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Fluka Chemical lc ms ultra chromasolv fluka analytical
Lc Ms Ultra Chromasolv Fluka Analytical, supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lc/grade+lc+methanol+ms/pm42319829-136-152-155
Average 86 stars, based on 1 article reviews
lc ms ultra chromasolv fluka analytical - by Bioz Stars, 2026-09
86/100 stars

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Recombinant:

Article Title: The pyruvate transporter hermes regulates autophagy and health by modulating ROS production.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Ref(2)p Abcam Cat# ab178440, RRID:AB_2938801 Rabbit polyclonal anti-phospho-Dros-S6K (Thr398) Cell Signaling Technology Cat# 9209, RRID:AB_2269804 Rabbit polyclonal anti-phospho-PDHA1 (S293) Abcam Cat# ab92696, RRID:AB_10711672 Mouse monoclonal anti-Actin DSHB Cat# jla20, RRID:AB_528068 Chemicals, peptides, and recombinant proteins Agar MoorAgar 41004 Brewer’s Yeast Fisher Scientific ICN90331225 Cornmeal Fisher Scientific ICN90141125 Molasses Thomsen Food Services 02625 Propionic acid Fisher Scientific A258 Phosphoric Acid Fisher Scientific A260 Tegosept Genesee 20-259 UltraPureTM SDS Solution, 10% Invitrogen 15553-035 Tris Base Fisher Scientific BP152-1 Ethylenediaminetetraacetic Acid Fisher Scientific BP120-500 HaltTM Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) Thermo Scientific 78445 4× Laemmli Sample Buffer Bio-Rad 1610747 NovexTM Tris-Glycine Mini Protein Gels, 4–20% Invitrogen XP04202BOX Nitrocellulose Membrane, 0.2 μm Bio-Rad 1620112 PBS (10X), pH 7.4 Gibco 70011044 Paraformaldehyde 16% Aqueous Solution EM Grade EMS 15710 TritonTM X-100 Sigma-Aldrich X100-500ML Normal Goat Serum Gibco PCN5000 VECTASHIELD PLUS Antifade Mounting Medium Vector Laboratories H-1900 Methanol, LC-MS Ultra CHROMASOLV Fluka Analytical 14262-1 L Metabolomics Amino Acid Mix Cambridge Isotope Labs MSK-A2-1.2 PierceTM BCA Protein Assay Kits Thermo Scientific 23227 Water suitable for LC/MS, LiChrosolv Supelco 1153331000 Chloroform HPLC Plus Sigma-Aldrich 650471-1 L Schneider’s Drosophila Medium Thermo Scientific 21720024 Experimental models: Organisms/strains D. melanogaster: GFP-RNAi: w[*]; wg[Sp-1]/CyO; P {w[+mC]=UAS-GFP.RNAi.R}142 Bloomington Drosophila Stock Center RRID:BDSC_44415 D. melanogaster: UAS-Cat: w[1]; P{w[+mC]=UASCat.A}2 Bloomington Drosophila Stock Center RRID:BDSC_24621 D. melanogaster: tub-cyto-roGFP2-Orp1: w[1118]; P{w[+mC]=tub-cyto-roGFP2-Orp1}7/TM3, Sb[1] Bloomington Drosophila Stock Center RRID:BDSC_67671 D. melanogaster: Luciferase-RNAi: y[1] v[1]; P{y [+t7.7] v[+t1.8]=TRiP.JF01355}attP2 Bloomington Drosophila Stock Center RRID:BDSC_31603 D. melanogaster: Df(2 R)BSC696: w[1118]; Df(2 R)BSC696, P + PBac{w[+mC]=XP3.WH3} BSC696/CyO Bloomington Drosophila Stock Center RRID:BDSC_26548 (Continued on next page) 12 Cell Reports 45, 117574, July 28, 2026 ..

Food & Beverages:

