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light sheet microscope  (Olympus)


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    Structured Review

    Olympus light sheet microscope
    Light Sheet Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/light+sheet+microscope/Alpha3+Light+Sheet+Microscope/pm41213312-178-19-22
    Average 93 stars, based on 20 article reviews
    light sheet microscope - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: GliaTrap is a biodegradable, non-swelling and non-inflammatory hydrogel with tuned release of CXCL12 to attract migrating glioblastoma cells
    Article Snippet: .. Following X-CLARITY, the processed brains were imaged using a 3D light-sheet microscope (Olympus). ..

    Article Title: Layer 5 myelination gates corticothalamic coincidence detection
    Article Snippet: .. The brain and spinal cord were imaged with an Ultramicroscope II (LaVision BioTec) light sheet microscope equipped with an MVX-10 Zoom Body (Olympus), MVPLAPO 2× Objective lens (Olympus), Neo sCMOS camera (Andor) (2560×2160 pixels. .. Pixel size: 6.5×6.5 μm2) and Imspector software (version V380) (LaVision BioTec).

    Article Title: GliaTrap is a biodegradable, non-swelling and non-inflammatory hydrogel with tuned release of CXCL12 to attract migrating glioblastoma cells
    Article Snippet: .. Following tissue clearing, the brains were stained for smooth muscle actin to label blood vessels and imaged with a 3D light sheet microscope (Olympus). ..

    Article Title: Optical sectioning methods in three-dimensional bioimaging
    Article Snippet: .. Based on the optical sectioning model mentioned earlier and the assumption of ideal illumination, the optical sectioning strength of specific techniques can be represented by the FWHM of Eq. , as shown in Fig. . \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$I(u)=\left\{\begin{array}{l}\int {T}^{2}(s,u)jinc(\beta s)sds\,{\rm{Confocal}}\\ \int {T}^{2}({s}_{x},0,u)\sin c(\beta {s}_{x}/\pi )d{s}_{x}\,{{\rm{Line}}\,{\rm{Confocal}}}\\ \int T(s/2,u/2)T(s,u)sds\,{\rm{Two}}-{\rm{photon}}\\ \exp (-{u}^{2}/2{c}^{2})T({s}_{x},{s}_{y},u)\delta ({s}_{x},{s}_{y})\,{{\rm{Light}}\,{\rm{sheet}}}\\ \int \delta ({s}_{x}-{{\rm{s}}}_{0})T({s}_{x},0,u)d{s}_{x}\,{{\rm{Structured}}\,{\rm{illumination}}}\\ \int {T}^{2}({s}_{x},0,u)\sin c(\beta {s}_{x}/\pi )[1-\,\cos (2\beta {s}_{x})]d{s}_{x}\,{{\rm{Natural}}\,{\rm{illumination}}}\end{array}\right.$$\end{document} I ( u ) = ∫ T 2 ( s , u ) j i n c ( β s ) s d s Confocal ∫ T 2 ( s x , 0 , u ) sin c ( β s x / π ) d s x Line Confocal ∫ T ( s / 2 , u / 2 ) T ( s , u ) s d s Two − photon exp ( − u 2 / 2 c 2 ) T ( s x , s y , u ) δ ( s x , s y ) Light sheet ∫ δ ( s x − s 0 ) T ( s x , 0 , u ) d s x Structured illumination ∫ T 2 ( s x , 0 , u ) sin c ( β s x / π ) [ 1 − cos ( 2 β s x ) ] d s x Natural illumination where the light sheet microscope employs a 20× detection objective (NA 1.0, XLUMPLFLN 20XW, Olympus) and a 25× illumination objective (NA 1.0, XLSLPLN25XGMP, Olympus) with a working distance of 8 mm. ..

    Article Title: A zebrafish rbm24a-GFP knock-in line for monitoring lineage-specific dynamic protein expression and function.
    Article Snippet: The RNA-binding protein Rbm24 is evolutionarily conserved, and its coding gene displays tissue-specific expression in vertebrates.. However, the dynamic localization of this protein in different cell lineages remains elusive.. We have generated a zebrafish rbm24a-GFP knock-in line in which endogenous Rbm24a is tagged with GFP, allowing the precise monitoring and systematic characterization of its spatiotemporal expression and subcellular localization during development and in the adult.

