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lambda zapii λ phage vector  (Agilent technologies)


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    Agilent technologies lambda zapii λ phage vector
    Pep1 hybridization of a 2-kb AccI genomic segment of B. caccae. Three clones (5-2, 3-2, and 1-1) were isolated from a primary screen of a genomic B. caccae <t>λ</t> <t>phage</t> library. The clones were digested with AccI, electrophoresed, transferred to nitrocellulose membranes, and hybridized with the Pep1 or Pep2 oligonucleotide probe. (Left panel) Ethidium bromide-stained gel; (middle panel) membrane hybridization with Pep1 oligonucleotide; (right panel) membrane hybridization with Pep2 oligonucleotide.
    Lambda Zapii λ Phage Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+zapii+phage+vector/pmc00098733-154-12-17
    Average 90 stars, based on 1 article reviews
    lambda zapii λ phage vector - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Molecular Cloning of a Bacteroides caccae TonB-Linked Outer Membrane Protein Identified by an Inflammatory Bowel Disease Marker Antibody"

    Article Title: Molecular Cloning of a Bacteroides caccae TonB-Linked Outer Membrane Protein Identified by an Inflammatory Bowel Disease Marker Antibody

    Journal:

    doi: 10.1128/IAI.69.10.6044-6054.2001

    Pep1 hybridization of a 2-kb AccI genomic segment of B. caccae. Three clones (5-2, 3-2, and 1-1) were isolated from a primary screen of a genomic B. caccae λ phage library. The clones were digested with AccI, electrophoresed, transferred to nitrocellulose membranes, and hybridized with the Pep1 or Pep2 oligonucleotide probe. (Left panel) Ethidium bromide-stained gel; (middle panel) membrane hybridization with Pep1 oligonucleotide; (right panel) membrane hybridization with Pep2 oligonucleotide.
    Figure Legend Snippet: Pep1 hybridization of a 2-kb AccI genomic segment of B. caccae. Three clones (5-2, 3-2, and 1-1) were isolated from a primary screen of a genomic B. caccae λ phage library. The clones were digested with AccI, electrophoresed, transferred to nitrocellulose membranes, and hybridized with the Pep1 or Pep2 oligonucleotide probe. (Left panel) Ethidium bromide-stained gel; (middle panel) membrane hybridization with Pep1 oligonucleotide; (right panel) membrane hybridization with Pep2 oligonucleotide.

    Techniques Used: Hybridization, Clone Assay, Isolation, Staining

    Cloning of the ompW gene. A genomic λ phage library of p2Lc3 was constructed and screened using Pep1 and Pep2 probes. Southern analysis identified an authentic Pep1-positive genomic clone, designated 5-2 (Fig. ​(Fig.1),1), which contained an artifactual insert Tn10 sequence. The native ompW gene was isolated by genome walking using p2Lc3 genomic DNA as substrate. Sequence analysis of a 4,043-bp fragment revealed an open reading frame encoding 947 amino acids and an upstream AT-rich putative promoter locus.
    Figure Legend Snippet: Cloning of the ompW gene. A genomic λ phage library of p2Lc3 was constructed and screened using Pep1 and Pep2 probes. Southern analysis identified an authentic Pep1-positive genomic clone, designated 5-2 (Fig. ​(Fig.1),1), which contained an artifactual insert Tn10 sequence. The native ompW gene was isolated by genome walking using p2Lc3 genomic DNA as substrate. Sequence analysis of a 4,043-bp fragment revealed an open reading frame encoding 947 amino acids and an upstream AT-rich putative promoter locus.

    Techniques Used: Clone Assay, Construct, Sequencing, Isolation

    Related Articles

    Clone Assay:

    Article Title: The Gfi-1B Proto-Oncoprotein Represses p21 WAF1 and Inhibits Myeloid Cell Differentiation
    Article Snippet: .. A 2.9-kb EcoRI fragment from rat genomic DNA hybridizing to a DNA probe from the zinc finger region of the rat Gfi-1 gene (40% formamide, 63 SSC [13 SSC is 0.15 M NaCl plus 0.015 M sodium citrate] at 37°C) was cloned into the lambda phage vector lambda ZapII (Stratagene). ..

    Plasmid Preparation:

    Article Title: The Gfi-1B Proto-Oncoprotein Represses p21 WAF1 and Inhibits Myeloid Cell Differentiation
    Article Snippet: .. A 2.9-kb EcoRI fragment from rat genomic DNA hybridizing to a DNA probe from the zinc finger region of the rat Gfi-1 gene (40% formamide, 63 SSC [13 SSC is 0.15 M NaCl plus 0.015 M sodium citrate] at 37°C) was cloned into the lambda phage vector lambda ZapII (Stratagene). ..

    Article Title: Annotation of expressed sequence tags for the East African cichlid fish Astatotilapia burtoni and evolutionary analyses of cichlid ORFs
    Article Snippet: .. A full-length, directional (EcoRI – XhoI) cDNA library was constructed in Lambda ZapII phage vector (Stratagene) with mRNA from A. burtoni brains (both sexes at all stages of development and social condition were included). .. A full-length, directional (EcoRI – XhoI) cDNA library was constructed in Lambda ZapII phage vector (Stratagene) with mRNA from A. burtoni brains (both sexes at all stages of development and social condition were included).

    Article Title: Molecular Cloning of a Bacteroides caccae TonB-Linked Outer Membrane Protein Identified by an Inflammatory Bowel Disease Marker Antibody
    Article Snippet: Alkaline phosphatase-conjugated goat anti-human F(ab) 2 (Pierce, Rockford, Ill.) was used as the secondary antibody in Western blot analysis. .. Genomic library of a B. caccae clinical isolate (p2Lc3) was constructed using lambda ZAPII λ phage vector (Stratagene, La Jolla, Calif.). .. Briefly, chromosomal DNA was purified from p2Lc3 using the ASAP genomic DNA isolation kit (Boehringer Mannheim, Indianapolis, Ind.), partially digested with Eco RI, and fragments ranging from 1.5 to 9 kb were ligated into lambda ZAPII Eco RI-digested, calf intestinal alkaline phosphatase (CIAP)-treated vector.

    cDNA Library Assay:

    Article Title: Annotation of expressed sequence tags for the East African cichlid fish Astatotilapia burtoni and evolutionary analyses of cichlid ORFs
    Article Snippet: .. A full-length, directional (EcoRI – XhoI) cDNA library was constructed in Lambda ZapII phage vector (Stratagene) with mRNA from A. burtoni brains (both sexes at all stages of development and social condition were included). .. A full-length, directional (EcoRI – XhoI) cDNA library was constructed in Lambda ZapII phage vector (Stratagene) with mRNA from A. burtoni brains (both sexes at all stages of development and social condition were included).

    Construct:

    Article Title: Annotation of expressed sequence tags for the East African cichlid fish Astatotilapia burtoni and evolutionary analyses of cichlid ORFs
    Article Snippet: .. A full-length, directional (EcoRI – XhoI) cDNA library was constructed in Lambda ZapII phage vector (Stratagene) with mRNA from A. burtoni brains (both sexes at all stages of development and social condition were included). .. A full-length, directional (EcoRI – XhoI) cDNA library was constructed in Lambda ZapII phage vector (Stratagene) with mRNA from A. burtoni brains (both sexes at all stages of development and social condition were included).

    Article Title: Molecular Cloning of a Bacteroides caccae TonB-Linked Outer Membrane Protein Identified by an Inflammatory Bowel Disease Marker Antibody
    Article Snippet: Alkaline phosphatase-conjugated goat anti-human F(ab) 2 (Pierce, Rockford, Ill.) was used as the secondary antibody in Western blot analysis. .. Genomic library of a B. caccae clinical isolate (p2Lc3) was constructed using lambda ZAPII λ phage vector (Stratagene, La Jolla, Calif.). .. Briefly, chromosomal DNA was purified from p2Lc3 using the ASAP genomic DNA isolation kit (Boehringer Mannheim, Indianapolis, Ind.), partially digested with Eco RI, and fragments ranging from 1.5 to 9 kb were ligated into lambda ZAPII Eco RI-digested, calf intestinal alkaline phosphatase (CIAP)-treated vector.