Article Title: The pyruvate transporter hermes regulates autophagy and health by modulating ROS production.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Ref(2)p Abcam Cat# ab178440, RRID:AB_2938801 Rabbit polyclonal anti-phospho-Dros-S6K (Thr398) Cell Signaling Technology Cat# 9209, RRID:AB_2269804 Rabbit polyclonal anti-phospho-PDHA1 (S293) Abcam Cat# ab92696, RRID:AB_10711672 Mouse monoclonal anti-Actin DSHB Cat# jla20, RRID:AB_528068 Chemicals, peptides, and recombinant proteins Agar MoorAgar 41004 Brewer’s Yeast Fisher Scientific ICN90331225 Cornmeal Fisher Scientific ICN90141125 Molasses Thomsen Food Services 02625 Propionic acid Fisher Scientific A258 Phosphoric Acid Fisher Scientific A260 Tegosept Genesee 20-259 UltraPureTM SDS Solution, 10% Invitrogen 15553-035 Tris Base Fisher Scientific BP152-1 Ethylenediaminetetraacetic Acid Fisher Scientific BP120-500 HaltTM Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) Thermo Scientific 78445 4× Laemmli Sample Buffer Bio-Rad 1610747 NovexTM Tris-Glycine Mini Protein Gels, 4–20% Invitrogen XP04202BOX Nitrocellulose Membrane, 0.2 μm Bio-Rad 1620112 PBS (10X), pH 7.4 Gibco 70011044 Paraformaldehyde 16% Aqueous Solution EM Grade EMS 15710 TritonTM X-100 Sigma-Aldrich X100-500ML Normal Goat Serum Gibco PCN5000 VECTASHIELD PLUS Antifade Mounting Medium Vector Laboratories H-1900 Methanol, LC-MS Ultra CHROMASOLV Fluka Analytical 14262-1 L Metabolomics Amino Acid Mix Cambridge Isotope Labs MSK-A2-1.2 PierceTM BCA Protein Assay Kits Thermo Scientific 23227 Water suitable for LC/MS, LiChrosolv Supelco 1153331000 Chloroform HPLC Plus Sigma-Aldrich 650471-1 L Schneider’s Drosophila Medium Thermo Scientific 21720024 Experimental models: Organisms/strains D. melanogaster: GFP-RNAi: w[*]; wg[Sp-1]/CyO; P {w[+mC]=UAS-GFP.RNAi.R}142 Bloomington Drosophila Stock Center RRID:BDSC_44415 D. melanogaster: UAS-Cat: w[1]; P{w[+mC]=UASCat.A}2 Bloomington Drosophila Stock Center RRID:BDSC_24621 D. melanogaster: tub-cyto-roGFP2-Orp1: w[1118]; P{w[+mC]=tub-cyto-roGFP2-Orp1}7/TM3, Sb[1] Bloomington Drosophila Stock Center RRID:BDSC_67671 D. melanogaster: Luciferase-RNAi: y[1] v[1]; P{y [+t7.7] v[+t1.8]=TRiP.JF01355}attP2 Bloomington Drosophila Stock Center RRID:BDSC_31603 D. melanogaster: Df(2 R)BSC696: w[1118]; Df(2 R)BSC696, P + PBac{w[+mC]=XP3.WH3} BSC696/CyO Bloomington Drosophila Stock Center RRID:BDSC_26548 (Continued on next page) 12 Cell Reports 45, 117574, July 28, 2026 ..

Membrane:

Article Title: The pyruvate transporter hermes regulates autophagy and health by modulating ROS production.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Ref(2)p Abcam Cat# ab178440, RRID:AB_2938801 Rabbit polyclonal anti-phospho-Dros-S6K (Thr398) Cell Signaling Technology Cat# 9209, RRID:AB_2269804 Rabbit polyclonal anti-phospho-PDHA1 (S293) Abcam Cat# ab92696, RRID:AB_10711672 Mouse monoclonal anti-Actin DSHB Cat# jla20, RRID:AB_528068 Chemicals, peptides, and recombinant proteins Agar MoorAgar 41004 Brewer’s Yeast Fisher Scientific ICN90331225 Cornmeal Fisher Scientific ICN90141125 Molasses Thomsen Food Services 02625 Propionic acid Fisher Scientific A258 Phosphoric Acid Fisher Scientific A260 Tegosept Genesee 20-259 UltraPureTM SDS Solution, 10% Invitrogen 15553-035 Tris Base Fisher Scientific BP152-1 Ethylenediaminetetraacetic Acid Fisher Scientific BP120-500 HaltTM Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) Thermo Scientific 78445 4× Laemmli Sample Buffer Bio-Rad 1610747 NovexTM Tris-Glycine Mini Protein Gels, 4–20% Invitrogen XP04202BOX Nitrocellulose Membrane, 0.2 μm Bio-Rad 1620112 PBS (10X), pH 7.4 Gibco 70011044 Paraformaldehyde 16% Aqueous Solution EM Grade EMS 15710 TritonTM X-100 Sigma-Aldrich X100-500ML Normal Goat Serum Gibco PCN5000 VECTASHIELD PLUS Antifade Mounting Medium Vector Laboratories H-1900 Methanol, LC-MS Ultra CHROMASOLV Fluka Analytical 14262-1 L Metabolomics Amino Acid Mix Cambridge Isotope Labs MSK-A2-1.2 PierceTM BCA Protein Assay Kits Thermo Scientific 23227 Water suitable for LC/MS, LiChrosolv Supelco 1153331000 Chloroform HPLC Plus Sigma-Aldrich 650471-1 L Schneider’s Drosophila Medium Thermo Scientific 21720024 Experimental models: Organisms/strains D. melanogaster: GFP-RNAi: w[*]; wg[Sp-1]/CyO; P {w[+mC]=UAS-GFP.RNAi.R}142 Bloomington Drosophila Stock Center RRID:BDSC_44415 D. melanogaster: UAS-Cat: w[1]; P{w[+mC]=UASCat.A}2 Bloomington Drosophila Stock Center RRID:BDSC_24621 D. melanogaster: tub-cyto-roGFP2-Orp1: w[1118]; P{w[+mC]=tub-cyto-roGFP2-Orp1}7/TM3, Sb[1] Bloomington Drosophila Stock Center RRID:BDSC_67671 D. melanogaster: Luciferase-RNAi: y[1] v[1]; P{y [+t7.7] v[+t1.8]=TRiP.JF01355}attP2 Bloomington Drosophila Stock Center RRID:BDSC_31603 D. melanogaster: Df(2 R)BSC696: w[1118]; Df(2 R)BSC696, P + PBac{w[+mC]=XP3.WH3} BSC696/CyO Bloomington Drosophila Stock Center RRID:BDSC_26548 (Continued on next page) 12 Cell Reports 45, 117574, July 28, 2026 ..

