laser scanning densitometry (Pharmacia LKB Biotechnology Inc)
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Laser Scanning Densitometry, supplied by Pharmacia LKB Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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other:Article Title: Thyrotropin receptor and leukocyte adhesion molecules in autoimmune thyroid disease: a study of their gene expression by northern blot analysis and in situ hybridization. Article Snippet: In order to characterize the role of leukocyte-activating antigens and other immunological parameters in autoimmune thyroid disease, mRNA levels of intercellular adhesion molecule 1 (ICAM-1), endothelial leukocyte adhesion molecule 1 (ELAM-1, E-selectin), invariant chain (Ii) and the thymic hormone thymosin \g=b\4 (T\g=b\4) were investigated in 18 human thyroid glands, including eight Graves' thyroids, two Hashimoto's thyroids, two endemic goiters and six healthy controls.. Northern blot analysis showed that in autoimmune thyroid disease, expression of ICAM-1 and T\g=b\4 was correlated to transcript levels of Ii, whereas in the healthy controls, expression of T\g=b\4, ICAM-1 and ELAM-1 was low Article Title: Increased major histocompatibility complex (MHC) expression in nontoxic goiters is associated with iodide depletion, enhanced ability of the follicular thyroglobulin to increase MHC gene expression, and thyroid autoantibodies. Article Snippet: Autoradiographic signals were quantified by Immunopeptidomics:Article Title: TSH-receptor and adhesion molecules in autoimmune thyroid disease. Article Snippet: Zusammenfassung: Mittels Northern-Blot-Analyse und einer menschlichen TSH-Rezeptor-(TSH-R-)cDNA-Sonde untersuchten wir das Niveau der TSH-R-Expression in 20 menschlichen Schilddrüsenfragmenten: 14 Basedow-Erkrankungn, 2 Hashimoto-Erkrankungen, 3 endemischen Strumen und eine gesunde Schilddrüse.. Die TSH-R-Expression war niedrig in Schilddrüsen mit hohem Expressionsniveau des Haupthistokompatibilitätskomplex Klasse I (MHC I), Klasse II (MHC Il) und des interzellulären Adhäsionsmoleküls 1 (ICAM1), wohingegen die Expression des endothelialen Leukozyten Adhäsionsmoleküls I (ELAM-1) in keiner erkennbaren Verbindung zur TSHR-Expression stand.. In-situ-Hybridisierung zeigte, daß neben den Lymphozyten (MHC II) Schilddrüsenzellen (MHC 11, ICAM-1) und Endothelzellen (ICAM-1, ELAM-1) die Quelle für die Transkripte dieser T-ZelI-aktivierenden Antigene waren. Quantitation Assay:Article Title: Effects of gonadal and adrenal androgens in a novel androgen-responsive human osteoblastic cell line Article Snippet: While androgens have important skeletal effects, the mechanism(s) of androgen action on bone remain unclear.. Current osteoblast models to study androgen effects have several limitations, including the presence of heterogeneous cell populations.. In this study, we examined the effects of androgens on the proliferation and differentiation of a novel human fetal osteoblastic cell line (hFOB/AR-6) that expresses a mature osteoblast phenotype and a physiological number (,4,000/nucleus) of androgen receptors (AR). |