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    lambda zapii phage vector  (Agilent technologies)
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    lambda zapii λ phage vector  (Agilent technologies)
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    Agilent technologies lambda zapii λ phage vector
    Pep1 hybridization of a 2-kb AccI genomic segment of B. caccae. Three clones (5-2, 3-2, and 1-1) were isolated from a primary screen of a genomic B. caccae <t>λ</t> <t>phage</t> library. The clones were digested with AccI, electrophoresed, transferred to nitrocellulose membranes, and hybridized with the Pep1 or Pep2 oligonucleotide probe. (Left panel) Ethidium bromide-stained gel; (middle panel) membrane hybridization with Pep1 oligonucleotide; (right panel) membrane hybridization with Pep2 oligonucleotide.
    Lambda Zapii λ Phage Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+zapii+phage+vector/pmc00098733-154-12-17
    Average 90 stars, based on 1 article reviews
    lambda zapii λ phage vector - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Pep1 hybridization of a 2-kb AccI genomic segment of B. caccae. Three clones (5-2, 3-2, and 1-1) were isolated from a primary screen of a genomic B. caccae λ phage library. The clones were digested with AccI, electrophoresed, transferred to nitrocellulose membranes, and hybridized with the Pep1 or Pep2 oligonucleotide probe. (Left panel) Ethidium bromide-stained gel; (middle panel) membrane hybridization with Pep1 oligonucleotide; (right panel) membrane hybridization with Pep2 oligonucleotide.

    Journal:

    Article Title: Molecular Cloning of a Bacteroides caccae TonB-Linked Outer Membrane Protein Identified by an Inflammatory Bowel Disease Marker Antibody

    doi: 10.1128/IAI.69.10.6044-6054.2001

    Figure Lengend Snippet: Pep1 hybridization of a 2-kb AccI genomic segment of B. caccae. Three clones (5-2, 3-2, and 1-1) were isolated from a primary screen of a genomic B. caccae λ phage library. The clones were digested with AccI, electrophoresed, transferred to nitrocellulose membranes, and hybridized with the Pep1 or Pep2 oligonucleotide probe. (Left panel) Ethidium bromide-stained gel; (middle panel) membrane hybridization with Pep1 oligonucleotide; (right panel) membrane hybridization with Pep2 oligonucleotide.

    Article Snippet: Genomic library of a B. caccae clinical isolate (p2Lc3) was constructed using lambda ZAPII λ phage vector (Stratagene, La Jolla, Calif.).

    Techniques: Hybridization, Clone Assay, Isolation, Staining

    Cloning of the ompW gene. A genomic λ phage library of p2Lc3 was constructed and screened using Pep1 and Pep2 probes. Southern analysis identified an authentic Pep1-positive genomic clone, designated 5-2 (Fig. ​(Fig.1),1), which contained an artifactual insert Tn10 sequence. The native ompW gene was isolated by genome walking using p2Lc3 genomic DNA as substrate. Sequence analysis of a 4,043-bp fragment revealed an open reading frame encoding 947 amino acids and an upstream AT-rich putative promoter locus.

    Journal:

    Article Title: Molecular Cloning of a Bacteroides caccae TonB-Linked Outer Membrane Protein Identified by an Inflammatory Bowel Disease Marker Antibody

    doi: 10.1128/IAI.69.10.6044-6054.2001

    Figure Lengend Snippet: Cloning of the ompW gene. A genomic λ phage library of p2Lc3 was constructed and screened using Pep1 and Pep2 probes. Southern analysis identified an authentic Pep1-positive genomic clone, designated 5-2 (Fig. ​(Fig.1),1), which contained an artifactual insert Tn10 sequence. The native ompW gene was isolated by genome walking using p2Lc3 genomic DNA as substrate. Sequence analysis of a 4,043-bp fragment revealed an open reading frame encoding 947 amino acids and an upstream AT-rich putative promoter locus.

    Article Snippet: Genomic library of a B. caccae clinical isolate (p2Lc3) was constructed using lambda ZAPII λ phage vector (Stratagene, La Jolla, Calif.).

    Techniques: Clone Assay, Construct, Sequencing, Isolation