Review



goat serum  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Proteintech goat serum
    Goat Serum, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+protein+phosphatase+buffer/PKC+iota+Antibody/pmc10812620-82-28-42
    Average 93 stars, based on 13 article reviews
    goat serum - by Bioz Stars, 2026-11
    93/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Polarity Gene PARD6B Promotes Tumor Growth of Colorectal Cancer via Increasing MYC Expression
    Article Snippet: .. Cells were lysed in IP buffer, and lysates were incubated overnight at 4°C with anti‐PKC iota antibody (13883‐1‐AP, 1 μg/500 μg protein, Proteintech). .. Complexes were eluted using a Capturem IP & Co‐IP kit (Cat. No. 635721, TaKaRa Bio, Japan) and analyzed by western blotting.

    other:

    Article Title: Prkci promotes colorectal cancer metastasis by phosphorylating and stabilizing Tgfbr1 to activate TGF-β signaling
    Article Snippet: Antibodies for Prkci (66493, proteintech), β-actin (ab8226, abcam), pan Phospho-Serine/Threonine (AP1475, Abclonal), HA-tag (66006, proteintech), His-tag (66005, proteintech), Flag-tag (66008, proteintech), Tgfbr1 (30117, proteintech), Tgfbr2 (66636, proteintech), p-Smad2 (80427, proteintech), Smad2 (67343, proteintech), p-Smad3 (ab63403, Abcam), Anti-Ubiquitin K11 linkage Antibody (2A3/2E6, sigma), Smad3 (ab208182, Abcam), and Smad4 (ab40759, Abcam) were bought from identified companies.

    Article Title: Prkci promotes colorectal cancer metastasis by phosphorylating and stabilizing Tgfbr1 to activate TGF-β signaling.
    Article Snippet: Antibodies for Prkci (66493, proteintech), β-actin (ab8226, abcam), pan Phospho-Serine/Threonine (AP1475, Abclonal), HA-tag (66006, proteintech), Histag (66005, proteintech), Flag-tag (66008, proteintech), Tgfbr1 (30117, proteintech), Tgfbr2 (66636, proteintech), p-Smad2 (80427, proteintech), Smad2 (67343, proteintech), p-Smad3 (ab63403, Abcam), Anti-Ubiquitin K11 linkage Antibody (2A3/2E6, sigma), Smad3 (ab208182, Abcam), and Smad4 (ab40759, Abcam) were bought from identified companies.

    Article Title: Prkci acts a pro-proliferation factor in colorectal cancer
    Article Snippet: Antibodies for Prkci (66493, Proteintech), Gapdh (ab181602, Abcam), Cdk4 (ab108357, Abcam), Glut1 (66290, Proteintech), HA-tag (66006, Proteintech), His-tag (66005, Proteintech), Flag-tag (66008, Proteintech), and pan Phospho-Serine/Threonine (AP1475, Abclonal) were purchased from commercial companies.

    Article Title: Prkci activates Jak2/Stat3 signaling to promote tumor angiogenesis
    Article Snippet: Antibodies for Prkci (66493, proteintech), β-actin (ab8226, abcam), Vegfa (ab52917, abcam), p-Jak2 (ab32101, abcam), Jak2 (ab108596, abcam), p-Stat3 (ab267373, abcam), Stat3 (ab68153, abcam), Na+/K+-ATPase (A11683, ABclonal), Gapdh (ab181602, abcam), pan Phospho-Serine/Threonine (AP1475, Abclonal), and CD31 (A19014, Abclonal) were bought from identified companies.

    Knock-Out:

    Article Title: Prkci acts a pro-proliferation factor in colorectal cancer.
    Article Snippet: .. Antibodies for Prkci (66493, Proteintech), Gapdh (ab181602, Abcam), Cdk4 (ab108357, Abcam), Glut1 (66290, Proteintech), HA-tag (66006, Proteintech), His-tag (66005, Proteintech), Flag-tag (66008, Proteintech), and pan Phospho-Serine/Threonine (AP1475, Abclonal) were purchased Fig. 7 | Prkci knockout inhibits tumor growth and improves survival in a colorectal cancermousemodel. ..



