odyssey emsa buffer kit (LI-COR)
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odyssey emsa buffer kit
Odyssey Emsa Buffer Kit, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/licor+intercept+blocking+buffer/ODYSSEY+EMSA+BUFFER+KIT/pmc04832313-92-13-17
Average 98 stars, based on 89 article reviews
Odyssey Emsa Buffer Kit, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/licor+intercept+blocking+buffer/ODYSSEY+EMSA+BUFFER+KIT/pmc04832313-92-13-17
Average 98 stars, based on 89 article reviews
odyssey emsa buffer kit - by Bioz Stars,
2026-10
98/100 stars
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other:Article Title: TIPIN coordinates ATM-dependent checkpoint and NF-κB signaling to counteract DNA replication damage from topoisomerase inhibition. Article Snippet: Following three additional washes with PBS, coverslips were mounted onto glass microscope slides using VectaShield Antifade mounting medium containing DAPI (Vector Lab). Binding Assay:Article Title: Genetic regulation of the estrogen receptor and inherited predisposition to breast cancer. Article Snippet: Probe and competitor duplexes were annealed by mixing complementary oligonucleotides (IDT), heating 3 min at 95 °C and cooling gradually to room temperature. .. Binding reactions were performed with the Article Title: HNF1B-MODY in the Norwegian MODY Registry and the Norwegian Childhood Diabetes Registry: Clinical insights and prevalence informed by genetic and functional evaluation Article Snippet: .. Optimal binding conditions were facilitated using the Labeling:Article Title: Genetic regulation of the estrogen receptor and inherited predisposition to breast cancer. Article Snippet: Probe and competitor duplexes were annealed by mixing complementary oligonucleotides (IDT), heating 3 min at 95 °C and cooling gradually to room temperature. .. Binding reactions were performed with the Article Title: Cytoplasmic PXR regulates glucose metabolism by binding mRNAs and modulating their stability Article Snippet: .. The recombinant PXR proteins (Cusabio, CSB-EP016048HU), RNH1 proteins (Cusabio, CSB-EP019901HUb1) or synthesized peptides (GenScript) were incubated with 200 nM of labeled RNAs for 30 min at room temperature according to the guidelines of the Incubation:Article Title: Genetic regulation of the estrogen receptor and inherited predisposition to breast cancer. Article Snippet: Probe and competitor duplexes were annealed by mixing complementary oligonucleotides (IDT), heating 3 min at 95 °C and cooling gradually to room temperature. .. Binding reactions were performed with the Article Title: Cytoplasmic PXR regulates glucose metabolism by binding mRNAs and modulating their stability Article Snippet: .. The recombinant PXR proteins (Cusabio, CSB-EP016048HU), RNH1 proteins (Cusabio, CSB-EP019901HUb1) or synthesized peptides (GenScript) were incubated with 200 nM of labeled RNAs for 30 min at room temperature according to the guidelines of the Article Title: Integrative characterization of MYC RNA-binding function Article Snippet: For MYC protein, at room temperature, the inclusion bodies in pellets were collected, dissolved into 30 mL of denaturing lysis buffer (20 mM Tris-HCl pH 8.0, 8 M urea, 500 mM NaCl, 10 mM imidazole, 0.5% NP-40, 1 mM PMSF) overnight, clarified in 10,000 rpm, and incubated with 1 mL of HisPur Ni-NTA resin for 1 h. After five washes with washing buffer (20 mM Tris-HCl pH 8.0, 8 M urea, 500 mM NaCl, 30 mM imidazole, 0.5% NP-40, 1 mM β-mercaptoethanol, 1 mM PMSF), recombinant His-MYC was eluted from HisPur Ni-NTA resin with 1 mL of elution buffer (20 mM Tris-HCl pH 8.0, 8 M urea, 500 mM NaCl, 500 mM imidazole, 0.5% NP-40, 1 mM PMSF) and dialyzed in the Slide-A-Lyzer Dialysis Cassettes (ThermoFisher, 66380) against renaturation buffer (20 mM Tris-HCl pH 8.0, 500 mM KCl, 10% glycerol, 0.05% NP-40, 1 mM DTT, 0.2 mM PMSF) containing a gradient of urea from 4 M to 0 M for 2 h, respectively. .. A series of diluted recombinant MYC proteins and their derivatives were incubated with 200 nM of fluor-labeled RNA for 30 min at room temperature, following the guidelines of an Article Title: TIPIN coordinates ATM-dependent checkpoint and NF-κB signaling to counteract DNA replication damage from topoisomerase inhibition Article Snippet: Images were captured using an Eclipse Ts2R-FL inverted fluorescence microscope (Nikon) equipped with a Nikon DS-Qi2 digital camera. .. EMSA was performed as previously described using the Nucleic Acid Electrophoresis:Article Title: HNF1B-MODY in the Norwegian MODY Registry and the Norwegian Childhood Diabetes Registry: Clinical insights and prevalence informed by genetic and functional evaluation Article Snippet: .. Optimal binding conditions were facilitated using the Imaging:Article Title: HNF1B-MODY in the Norwegian MODY Registry and the Norwegian Childhood Diabetes Registry: Clinical insights and prevalence informed by genetic and functional evaluation Article Snippet: .. Optimal binding conditions were facilitated using the Recombinant:Article Title: Cytoplasmic PXR regulates glucose metabolism by binding mRNAs and modulating their stability Article Snippet: .. The recombinant PXR proteins (Cusabio, CSB-EP016048HU), RNH1 proteins (Cusabio, CSB-EP019901HUb1) or synthesized peptides (GenScript) were incubated with 200 nM of labeled RNAs for 30 min at room temperature according to the guidelines of the Article Title: Integrative characterization of MYC RNA-binding function Article Snippet: For MYC protein, at room temperature, the inclusion bodies in pellets were collected, dissolved into 30 mL of denaturing lysis buffer (20 mM Tris-HCl pH 8.0, 8 M urea, 500 mM NaCl, 10 mM imidazole, 0.5% NP-40, 1 mM PMSF) overnight, clarified in 10,000 rpm, and incubated with 1 mL of HisPur Ni-NTA resin for 1 h. After five washes with washing buffer (20 mM Tris-HCl pH 8.0, 8 M urea, 500 mM NaCl, 30 mM imidazole, 0.5% NP-40, 1 mM β-mercaptoethanol, 1 mM PMSF), recombinant His-MYC was eluted from HisPur Ni-NTA resin with 1 mL of elution buffer (20 mM Tris-HCl pH 8.0, 8 M urea, 500 mM NaCl, 500 mM imidazole, 0.5% NP-40, 1 mM PMSF) and dialyzed in the Slide-A-Lyzer Dialysis Cassettes (ThermoFisher, 66380) against renaturation buffer (20 mM Tris-HCl pH 8.0, 500 mM KCl, 10% glycerol, 0.05% NP-40, 1 mM DTT, 0.2 mM PMSF) containing a gradient of urea from 4 M to 0 M for 2 h, respectively. .. A series of diluted recombinant MYC proteins and their derivatives were incubated with 200 nM of fluor-labeled RNA for 30 min at room temperature, following the guidelines of an Synthesized:Article Title: Cytoplasmic PXR regulates glucose metabolism by binding mRNAs and modulating their stability Article Snippet: .. The recombinant PXR proteins (Cusabio, CSB-EP016048HU), RNH1 proteins (Cusabio, CSB-EP019901HUb1) or synthesized peptides (GenScript) were incubated with 200 nM of labeled RNAs for 30 min at room temperature according to the guidelines of the |