lipofection (Mirus Bio)
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lipofection
Lipofection, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 99/100, based on 8546 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofection/TransIT+-LT1/pm41436723-381-7-9
Average 99 stars, based on 8546 article reviews
Lipofection, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 99/100, based on 8546 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofection/TransIT+-LT1/pm41436723-381-7-9
Average 99 stars, based on 8546 article reviews
lipofection - by Bioz Stars,
2026-10
99/100 stars
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Produced:Article Title: Dysregulated Retinal DPP10 Expression with Single Cell-based Next Genomic Sequencing Profiling in X-linked Juvenile Retinoschisis. Article Snippet: Please cite this article as: Cheng, H.-P., Ching, L.-J., Wang, C.-H., Mahardhika, M.R.P., Ou-Yang, H., Lee, C.-Y., Chang, Y.-L., Tsai, D.-C., Hsu, C.-C., Hwang, D.-K., Chiou, S.-H., Chen, S.-J., Lin, T.-C., Yang, Y.-P., Dysregulated Retinal DPP10 Expression with Single Cell-based Next Genomic Sequencing Profiling in X-linked Juvenile Retinoschisis, Experimental Eye Research, https://doi.org/10.1016/ j.exer.2026.111287. Mutagenesis:Article Title: Dysregulated Retinal DPP10 Expression with Single Cell-based Next Genomic Sequencing Profiling in X-linked Juvenile Retinoschisis. Article Snippet: Please cite this article as: Cheng, H.-P., Ching, L.-J., Wang, C.-H., Mahardhika, M.R.P., Ou-Yang, H., Lee, C.-Y., Chang, Y.-L., Tsai, D.-C., Hsu, C.-C., Hwang, D.-K., Chiou, S.-H., Chen, S.-J., Lin, T.-C., Yang, Y.-P., Dysregulated Retinal DPP10 Expression with Single Cell-based Next Genomic Sequencing Profiling in X-linked Juvenile Retinoschisis, Experimental Eye Research, https://doi.org/10.1016/ j.exer.2026.111287. Transfection:Article Title: Dysregulated Retinal DPP10 Expression with Single Cell-based Next Genomic Sequencing Profiling in X-linked Juvenile Retinoschisis. Article Snippet: Please cite this article as: Cheng, H.-P., Ching, L.-J., Wang, C.-H., Mahardhika, M.R.P., Ou-Yang, H., Lee, C.-Y., Chang, Y.-L., Tsai, D.-C., Hsu, C.-C., Hwang, D.-K., Chiou, S.-H., Chen, S.-J., Lin, T.-C., Yang, Y.-P., Dysregulated Retinal DPP10 Expression with Single Cell-based Next Genomic Sequencing Profiling in X-linked Juvenile Retinoschisis, Experimental Eye Research, https://doi.org/10.1016/ j.exer.2026.111287. Article Title: Chromosome 6q mediated loss of LATS1 tumour suppression as a targetable mechanism of ultra-high-risk posterior fossa A ependymoma. Article Snippet: .. Lentiviral LATS1 vector (hLATS1) and corresponding control vector (Ctl) were generated using the pLKO system, where LATS1 and control vectors are transfected into HEK293FT cells with psPAX and pMD2.G viral packaging plasmids and Article Title: Comparison of intracellular and encapsidated viral RNA reveals selective constraints on reovirus recombination Article Snippet: .. BHK-T7 cells were co-transfected with 0.8 μg of pT7 plasmids encoding each of the ten Article Title: The prenylation code regulating estrogenic activity of synthetic and natural product derivatives of resveratrol Article Snippet: 4 3xERE-Driven luciferase transcriptional activation assay: HEK-293T cells in an ~80% confluent 10-5 cm dish were suspended in 5 mL of phenol red-free DMEM (Corning, cat. 17205CV) plus 10% 6 charcoal-stripped FBS (csFBS) (ThermoFisher, cat. no. A3382101), passed through a 30-micron 7 strainer (Miltenyi Biotec, cat. no. 130-110-915), centrifuged at 600 x g for 3 minutes, and 