    Similar Products

    buffer  (New England Biolabs)
    99
    New England Biolabs buffer
    Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+protein+phosphatase+buffer/Lambda+Protein+Phosphatase/pm40957900-256-15-17
    Average 99 stars, based on 1 article reviews
    buffer - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    phosphatase buffer  (New England Biolabs)
    99
    New England Biolabs phosphatase buffer
    (A) Western blot analysis of cleared bacterial lysates of GST-PFKFB2 and mutants. Top: Total protein staining of cleared bacterial lysates over-expressing GST-PFKFB2 and mutants. Bottom: Western blots against GST and phosphorylated-PFKFB2 (Ser483) for GST-PFKFB2 and mutants. “Double mut” indicates protein containing both K174G and H259A. (B) Western blot analysis of GST-PFKFB2 expression, purification, and in vitro phosphorylation. Flow thru indicates proteins unbound to GSH beads. Column are proteins bound to the GSH beads. Column + Thrombin are proteins remaining bound to GSH beads after thrombin cleavage. Purified PFK2 lanes are proteins eluted from the GSH beads with thrombin cleavage. Purified PFK2 + ATP are proteins incubated with ATP alone. Purified PFK2 + ATP + PKA are proteins phosphorylated by PKA. (C) Western blot analysis of GST-PFKFB2 and mutant proteins showing they are phosphorylated (Ser483) upon extraction from bacteria ( left lanes ), lose phosphorylation after purification ( middle lanes ), but are re-phosphorylated upon treatment with PKA and ATP ( right lanes ). (D) Representative western blot against phosphorylated-PFKFB2 (Ser483) of bacterial samples (WT PFKFB2) treated/not treated with <t>λ-phosphatase</t> for indicated times before lysis. Equal amounts of purified PFKFB2 were treated and loaded into each lane. Quantitation of bacterial samples treated with λ-phosphatase. Data are shown as mean±SD. The original, unedited blots are included in .
    Phosphatase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+protein+phosphatase+buffer/Lambda+Protein+Phosphatase/pmc12097583-49-16-21
    Average 99 stars, based on 1 article reviews
    phosphatase buffer - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    lambda phosphatase assay buffer  (New England Biolabs)
    99
    New England Biolabs lambda phosphatase assay buffer
    (A) Western blot analysis of cleared bacterial lysates of GST-PFKFB2 and mutants. Top: Total protein staining of cleared bacterial lysates over-expressing GST-PFKFB2 and mutants. Bottom: Western blots against GST and phosphorylated-PFKFB2 (Ser483) for GST-PFKFB2 and mutants. “Double mut” indicates protein containing both K174G and H259A. (B) Western blot analysis of GST-PFKFB2 expression, purification, and in vitro phosphorylation. Flow thru indicates proteins unbound to GSH beads. Column are proteins bound to the GSH beads. Column + Thrombin are proteins remaining bound to GSH beads after thrombin cleavage. Purified PFK2 lanes are proteins eluted from the GSH beads with thrombin cleavage. Purified PFK2 + ATP are proteins incubated with ATP alone. Purified PFK2 + ATP + PKA are proteins phosphorylated by PKA. (C) Western blot analysis of GST-PFKFB2 and mutant proteins showing they are phosphorylated (Ser483) upon extraction from bacteria ( left lanes ), lose phosphorylation after purification ( middle lanes ), but are re-phosphorylated upon treatment with PKA and ATP ( right lanes ). (D) Representative western blot against phosphorylated-PFKFB2 (Ser483) of bacterial samples (WT PFKFB2) treated/not treated with <t>λ-phosphatase</t> for indicated times before lysis. Equal amounts of purified PFKFB2 were treated and loaded into each lane. Quantitation of bacterial samples treated with λ-phosphatase. Data are shown as mean±SD. The original, unedited blots are included in .
    Lambda Phosphatase Assay Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+protein+phosphatase+buffer/Lambda+Protein+Phosphatase/pmc12049159-534-21-32
    Average 99 stars, based on 1 article reviews
    lambda phosphatase assay buffer - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Western blot analysis of cleared bacterial lysates of GST-PFKFB2 and mutants. Top: Total protein staining of cleared bacterial lysates over-expressing GST-PFKFB2 and mutants. Bottom: Western blots against GST and phosphorylated-PFKFB2 (Ser483) for GST-PFKFB2 and mutants. “Double mut” indicates protein containing both K174G and H259A. (B) Western blot analysis of GST-PFKFB2 expression, purification, and in vitro phosphorylation. Flow thru indicates proteins unbound to GSH beads. Column are proteins bound to the GSH beads. Column + Thrombin are proteins remaining bound to GSH beads after thrombin cleavage. Purified PFK2 lanes are proteins eluted from the GSH beads with thrombin cleavage. Purified PFK2 + ATP are proteins incubated with ATP alone. Purified PFK2 + ATP + PKA are proteins phosphorylated by PKA. (C) Western blot analysis of GST-PFKFB2 and mutant proteins showing they are phosphorylated (Ser483) upon extraction from bacteria ( left lanes ), lose phosphorylation after purification ( middle lanes ), but are re-phosphorylated upon treatment with PKA and ATP ( right lanes ). (D) Representative western blot against phosphorylated-PFKFB2 (Ser483) of bacterial samples (WT PFKFB2) treated/not treated with λ-phosphatase for indicated times before lysis. Equal amounts of purified PFKFB2 were treated and loaded into each lane. Quantitation of bacterial samples treated with λ-phosphatase. Data are shown as mean±SD. The original, unedited blots are included in .