Liquid Chromatography with Mass Spectroscopy:

Article Title: The pyruvate transporter hermes regulates autophagy and health by modulating ROS production.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Ref(2)p Abcam Cat# ab178440, RRID:AB_2938801 Rabbit polyclonal anti-phospho-Dros-S6K (Thr398) Cell Signaling Technology Cat# 9209, RRID:AB_2269804 Rabbit polyclonal anti-phospho-PDHA1 (S293) Abcam Cat# ab92696, RRID:AB_10711672 Mouse monoclonal anti-Actin DSHB Cat# jla20, RRID:AB_528068 Chemicals, peptides, and recombinant proteins Agar MoorAgar 41004 Brewer’s Yeast Fisher Scientific ICN90331225 Cornmeal Fisher Scientific ICN90141125 Molasses Thomsen Food Services 02625 Propionic acid Fisher Scientific A258 Phosphoric Acid Fisher Scientific A260 Tegosept Genesee 20-259 UltraPureTM SDS Solution, 10% Invitrogen 15553-035 Tris Base Fisher Scientific BP152-1 Ethylenediaminetetraacetic Acid Fisher Scientific BP120-500 HaltTM Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) Thermo Scientific 78445 4× Laemmli Sample Buffer Bio-Rad 1610747 NovexTM Tris-Glycine Mini Protein Gels, 4–20% Invitrogen XP04202BOX Nitrocellulose Membrane, 0.2 μm Bio-Rad 1620112 PBS (10X), pH 7.4 Gibco 70011044 Paraformaldehyde 16% Aqueous Solution EM Grade EMS 15710 TritonTM X-100 Sigma-Aldrich X100-500ML Normal Goat Serum Gibco PCN5000 VECTASHIELD PLUS Antifade Mounting Medium Vector Laboratories H-1900 Methanol, LC-MS Ultra CHROMASOLV Fluka Analytical 14262-1 L Metabolomics Amino Acid Mix Cambridge Isotope Labs MSK-A2-1.2 PierceTM BCA Protein Assay Kits Thermo Scientific 23227 Water suitable for LC/MS, LiChrosolv Supelco 1153331000 Chloroform HPLC Plus Sigma-Aldrich 650471-1 L Schneider’s Drosophila Medium Thermo Scientific 21720024 Experimental models: Organisms/strains D. melanogaster: GFP-RNAi: w[*]; wg[Sp-1]/CyO; P {w[+mC]=UAS-GFP.RNAi.R}142 Bloomington Drosophila Stock Center RRID:BDSC_44415 D. melanogaster: UAS-Cat: w[1]; P{w[+mC]=UASCat.A}2 Bloomington Drosophila Stock Center RRID:BDSC_24621 D. melanogaster: tub-cyto-roGFP2-Orp1: w[1118]; P{w[+mC]=tub-cyto-roGFP2-Orp1}7/TM3, Sb[1] Bloomington Drosophila Stock Center RRID:BDSC_67671 D. melanogaster: Luciferase-RNAi: y[1] v[1]; P{y [+t7.7] v[+t1.8]=TRiP.JF01355}attP2 Bloomington Drosophila Stock Center RRID:BDSC_31603 D. melanogaster: Df(2 R)BSC696: w[1118]; Df(2 R)BSC696, P + PBac{w[+mC]=XP3.WH3} BSC696/CyO Bloomington Drosophila Stock Center RRID:BDSC_26548 (Continued on next page) 12 Cell Reports 45, 117574, July 28, 2026 ..

