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linescan function  (MetaMorph Inc)

 
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    MetaMorph Inc linescan function
    Linescan Function, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/linescan+function+of/linescan+function/pm38201668-100-13-15
    Average 90 stars, based on 1 article reviews
    linescan function - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Diffusion-based Assay:

    Article Title: Facilitated Transport across Glycocalyceal Barriers in the Chick Chorioallantoic Membrane.
    Article Snippet: .. To assess tracer diffusion from the original application site, we used the standard linescan function (MetaMorph) to measure color intensity values along a linear (35 mm × 6 mm) region of interest. ..

    other:

    Article Title: α 2 δ-4 and Cachd1 Proteins Are Regulators of Presynaptic Functions.
    Article Snippet: Colocalization of Synaptic Proteins To determine the synaptic localization of HA-tagged α2δ subunits and Cachd1, as well as of presynaptic (synapsin, vGAT, vGLUT1, CaV2.1) and postsynaptic proteins (GABAAR subtypes, gephyrin, GLUR1), the “line scan” function was applied in MetaMorph (Molec- Int.

    Article Title: Dynein and muskelin control myosin VI delivery towards the neuronal nucleus
    Article Snippet: Intensity profiles along dendrites were created with the “line scan” function and correlation scatter plots as well as calculation of Pearson ́s correlation coefficient was performed with MetaMorph “Correlation Plot” function.

    Article Title: Multi-site fungicides suppress banana Panama disease, caused by Fusarium oxysporum f. sp. cubense Tropical Race 4.
    Article Snippet: Intensity profiles TMRM fluorescence in germ tubes was done using the "line-scan" function in MetaMorph.

    Article Title: New insights into the functions of ACBD4/5-like proteins using a combined phylogenetic and experimental approach across model organisms.
    Article Snippet: To measure peroxisome distribution (GFP-SKL) in hyphal cells, the mean fluorescent intensity over the length of individual cells was measured using the line-scan function in MetaMorph.

    Fluorescence:

    Article Title: C-type Natriuretic Peptide-induced PKA Activation Promotes Endochondral Bone Formation in Hypertrophic Chondrocytes.
    Article Snippet: .. Fluorescence intensity along the long axis of the proximal growth plate was acquired using the line scan function in MetaMorph. ..



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    GFP-calcium-calmodulin-dependent protein kinase (CaMKII) expression and translocation after stimulation with 100 μM glutamate with 10 μM glycine via pipette. (A) GFP-CaMKII expression prior to stimulation. (B) DIC image of the same neuron with measured dendrites highlighted in red (right) and blue (left) and an arrow indicating the position of the micropipette. Scale bar = 50 μm. (C) Time-lapse subtraction images (current fluorescence level minus initial fluorescence level; increases in fluorescence appear whiter while decreases are darker/black). Hollow white arrows indicate dendrite locations that show increased relative fluorescence over time, solid white arrow indicates the location where relative fluorescence level decreases. (D) <t>Linescan</t> measures of the same neuron shown in (C) indicating fluorescence levels for respective dendrites, red (top) and blue (bottom), starting proximal to the cell body. Brackets denote regions that correspond to boxes shown in (A,B) . Black linescan trace indicates fluorescence levels measured at first post-stimulation image capture for comparison. Arrowhead indicates the location of the stimulation pipette.
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    GFP-calcium-calmodulin-dependent protein kinase (CaMKII) expression and translocation after stimulation with 100 μM glutamate with 10 μM glycine via pipette. (A) GFP-CaMKII expression prior to stimulation. (B) DIC image of the same neuron with measured dendrites highlighted in red (right) and blue (left) and an arrow indicating the position of the micropipette. Scale bar = 50 μm. (C) Time-lapse subtraction images (current fluorescence level minus initial fluorescence level; increases in fluorescence appear whiter while decreases are darker/black). Hollow white arrows indicate dendrite locations that show increased relative fluorescence over time, solid white arrow indicates the location where relative fluorescence level decreases. (D) <t>Linescan</t> measures of the same neuron shown in (C) indicating fluorescence levels for respective dendrites, red (top) and blue (bottom), starting proximal to the cell body. Brackets denote regions that correspond to boxes shown in (A,B) . Black linescan trace indicates fluorescence levels measured at first post-stimulation image capture for comparison. Arrowhead indicates the location of the stimulation pipette.
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    Image Search Results


    GFP-calcium-calmodulin-dependent protein kinase (CaMKII) expression and translocation after stimulation with 100 μM glutamate with 10 μM glycine via pipette. (A) GFP-CaMKII expression prior to stimulation. (B) DIC image of the same neuron with measured dendrites highlighted in red (right) and blue (left) and an arrow indicating the position of the micropipette. Scale bar = 50 μm. (C) Time-lapse subtraction images (current fluorescence level minus initial fluorescence level; increases in fluorescence appear whiter while decreases are darker/black). Hollow white arrows indicate dendrite locations that show increased relative fluorescence over time, solid white arrow indicates the location where relative fluorescence level decreases. (D) Linescan measures of the same neuron shown in (C) indicating fluorescence levels for respective dendrites, red (top) and blue (bottom), starting proximal to the cell body. Brackets denote regions that correspond to boxes shown in (A,B) . Black linescan trace indicates fluorescence levels measured at first post-stimulation image capture for comparison. Arrowhead indicates the location of the stimulation pipette.

    Journal: Frontiers in Synaptic Neuroscience

    Article Title: Locally-Induced CaMKII Translocation Requires Nucleotide Binding

    doi: 10.3389/fnsyn.2020.00004

    Figure Lengend Snippet: GFP-calcium-calmodulin-dependent protein kinase (CaMKII) expression and translocation after stimulation with 100 μM glutamate with 10 μM glycine via pipette. (A) GFP-CaMKII expression prior to stimulation. (B) DIC image of the same neuron with measured dendrites highlighted in red (right) and blue (left) and an arrow indicating the position of the micropipette. Scale bar = 50 μm. (C) Time-lapse subtraction images (current fluorescence level minus initial fluorescence level; increases in fluorescence appear whiter while decreases are darker/black). Hollow white arrows indicate dendrite locations that show increased relative fluorescence over time, solid white arrow indicates the location where relative fluorescence level decreases. (D) Linescan measures of the same neuron shown in (C) indicating fluorescence levels for respective dendrites, red (top) and blue (bottom), starting proximal to the cell body. Brackets denote regions that correspond to boxes shown in (A,B) . Black linescan trace indicates fluorescence levels measured at first post-stimulation image capture for comparison. Arrowhead indicates the location of the stimulation pipette.

    Article Snippet: The density of GFP-CaMKII puncta was calculated by dividing the number of identified puncta by the length of the dendrite measured using the MetaMorph linescan function yielding a value in puncta/μm.

    Techniques: Expressing, Translocation Assay, Transferring, Fluorescence