8 resuspended in 5 mL of phenol red-free DMEM plus 10% csFBS. .. The cells were transfected in 384-9 well microplates (Greiner Bio-One CellStar, cat no. 781080) as follows: For each microplate, 1 mL 10 of cell suspension was mixed with 9.5 mL of phenol red-free DMEM plus 10% csFBS and a 11 transfection mixture prepared by incubating 300 μL of phenol red-free DMEM, 5 μg of a 3xERE-12 luciferase reporter plasmid, and 0.5 μg of human wild-type ERα or ERβ expression vector with 16.5 13 μL of Article Title: Engineered regulatory states resolve context-dependent genetics in macular degeneration Article Snippet: LentiX 293T cells were purchased from Takara Bio (#632180) and maintained in DMEM (Gibco #11965092), 10% Fetal Bovine Serum (FBS) (VWR #97068-085), 100U penicillin and 100 mg/mL streptomycin (Gibco, 15140122). .. For lentiviral packaging, LentiX cells were grown to 80% confluence and co-transfected with library vector and packaging plasmids psPAX2 and pMD2G using Article Title: Identification of regulators of aryl hydrocarbon receptor signaling in human cells by genome-scale CRISPR screening Article Snippet: All cell lines were periodically tested for Mycoplasma contamination using either the Mycoplasma PCR Detection Kit (Applied Biological Materials Inc) or the Universal Mycoplasma Detection Kit (ATCC). .. To generate lentivirus, HEK293T cells were transfected with the transfer plasmid and four packaging plasmids (for expression of VSV-G, Gag/Pol, Rev, and Tat) using Article Title: Molecular triage and isoform protection safeguard ubiquitin chain editing on DNA. Article Snippet: Antibodies Mouse monoclonal ZUP1 Our accompanying study 21 N/A Rabbit polyclonal ZUP1 Merck Cat# HPA044426; RRID: AB_10960887 Rabbit monoclonal RPA70 Abcam Cat# ab79398; RRID: AB_1603759 Rabbit polyclonal RPA70 Cell Signaling Technologies Cat# 2267; RRID: AB_2180506 Rabbit polyclonal RPA32 Bethyl Cat# A300-244A; RRID: AB_185548 Rabbit monoclonal RPA32 Cell Signaling Technology Cat# 35869; RRID: AB_2799086 Rabbit polyclonal RPA14 Novus Biologicals Cat# NBP1-87141; RRID: AB_11037634 Rabbit polyclonal pRPA2 (S4/8) Bethyl Cat# A300-245A; RRID: AB_210547 Mouse monoclonal ubiquitin Thermo Fisher Scientific Cat# 14–6078-82; RRID: AB_837154 Rabbit polyclonal ubiquitin Cell Signaling Technologies Cat# 3933; RRID: AB_2180538 Rabbit polyclonal USP11 Bethyl Cat# A301-613A; RRID: AB_1211380 Mouse monoclonal TCEAL1 Santa Cruz Biotechnology Cat# sc-393621 Rabbit polyclonal TCEAL1 Merck Cat# HPA060384; RRID: AB_2732648 Rabbit monoclonal UBR5 Cell Signaling Technologies Cat# 65344; RRID: AB_2799679 Mouse monoclonal p97/VCP Santa Cruz Biotechnology Cat# sc-57492; RRID: AB_793927 Rabbit polyclonal p97/VCP Bethyl Cat# A300-589A; RRID: AB_495512 Rabbit polyclonal p97/VCP Proteintech Cat# 10736-1-AP; RRID: AB_2214635 Rabbit polyclonal NPL4 Cell Signaling Technologies Cat# 13489; RRID: AB_2798232 Rabbit polyclonal UFD1 Cell Signaling Technologies Cat# 13789; RRID: AB_2798313 Rabbit polyclonal p47/NSFL1C Bethyl Cat# A304-565A; RRID: AB_2620760 Mouse monoclonal ATXN3 Thermo Fisher Scientific Cat# MA3082; RRID: AB_2633327 Rabbit polyclonal ATXN3 Abcam Cat# ab96316; RRID: AB_10680570 Rabbit polyclonal HUWE1 Novus Biologicals Cat# NB100-652; RRID: AB_2264587 Rabbit monoclonal BAG6 Abcam Cat# ab137076; RRID: AB_2927494 Rabbit monoclonal USP1 Cell Signaling Technologies Cat# 8033; RRID: AB_10858879 Mouse monoclonal PCNA Cell Signaling Technology Cat# 2586; RRID: AB_2160343 Rabbit polyclonal Tubulin Abcam Cat# ab6046; RRID: AB_2210370 Mouse monoclonal Vinculin Merck