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serum m protein levels  (Bethyl)


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    Bethyl serum m protein levels
    Serum M Protein Levels, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+protein+phosphatase+buffer/Human+Lambda+Free+Light+Chain/pm36385211-71-0-11
    Average 93 stars, based on 36 article reviews
    serum m protein levels - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: Anti-lambda myeloma antigen (LMA) binding proteins to treat LMA-expressing cancer and autoimmune disorders
    Article Snippet: .. Similarly, human anti-LMA antibodies (6A1, 13H3 and 4A1) demonstrated high and selective affinity to human multiple myeloma cell lines expressing lambda-light chain isotypes and not to kappa-light chain expressing JJN3 cell line (FIG. 4) while clones 18E8, 7F11 and 18F9 demonstrated selective affinity to commercial lambda-light chain (Bethyl) (FIG. 2B). ..

    Clone Assay:

    Article Title: Anti-lambda myeloma antigen (LMA) binding proteins to treat LMA-expressing cancer and autoimmune disorders
    Article Snippet: .. Similarly, human anti-LMA antibodies (6A1, 13H3 and 4A1) demonstrated high and selective affinity to human multiple myeloma cell lines expressing lambda-light chain isotypes and not to kappa-light chain expressing JJN3 cell line (FIG. 4) while clones 18E8, 7F11 and 18F9 demonstrated selective affinity to commercial lambda-light chain (Bethyl) (FIG. 2B). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Heterohybridomas producing human immunoglobulin light chains using CD138-selected bone marrow cells.
    Article Snippet: Background: Light chain research is hampered by lack of mammalian cell lines producing human light chains (FLC).. Therefore, we used heterohybridoma (HH) technology to produce clones making FLC thereby providing tools to study light chain behavior.. Methods: Marrow CD138+ cells from patients with multiple myeloma (MM) and polyclonal gammopathy (PG) were selected, fused with B5-6 T cells and cultured in hypoxanthine-aminopterin-thymidine medium (HAT).

    Article Title: Treatment of al amyloidosis with melflufen
    Article Snippet: .. Levels of kappa and lambda light chains as well as BCMA from the serum was analysed on days 9 and 16 post tumor inoculation using commercial ELISA kits (below) and manufacturer's protocols: Human Kappa ELISA (Bethyl Laboratories, #E80-115) Human Lambda ELISA (Bethyl Laboratories, #E80-116) Human BCMA ELISA (R&D Systems, #DY193). ..

    Article Title: Heterohybridomas producing human immunoglobulin light chains using CD138-selected bone marrow cells
    Article Snippet: .. Heterohybridoma supernatants for ELISA were evaluated in quantitative sandwich ELISA for human IgA, IgG, IgM, κ and λ light chains (Bethyl Laboratories, Montgomery, TX) according to the manufacturers’ instructions. ..

    Article Title: Therapeutic implications of mitochondrial stress-induced proteasome inhibitor resistance in multiple myeloma.
    Article Snippet: .. Lysates of the cell pellets were prepared in RIPA buffer, and the lambda and kappa light chain antibodies were measured using Bethyl Human Lambda ELISA Quantitation Set (E80-116) and Bethyl Human Kappa ELISA Quantitation Set (#E80-115) following the manufacturer’s protocol. .. Bacterial clones containing ATF4 short hairpin RNAs were obtained from Sigma-Aldrich (TRCN0000013573 and TRCN0000013574) and plated on LB agar plates containing ampicillin.

    Article Title: Therapeutic implications of mitochondrial stress–induced proteasome inhibitor resistance in multiple myeloma
    Article Snippet: .. Lysates of the cell pellets were prepared in RIPA buffer, and the lambda and kappa light chain antibodies were measured using Bethyl Human Lambda ELISA Quantitation Set (E80-116) and Bethyl Human Kappa ELISA Quantitation Set (#E80-115) following the manufacturer’s protocol. .. Bacterial clones containing ATF4 short hairpin RNAs were obtained from Sigma-Aldrich (TRCN0000013573 and TRCN0000013574) and plated on LB agar plates containing ampicillin.

    Article Title: A non-ligand surrogate agonist antibody that enhances canonical Wnt signaling and bone regeneration
    Article Snippet: .. One week post treatment, mice were anesthetized and scanned by micro-CT. One week post CT scanning, blood and femurs were collected from the mice, and free human Ig-lambda light chains in serum was assessed using a Human Lambda ELISA Kit (Bethyl Laboratories) according to manufacturer’s instructions. ..

