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Qognit Inc legendplextm data analysis software suite
Legendplextm Data Analysis Software Suite, supplied by Qognit Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/legendplextm+data+analysis+software/analysis+based+cloud+data+legendplex+software/pm42156885-368-7-6
Average 86 stars, based on 1 article reviews
legendplextm data analysis software suite - by Bioz Stars, 2026-09
86/100 stars

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Software:

Article Title: Single-cell and spatial transcriptomic analyses of gene therapy-associated retinal inflammation in non-human primates
Article Snippet: Samples were run on the Cytek Aurora’s 5-Laser Full Spectrum Cytometer 16UV-16V-14B-10YG-8R (Fremont, CA, USA), set up as per the manufacturer’s instructions to gate for the specified bead populations ( www.biolegend.com/legendplex ). .. Data were analyzed in the LEGENDplex Data Analysis Software (Qognit, BioLegend). ..

Article Title: Plasma-sprayed 45S5-bioactive glass coating provides superior anti-biofilm activity compared to hydroxyapatite coating while maintaining host cell cytocompatibility and antibacterial immune activation.
Article Snippet: .. Samples were analyzed using the MACSQuant 10 (Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany), and cytokine concentrations were calculated with the LEGENDplexTM Data Analysis Software (https://legendplex.qognit.com). ..

Article Title: Targeting NFATc1-regulated MTHFD2 one-carbon metabolism to suppress sustained T-cell-mediated inflammation in rheumatoid arthritis.
Article Snippet: .. Analysis was performed with the LEGENDplex Data Analysis Software Suire from Qognit. ..

Article Title: Reprogramming tumour-associated macrophages from immune suppressive to inflammatory state by Checkpoint kinase 1 inhibitor combination treatment
Article Snippet: M-CSF levels (740687) in the supernatants were quantified using a LEGENDplex bead-based immunoassay kit from BioLegend, following the manufacturer’s instructions for the LEGENDplex Multi-Analyte Flow Assay Kit. .. Samples were acquired on a BD LSRFortessa X-20 flow cytometer, and cytokine concentrations were calculated using LEGENDplex Qognit analysis software. .. Total RNA was extracted from cell lines using TRIzol (Invitrogen), and cDNA was generated from 2 μg RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). qPCR was performed using PowerUp SYBR Green Master Mix (Applied Biosystems) on a ViiA 7 or QuantStudio 7 Flex system with KiCqStart primers (Merck).

Article Title: The MYB-related transcription factor MYPOP acts as a selective regulator of cancer cell growth.
Article Snippet: Cytofluorometric analyses were performed with a flow cytometer FC500 and CXP analysis software (Beckman Coulter, USA). .. Data analysis was performed with the QOGNIT LEGENDplexTM Data Analysis Software Suite (version 2023- 02-15). .. The protein concentration of IL-24 at time points between 6 h and 54 h post mRNA transfection was quantified using the Human IL-24 DuoSet ELISA Kit (Bio-Techne, USA).

Article Title: Human LFA-1 governs T cell immune surveillance of the skin
Article Snippet: .. Data were analyzed with LEGENDplex Cloud-based Data Analysis Software (Qognit). .. CD14 + monocytes were isolated from freshly collected PBMCs obtained by Ficoll density gradient centrifugation (GE Healthcare).

Article Title: A systemic immune signature stratifies early-stage breast cancer patients and reveals soluble IL-2RA and PD-1 as potential independent prognostic biomarkers.
Article Snippet: Serum samples were analyzed using the LEGENDplexTM protocol and measured with the Northern Lights spectral flow cytometer (Cytek, CA, USA). .. Results were analyzed using the LEGENDplex analysis software (Qognit, San Carlos, CA, USA), based on a standard curve recorded for each factor and run. ..

Flow Cytometry:

Article Title: Reprogramming tumour-associated macrophages from immune suppressive to inflammatory state by Checkpoint kinase 1 inhibitor combination treatment
Article Snippet: M-CSF levels (740687) in the supernatants were quantified using a LEGENDplex bead-based immunoassay kit from BioLegend, following the manufacturer’s instructions for the LEGENDplex Multi-Analyte Flow Assay Kit. .. Samples were acquired on a BD LSRFortessa X-20 flow cytometer, and cytokine concentrations were calculated using LEGENDplex Qognit analysis software. .. Total RNA was extracted from cell lines using TRIzol (Invitrogen), and cDNA was generated from 2 μg RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). qPCR was performed using PowerUp SYBR Green Master Mix (Applied Biosystems) on a ViiA 7 or QuantStudio 7 Flex system with KiCqStart primers (Merck).

FACS:

Article Title: Delayed Inflammatory Resolution and TGF-beta expressing Tregs are associated with healing outcomes in paediatric burns
Article Snippet: Data acquisition was performed on Cytoflex S (Beckman Coulter, Minnesota, USA) using manufacturer’s instructions for gain and gating parameters. .. FACS data was uploaded to BioLegend Qognit software analysis browser (Version 2023-02-15@2019 Qognit, Inc. San Carlos, USA). ..



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