Article Title: Ecotoxicity of Nitrated Monoaromatic Hydrocarbons in Aquatic Systems: Emerging Risks from Atmospheric Deposition of Biomass Burning and Anthropogenic Aerosols.
Article Snippet: Different dry aerosol extracts, as well as corresponding NMAHs standards, were (re)dissolved in 500 μL of injection solvent and subsequently filtered through 0.22 μm syringe filters (Whatman; GE Healthcare, Little Chalfont, UK). .. The injection solvent consisted of 7.5 mM ammonium formate buffer, pH 3 (made of ammonium formate and formic acid, LC-MS eluent additives, Fluka, Seelze, Germany) and 285 μM EDTA (99.995%). .. The analysis was performed using a triple quadrupole LC/MS-MS (Agilent Technologies, Santa Clara, CA, USA) in conjunction with an UltiMate 3000 UHPLC system (Thermo Scientific, Waltham, MA, USA).

Bicinchoninic Acid Protein Assay:

Article Title: The pyruvate transporter hermes regulates autophagy and health by modulating ROS production.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Ref(2)p Abcam Cat# ab178440, RRID:AB_2938801 Rabbit polyclonal anti-phospho-Dros-S6K (Thr398) Cell Signaling Technology Cat# 9209, RRID:AB_2269804 Rabbit polyclonal anti-phospho-PDHA1 (S293) Abcam Cat# ab92696, RRID:AB_10711672 Mouse monoclonal anti-Actin DSHB Cat# jla20, RRID:AB_528068 Chemicals, peptides, and recombinant proteins Agar MoorAgar 41004 Brewer’s Yeast Fisher Scientific ICN90331225 Cornmeal Fisher Scientific ICN90141125 Molasses Thomsen Food Services 02625 Propionic acid Fisher Scientific A258 Phosphoric Acid Fisher Scientific A260 Tegosept Genesee 20-259 UltraPureTM SDS Solution, 10% Invitrogen 15553-035 Tris Base Fisher Scientific BP152-1 Ethylenediaminetetraacetic Acid Fisher Scientific BP120-500 HaltTM Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) Thermo Scientific 78445 4× Laemmli Sample Buffer Bio-Rad 1610747 NovexTM Tris-Glycine Mini Protein Gels, 4–20% Invitrogen XP04202BOX Nitrocellulose Membrane, 0.2 μm Bio-Rad 1620112 PBS (10X), pH 7.4 Gibco 70011044 Paraformaldehyde 16% Aqueous Solution EM Grade EMS 15710 TritonTM X-100 Sigma-Aldrich X100-500ML Normal Goat Serum Gibco PCN5000 VECTASHIELD PLUS Antifade Mounting Medium Vector Laboratories H-1900 Methanol, LC-MS Ultra CHROMASOLV Fluka Analytical 14262-1 L Metabolomics Amino Acid Mix Cambridge Isotope Labs MSK-A2-1.2 PierceTM BCA Protein Assay Kits Thermo Scientific 23227 Water suitable for LC/MS, LiChrosolv Supelco 1153331000 Chloroform HPLC Plus Sigma-Aldrich 650471-1 L Schneider’s Drosophila Medium Thermo Scientific 21720024 Experimental models: Organisms/strains D. melanogaster: GFP-RNAi: w[*]; wg[Sp-1]/CyO; P {w[+mC]=UAS-GFP.RNAi.R}142 Bloomington Drosophila Stock Center RRID:BDSC_44415 D. melanogaster: UAS-Cat: w[1]; P{w[+mC]=UASCat.A}2 Bloomington Drosophila Stock Center RRID:BDSC_24621 D. melanogaster: tub-cyto-roGFP2-Orp1: w[1118]; P{w[+mC]=tub-cyto-roGFP2-Orp1}7/TM3, Sb[1] Bloomington Drosophila Stock Center RRID:BDSC_67671 D. melanogaster: Luciferase-RNAi: y[1] v[1]; P{y [+t7.7] v[+t1.8]=TRiP.JF01355}attP2 Bloomington Drosophila Stock Center RRID:BDSC_31603 D. melanogaster: Df(2 R)BSC696: w[1118]; Df(2 R)BSC696, P + PBac{w[+mC]=XP3.WH3} BSC696/CyO Bloomington Drosophila Stock Center RRID:BDSC_26548 (Continued on next page) 12 Cell Reports 45, 117574, July 28, 2026 ..