Cat# V9264; RRID: AB_10603627 Rat monoclonal HA Merck Cat# 11867423001; RRID: AB_390918 Mouse monoclonal GFP Merck Cat# 11814460001; RRID: AB_390913 Rabbit polyclonal GFP Abcam Cat# ab290; RRID: AB_303395 Mouse monoclonal RFP ChromoTek Cat# 6g6; RRID: AB_2631395 Mouse monoclonal Flag-HRP Merck Cat# A8592; RRID: AB_439702 Donkey anti-rabbit IgG HRP-conjugated Jackson ImmunoResearch Cat# 711–035-152; RRID: AB_10015282 Donkey anti-mouse IgG HRP-conjugated Jackson ImmunoResearch Cat# 715–035-150-JIR; RRID: AB_2340770 (Continued on next page) Cell Reports 45, 118137, October 27, 2026 17 .. Donkey anti-rat IgG HRP-conjugated Jackson ImmunoResearch Cat# 712–035-150; RRID: AB_2340638 Donkey anti-mouse Alexa Fluor 488 Thermo Fisher Scientific Cat# A2120; RRID: AB_141607 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat# A21206; RRID: AB_2535792 Donkey anti-mouse Alexa Fluor 555 Thermo Fisher Scientific Cat# A31570; RRID: AB_2536180 Donkey anti-rabbit Alexa Fluor 555 Thermo Fisher Scientific Cat# A31572; RRID: AB_162543 Donkey anti-rat Alexa Fluor 488 Thermo Fisher Scientific Cat# A21208; RRID: AB_2535794 Donkey anti-rat Alexa Fluor 555 Thermo Fisher Scientific Cat# A48270; RRID: AB_2896336 Bacterial strains NEB5-alpha New England Biolabs Cat# C2987H Rosetta 2(DE3)pLysS Merck Cat# 71401–3 One Shot Stbl3 Chemically Competent E. coli Thermo Fisher Scientific Cat# C737303 Chemicals, peptides, and recombinant proteins USP2 Bio-Techne Cat# E-504 Article Title: Personalized neoantigen-targeting cancer vaccines in ovarian cancer: a phase 1 trial. Article Snippet: Personalized neoantigen-targeting cancer vaccines in the adjuvant setting are of increasing interest across malignancies.. Here we report the results of a phase 1 clinical trial testing the feasibility and safety of personalized neoantigen-targeting cancer vaccines for high-grade epithelial ovarian cancer.. ‘NeoVax’ consisted of pools of synthetic long peptides targeting patient-specific neoantigens admixed with the immunostimulant poly-ICLC, was combined with delayed administration of anti-PD-1 antibody, and was tested in ten patients (one newly diagnosed patient (Cohort A) and nine patients at first recurrence (Cohort B)) with platinum-sensitive ovarian cancer following standard-of-care platinum doublet chemotherapy. Plasmid Preparation:Article Title: Chromosome 6q mediated loss of LATS1 tumour suppression as a targetable mechanism of ultra-high-risk posterior fossa A ependymoma. Article Snippet: .. Lentiviral LATS1 vector (hLATS1) and corresponding control vector (Ctl) were generated using the pLKO system, where LATS1 and control vectors are transfected into HEK293FT cells with psPAX and pMD2.G viral packaging plasmids and Article Title: The prenylation code regulating estrogenic activity of synthetic and natural product derivatives of resveratrol Article Snippet: 4 3xERE-Driven luciferase transcriptional activation assay: HEK-293T cells in an ~80% confluent 10-5 cm dish were suspended in 5 mL of phenol red-free DMEM (Corning, cat. 17205CV) plus 10% 6 charcoal-stripped FBS (csFBS) (ThermoFisher, cat. no. A3382101), passed through a 30-micron 7 strainer (Miltenyi Biotec, cat. no. 130-110-915), centrifuged at 600 x g for 3 minutes, and 8 resuspended in 5 mL of phenol red-free DMEM plus 10% csFBS. .. The cells were transfected in 384-9 well microplates (Greiner Bio-One CellStar, cat no. 781080) as follows: For each microplate, 1 mL 10 of cell suspension was mixed with 9.5 mL of phenol red-free DMEM plus 10% csFBS and a 11 transfection mixture prepared by incubating 300 μL of phenol