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the free on-line pcr array data analysis software  (Qiagen)


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    Structured Review

    Qiagen the free on-line pcr array data analysis software
    The Free On Line Pcr Array Data Analysis Software, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/line+data+analysis/rt2+profiler+pcr+array/pmc04698038-149-8-16
    Average 90 stars, based on 1 article reviews
    the free on-line pcr array data analysis software - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Evidence for a JAK2/STAT3 proinflammatory and vasculogenic mimicry interrelated molecular signature in adipocyte-derived mesenchymal stromal/stem cells
    Article Snippet: .. Premade RT 2 Profiler PCR arrays for Human Inflammatory Cytokines and Receptors (PAHS-011ZD) were purchased from QIAGEN and used following the manufacturer’s instructions. .. Briefly, the genomic DNA was removed before 0.5 μg of total RNA was reverse transcribed via the RT 2 First Strand Kit (QIAGEN, 330404).

    Article Title: Long non-coding RNA CHROMR regulates antiviral immunity in humans
    Article Snippet: .. RNA was isolated from THP-1 macrophages stable overexpressing CHROMR or an empty vector control, reverse-transcribed, and qPCR analysis of Type I Interferon Response genes was performed using RT2 Profiler PCR Arrays (Qiagen, PAHS-016ZA) according to manufacturer's protocol. ..

    Article Title: Evidence for a JAK2/STAT3 proinflammatory and vasculogenic mimicry interrelated molecular signature in adipocyte-derived mesenchymal stromal/stem cells.
    Article Snippet: .. Premade RT2 Profiler PCR arrays for Human Inflammatory Cytokines and Receptors (PAHS-011ZD) were purchased from QIAGEN and used following the manufacturer’s instructions. .. Briefly, the genomic DNA was removed before 0.5 μg of total RNA was reverse transcribed via the RT2 First Strand Kit (QIAGEN, 330404).

    Article Title: Mouse Model of Sutured Endothelial Keratoplasty Technique.
    Article Snippet: Following mRNA extraction, cDNA was prepared using the RT2 First Strand Kit (QIAGEN, Hilden, Germany). .. The PCR array method was used for the simultaneous analysis of multiple gene expressions using RT2 Profiler PCR Arrays (QIAGEN). .. The RT2 Profiler PCR Array for Mouse Innate & Adaptive Immune Responses (PAMM-052ZA, QIAGEN) was used for this experiment.

    Article Title:
    Article Snippet: .. Collagen I (2D model) IPF CDM (3D model)a b Supplementary Fig.6 | Gene expression of IPF KRT5+ cells compared to healthy KRT5+ cells cultured on collagen I or IPF CDMs. a,b Volcano plots showing gene expression changes determined by Qiagen RT2 PCR Array between KRT5+ cells from IPF patients (n = 3) vs. healthy controls (n = 3) cultured on a collagen I or b IPF CDMs. ..

    Article Title: Metabolic reprogramming during ineffective erythropoiesis in β-thalassemia/HbE disease.
    Article Snippet: The cDNA was mixed with RT2 SYBR Green qPCR Mastermix (QIAGEN, cat. No. 330500). .. The metabolic gene expression profiles were determined using an RT2 Profiler PCR array with a 96-well plate format coated with specific primers and probes of 84 glucose metabolic genes (Qiagen). .. The PCR array plate was subjected to a real-time PCR machine (Biorad, CFX 96).

    Article Title:
    Article Snippet: 2 μl of DNA were mixed with 12,5 μl of TaqMan Genotyping Master Mix (Applied Biosystem) and 0.6 μl of the SNP Genotyping Assay (SNP ID: rs34631763, Thermo Fisher Scientific). .. To test the gene expression of 84 genes involved in DNA damage signaling pathways, the RT2 ProfilerTM PCR Array (Mouse DNA Damage Signaling Pathway from Qiagen) was used and performed as described in the manufacturer's protocol. .. To test the gene expression of 84 genes involved in DNA damage signaling pathways, the RT2 Profiler PCR Array (Mouse DNA Damage Signaling Pathway from Qiagen) was used and performed as described in the manufacturer's protocol.