High Performance Liquid Chromatography:

Article Title: The pyruvate transporter hermes regulates autophagy and health by modulating ROS production.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Ref(2)p Abcam Cat# ab178440, RRID:AB_2938801 Rabbit polyclonal anti-phospho-Dros-S6K (Thr398) Cell Signaling Technology Cat# 9209, RRID:AB_2269804 Rabbit polyclonal anti-phospho-PDHA1 (S293) Abcam Cat# ab92696, RRID:AB_10711672 Mouse monoclonal anti-Actin DSHB Cat# jla20, RRID:AB_528068 Chemicals, peptides, and recombinant proteins Agar MoorAgar 41004 Brewer’s Yeast Fisher Scientific ICN90331225 Cornmeal Fisher Scientific ICN90141125 Molasses Thomsen Food Services 02625 Propionic acid Fisher Scientific A258 Phosphoric Acid Fisher Scientific A260 Tegosept Genesee 20-259 UltraPureTM SDS Solution, 10% Invitrogen 15553-035 Tris Base Fisher Scientific BP152-1 Ethylenediaminetetraacetic Acid Fisher Scientific BP120-500 HaltTM Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) Thermo Scientific 78445 4× Laemmli Sample Buffer Bio-Rad 1610747 NovexTM Tris-Glycine Mini Protein Gels, 4–20% Invitrogen XP04202BOX Nitrocellulose Membrane, 0.2 μm Bio-Rad 1620112 PBS (10X), pH 7.4 Gibco 70011044 Paraformaldehyde 16% Aqueous Solution EM Grade EMS 15710 TritonTM X-100 Sigma-Aldrich X100-500ML Normal Goat Serum Gibco PCN5000 VECTASHIELD PLUS Antifade Mounting Medium Vector Laboratories H-1900 Methanol, LC-MS Ultra CHROMASOLV Fluka Analytical 14262-1 L Metabolomics Amino Acid Mix Cambridge Isotope Labs MSK-A2-1.2 PierceTM BCA Protein Assay Kits Thermo Scientific 23227 Water suitable for LC/MS, LiChrosolv Supelco 1153331000 Chloroform HPLC Plus Sigma-Aldrich 650471-1 L Schneider’s Drosophila Medium Thermo Scientific 21720024 Experimental models: Organisms/strains D. melanogaster: GFP-RNAi: w[*]; wg[Sp-1]/CyO; P {w[+mC]=UAS-GFP.RNAi.R}142 Bloomington Drosophila Stock Center RRID:BDSC_44415 D. melanogaster: UAS-Cat: w[1]; P{w[+mC]=UASCat.A}2 Bloomington Drosophila Stock Center RRID:BDSC_24621 D. melanogaster: tub-cyto-roGFP2-Orp1: w[1118]; P{w[+mC]=tub-cyto-roGFP2-Orp1}7/TM3, Sb[1] Bloomington Drosophila Stock Center RRID:BDSC_67671 D. melanogaster: Luciferase-RNAi: y[1] v[1]; P{y [+t7.7] v[+t1.8]=TRiP.JF01355}attP2 Bloomington Drosophila Stock Center RRID:BDSC_31603 D. melanogaster: Df(2 R)BSC696: w[1118]; Df(2 R)BSC696, P + PBac{w[+mC]=XP3.WH3} BSC696/CyO Bloomington Drosophila Stock Center RRID:BDSC_26548 (Continued on next page) 12 Cell Reports 45, 117574, July 28, 2026 ..

Luciferase:

