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library prep kit  (New England Biolabs)


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    Structured Review

    New England Biolabs library prep kit
    Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/libra/NEBNext+Ultra+II+FS+DNA+PCR-free+Libra/pm42076830-126-23-16
    Average 97 stars, based on 263 article reviews
    library prep kit - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Purification:

    Article Title: Neonatal overfeeding accelerates age-related sarcopenia and metabolic disorders through lifelong gut mucosa and microbiota programming in mice
    Article Snippet: Microbial genomic DNA was extracted using the DNA Stool Mini Kit (TIANGEN, DP328), and the V3–V4 hypervariable regions of the 16S rRNA gene were amplified using universal primers (515F/806R). .. Purified amplicons were used to construct sequencing libraries with the NEB Next Ultra II FS DNA PCR-free Library Prep Kit (New England Biolabs, E7430L) and subjected to paired-end sequencing on an Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA) through Novogene Co., Ltd (Novogene, Beijing, China). ..

    Article Title: Decoding serotonin in endometriosis: unveiling its role in disease pathogenesis via the gut-reproductive microbiota axis.
    Article Snippet: STUDY QUESTION: How can the potential mechanisms and targets of endometriosis be explored through multi-omics and multilocation approaches?. SUMMARY ANSWER: This exploration of the gut–reproductive axis in patients with endometriosis found that serotonin is elevated in endometriosis and promotes disease progression through enhanced cell proliferation and inflammation.. WHAT IS KNOWN ALREADY: Endometriosis is a common inflammatory disease.

    Construct:

    Article Title: Neonatal overfeeding accelerates age-related sarcopenia and metabolic disorders through lifelong gut mucosa and microbiota programming in mice
    Article Snippet: Microbial genomic DNA was extracted using the DNA Stool Mini Kit (TIANGEN, DP328), and the V3–V4 hypervariable regions of the 16S rRNA gene were amplified using universal primers (515F/806R). .. Purified amplicons were used to construct sequencing libraries with the NEB Next Ultra II FS DNA PCR-free Library Prep Kit (New England Biolabs, E7430L) and subjected to paired-end sequencing on an Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA) through Novogene Co., Ltd (Novogene, Beijing, China). ..

    Article Title: Effects of Aged Polymethylmethacrylate Microplastics on Physiological Biochemistry and Intestinal-Sediment Microbial Communities of Urechis unicinctus (von Drasche, 1881).
    Article Snippet: .. The PCR program consisted of an initial denaturation at 95 ◦C for 3 min, 35 cycles of 95 ◦C for 30 s, 55 ◦C for 30 s and 72 ◦C for 45 s, and a final extension at 72 ◦C for 10 min. Amplicons were checked on a 2% agarose gel, and sequencing libraries were constructed with the NEB Next Ultra II FS DNA PCR-free Library Prep Kit (New England Biolabs, Ipswich, MA, USA). .. The constructed libraries were quantified using Qubit 4 Fluorometer and qPCR. (https: //www.thermofisher.com, accessed 10 August 2026) Qualified libraries were sequenced on the NovaSeq 6000 platform with PE250 reads.

    Article Title: Decoding serotonin in endometriosis: unveiling its role in disease pathogenesis via the gut-reproductive microbiota axis.
    Article Snippet: STUDY QUESTION: How can the potential mechanisms and targets of endometriosis be explored through multi-omics and multilocation approaches?. SUMMARY ANSWER: This exploration of the gut–reproductive axis in patients with endometriosis found that serotonin is elevated in endometriosis and promotes disease progression through enhanced cell proliferation and inflammation.. WHAT IS KNOWN ALREADY: Endometriosis is a common inflammatory disease.

    Article Title: Salinity-adapted and core microbiota along the soil salinity gradients encircling the Tarim Basin
    Article Snippet: .. Sequencing libraries were then constructed and indexed using the NEB Next Ultra II FS DNA PCR-free Library Prep Kit (NEB/E7430L). ..

    Article Title: Extracellular Vesicle-Encapsulated Small RNAs From Clostridium Butyricum Modulate Host Intestinal Lipid Metabolism.
    Article Snippet: CR reactions were performed with Phusion High-Fidelity PCR aster Mix (New England Biolabs, Shanghai, China). .. Sequencing ibraries were constructed using the NEB Next Ultra II FS NA PCR-free Library Prep Kit (New England Biolabs). ..

    Sequencing:

    Article Title: Neonatal overfeeding accelerates age-related sarcopenia and metabolic disorders through lifelong gut mucosa and microbiota programming in mice
    Article Snippet: Microbial genomic DNA was extracted using the DNA Stool Mini Kit (TIANGEN, DP328), and the V3–V4 hypervariable regions of the 16S rRNA gene were amplified using universal primers (515F/806R). .. Purified amplicons were used to construct sequencing libraries with the NEB Next Ultra II FS DNA PCR-free Library Prep Kit (New England Biolabs, E7430L) and subjected to paired-end sequencing on an Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA) through Novogene Co., Ltd (Novogene, Beijing, China). ..

    Article Title: Effects of Aged Polymethylmethacrylate Microplastics on Physiological Biochemistry and Intestinal-Sediment Microbial Communities of Urechis unicinctus (von Drasche, 1881).
    Article Snippet: .. The PCR program consisted of an initial denaturation at 95 ◦C for 3 min, 35 cycles of 95 ◦C for 30 s, 55 ◦C for 30 s and 72 ◦C for 45 s, and a final extension at 72 ◦C for 10 min. Amplicons were checked on a 2% agarose gel, and sequencing libraries were constructed with the NEB Next Ultra II FS DNA PCR-free Library Prep Kit (New England Biolabs, Ipswich, MA, USA). .. The constructed libraries were quantified using Qubit 4 Fluorometer and qPCR. (https: //www.thermofisher.com, accessed 10 August 2026) Qualified libraries were sequenced on the NovaSeq 6000 platform with PE250 reads.

    Article Title: Salinity-adapted and core microbiota along the soil salinity gradients encircling the Tarim Basin
    Article Snippet: .. Sequencing libraries were then constructed and indexed using the NEB Next Ultra II FS DNA PCR-free Library Prep Kit (NEB/E7430L). ..

    Article Title: Phylogenomics and museomics reveal five distinct species of tiger cats in South America.
    Article Snippet: Article Phylogenomics and museomics reveal five distinct species of tiger cats in South America

    Article Title: Extracellular Vesicle-Encapsulated Small RNAs From Clostridium Butyricum Modulate Host Intestinal Lipid Metabolism.
    Article Snippet: CR reactions were performed with Phusion High-Fidelity PCR aster Mix (New England Biolabs, Shanghai, China). .. Sequencing ibraries were constructed using the NEB Next Ultra II FS NA PCR-free Library Prep Kit (New England Biolabs). ..

    Polymerase Chain Reaction:

    Article Title: Neonatal overfeeding accelerates age-related sarcopenia and metabolic disorders through lifelong gut mucosa and microbiota programming in mice
    Article Snippet: Microbial genomic DNA was extracted using the DNA Stool Mini Kit (TIANGEN, DP328), and the V3–V4 hypervariable regions of the 16S rRNA gene were amplified using universal primers (515F/806R). .. Purified amplicons were used to construct sequencing libraries with the NEB Next Ultra II FS DNA PCR-free Library Prep Kit (New England Biolabs, E7430L) and subjected to paired-end sequencing on an Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA) through Novogene Co., Ltd (Novogene, Beijing, China). ..

    Article Title: Effects of Aged Polymethylmethacrylate Microplastics on Physiological Biochemistry and Intestinal-Sediment Microbial Communities of Urechis unicinctus (von Drasche, 1881).
    Article Snippet: .. The PCR program consisted of an initial denaturation at 95 ◦C for 3 min, 35 cycles of 95 ◦C for 30 s, 55 ◦C for 30 s and 72 ◦C for 45 s, and a final extension at 72 ◦C for 10 min. Amplicons were checked on a 2% agarose gel, and sequencing libraries were constructed with the NEB Next Ultra II FS DNA PCR-free Library Prep Kit (New England Biolabs, Ipswich, MA, USA). .. The constructed libraries were quantified using Qubit 4 Fluorometer and qPCR. (https: //www.thermofisher.com, accessed 10 August 2026) Qualified libraries were sequenced on the NovaSeq 6000 platform with PE250 reads.

    Article Title: Decoding serotonin in endometriosis: unveiling its role in disease pathogenesis via the gut-reproductive microbiota axis.
    Article Snippet: STUDY QUESTION: How can the potential mechanisms and targets of endometriosis be explored through multi-omics and multilocation approaches?. SUMMARY ANSWER: This exploration of the gut–reproductive axis in patients with endometriosis found that serotonin is elevated in endometriosis and promotes disease progression through enhanced cell proliferation and inflammation.. WHAT IS KNOWN ALREADY: Endometriosis is a common inflammatory disease.

    Article Title: Salinity-adapted and core microbiota along the soil salinity gradients encircling the Tarim Basin
    Article Snippet: .. Sequencing libraries were then constructed and indexed using the NEB Next Ultra II FS DNA PCR-free Library Prep Kit (NEB/E7430L). ..

    Article Title: Phylogenomics and museomics reveal five distinct species of tiger cats in South America.
    Article Snippet: Article Phylogenomics and museomics reveal five distinct species of tiger cats in South America

    Article Title: Toxic metals differentially affect bacterial and fungal seed endophytes in Arabidopsis arenosa
    Article Snippet: PCR was performed in the following conditions: initial denaturation at 98 °C for 1 min, followed by 30 cycles of denaturation at 98 °C for 10 s, annealing at 50 °C for 30 s, and elongation at 72 °C for 30 s and final elongation at 72 °C for 5 min. PCR products were visualised in agarose gel and purified with Universal DNA Purification Kit (TianGen, China). .. DNA libraries were generated using NEB Next Ultra II FS DNA PCR-free Library Prep Kit (New England Biolabs, USA) following manufacturer’s instruction. ..

    Article Title: Extracellular Vesicle-Encapsulated Small RNAs From Clostridium Butyricum Modulate Host Intestinal Lipid Metabolism.
    Article Snippet: CR reactions were performed with Phusion High-Fidelity PCR aster Mix (New England Biolabs, Shanghai, China). .. Sequencing ibraries were constructed using the NEB Next Ultra II FS NA PCR-free Library Prep Kit (New England Biolabs). ..

    other:

    Article Title: Straw-derived dissolved organic matter characteristics determine soil microorganism utilization strategies
    Article Snippet: Library construction was accomplished by the PCR-free NEB Next UltraII FS DNA Library Prep Kit (New England Biolabs, USA).

    Agarose Gel Electrophoresis:

    Article Title: Effects of Aged Polymethylmethacrylate Microplastics on Physiological Biochemistry and Intestinal-Sediment Microbial Communities of Urechis unicinctus (von Drasche, 1881).
    Article Snippet: .. The PCR program consisted of an initial denaturation at 95 ◦C for 3 min, 35 cycles of 95 ◦C for 30 s, 55 ◦C for 30 s and 72 ◦C for 45 s, and a final extension at 72 ◦C for 10 min. Amplicons were checked on a 2% agarose gel, and sequencing libraries were constructed with the NEB Next Ultra II FS DNA PCR-free Library Prep Kit (New England Biolabs, Ipswich, MA, USA). .. The constructed libraries were quantified using Qubit 4 Fluorometer and qPCR. (https: //www.thermofisher.com, accessed 10 August 2026) Qualified libraries were sequenced on the NovaSeq 6000 platform with PE250 reads.

    Generated:

    Article Title: Toxic metals differentially affect bacterial and fungal seed endophytes in Arabidopsis arenosa
    Article Snippet: PCR was performed in the following conditions: initial denaturation at 98 °C for 1 min, followed by 30 cycles of denaturation at 98 °C for 10 s, annealing at 50 °C for 30 s, and elongation at 72 °C for 30 s and final elongation at 72 °C for 5 min. PCR products were visualised in agarose gel and purified with Universal DNA Purification Kit (TianGen, China). .. DNA libraries were generated using NEB Next Ultra II FS DNA PCR-free Library Prep Kit (New England Biolabs, USA) following manufacturer’s instruction. ..



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