lambda phage packaging extracts maxplaxtm (Lucigen Corp)
90
Structured Review
Lucigen Corp
lambda phage packaging extracts maxplaxtm
Lambda Phage Packaging Extracts Maxplaxtm, supplied by Lucigen Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda-phage+packaging+extracts/maxplax+lambda+packaging+extracts/pm36212884-76-6-11
Average 90 stars, based on 1 article reviews
Lambda Phage Packaging Extracts Maxplaxtm, supplied by Lucigen Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda-phage+packaging+extracts/maxplax+lambda+packaging+extracts/pm36212884-76-6-11
Average 90 stars, based on 1 article reviews
lambda phage packaging extracts maxplaxtm - by Bioz Stars,
2026-09
90/100 stars
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Construct:Article Title: Precision Discovery of Novel Inhibitors of Human Cancer Target HsMetAP 1 from Vast Unexplored Metagenomic Diversity Article Snippet: .. Cosmid libraries were constructed using Clone Assay:Article Title: Precision Discovery of Novel Inhibitors of Human Cancer Target HsMetAP 1 from Vast Unexplored Metagenomic Diversity Article Snippet: .. Cosmid libraries were constructed using Article Title: Metagenomic library and natural product discovery platform Article Snippet: Approximately 10 ligation reactions containing 250 ng of blunt-ended DNA was combined with 500 ng of a blunt-ended cosmid vector (T4 ligase, NEB, M0202) and cloned into a cosmid backbone. .. The cloned DNA was packaged into phages and transduced into E. coli using a Ligation:Article Title: High-throughput retrieval of target sequences from complex clone libraries using CRISPRi Article Snippet: A 5 μL ligation reaction with 125 ng blunted insert DNA and 250 ng vector using the Fast-link ligation kit (Lucigen) was prepared and incubated overnight at ~22 °C. .. The ligation was then used for lambda phage packaging with Article Title: High-throughput retrieval of target sequences from complex clone libraries using CRISPRi. Article Snippet: The capture of metagenomic DNA in large clone libraries provides the opportunity to study microbial diversity that is inaccessible using culture-dependent methods.. In this study, we harnessed nuclease-deficient Cas9 to establish a CRISPR counter-selection interruption circuit (CCIC) that can be used to retrieve target clones from complex libraries.. Combining modern sequencing methods with CCIC cloning allows for rapid physical access to the genetic diversity present in natural ecosystems. Article Title: Isolation, identification, and biochemical characterization of a novel bifunctional phosphomannomutase/phosphoglucomutase from the metagenome of the brown alga Laminaria digitata . Article Snippet: A ligation reaction was performed with ~0.25 μg insert (20–50 Kb mDNA) and ~0.5 μg linearized vector T4 ligase (New England Biolabs, United Kingdom). .. Ligation reaction products were packaged in other:Article Title: Multiplexed functional metagenomic analysis of the infant microbiome identifies effectors of NF-κB, autophagy, and cellular redox state Article Snippet: Purified high-molecular-weight DNA was blunt-ended (End-It Article Title: Metagenomic library and natural product discovery platform Article Snippet: Approximately 10 ligation reactions containing 250 ng of blunt-ended DNA was combined with 500 ng of a blunt-ended cosmid vector (T4 ligase, NEB, M0202) and cloned into a cosmid backbone. Transfection:Article Title: Isolation, identification, and biochemical characterization of a novel bifunctional phosphomannomutase/phosphoglucomutase from the metagenome of the brown alga Laminaria digitata . Article Snippet: A ligation reaction was performed with ~0.25 μg insert (20–50 Kb mDNA) and ~0.5 μg linearized vector T4 ligase (New England Biolabs, United Kingdom). .. Ligation reaction products were packaged in Knock-Out:Article Title: MadR mediates acyl CoA-dependent regulation of mycolic acid desaturation in mycobacteria Article Snippet: .. The resultant phasmid was packaged with |