Article Title: The pyruvate transporter hermes regulates autophagy and health by modulating ROS production.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-Ref(2)p Abcam Cat# ab178440, RRID:AB_2938801 Rabbit polyclonal anti-phospho-Dros-S6K (Thr398) Cell Signaling Technology Cat# 9209, RRID:AB_2269804 Rabbit polyclonal anti-phospho-PDHA1 (S293) Abcam Cat# ab92696, RRID:AB_10711672 Mouse monoclonal anti-Actin DSHB Cat# jla20, RRID:AB_528068 Chemicals, peptides, and recombinant proteins Agar MoorAgar 41004 Brewer’s Yeast Fisher Scientific ICN90331225 Cornmeal Fisher Scientific ICN90141125 Molasses Thomsen Food Services 02625 Propionic acid Fisher Scientific A258 Phosphoric Acid Fisher Scientific A260 Tegosept Genesee 20-259 UltraPureTM SDS Solution, 10% Invitrogen 15553-035 Tris Base Fisher Scientific BP152-1 Ethylenediaminetetraacetic Acid Fisher Scientific BP120-500 HaltTM Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) Thermo Scientific 78445 4× Laemmli Sample Buffer Bio-Rad 1610747 NovexTM Tris-Glycine Mini Protein Gels, 4–20% Invitrogen XP04202BOX Nitrocellulose Membrane, 0.2 μm Bio-Rad 1620112 PBS (10X), pH 7.4 Gibco 70011044 Paraformaldehyde 16% Aqueous Solution EM Grade EMS 15710 TritonTM X-100 Sigma-Aldrich X100-500ML Normal Goat Serum Gibco PCN5000 VECTASHIELD PLUS Antifade Mounting Medium Vector Laboratories H-1900 Methanol, LC-MS Ultra CHROMASOLV Fluka Analytical 14262-1 L Metabolomics Amino Acid Mix Cambridge Isotope Labs MSK-A2-1.2 PierceTM BCA Protein Assay Kits Thermo Scientific 23227 Water suitable for LC/MS, LiChrosolv Supelco 1153331000 Chloroform HPLC Plus Sigma-Aldrich 650471-1 L Schneider’s Drosophila Medium Thermo Scientific 21720024 Experimental models: Organisms/strains D. melanogaster: GFP-RNAi: w[*]; wg[Sp-1]/CyO; P {w[+mC]=UAS-GFP.RNAi.R}142 Bloomington Drosophila Stock Center RRID:BDSC_44415 D. melanogaster: UAS-Cat: w[1]; P{w[+mC]=UASCat.A}2 Bloomington Drosophila Stock Center RRID:BDSC_24621 D. melanogaster: tub-cyto-roGFP2-Orp1: w[1118]; P{w[+mC]=tub-cyto-roGFP2-Orp1}7/TM3, Sb[1] Bloomington Drosophila Stock Center RRID:BDSC_67671 D. melanogaster: Luciferase-RNAi: y[1] v[1]; P{y [+t7.7] v[+t1.8]=TRiP.JF01355}attP2 Bloomington Drosophila Stock Center RRID:BDSC_31603 D. melanogaster: Df(2 R)BSC696: w[1118]; Df(2 R)BSC696, P + PBac{w[+mC]=XP3.WH3} BSC696/CyO Bloomington Drosophila Stock Center RRID:BDSC_26548 (Continued on next page) 12 Cell Reports 45, 117574, July 28, 2026 ..

Injection:

Article Title: Ecotoxicity of Nitrated Monoaromatic Hydrocarbons in Aquatic Systems: Emerging Risks from Atmospheric Deposition of Biomass Burning and Anthropogenic Aerosols.
Article Snippet: Different dry aerosol extracts, as well as corresponding NMAHs standards, were (re)dissolved in 500 μL of injection solvent and subsequently filtered through 0.22 μm syringe filters (Whatman; GE Healthcare, Little Chalfont, UK). .. The injection solvent consisted of 7.5 mM ammonium formate buffer, pH 3 (made of ammonium formate and formic acid, LC-MS eluent additives, Fluka, Seelze, Germany) and 285 μM EDTA (99.995%). .. The analysis was performed using a triple quadrupole LC/MS-MS (Agilent Technologies, Santa Clara, CA, USA) in conjunction with an UltiMate 3000 UHPLC system (Thermo Scientific, Waltham, MA, USA).

Solvent:

Article Title: Ecotoxicity of Nitrated Monoaromatic Hydrocarbons in Aquatic Systems: Emerging Risks from Atmospheric Deposition of Biomass Burning and Anthropogenic Aerosols.
Article Snippet: Different dry aerosol extracts, as well as corresponding NMAHs standards, were (re)dissolved in 500 μL of injection solvent and subsequently filtered through 0.22 μm syringe filters (Whatman; GE Healthcare, Little Chalfont, UK). .. The injection solvent consisted of 7.5 mM ammonium formate buffer, pH 3 (made of ammonium formate and formic acid, LC-MS eluent additives, Fluka, Seelze, Germany) and 285 μM EDTA (99.995%). .. The analysis was performed using a triple quadrupole LC/MS-MS (Agilent Technologies, Santa Clara, CA, USA) in conjunction with an UltiMate 3000 UHPLC system (Thermo Scientific, Waltham, MA, USA).

other:

Article Title: Environmental Impact of Fluoroquinolones and Their Photocatalytic Transformation Products: Degradation with Activated Sludge and in Surface Waters, Change in Antimicrobial Activity and Ecotoxicity.
Article Snippet: Furthermore, NaOH (purity p.a., Chempur, Piekary Śląskie, Poland), water for LC-MS (Chromasolv®; Fluka-Analytical, Buchs, Switzerland), acetonitrile for LC-MS (LiChrosolv®; Supelco, Bellefonte, PA, USA), formic acid (98–100% for LC-MS, LiChropur®; Supelco, Bellefonte, PA, USA) and leucine enkephalin (Waters, Milford, MA, USA) were used in experiments.