red-free DMEM, 5 μg of a 3xERE-12 luciferase reporter plasmid, and 0.5 μg of human wild-type ERα or ERβ expression vector with 16.5 13 μL of Article Title: Engineered regulatory states resolve context-dependent genetics in macular degeneration Article Snippet: LentiX 293T cells were purchased from Takara Bio (#632180) and maintained in DMEM (Gibco #11965092), 10% Fetal Bovine Serum (FBS) (VWR #97068-085), 100U penicillin and 100 mg/mL streptomycin (Gibco, 15140122). .. For lentiviral packaging, LentiX cells were grown to 80% confluence and co-transfected with library vector and packaging plasmids psPAX2 and pMD2G using Article Title: Identification of regulators of aryl hydrocarbon receptor signaling in human cells by genome-scale CRISPR screening Article Snippet: All cell lines were periodically tested for Mycoplasma contamination using either the Mycoplasma PCR Detection Kit (Applied Biological Materials Inc) or the Universal Mycoplasma Detection Kit (ATCC). .. To generate lentivirus, HEK293T cells were transfected with the transfer plasmid and four packaging plasmids (for expression of VSV-G, Gag/Pol, Rev, and Tat) using Control:Article Title: Chromosome 6q mediated loss of LATS1 tumour suppression as a targetable mechanism of ultra-high-risk posterior fossa A ependymoma. Article Snippet: .. Lentiviral LATS1 vector (hLATS1) and corresponding control vector (Ctl) were generated using the pLKO system, where LATS1 and control vectors are transfected into HEK293FT cells with psPAX and pMD2.G viral packaging plasmids and Generated:Article Title: Chromosome 6q mediated loss of LATS1 tumour suppression as a targetable mechanism of ultra-high-risk posterior fossa A ependymoma. Article Snippet: .. Lentiviral LATS1 vector (hLATS1) and corresponding control vector (Ctl) were generated using the pLKO system, where LATS1 and control vectors are transfected into HEK293FT cells with psPAX and pMD2.G viral packaging plasmids and Article Title: Personalized neoantigen-targeting cancer vaccines in ovarian cancer: a phase 1 trial. Article Snippet: Personalized neoantigen-targeting cancer vaccines in the adjuvant setting are of increasing interest across malignancies.. Here we report the results of a phase 1 clinical trial testing the feasibility and safety of personalized neoantigen-targeting cancer vaccines for high-grade epithelial ovarian cancer.. ‘NeoVax’ consisted of pools of synthetic long peptides targeting patient-specific neoantigens admixed with the immunostimulant poly-ICLC, was combined with delayed administration of anti-PD-1 antibody, and was tested in ten patients (one newly diagnosed patient (Cohort A) and nine patients at first recurrence (Cohort B)) with platinum-sensitive ovarian cancer following standard-of-care platinum doublet chemotherapy. Suspension:Article Title: The prenylation code regulating estrogenic activity of synthetic and natural product derivatives of resveratrol Article Snippet: 4 3xERE-Driven luciferase transcriptional activation assay: HEK-293T cells in an ~80% confluent 10-5 cm dish were suspended in 5 mL of phenol red-free DMEM (Corning, cat. 17205CV) plus 10% 6 charcoal-stripped FBS (csFBS) (ThermoFisher, cat. no. A3382101), passed through a 30-micron 7 strainer (Miltenyi Biotec, cat. no. 130-110-915), centrifuged at 600 x g for 3 minutes, and 8 resuspended in 5 mL of phenol red-free DMEM plus 10% csFBS. .. The cells were transfected in 384-9 well microplates (Greiner Bio-One CellStar, cat no. 781080) as follows: For each microplate, 1 mL 10 of cell suspension was mixed with 9.5 mL of phenol