (n = 4) or vehicle (n = 5). The expression and localization of synoviolin (arrows, right panel) were analyzed using anti-synoviolin antibodies. Normal IgG antibody was used for the negative control (left panels). Scale bar = 100 µm. (D) Double-labeled fluorescent immunohistochemical analysis of liver sections of wt mice at 48 h after treatment with CCl 4 (n = 4). The nuclei were counterstained with DAPI (Vectashield). The expression and localization of synoviolin (green) and α-SMA (red)—a marker of activated HSCs, were analyzed using anti-synoviolin and α-SMA antibodies. Scale bar = 200 µm. (E) Double-labeled fluorescent immunohistochemical analysis of liver sections of human healthy liver (n = 30) and cirrhotic liver (n = 40) using human tissue array sections. The expression and localization of synoviolin (green label) α-SMA (red label) were analyzed using anti-synoviolin and α-SMA antibodies. Scale bar = 100 µm.

Article Snippet: Pathologically confirmed human liver tissue arrays were obtained from U.S. Biomax (Rockville, MD).

Techniques: Expressing, Quantitative RT-PCR, Immunohistochemical staining, Negative Control, Labeling, Marker