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Roche magna pure lc automated extraction system
Reaction schemes <t>and</t> <t>LC-MS</t> analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.
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Reaction schemes <t>and</t> <t>LC-MS</t> analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.
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Fisher Scientific lc grade acetonitrile
Reaction schemes <t>and</t> <t>LC-MS</t> analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.
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JASCO Inc lc 4000 uhplc 310
Reaction schemes <t>and</t> <t>LC-MS</t> analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.
Lc 4000 Uhplc 310, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Reaction schemes <t>and</t> <t>LC-MS</t> analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.
Jasco X Lc Hplc System, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Reaction schemes <t>and</t> <t>LC-MS</t> analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.
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(A) Volcano plot of untargeted metabolomic changes in PAH enu2 brains relative to WT, showing increased Phe-related metabolites and decreased metabolites associated with tyrosine metabolism, redox balance, and central carbon metabolism. (B) Volcano plot of label-free proteomic changes in PKU brains relative to WT, highlighting significant alterations in proteins related to proteostasis, translation, neuronal signaling, and stress adaptive responses. (C) Z-score matrix showing pathway-level activity. Metabolomic and proteomic contributions are presented separately, highlighting specific alterations in redox and detoxification, proteostasis, the TCA cycle, and lipid-related pathways. (D) Integrated cell-fate matrix combining metabolomic and proteomic evidence into a single activity state. The Phe axis was the most strongly activated integrated module, whereas myelin glial remodeling was suppressed. Redox oxidative stress showed a mixed integrated pattern, consistent with pathway remodeling rather than uniform activation or suppression. (E) <t>HPLC</t> quantification of reduced glutathione (GSH), cysteine (CYS), and ATP levels in WT and PKU brain tissue. Data are expressed as mean ± SD normalized to mg of cortex (* < 0.05) (n=6/group).
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(A) Volcano plot of untargeted metabolomic changes in PAH enu2 brains relative to WT, showing increased Phe-related metabolites and decreased metabolites associated with tyrosine metabolism, redox balance, and central carbon metabolism. (B) Volcano plot of label-free proteomic changes in PKU brains relative to WT, highlighting significant alterations in proteins related to proteostasis, translation, neuronal signaling, and stress adaptive responses. (C) Z-score matrix showing pathway-level activity. Metabolomic and proteomic contributions are presented separately, highlighting specific alterations in redox and detoxification, proteostasis, the TCA cycle, and lipid-related pathways. (D) Integrated cell-fate matrix combining metabolomic and proteomic evidence into a single activity state. The Phe axis was the most strongly activated integrated module, whereas myelin glial remodeling was suppressed. Redox oxidative stress showed a mixed integrated pattern, consistent with pathway remodeling rather than uniform activation or suppression. (E) <t>HPLC</t> quantification of reduced glutathione (GSH), cysteine (CYS), and ATP levels in WT and PKU brain tissue. Data are expressed as mean ± SD normalized to mg of cortex (* < 0.05) (n=6/group).
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Reaction schemes and LC-MS analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.

Journal: Synthetic and Systems Biotechnology

Article Title: Functional characterization of four glycosyltransferases for biosynthesis of steroidal saponins in medicinal plant Paris polyphylla

doi: 10.1016/j.synbio.2026.04.002

Figure Lengend Snippet: Reaction schemes and LC-MS analysis of Pp UGTs reactions with diosgenin and pennogenin . ( a ) Biosynthesis of trillin mediated by UGT91BP2 and UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of diosgenin. ( b ) Extracted ion chromatograms (EICs) of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and diosgenin showing the formation of trillin, as compared to the authentic trillin standard. Control is the empty expression vector. ( c ) MS spectra of the enzymatic reaction products in hydrogen and sodium ion adducts compared to the fragmentation pattern of the trillin standard. The product molecular ions [M + H] + and [M +Na] + and the feature fragment ion [M + H − Glc] + with m / z 415.32 are marked. Of note, additional peaks observed in the diosgenin EIC ( m / z 415.32) for UGT703R1–3 might correspond to substrate isomeric impurities, in-source adducts, or minor non-enzymatic by-products; these peaks, which arose from the extracted ion flow of the substrate m / z , did not co-elute with the product, trillin , and therefore did not affect the interpretation of the product formation. ( d ) Biosynthesis of pennogenin 3- O -glucoside mediated by the UGT703R1–3 through transferring glucose from UDP-glucose to the C-3 OH position of pennogenin. ( e ) EICs of the in vitro enzyme activity assays of recombinant UGT91BP2 and UGT703R1–3 with UDP-glucose and pennogenin showing the formation of pennogenin 3- O -glucoside, compared to the control empty expression vector. ( f ) MS/MS spectra ([M + H – Glc] + and [M + H – Glc − H 2 O] + ) of the enzymatic reaction products.