red-free DMEM plus 10% csFBS and a 11 transfection mixture prepared by incubating 300 μL of phenol red-free DMEM, 5 μg of a 3xERE-12 luciferase reporter plasmid, and 0.5 μg of human wild-type ERα or ERβ expression vector with 16.5 13 μL of Luciferase:Article Title: The prenylation code regulating estrogenic activity of synthetic and natural product derivatives of resveratrol Article Snippet: 4 3xERE-Driven luciferase transcriptional activation assay: HEK-293T cells in an ~80% confluent 10-5 cm dish were suspended in 5 mL of phenol red-free DMEM (Corning, cat. 17205CV) plus 10% 6 charcoal-stripped FBS (csFBS) (ThermoFisher, cat. no. A3382101), passed through a 30-micron 7 strainer (Miltenyi Biotec, cat. no. 130-110-915), centrifuged at 600 x g for 3 minutes, and 8 resuspended in 5 mL of phenol red-free DMEM plus 10% csFBS. .. The cells were transfected in 384-9 well microplates (Greiner Bio-One CellStar, cat no. 781080) as follows: For each microplate, 1 mL 10 of cell suspension was mixed with 9.5 mL of phenol red-free DMEM plus 10% csFBS and a 11 transfection mixture prepared by incubating 300 μL of phenol red-free DMEM, 5 μg of a 3xERE-12 luciferase reporter plasmid, and 0.5 μg of human wild-type ERα or ERβ expression vector with 16.5 13 μL of Expressing:Article Title: The prenylation code regulating estrogenic activity of synthetic and natural product derivatives of resveratrol Article Snippet: 4 3xERE-Driven luciferase transcriptional activation assay: HEK-293T cells in an ~80% confluent 10-5 cm dish were suspended in 5 mL of phenol red-free DMEM (Corning, cat. 17205CV) plus 10% 6 charcoal-stripped FBS (csFBS) (ThermoFisher, cat. no. A3382101), passed through a 30-micron 7 strainer (Miltenyi Biotec, cat. no. 130-110-915), centrifuged at 600 x g for 3 minutes, and 8 resuspended in 5 mL of phenol red-free DMEM plus 10% csFBS. .. The cells were transfected in 384-9 well microplates (Greiner Bio-One CellStar, cat no. 781080) as follows: For each microplate, 1 mL 10 of cell suspension was mixed with 9.5 mL of phenol red-free DMEM plus 10% csFBS and a 11 transfection mixture prepared by incubating 300 μL of phenol red-free DMEM, 5 μg of a 3xERE-12 luciferase reporter plasmid, and 0.5 μg of human wild-type ERα or ERβ expression vector with 16.5 13 μL of Article Title: Identification of regulators of aryl hydrocarbon receptor signaling in human cells by genome-scale CRISPR screening Article Snippet: All cell lines were periodically tested for Mycoplasma contamination using either the Mycoplasma PCR Detection Kit (Applied Biological Materials Inc) or the Universal Mycoplasma Detection Kit (ATCC). .. To generate lentivirus, HEK293T cells were transfected with the transfer plasmid and four packaging plasmids (for expression of VSV-G, Gag/Pol, Rev, and Tat) using Recombinant:Article Title: Molecular triage and isoform protection safeguard ubiquitin chain editing on DNA. Article Snippet: Antibodies Mouse monoclonal ZUP1 Our accompanying study 21 N/A Rabbit polyclonal ZUP1 Merck Cat# HPA044426; RRID: AB_10960887 Rabbit monoclonal RPA70 Abcam Cat# ab79398; RRID: AB_1603759 Rabbit polyclonal RPA70 Cell Signaling Technologies Cat# 2267; RRID: AB_2180506 Rabbit polyclonal RPA32 Bethyl Cat# A300-244A; RRID: AB_185548 Rabbit monoclonal RPA32 Cell Signaling Technology Cat# 35869; RRID: AB_2799086 Rabbit polyclonal RPA14 Novus Biologicals Cat# NBP1-87141; RRID: AB_11037634 Rabbit polyclonal pRPA2 (S4/8) Bethyl Cat# A300-245A; RRID: AB_210547 Mouse monoclonal ubiquitin Thermo Fisher Scientific Cat# 14–6078-82; RRID: AB_837154 Rabbit polyclonal