odyssey blocking buffer (LI-COR)
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odyssey blocking buffer
Odyssey Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1324 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/licor+blocking+buffer/DONKEYaMOUSE+IRDye+680RD/pmc04798913-259-29-32
Average 99 stars, based on 1324 article reviews
Odyssey Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1324 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/licor+blocking+buffer/DONKEYaMOUSE+IRDye+680RD/pmc04798913-259-29-32
Average 99 stars, based on 1324 article reviews
odyssey blocking buffer - by Bioz Stars,
2026-10
99/100 stars
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Blocking Assay:Article Title: USB1 deficiency disrupts neutrophil maturation via RNA dysregulation independent of global pre-mRNA splicing. Article Snippet: .. After treatment with the Article Title: Astrocytes connect specific brain regions through plastic networks. Article Snippet: After a 1 h block (LI-COR Intercept PBS blocking buffer (LI-COR 927-70001)), the blots were incubated with rabbit anti-Cx43 (1:1,000; Cell Signaling, 3512) and mouse anti-actin (1:1,000; Abcam, ab8226) antibodies in LI-COR Intercept PBS blocking buffer (LI-COR 927-70001) with 0.1% Tween-20 (Sigma-Aldrich, P9416) for 4 h, then washed four times for 5 min with PBST. .. They were then incubated for 1 h in LI-COR Intercept PBS blocking buffer (LI-COR 927-70001) containing 0.1% Tween-20 and 0.1% SDS with Membrane:Article Title: Role of Collection Media on the Biological Activity of Extracellular Vesicles From hTERT‐Immortalised Mesenchymal Stromal Cells Article Snippet: The following primary antibodies were used: TSG101, abcam, ab125011, 1:1,000; Syntenin‐1, Origene, TA504796, 1:1,000; Calnexin, GeneTex, GTX101676, 1:1000, CD81 (B‐11), Santa Cruz, sc‐16602. .. The membrane was washed three times with TBS (Thermo Fisher Scientific) mixed with 0.1% Tween‐20 (TBS‐T) before being incubated with the relevant Article Title: Small-molecule binding-site discovery using silyl ether-enabled chemoproteomics. Article Snippet: After blocking, the membrane was incubated with one of the rabbit primary antibodies listed below at a ratio of 1:3,000 in 5% (w/v) milk in 1× TBS overnight at 4 °C, washed with 1× TBS for 10 min three times the following day and incubated with a secondary antibody, IRDye 800CW goat anti-rabbit secondary antibody (Li-Cor Biotechnology, 926-32211, #D50528-07), at a ratio of 1:5,000 in 5% (w/v) milk in 1× TBST (TBS with 0.1% Tween20) room temperature for 1 h. After secondary antibody incubation, the membrane was washed with 1× TBS for 10 min three times and imaged using a Bio-Rad ChemiDoc Imaging System to obtain western blot results. .. For loading control, the membrane underwent the similar western blot analysis as described above using mouse anti-β-actin antibody (Cell Signaling, #3700S, #21) or mouse GAPDH monoclonal antibody (Proteintech, #60004-1-Ig, #10080731) as a primary antibody at 1:3,000 dilution and Incubation:Article Title: Role of Collection Media on the Biological Activity of Extracellular Vesicles From hTERT‐Immortalised Mesenchymal Stromal Cells Article Snippet: The following primary antibodies were used: TSG101, abcam, ab125011, 1:1,000; Syntenin‐1, Origene, TA504796, 1:1,000; Calnexin, GeneTex, GTX101676, 1:1000, CD81 (B‐11), Santa Cruz, sc‐16602. .. The membrane was washed three times with TBS (Thermo Fisher Scientific) mixed with 0.1% Tween‐20 (TBS‐T) before being incubated with the relevant Article Title: Astrocytes connect specific brain regions through plastic networks. Article Snippet: After a 1 h block (LI-COR Intercept PBS blocking buffer (LI-COR 927-70001)), the blots were incubated with rabbit anti-Cx43 (1:1,000; Cell Signaling, 3512) and mouse anti-actin (1:1,000; Abcam, ab8226) antibodies in LI-COR Intercept PBS blocking buffer (LI-COR 927-70001) with 0.1% Tween-20 (Sigma-Aldrich, P9416) for 4 h, then washed four times for 5 min with PBST. .. They were then incubated for 1 h in LI-COR Intercept PBS blocking buffer (LI-COR 927-70001) containing 0.1% Tween-20 and 0.1% SDS with Control:Article Title: Small-molecule binding-site discovery using silyl ether-enabled chemoproteomics. Article Snippet: After blocking, the membrane was incubated with one of the rabbit primary antibodies listed below at a ratio of 1:3,000 in 5% (w/v) milk in 1× TBS overnight at 4 °C, washed with 1× TBS for 10 min three times the following day and incubated with a secondary antibody, IRDye 800CW goat anti-rabbit secondary antibody (Li-Cor Biotechnology, 926-32211, #D50528-07), at a ratio of 1:5,000 in 5% (w/v) milk in 1× TBST (TBS with 0.1% Tween20) room temperature for 1 h. After secondary antibody incubation, the membrane was washed with 1× TBS for 10 min three times and imaged using a Bio-Rad ChemiDoc Imaging System to obtain western blot results. .. For loading control, the membrane underwent the similar western blot analysis as described above using mouse anti-β-actin antibody (Cell Signaling, #3700S, #21) or mouse GAPDH monoclonal antibody (Proteintech, #60004-1-Ig, #10080731) as a primary antibody at 1:3,000 dilution and Western Blot:Article Title: Small-molecule binding-site discovery using silyl ether-enabled chemoproteomics. Article Snippet: After blocking, the membrane was incubated with one of the rabbit primary antibodies listed below at a ratio of 1:3,000 in 5% (w/v) milk in 1× TBS overnight at 4 °C, washed with 1× TBS for 10 min three times the following day and incubated with a secondary antibody, IRDye 800CW goat anti-rabbit secondary antibody (Li-Cor Biotechnology, 926-32211, #D50528-07), at a ratio of 1:5,000 in 5% (w/v) milk in 1× TBST (TBS with 0.1% Tween20) room temperature for 1 h. After secondary antibody incubation, the membrane was washed with 1× TBS for 10 min three times and imaged using a Bio-Rad ChemiDoc Imaging System to obtain western blot results. .. For loading control, the membrane underwent the similar western blot analysis as described above using mouse anti-β-actin antibody (Cell Signaling, #3700S, #21) or mouse GAPDH monoclonal antibody (Proteintech, #60004-1-Ig, #10080731) as a primary antibody at 1:3,000 dilution and other:Article Title: Anti-TDP-43 binding molecules and uses thereof Article Snippet: Secondary Staining:Article Title: CD4 + T cell activation is dependent on a novel form of ULK1/2-independent autophagy Article Snippet: Membranes were incubated 24 h at 4°C, in 3% BSA/TBST with primary antibodies LC3B (MBL, PM036, 1:1000), LC3B (NanoTools, 0231-100, 1:1000), S6 (54D2) (Cell Signaling, 2317, 1:1000), pS6 s235/236 (Cell Signaling, 2211, 1:1000), β-Actin (C4) (Santa Cruz, sc-47778, 1:1000), AMPKα (F6) (Cell Signaling, 2793, 1:1000), pAMPKα t172 (Cell Signaling, 2531, 1:1000), ULK1 (D8H5) (Cell Signaling, 8054, 1:500), pULK1 s757 (Cell Signaling, 6888, 1:500), pULK1 s555 (D1H4) (Cell Signaling, 5869, 1:500), ATG14 (Cell Signaling, 5504, 1:500), pATG14 s29 (Cell Signaling, 13155, 1:1000), ACC (C83B10) (Cell Signaling, 3676, 1:500), pACC s79 (D7D11) (Cell Signaling, 11818, 1:500), AKT (Cell Signaling, 9272, 1:1000), pAKT s473 (Cell Signaling, 9271, 1:1000), Vinculin (7F9) (Thermo Fisher Scientific, 14-9777-82, 1:5000), ATG13 (D4P1K) (Cell Signaling, 13273, 1:1000) or Beclin 1 (Cell Signaling, 3738, 1:1000). .. After washing with TBST, membranes were stained for 1 h at room temperature in 5% milk/TBST with secondary antibodies rabbit anti-mouse immunoglobulin-HRP (Dako, P0260, 1:5000), swine anti-rabbit immunoglobulin-HRP (Dako, P0217, 1:5000) or |