    Article Title: Application of the Magnet-Cre optogenetic system in the chicken model.
    Article Snippet: Chickens serve as an excellent model organism for developmental biology, offering unique opportunities for precise spatiotemporal access to embryos within eggs.. Optogenes are light-activated proteins that regulate gene expression, offering a non-invasive method to activate genes at specific locations and developmental stages, advancing developmental biology research.. This study employed the Magnet-Cre optogenetic system to control gene expression in developing chicken embryos.

    Article Title: Voltage imaging reveals circuit computations in the raphe underlying serotonin-mediated motor vigor learning
    Article Snippet: .. The zebrafish was further mounted to a custom-made chamber using 2% agarose (Sigma-Aldrich, A9414) and placed under a light-sheet microscope with a 20x objective lens (Olympus, XLUMPLFLN). ..

    Staining:

    Article Title: GliaTrap is a biodegradable, non-swelling and non-inflammatory hydrogel with tuned release of CXCL12 to attract migrating glioblastoma cells
    Article Snippet: .. Following tissue clearing, the brains were stained for smooth muscle actin to label blood vessels and imaged with a 3D light sheet microscope (Olympus). ..

    Imaging:

    Article Title: A zebrafish rbm24a-GFP knock-in line for monitoring lineage-specific dynamic protein expression and function.
    Article Snippet: The RNA-binding protein Rbm24 is evolutionarily conserved, and its coding gene displays tissue-specific expression in vertebrates.. However, the dynamic localization of this protein in different cell lineages remains elusive.. We have generated a zebrafish rbm24a-GFP knock-in line in which endogenous Rbm24a is tagged with GFP, allowing the precise monitoring and systematic characterization of its spatiotemporal expression and subcellular localization during development and in the adult.

    Article Title: Application of the Magnet-Cre optogenetic system in the chicken model.
    Article Snippet: Chickens serve as an excellent model organism for developmental biology, offering unique opportunities for precise spatiotemporal access to embryos within eggs.. Optogenes are light-activated proteins that regulate gene expression, offering a non-invasive method to activate genes at specific locations and developmental stages, advancing developmental biology research.. This study employed the Magnet-Cre optogenetic system to control gene expression in developing chicken embryos.

    Article Title: A sensorimotor instability drives a locomotor transition during fish development
    Article Snippet: .. Lightsheet imaging was performed with the Alpha3 system (see tab.1), equipped with a 10× XL Plan N objective (XLPLN10XSVMP, Olympus, Japan) with a refractive index adaptive collar (RI 1.33–1.52), and an sCMOS Orca Flash4 camera (Hamamatsu, Japan). ..

    Refractive Index:

    Article Title: A sensorimotor instability drives a locomotor transition during fish development
    Article Snippet: .. Lightsheet imaging was performed with the Alpha3 system (see tab.1), equipped with a 10× XL Plan N objective (XLPLN10XSVMP, Olympus, Japan) with a refractive index adaptive collar (RI 1.33–1.52), and an sCMOS Orca Flash4 camera (Hamamatsu, Japan). ..



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    Olympus light sheet microscopy
    (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence <t>microscopy.</t> Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.
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    (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence <t>microscopy.</t> Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.
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    (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence <t>microscopy.</t> Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.
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    Image Search Results


    (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence microscopy. Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.

    Journal: bioRxiv

    Article Title: Most early-born subplate neurons persist as Layer 6b neurons in the adult mouse neocortex

    doi: 10.1101/2025.11.20.689634

    Figure Lengend Snippet: (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence microscopy. Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.

    Article Snippet: Cleared whole brain tissues were imaged by Light sheet microscopy (Olympus, MVX10-LS), and the z-axis interval was 4 μm in the P0 sample and 9 μm in the 8 weeks.

    Techniques: Immunohistochemical staining, Expressing, Fluorescence, Microscopy