    Sandwich ELISA:

    Article Title: Heterohybridomas producing human immunoglobulin light chains using CD138-selected bone marrow cells.
    Article Snippet: Background: Light chain research is hampered by lack of mammalian cell lines producing human light chains (FLC).. Therefore, we used heterohybridoma (HH) technology to produce clones making FLC thereby providing tools to study light chain behavior.. Methods: Marrow CD138+ cells from patients with multiple myeloma (MM) and polyclonal gammopathy (PG) were selected, fused with B5-6 T cells and cultured in hypoxanthine-aminopterin-thymidine medium (HAT).

    Article Title: Heterohybridomas producing human immunoglobulin light chains using CD138-selected bone marrow cells
    Article Snippet: .. Heterohybridoma supernatants for ELISA were evaluated in quantitative sandwich ELISA for human IgA, IgG, IgM, κ and λ light chains (Bethyl Laboratories, Montgomery, TX) according to the manufacturers’ instructions. ..

    Quantitation Assay:

    Article Title: Therapeutic implications of mitochondrial stress-induced proteasome inhibitor resistance in multiple myeloma.
    Article Snippet: .. Lysates of the cell pellets were prepared in RIPA buffer, and the lambda and kappa light chain antibodies were measured using Bethyl Human Lambda ELISA Quantitation Set (E80-116) and Bethyl Human Kappa ELISA Quantitation Set (#E80-115) following the manufacturer’s protocol. .. Bacterial clones containing ATF4 short hairpin RNAs were obtained from Sigma-Aldrich (TRCN0000013573 and TRCN0000013574) and plated on LB agar plates containing ampicillin.

    Article Title: Therapeutic implications of mitochondrial stress–induced proteasome inhibitor resistance in multiple myeloma
    Article Snippet: .. Lysates of the cell pellets were prepared in RIPA buffer, and the lambda and kappa light chain antibodies were measured using Bethyl Human Lambda ELISA Quantitation Set (E80-116) and Bethyl Human Kappa ELISA Quantitation Set (#E80-115) following the manufacturer’s protocol. .. Bacterial clones containing ATF4 short hairpin RNAs were obtained from Sigma-Aldrich (TRCN0000013573 and TRCN0000013574) and plated on LB agar plates containing ampicillin.

    Micro-CT:

    Article Title: A non-ligand surrogate agonist antibody that enhances canonical Wnt signaling and bone regeneration
    Article Snippet: .. One week post treatment, mice were anesthetized and scanned by micro-CT. One week post CT scanning, blood and femurs were collected from the mice, and free human Ig-lambda light chains in serum was assessed using a Human Lambda ELISA Kit (Bethyl Laboratories) according to manufacturer’s instructions. ..



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    (A) Western blot analysis of cleared bacterial lysates of GST-PFKFB2 and mutants. Top: Total protein staining of cleared bacterial lysates over-expressing GST-PFKFB2 and mutants. Bottom: Western blots against GST and phosphorylated-PFKFB2 (Ser483) for GST-PFKFB2 and mutants. “Double mut” indicates protein containing both K174G and H259A. (B) Western blot analysis of GST-PFKFB2 expression, purification, and in vitro phosphorylation. Flow thru indicates proteins unbound to GSH beads. Column are proteins bound to the GSH beads. Column + Thrombin are proteins remaining bound to GSH beads after thrombin cleavage. Purified PFK2 lanes are proteins eluted from the GSH beads with thrombin cleavage. Purified PFK2 + ATP are proteins incubated with ATP alone. Purified PFK2 + ATP + PKA are proteins phosphorylated by PKA. (C) Western blot analysis of GST-PFKFB2 and mutant proteins showing they are phosphorylated (Ser483) upon extraction from bacteria ( left lanes ), lose phosphorylation after purification ( middle lanes ), but are re-phosphorylated upon treatment with PKA and ATP ( right lanes ). (D) Representative western blot against phosphorylated-PFKFB2 (Ser483) of bacterial samples (WT PFKFB2) treated/not treated with <t>λ-phosphatase</t> for indicated times before lysis. Equal amounts of purified PFKFB2 were treated and loaded into each lane. Quantitation of bacterial samples treated with λ-phosphatase. Data are shown as mean±SD. The original, unedited blots are included in .
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    (A) Western blot analysis of cleared bacterial lysates of GST-PFKFB2 and mutants. Top: Total protein staining of cleared bacterial lysates over-expressing GST-PFKFB2 and mutants. Bottom: Western blots against GST and phosphorylated-PFKFB2 (Ser483) for GST-PFKFB2 and mutants. “Double mut” indicates protein containing both K174G and H259A. (B) Western blot analysis of GST-PFKFB2 expression, purification, and in vitro phosphorylation. Flow thru indicates proteins unbound to GSH beads. Column are proteins bound to the GSH beads. Column + Thrombin are proteins remaining bound to GSH beads after thrombin cleavage. Purified PFK2 lanes are proteins eluted from the GSH beads with thrombin cleavage. Purified PFK2 + ATP are proteins incubated with ATP alone. Purified PFK2 + ATP + PKA are proteins phosphorylated by PKA. (C) Western blot analysis of GST-PFKFB2 and mutant proteins showing they are phosphorylated (Ser483) upon extraction from bacteria ( left lanes ), lose phosphorylation after purification ( middle lanes ), but are re-phosphorylated upon treatment with PKA and ATP ( right lanes ). (D) Representative western blot against phosphorylated-PFKFB2 (Ser483) of bacterial samples (WT PFKFB2) treated/not treated with <t>λ-phosphatase</t> for indicated times before lysis. Equal amounts of purified PFKFB2 were treated and loaded into each lane. Quantitation of bacterial samples treated with λ-phosphatase. Data are shown as mean±SD. The original, unedited blots are included in .
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    Image Search Results