ubiquitin Cell Signaling Technologies Cat# 3933; RRID: AB_2180538 Rabbit polyclonal USP11 Bethyl Cat# A301-613A; RRID: AB_1211380 Mouse monoclonal TCEAL1 Santa Cruz Biotechnology Cat# sc-393621 Rabbit polyclonal TCEAL1 Merck Cat# HPA060384; RRID: AB_2732648 Rabbit monoclonal UBR5 Cell Signaling Technologies Cat# 65344; RRID: AB_2799679 Mouse monoclonal p97/VCP Santa Cruz Biotechnology Cat# sc-57492; RRID: AB_793927 Rabbit polyclonal p97/VCP Bethyl Cat# A300-589A; RRID: AB_495512 Rabbit polyclonal p97/VCP Proteintech Cat# 10736-1-AP; RRID: AB_2214635 Rabbit polyclonal NPL4 Cell Signaling Technologies Cat# 13489; RRID: AB_2798232 Rabbit polyclonal UFD1 Cell Signaling Technologies Cat# 13789; RRID: AB_2798313 Rabbit polyclonal p47/NSFL1C Bethyl Cat# A304-565A; RRID: AB_2620760 Mouse monoclonal ATXN3 Thermo Fisher Scientific Cat# MA3082; RRID: AB_2633327 Rabbit polyclonal ATXN3 Abcam Cat# ab96316; RRID: AB_10680570 Rabbit polyclonal HUWE1 Novus Biologicals Cat# NB100-652; RRID: AB_2264587 Rabbit monoclonal BAG6 Abcam Cat# ab137076; RRID: AB_2927494 Rabbit monoclonal USP1 Cell Signaling Technologies Cat# 8033; RRID: AB_10858879 Mouse monoclonal PCNA Cell Signaling Technology Cat# 2586; RRID: AB_2160343 Rabbit polyclonal Tubulin Abcam Cat# ab6046; RRID: AB_2210370 Mouse monoclonal Vinculin Merck Cat# V9264; RRID: AB_10603627 Rat monoclonal HA Merck Cat# 11867423001; RRID: AB_390918 Mouse monoclonal GFP Merck Cat# 11814460001; RRID: AB_390913 Rabbit polyclonal GFP Abcam Cat# ab290; RRID: AB_303395 Mouse monoclonal RFP ChromoTek Cat# 6g6; RRID: AB_2631395 Mouse monoclonal Flag-HRP Merck Cat# A8592; RRID: AB_439702 Donkey anti-rabbit IgG HRP-conjugated Jackson ImmunoResearch Cat# 711–035-152; RRID: AB_10015282 Donkey anti-mouse IgG HRP-conjugated Jackson ImmunoResearch Cat# 715–035-150-JIR; RRID: AB_2340770 (Continued on next page) Cell Reports 45, 118137, October 27, 2026 17 .. Donkey anti-rat IgG HRP-conjugated Jackson ImmunoResearch Cat# 712–035-150; RRID: AB_2340638 Donkey anti-mouse Alexa Fluor 488 Thermo Fisher Scientific Cat# A2120; RRID: AB_141607 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat# A21206; RRID: AB_2535792 Donkey anti-mouse Alexa Fluor 555 Thermo Fisher Scientific Cat# A31570; RRID: AB_2536180 Donkey anti-rabbit Alexa Fluor 555 Thermo Fisher Scientific Cat# A31572; RRID: AB_162543 Donkey anti-rat Alexa Fluor 488 Thermo Fisher Scientific Cat# A21208; RRID: AB_2535794 Donkey anti-rat Alexa Fluor 555 Thermo Fisher Scientific Cat# A48270; RRID: AB_2896336 Bacterial strains NEB5-alpha New England Biolabs Cat# C2987H Rosetta 2(DE3)pLysS Merck Cat# 71401–3 One Shot Stbl3 Chemically Competent E. coli Thermo Fisher Scientific Cat# C737303 Chemicals, peptides, and recombinant proteins USP2 Bio-Techne Cat# E-504 Ubiquitin Proteomics:Article Title: Molecular triage and isoform protection safeguard ubiquitin chain editing on DNA. Article Snippet: Antibodies Mouse monoclonal ZUP1 Our accompanying study 21 N/A Rabbit polyclonal ZUP1 Merck Cat# HPA044426; RRID: AB_10960887 Rabbit monoclonal RPA70 Abcam Cat# ab79398; RRID: AB_1603759 Rabbit polyclonal RPA70 Cell Signaling Technologies Cat# 2267; RRID: AB_2180506 Rabbit polyclonal RPA32 Bethyl Cat# A300-244A; RRID: AB_185548 Rabbit monoclonal RPA32 Cell Signaling Technology Cat# 35869; RRID: AB_2799086 Rabbit polyclonal RPA14 Novus Biologicals Cat# NBP1-87141; RRID: AB_11037634 Rabbit polyclonal pRPA2 (S4/8) Bethyl Cat# A300-245A; RRID: AB_210547 Mouse monoclonal ubiquitin Thermo Fisher Scientific Cat# 14–6078-82; RRID: AB_837154 Rabbit polyclonal ubiquitin Cell Signaling