Article Snippet: Liquid chromatography-mass spectrometry (LC-MS) was equipped with a YMC-Triart C18 column (250 mm × 4.6 mm, 5 μm), and the column temperature was set to 30 °C.

Techniques: Liquid Chromatography with Mass Spectroscopy, Transferring, In Vitro, Activity Assay, Recombinant, Control, Expressing, Plasmid Preparation, Tandem Mass Spectroscopy

(A) Volcano plot of untargeted metabolomic changes in PAH enu2 brains relative to WT, showing increased Phe-related metabolites and decreased metabolites associated with tyrosine metabolism, redox balance, and central carbon metabolism. (B) Volcano plot of label-free proteomic changes in PKU brains relative to WT, highlighting significant alterations in proteins related to proteostasis, translation, neuronal signaling, and stress adaptive responses. (C) Z-score matrix showing pathway-level activity. Metabolomic and proteomic contributions are presented separately, highlighting specific alterations in redox and detoxification, proteostasis, the TCA cycle, and lipid-related pathways. (D) Integrated cell-fate matrix combining metabolomic and proteomic evidence into a single activity state. The Phe axis was the most strongly activated integrated module, whereas myelin glial remodeling was suppressed. Redox oxidative stress showed a mixed integrated pattern, consistent with pathway remodeling rather than uniform activation or suppression. (E) HPLC quantification of reduced glutathione (GSH), cysteine (CYS), and ATP levels in WT and PKU brain tissue. Data are expressed as mean ± SD normalized to mg of cortex (* < 0.05) (n=6/group).

Journal: bioRxiv

Article Title: Integrated metabolic and proteostatic profiling reveals remodeling of proteolytic pathways associated with redox-bioenergetic dysfunction in a PAH enu2 mouse model of phenylketonuria

doi: 10.64898/2026.07.08.736353

Figure Lengend Snippet: (A) Volcano plot of untargeted metabolomic changes in PAH enu2 brains relative to WT, showing increased Phe-related metabolites and decreased metabolites associated with tyrosine metabolism, redox balance, and central carbon metabolism. (B) Volcano plot of label-free proteomic changes in PKU brains relative to WT, highlighting significant alterations in proteins related to proteostasis, translation, neuronal signaling, and stress adaptive responses. (C) Z-score matrix showing pathway-level activity. Metabolomic and proteomic contributions are presented separately, highlighting specific alterations in redox and detoxification, proteostasis, the TCA cycle, and lipid-related pathways. (D) Integrated cell-fate matrix combining metabolomic and proteomic evidence into a single activity state. The Phe axis was the most strongly activated integrated module, whereas myelin glial remodeling was suppressed. Redox oxidative stress showed a mixed integrated pattern, consistent with pathway remodeling rather than uniform activation or suppression. (E) HPLC quantification of reduced glutathione (GSH), cysteine (CYS), and ATP levels in WT and PKU brain tissue. Data are expressed as mean ± SD normalized to mg of cortex (* < 0.05) (n=6/group).

Article Snippet: Detection was performed at 254 nm using a Jasco HPLC LC-net II (Jasco Inc., Japan).

Techniques: Metabolomic, Activity Assay, Activation Assay

HPLC quantification of (A) ATP, ADP, AMP, NAD, and NADH levels from WT and PKU mice; (B) reduced glutathione (GSH) and cysteine contents in whole blood (n=6/group). (C) Activities of glutathione peroxidase (GPX), glutathione reductase (GR), and glucose-6-phosphate dehydrogenase (G6PD) measured in blood hemolysates (n=9/group). Data are expressed as mean ± SD normalized on hemoglobin (Hb) values (* < 0.05; ** < 0.01).

Journal: bioRxiv

Article Title: Integrated metabolic and proteostatic profiling reveals remodeling of proteolytic pathways associated with redox-bioenergetic dysfunction in a PAH enu2 mouse model of phenylketonuria

doi: 10.64898/2026.07.08.736353

Figure Lengend Snippet: HPLC quantification of (A) ATP, ADP, AMP, NAD, and NADH levels from WT and PKU mice; (B) reduced glutathione (GSH) and cysteine contents in whole blood (n=6/group). (C) Activities of glutathione peroxidase (GPX), glutathione reductase (GR), and glucose-6-phosphate dehydrogenase (G6PD) measured in blood hemolysates (n=9/group). Data are expressed as mean ± SD normalized on hemoglobin (Hb) values (* < 0.05; ** < 0.01).

Article Snippet: Detection was performed at 254 nm using a Jasco HPLC LC-net II (Jasco Inc., Japan).

Techniques: