metamorph linescan function (MetaMorph Inc)

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MetaMorph Inc metamorph linescan function

GFP-calcium-calmodulin-dependent protein kinase (CaMKII) expression and translocation after stimulation with 100 μM glutamate with 10 μM glycine via pipette. (A) GFP-CaMKII expression prior to stimulation. (B) DIC image of the same neuron with measured dendrites highlighted in red (right) and blue (left) and an arrow indicating the position of the micropipette. Scale bar = 50 μm. (C) Time-lapse subtraction images (current fluorescence level minus initial fluorescence level; increases in fluorescence appear whiter while decreases are darker/black). Hollow white arrows indicate dendrite locations that show increased relative fluorescence over time, solid white arrow indicates the location where relative fluorescence level decreases. (D) <t>Linescan</t> measures of the same neuron shown in (C) indicating fluorescence levels for respective dendrites, red (top) and blue (bottom), starting proximal to the cell body. Brackets denote regions that correspond to boxes shown in (A,B) . Black linescan trace indicates fluorescence levels measured at first post-stimulation image capture for comparison. Arrowhead indicates the location of the stimulation pipette.

Metamorph Linescan Function, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/linescan+function/linescan+module+of+metamorph+software/pmc07019030-56-24-23
Average 90 stars, based on 1 article reviews

metamorph linescan function - by Bioz Stars, 2026-09

90/100 stars

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1) Product Images from "Locally-Induced CaMKII Translocation Requires Nucleotide Binding"

Article Title: Locally-Induced CaMKII Translocation Requires Nucleotide Binding

Journal: Frontiers in Synaptic Neuroscience

doi: 10.3389/fnsyn.2020.00004

Figure Legend Snippet: GFP-calcium-calmodulin-dependent protein kinase (CaMKII) expression and translocation after stimulation with 100 μM glutamate with 10 μM glycine via pipette. (A) GFP-CaMKII expression prior to stimulation. (B) DIC image of the same neuron with measured dendrites highlighted in red (right) and blue (left) and an arrow indicating the position of the micropipette. Scale bar = 50 μm. (C) Time-lapse subtraction images (current fluorescence level minus initial fluorescence level; increases in fluorescence appear whiter while decreases are darker/black). Hollow white arrows indicate dendrite locations that show increased relative fluorescence over time, solid white arrow indicates the location where relative fluorescence level decreases. (D) Linescan measures of the same neuron shown in (C) indicating fluorescence levels for respective dendrites, red (top) and blue (bottom), starting proximal to the cell body. Brackets denote regions that correspond to boxes shown in (A,B) . Black linescan trace indicates fluorescence levels measured at first post-stimulation image capture for comparison. Arrowhead indicates the location of the stimulation pipette.

Techniques Used: Expressing, Translocation Assay, Transferring, Fluorescence

Control:

Article Title: Septin filament compaction into rings requires the anillin Mid2 and contractile ring constriction.
Article Snippet: .. To compare thewidth of Spn1-GFP andMid2-ENVY at the two different time points indicated in the text in control andmutant cells, the linescan tool (MetaMorph software 7.7.8) was oriented parallel to the long axis of the cell. ..

Microscopy:

Article Title: UNC-2 CaV2 Channel Localization at Presynaptic Active Zones Depends on UNC-10/RIM and SYD-2/Liprin-α in<i>Caenorhabditis elegans</i>
Article Snippet: .. A line-scanning method in MetaMorph Microscopy Automation and Image Analysis Software (RRID:SCR_ 002368) was used for quantifying the average fluorescence intensity. ..

Software:

Fluorescence:

Article Title: Hidden proteome of synaptic vesicles in the mammalian brain
Article Snippet: .. For quantifying TagRFP and SypHy fluorescence, line scan function in MetaMorph software was used. ..

other:

Article Title: Pch2 orchestrates the meiotic recombination checkpoint from the cytoplasm
Article Snippet: The line-scan tool of the MetaMorph software was used to measure and plot the fluorescence intensity profile across the cell in .

Article Title: Characterization of Pch2 localization determinants reveals a nucleolar-independent role in the meiotic recombination checkpoint.
Article Snippet: The line-scan tool of the MetaMorph software was used to measure and plot the fluorescence intensity profile across the cytoplasm and nucleus/nucleolus.

Article Title: Klp2 and Ase1 synergize to maintain meiotic spindle stability during metaphase I
Article Snippet: Ase1-GFP signal intensity was measured using the Linescan function in MetaMorph 7.7.

Article Title: Asymmetry is defined during meiosis in the oocyte of the parthenogenetic nematode Diploscapter pachys.
Article Snippet: Linescans were obtained in Metamorph using a 3 μm line width in average mode drawn along the AP axis of the embryo, and background was subtracted.

Article Title: C. elegans Clarinet/CLA-1 recruits RIMB-1/RIM-binding protein and UNC-13 to orchestrate presynaptic neurotransmitter release
Article Snippet: With the exception of UNC-13 data, a line-scanning method (Metamorph) was utilized to quantify the maximal projection images to produce the average peak fluorescent intensity. (Details in SI Appendix , Methods ).

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