Technologies Cat# 3933; RRID: AB_2180538 Rabbit polyclonal USP11 Bethyl Cat# A301-613A; RRID: AB_1211380 Mouse monoclonal TCEAL1 Santa Cruz Biotechnology Cat# sc-393621 Rabbit polyclonal TCEAL1 Merck Cat# HPA060384; RRID: AB_2732648 Rabbit monoclonal UBR5 Cell Signaling Technologies Cat# 65344; RRID: AB_2799679 Mouse monoclonal p97/VCP Santa Cruz Biotechnology Cat# sc-57492; RRID: AB_793927 Rabbit polyclonal p97/VCP Bethyl Cat# A300-589A; RRID: AB_495512 Rabbit polyclonal p97/VCP Proteintech Cat# 10736-1-AP; RRID: AB_2214635 Rabbit polyclonal NPL4 Cell Signaling Technologies Cat# 13489; RRID: AB_2798232 Rabbit polyclonal UFD1 Cell Signaling Technologies Cat# 13789; RRID: AB_2798313 Rabbit polyclonal p47/NSFL1C Bethyl Cat# A304-565A; RRID: AB_2620760 Mouse monoclonal ATXN3 Thermo Fisher Scientific Cat# MA3082; RRID: AB_2633327 Rabbit polyclonal ATXN3 Abcam Cat# ab96316; RRID: AB_10680570 Rabbit polyclonal HUWE1 Novus Biologicals Cat# NB100-652; RRID: AB_2264587 Rabbit monoclonal BAG6 Abcam Cat# ab137076; RRID: AB_2927494 Rabbit monoclonal USP1 Cell Signaling Technologies Cat# 8033; RRID: AB_10858879 Mouse monoclonal PCNA Cell Signaling Technology Cat# 2586; RRID: AB_2160343 Rabbit polyclonal Tubulin Abcam Cat# ab6046; RRID: AB_2210370 Mouse monoclonal Vinculin Merck Cat# V9264; RRID: AB_10603627 Rat monoclonal HA Merck Cat# 11867423001; RRID: AB_390918 Mouse monoclonal GFP Merck Cat# 11814460001; RRID: AB_390913 Rabbit polyclonal GFP Abcam Cat# ab290; RRID: AB_303395 Mouse monoclonal RFP ChromoTek Cat# 6g6; RRID: AB_2631395 Mouse monoclonal Flag-HRP Merck Cat# A8592; RRID: AB_439702 Donkey anti-rabbit IgG HRP-conjugated Jackson ImmunoResearch Cat# 711–035-152; RRID: AB_10015282 Donkey anti-mouse IgG HRP-conjugated Jackson ImmunoResearch Cat# 715–035-150-JIR; RRID: AB_2340770 (Continued on next page) Cell Reports 45, 118137, October 27, 2026 17 .. Donkey anti-rat IgG HRP-conjugated Jackson ImmunoResearch Cat# 712–035-150; RRID: AB_2340638 Donkey anti-mouse Alexa Fluor 488 Thermo Fisher Scientific Cat# A2120; RRID: AB_141607 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat# A21206; RRID: AB_2535792 Donkey anti-mouse Alexa Fluor 555 Thermo Fisher Scientific Cat# A31570; RRID: AB_2536180 Donkey anti-rabbit Alexa Fluor 555 Thermo Fisher Scientific Cat# A31572; RRID: AB_162543 Donkey anti-rat Alexa Fluor 488 Thermo Fisher Scientific Cat# A21208; RRID: AB_2535794 Donkey anti-rat Alexa Fluor 555 Thermo Fisher Scientific Cat# A48270; RRID: AB_2896336 Bacterial strains NEB5-alpha New England Biolabs Cat# C2987H Rosetta 2(DE3)pLysS Merck Cat# 71401–3 One Shot Stbl3 Chemically Competent E. coli Thermo Fisher Scientific Cat# C737303 Chemicals, peptides, and recombinant proteins USP2 Bio-Techne Cat# E-504 Protease Inhibitor:Article Title: Molecular triage and isoform protection safeguard ubiquitin chain editing on DNA. Article Snippet: Antibodies Mouse monoclonal ZUP1 Our accompanying study 21 N/A Rabbit polyclonal ZUP1 Merck Cat# HPA044426; RRID: AB_10960887 Rabbit monoclonal RPA70 Abcam Cat# ab79398; RRID: AB_1603759 Rabbit polyclonal RPA70 Cell Signaling Technologies Cat# 2267; RRID: AB_2180506 Rabbit polyclonal RPA32 Bethyl Cat# A300-244A; RRID: AB_185548 Rabbit monoclonal RPA32 Cell Signaling Technology Cat# 35869; RRID: AB_2799086 Rabbit polyclonal RPA14 Novus Biologicals Cat# NBP1-87141; RRID: AB_11037634 Rabbit polyclonal pRPA2 (S4/8) Bethyl Cat# A300-245A; RRID: AB_210547 Mouse monoclonal ubiquitin Thermo Fisher Scientific Cat# 14–6078-82; RRID: AB_837154 Rabbit polyclonal ubiquitin Cell Signaling Technologies Cat# 