    Article Title: The potassium channel K 2P 2.1 shapes the morphology and function of brain endothelial cells via actin network remodeling
    Article Snippet: .. For analyzing the transcriptome with RT 2 Profiler PCR array (Qiagen), RT 2 First Stand Kit (Qiagen) was used for cDNA synthesis. .. For analysis of transcripts related to cytoskeletal regulation, cDNA was prepared for quantitative real time PCR (qRT-PCR) using the RT 2 SYBR Green qPCR Mastermix (Qiagen) and loaded onto plates from the RT 2 Profiler PCR Array Mouse Cytoskeleton Regulators (Qiagen, PAMM-088Z).

    Isolation:

    Article Title: Long non-coding RNA CHROMR regulates antiviral immunity in humans
    Article Snippet: .. RNA was isolated from THP-1 macrophages stable overexpressing CHROMR or an empty vector control, reverse-transcribed, and qPCR analysis of Type I Interferon Response genes was performed using RT2 Profiler PCR Arrays (Qiagen, PAHS-016ZA) according to manufacturer's protocol. ..

    Plasmid Preparation:

    Article Title: Long non-coding RNA CHROMR regulates antiviral immunity in humans
    Article Snippet: .. RNA was isolated from THP-1 macrophages stable overexpressing CHROMR or an empty vector control, reverse-transcribed, and qPCR analysis of Type I Interferon Response genes was performed using RT2 Profiler PCR Arrays (Qiagen, PAHS-016ZA) according to manufacturer's protocol. ..

    Control:

    Article Title: Long non-coding RNA CHROMR regulates antiviral immunity in humans
    Article Snippet: .. RNA was isolated from THP-1 macrophages stable overexpressing CHROMR or an empty vector control, reverse-transcribed, and qPCR analysis of Type I Interferon Response genes was performed using RT2 Profiler PCR Arrays (Qiagen, PAHS-016ZA) according to manufacturer's protocol. ..

    Reverse Transcription:

    Article Title: Long non-coding RNA CHROMR regulates antiviral immunity in humans
    Article Snippet: .. RNA was isolated from THP-1 macrophages stable overexpressing CHROMR or an empty vector control, reverse-transcribed, and qPCR analysis of Type I Interferon Response genes was performed using RT2 Profiler PCR Arrays (Qiagen, PAHS-016ZA) according to manufacturer's protocol. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Long non-coding RNA CHROMR regulates antiviral immunity in humans
    Article Snippet: .. RNA was isolated from THP-1 macrophages stable overexpressing CHROMR or an empty vector control, reverse-transcribed, and qPCR analysis of Type I Interferon Response genes was performed using RT2 Profiler PCR Arrays (Qiagen, PAHS-016ZA) according to manufacturer's protocol. ..

    Analysis:

    Article Title: Long non-coding RNA CHROMR regulates antiviral immunity in humans
    Article Snippet: .. RNA was isolated from THP-1 macrophages stable overexpressing CHROMR or an empty vector control, reverse-transcribed, and qPCR analysis of Type I Interferon Response genes was performed using RT2 Profiler PCR Arrays (Qiagen, PAHS-016ZA) according to manufacturer's protocol. ..

    Gene Expression:

    Article Title:
    Article Snippet: .. Collagen I (2D model) IPF CDM (3D model)a b Supplementary Fig.6 | Gene expression of IPF KRT5+ cells compared to healthy KRT5+ cells cultured on collagen I or IPF CDMs. a,b Volcano plots showing gene expression changes determined by Qiagen RT2 PCR Array between KRT5+ cells from IPF patients (n = 3) vs. healthy controls (n = 3) cultured on a collagen I or b IPF CDMs. ..