    Journal: PLOS One

    Article Title: Mechanistic studies of PFKFB2 reveal a novel inhibitor of its kinase activity

    doi: 10.1371/journal.pone.0317167

    Figure Lengend Snippet: (A) Western blot analysis of cleared bacterial lysates of GST-PFKFB2 and mutants. Top: Total protein staining of cleared bacterial lysates over-expressing GST-PFKFB2 and mutants. Bottom: Western blots against GST and phosphorylated-PFKFB2 (Ser483) for GST-PFKFB2 and mutants. “Double mut” indicates protein containing both K174G and H259A. (B) Western blot analysis of GST-PFKFB2 expression, purification, and in vitro phosphorylation. Flow thru indicates proteins unbound to GSH beads. Column are proteins bound to the GSH beads. Column + Thrombin are proteins remaining bound to GSH beads after thrombin cleavage. Purified PFK2 lanes are proteins eluted from the GSH beads with thrombin cleavage. Purified PFK2 + ATP are proteins incubated with ATP alone. Purified PFK2 + ATP + PKA are proteins phosphorylated by PKA. (C) Western blot analysis of GST-PFKFB2 and mutant proteins showing they are phosphorylated (Ser483) upon extraction from bacteria ( left lanes ), lose phosphorylation after purification ( middle lanes ), but are re-phosphorylated upon treatment with PKA and ATP ( right lanes ). (D) Representative western blot against phosphorylated-PFKFB2 (Ser483) of bacterial samples (WT PFKFB2) treated/not treated with λ-phosphatase for indicated times before lysis. Equal amounts of purified PFKFB2 were treated and loaded into each lane. Quantitation of bacterial samples treated with λ-phosphatase. Data are shown as mean±SD. The original, unedited blots are included in .

    Article Snippet: Assay conditions were 100 µL sonicated sample, 10 µL MnCl 2 (10 mM NEB), 10 µL phosphatase buffer (10x PMP buffer, NEB) with or without 2 µL Lambda protein phosphatase (NEB, 400000 U/mL).

    Techniques: Western Blot, Staining, Expressing, Purification, In Vitro, Phospho-proteomics, Incubation, Mutagenesis, Extraction, Bacteria, Lysis, Quantitation Assay