3933; RRID: AB_2180538 Rabbit polyclonal USP11 Bethyl Cat# A301-613A; RRID: AB_1211380 Mouse monoclonal TCEAL1 Santa Cruz Biotechnology Cat# sc-393621 Rabbit polyclonal TCEAL1 Merck Cat# HPA060384; RRID: AB_2732648 Rabbit monoclonal UBR5 Cell Signaling Technologies Cat# 65344; RRID: AB_2799679 Mouse monoclonal p97/VCP Santa Cruz Biotechnology Cat# sc-57492; RRID: AB_793927 Rabbit polyclonal p97/VCP Bethyl Cat# A300-589A; RRID: AB_495512 Rabbit polyclonal p97/VCP Proteintech Cat# 10736-1-AP; RRID: AB_2214635 Rabbit polyclonal NPL4 Cell Signaling Technologies Cat# 13489; RRID: AB_2798232 Rabbit polyclonal UFD1 Cell Signaling Technologies Cat# 13789; RRID: AB_2798313 Rabbit polyclonal p47/NSFL1C Bethyl Cat# A304-565A; RRID: AB_2620760 Mouse monoclonal ATXN3 Thermo Fisher Scientific Cat# MA3082; RRID: AB_2633327 Rabbit polyclonal ATXN3 Abcam Cat# ab96316; RRID: AB_10680570 Rabbit polyclonal HUWE1 Novus Biologicals Cat# NB100-652; RRID: AB_2264587 Rabbit monoclonal BAG6 Abcam Cat# ab137076; RRID: AB_2927494 Rabbit monoclonal USP1 Cell Signaling Technologies Cat# 8033; RRID: AB_10858879 Mouse monoclonal PCNA Cell Signaling Technology Cat# 2586; RRID: AB_2160343 Rabbit polyclonal Tubulin Abcam Cat# ab6046; RRID: AB_2210370 Mouse monoclonal Vinculin Merck Cat# V9264; RRID: AB_10603627 Rat monoclonal HA Merck Cat# 11867423001; RRID: AB_390918 Mouse monoclonal GFP Merck Cat# 11814460001; RRID: AB_390913 Rabbit polyclonal GFP Abcam Cat# ab290; RRID: AB_303395 Mouse monoclonal RFP ChromoTek Cat# 6g6; RRID: AB_2631395 Mouse monoclonal Flag-HRP Merck Cat# A8592; RRID: AB_439702 Donkey anti-rabbit IgG HRP-conjugated Jackson ImmunoResearch Cat# 711–035-152; RRID: AB_10015282 Donkey anti-mouse IgG HRP-conjugated Jackson ImmunoResearch Cat# 715–035-150-JIR; RRID: AB_2340770 (Continued on next page) Cell Reports 45, 118137, October 27, 2026 17 .. Donkey anti-rat IgG HRP-conjugated Jackson ImmunoResearch Cat# 712–035-150; RRID: AB_2340638 Donkey anti-mouse Alexa Fluor 488 Thermo Fisher Scientific Cat# A2120; RRID: AB_141607 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat# A21206; RRID: AB_2535792 Donkey anti-mouse Alexa Fluor 555 Thermo Fisher Scientific Cat# A31570; RRID: AB_2536180 Donkey anti-rabbit Alexa Fluor 555 Thermo Fisher Scientific Cat# A31572; RRID: AB_162543 Donkey anti-rat Alexa Fluor 488 Thermo Fisher Scientific Cat# A21208; RRID: AB_2535794 Donkey anti-rat Alexa Fluor 555 Thermo Fisher Scientific Cat# A48270; RRID: AB_2896336 Bacterial strains NEB5-alpha New England Biolabs Cat# C2987H Rosetta 2(DE3)pLysS Merck Cat# 71401–3 One Shot Stbl3 Chemically Competent E. coli Thermo Fisher Scientific Cat# C737303 Chemicals, peptides, and recombinant proteins USP2 Bio-Techne Cat# E-504 96 Well Plates:Article Title: Personalized neoantigen-targeting cancer vaccines in ovarian cancer: a phase 1 trial. Article Snippet: Personalized neoantigen-targeting cancer vaccines in the adjuvant setting are of increasing interest across malignancies.. Here we report the results of a phase 1 clinical trial testing the feasibility and safety of personalized neoantigen-targeting cancer vaccines for high-grade epithelial ovarian cancer.. ‘NeoVax’ consisted of pools of synthetic long peptides targeting patient-specific neoantigens admixed with the immunostimulant poly-ICLC, was combined with delayed administration of anti-PD-1 antibody, and was tested in ten patients (one newly diagnosed patient (Cohort A) and nine patients at first recurrence (Cohort B)) with platinum-sensitive ovarian cancer following standard-of-care platinum doublet chemotherapy. |