    Article Title: Metabolic reprogramming during ineffective erythropoiesis in β-thalassemia/HbE disease.
    Article Snippet: The cDNA was mixed with RT2 SYBR Green qPCR Mastermix (QIAGEN, cat. No. 330500). .. The metabolic gene expression profiles were determined using an RT2 Profiler PCR array with a 96-well plate format coated with specific primers and probes of 84 glucose metabolic genes (Qiagen). .. The PCR array plate was subjected to a real-time PCR machine (Biorad, CFX 96).

    Article Title:
    Article Snippet: 2 μl of DNA were mixed with 12,5 μl of TaqMan Genotyping Master Mix (Applied Biosystem) and 0.6 μl of the SNP Genotyping Assay (SNP ID: rs34631763, Thermo Fisher Scientific). .. To test the gene expression of 84 genes involved in DNA damage signaling pathways, the RT2 ProfilerTM PCR Array (Mouse DNA Damage Signaling Pathway from Qiagen) was used and performed as described in the manufacturer's protocol. .. To test the gene expression of 84 genes involved in DNA damage signaling pathways, the RT2 Profiler PCR Array (Mouse DNA Damage Signaling Pathway from Qiagen) was used and performed as described in the manufacturer's protocol.

    Cell Culture:

    Article Title:
    Article Snippet: .. Collagen I (2D model) IPF CDM (3D model)a b Supplementary Fig.6 | Gene expression of IPF KRT5+ cells compared to healthy KRT5+ cells cultured on collagen I or IPF CDMs. a,b Volcano plots showing gene expression changes determined by Qiagen RT2 PCR Array between KRT5+ cells from IPF patients (n = 3) vs. healthy controls (n = 3) cultured on a collagen I or b IPF CDMs. ..

    Protein-Protein interactions:

    Article Title:
    Article Snippet: 2 μl of DNA were mixed with 12,5 μl of TaqMan Genotyping Master Mix (Applied Biosystem) and 0.6 μl of the SNP Genotyping Assay (SNP ID: rs34631763, Thermo Fisher Scientific). .. To test the gene expression of 84 genes involved in DNA damage signaling pathways, the RT2 ProfilerTM PCR Array (Mouse DNA Damage Signaling Pathway from Qiagen) was used and performed as described in the manufacturer's protocol. .. To test the gene expression of 84 genes involved in DNA damage signaling pathways, the RT2 Profiler PCR Array (Mouse DNA Damage Signaling Pathway from Qiagen) was used and performed as described in the manufacturer's protocol.

    cDNA Synthesis:

    Article Title: The potassium channel K 2P 2.1 shapes the morphology and function of brain endothelial cells via actin network remodeling
    Article Snippet: .. For analyzing the transcriptome with RT 2 Profiler PCR array (Qiagen), RT 2 First Stand Kit (Qiagen) was used for cDNA synthesis. .. For analysis of transcripts related to cytoskeletal regulation, cDNA was prepared for quantitative real time PCR (qRT-PCR) using the RT 2 SYBR Green qPCR Mastermix (Qiagen) and loaded onto plates from the RT 2 Profiler PCR Array Mouse Cytoskeleton Regulators (Qiagen, PAMM-088Z).



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    Fig. 2. Unsupervised hierarchical cluster analysis. The expression value (log2) for each detectable miRNA (Cq < 37) was used to determine the clustering of the samples. Red: primary OS samples; yellow: OS cell lines; blue: OB cell lines.

    Journal: Molecular oncology

    Article Title: miRNA profiling identifies deregulated miRNAs associated with osteosarcoma development and time to metastasis in two large cohorts.

    doi: 10.1002/1878-0261.12154

    Figure Lengend Snippet: Fig. 2. Unsupervised hierarchical cluster analysis. The expression value (log2) for each detectable miRNA (Cq < 37) was used to determine the clustering of the samples. Red: primary OS samples; yellow: OS cell lines; blue: OB cell lines.

    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Discovery Cohort 23 OS samples (Fresh frozen) Normal control: 3 OB cell lines Validation Cohort 78 OS samples (FFPE) Normal control: 3 OB cell lines Cohorts RNA extraction cDNA synthesis Quality control miRNA analysis Data analysis miRNeasy Mini Kit (Qiagen) miRNAeasy FFPE Kit (Qiagen) 50 ng RNA Universal cDNA Synthesis Kit II (Exiqon) 65 ng RNA Universal cDNA Synthesis Kit II (Exiqon) QC of cDNA Expression of: miR-103; miR-191-5p; miR-423 (Cq<32) UniSp6 (Spike-in) (Cq:16-18) QC of cDNA Expression of: miR-191-5p; miR-423-5p (Cq<30); UniSP4, UniSp5, UniSp6 (Spike-ins) 752 miRNAs analysed microRNA Ready-to-Use PCR, Human Panel I+II v2 (Exiqon) cDNA diluted 1:100 33 miRNAs analysed Pick&Mix microRNA PCR Panels (Exiqon) cDNA diluted 1:20 Analysis in GenEx Software (MultiD) Normalization: Mean global expression Analysis in GenEx Software (MultiD) Normalization: 5 reference miRs 78 miRs diff. expressed (OS samples vs OB cell lines) 5 reference miRs identified 29 miRs diff. expressed (OS samples vs OB cell lines) 33 miRs diff. expressed Exclusion of miRs with no expr. diff. btw.

    Techniques: Expressing

    Fig. 4. Network of validated target genes. Network of validated target genes associated with the terms ‘Cell Death and Survival, Cancer, Hematological System Development and Function’. The relation of 29 validated target genes (miRWalk2.0) assembled in a network associated with 11 miRNAs altered in OS in this study. Eight genes were downregulated and 21 upregulated. Red: downregulated; blue: upregulated. Gray lines: association between genes; Red lines: association between gene and miRNA; continuous line: direct association; dashed line: indirect association.

    Journal: Molecular oncology

    Article Title: miRNA profiling identifies deregulated miRNAs associated with osteosarcoma development and time to metastasis in two large cohorts.

    doi: 10.1002/1878-0261.12154

    Figure Lengend Snippet: Fig. 4. Network of validated target genes. Network of validated target genes associated with the terms ‘Cell Death and Survival, Cancer, Hematological System Development and Function’. The relation of 29 validated target genes (miRWalk2.0) assembled in a network associated with 11 miRNAs altered in OS in this study. Eight genes were downregulated and 21 upregulated. Red: downregulated; blue: upregulated. Gray lines: association between genes; Red lines: association between gene and miRNA; continuous line: direct association; dashed line: indirect association.

    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Discovery Cohort 23 OS samples (Fresh frozen) Normal control: 3 OB cell lines Validation Cohort 78 OS samples (FFPE) Normal control: 3 OB cell lines Cohorts RNA extraction cDNA synthesis Quality control miRNA analysis Data analysis miRNeasy Mini Kit (Qiagen) miRNAeasy FFPE Kit (Qiagen) 50 ng RNA Universal cDNA Synthesis Kit II (Exiqon) 65 ng RNA Universal cDNA Synthesis Kit II (Exiqon) QC of cDNA Expression of: miR-103; miR-191-5p; miR-423 (Cq<32) UniSp6 (Spike-in) (Cq:16-18) QC of cDNA Expression of: miR-191-5p; miR-423-5p (Cq<30); UniSP4, UniSp5, UniSp6 (Spike-ins) 752 miRNAs analysed microRNA Ready-to-Use PCR, Human Panel I+II v2 (Exiqon) cDNA diluted 1:100 33 miRNAs analysed Pick&Mix microRNA PCR Panels (Exiqon) cDNA diluted 1:20 Analysis in GenEx Software (MultiD) Normalization: Mean global expression Analysis in GenEx Software (MultiD) Normalization: 5 reference miRs 78 miRs diff. expressed (OS samples vs OB cell lines) 5 reference miRs identified 29 miRs diff. expressed (OS samples vs OB cell lines) 33 miRs diff. expressed Exclusion of miRs with no expr. diff. btw.

    Techniques: