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synthetic escherichia coli codon-optimized gene of the full-length protein l3p mutant  (GenScript corporation)

 
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    GenScript corporation synthetic escherichia coli codon-optimized gene of the full-length protein l3p mutant
    Synthetic Escherichia Coli Codon Optimized Gene Of The Full Length Protein L3p Mutant, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/length+codon/synthetic+escherichia+coli+codon+optimized+gene+of+the+full+length+protein+l3p+mutant/pm40503686-95-27-33
    Average 90 stars, based on 1 article reviews
    synthetic escherichia coli codon-optimized gene of the full-length protein l3p mutant - by Bioz Stars, 2026-09
    90/100 stars

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    Amplification:

    Article Title: Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation.
    Article Snippet: The genes encoding for full-length and truncated versions of N-protein (UniProt: P0DTC9) were polymerase chain reaction (PCR)-amplified from a vector with the complementary DNA of N-protein acquired from Addgene (pGBWm4046785; plasmid #145684). .. The CC-null mutants in which L223, L227, and L230 were mutated to prolines (L3P) were amplified from a synthetic Esc heric hia coli codon-optimized gene of the full-length protein L3P mutant purchased from GenScript, using the primers in Supplementary Table S1 . .. The PCR products encoding N 176–246 , N 1–246 , N 1–365 , and N 1–419 wild-type (WT) (residues 176–246, 1–246, 1–365, and 1–419, respectively; Supplementary Table S2 ) and L3P variants (CC-null) were inserted into the IPTG-inducible pHTP8 vector (NZYTech) fused to an N-terminal TrxA-His 6 tag and a C-terminal Strep-tag.

    Mutagenesis:

    Article Title: Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation.
    Article Snippet: The genes encoding for full-length and truncated versions of N-protein (UniProt: P0DTC9) were polymerase chain reaction (PCR)-amplified from a vector with the complementary DNA of N-protein acquired from Addgene (pGBWm4046785; plasmid #145684). .. The CC-null mutants in which L223, L227, and L230 were mutated to prolines (L3P) were amplified from a synthetic Esc heric hia coli codon-optimized gene of the full-length protein L3P mutant purchased from GenScript, using the primers in Supplementary Table S1 . .. The PCR products encoding N 176–246 , N 1–246 , N 1–365 , and N 1–419 wild-type (WT) (residues 176–246, 1–246, 1–365, and 1–419, respectively; Supplementary Table S2 ) and L3P variants (CC-null) were inserted into the IPTG-inducible pHTP8 vector (NZYTech) fused to an N-terminal TrxA-His 6 tag and a C-terminal Strep-tag.



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