    (A) Western blot analysis of cleared bacterial lysates of GST-PFKFB2 and mutants. Top: Total protein staining of cleared bacterial lysates over-expressing GST-PFKFB2 and mutants. Bottom: Western blots against GST and phosphorylated-PFKFB2 (Ser483) for GST-PFKFB2 and mutants. “Double mut” indicates protein containing both K174G and H259A. (B) Western blot analysis of GST-PFKFB2 expression, purification, and in vitro phosphorylation. Flow thru indicates proteins unbound to GSH beads. Column are proteins bound to the GSH beads. Column + Thrombin are proteins remaining bound to GSH beads after thrombin cleavage. Purified PFK2 lanes are proteins eluted from the GSH beads with thrombin cleavage. Purified PFK2 + ATP are proteins incubated with ATP alone. Purified PFK2 + ATP + PKA are proteins phosphorylated by PKA. (C) Western blot analysis of GST-PFKFB2 and mutant proteins showing they are phosphorylated (Ser483) upon extraction from bacteria ( left lanes ), lose phosphorylation after purification ( middle lanes ), but are re-phosphorylated upon treatment with PKA and ATP ( right lanes ). (D) Representative western blot against phosphorylated-PFKFB2 (Ser483) of bacterial samples (WT PFKFB2) treated/not treated with λ-phosphatase for indicated times before lysis. Equal amounts of purified PFKFB2 were treated and loaded into each lane. Quantitation of bacterial samples treated with λ-phosphatase. Data are shown as mean±SD. The original, unedited blots are included in .

    Journal: PLOS One

    Article Title: Mechanistic studies of PFKFB2 reveal a novel inhibitor of its kinase activity

    doi: 10.1371/journal.pone.0317167

    Figure Lengend Snippet: (A) Western blot analysis of cleared bacterial lysates of GST-PFKFB2 and mutants. Top: Total protein staining of cleared bacterial lysates over-expressing GST-PFKFB2 and mutants. Bottom: Western blots against GST and phosphorylated-PFKFB2 (Ser483) for GST-PFKFB2 and mutants. “Double mut” indicates protein containing both K174G and H259A. (B) Western blot analysis of GST-PFKFB2 expression, purification, and in vitro phosphorylation. Flow thru indicates proteins unbound to GSH beads. Column are proteins bound to the GSH beads. Column + Thrombin are proteins remaining bound to GSH beads after thrombin cleavage. Purified PFK2 lanes are proteins eluted from the GSH beads with thrombin cleavage. Purified PFK2 + ATP are proteins incubated with ATP alone. Purified PFK2 + ATP + PKA are proteins phosphorylated by PKA. (C) Western blot analysis of GST-PFKFB2 and mutant proteins showing they are phosphorylated (Ser483) upon extraction from bacteria ( left lanes ), lose phosphorylation after purification ( middle lanes ), but are re-phosphorylated upon treatment with PKA and ATP ( right lanes ). (D) Representative western blot against phosphorylated-PFKFB2 (Ser483) of bacterial samples (WT PFKFB2) treated/not treated with λ-phosphatase for indicated times before lysis. Equal amounts of purified PFKFB2 were treated and loaded into each lane. Quantitation of bacterial samples treated with λ-phosphatase. Data are shown as mean±SD. The original, unedited blots are included in .

    Article Snippet: Assay conditions were 100 µL sonicated sample, 10 µL MnCl 2 (10 mM NEB), 10 µL phosphatase buffer (10x PMP buffer, NEB) with or without 2 µL Lambda protein phosphatase (NEB, 400000 U/mL).

    Techniques: Western Blot, Staining, Expressing, Purification, In Vitro, Phospho-proteomics, Incubation, Mutagenesis, Extraction, Bacteria, Lysis, Quantitation Assay