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ssc buffer 20x thermofisher scientific  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ssc buffer 20x thermofisher scientific
    Ssc Buffer 20x Thermofisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+protein+phosphatase+buffer/SSC+buffer/pm36099913-254-240-243
    Average 97 stars, based on 1 article reviews
    ssc buffer 20x thermofisher scientific - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: RNA-binding proteins in the mouse lens: Functional classifications, expression profiling, and interaction studies of Carhsp1 with crystallin mRNAs.
    Article Snippet: RNA-binding proteins (RBPs) are critical regulators of mRNAs controlling all processes such as RNA transcription, transport, localization, translation, mRNA:ncRNA interactions, and decay.. Cellular differentiation is driven by temporally and spatially regulated expression of proteins needed for the optimal function of individual cells, tissues and organs.. Lens fiber cell differentiation is marked by high levels of expression of crystallin genes encoding critical proteins for lens transparency and light refraction.

    Article Title: Single-cell Stereo-seq reveals regulatory mechanisms driving regeneration of injured proximal tubules during AKI.
    Article Snippet: .. After washed with 0.1× SSC buffer (Thermo, AM9770) supplemented with 0.05 U/mL RNase inhibitor (NEB, M0314L), tissue sections placed on the chip were permeabilized using 0.1% pepsin (Sigma, P7000) in 0.01 M HCl buffer, incubated at 37°C for 5 minutes and then washed with 0.1×SSC buffer (Thermo, AM9770) supplemented with 0.05 U/mL RNase inhibitor (NEB, M0314L). .. RNA released from the permeabilized tissue and captured by the DNB was reverse transcribed 2h at 42°C using SuperScript II (Invitrogen, 18064-014, 10 U/mL reverse transcriptase, 1 mM dNTPs, 1 M betaine solution PCR reagent, 7.5 mM MgCl2, 5 mM DTT, 2 U/mL RNase inhibitor, 2.5 mM Stereo-seq-TSO and 1×First-Strand buffer).

    Concentration Assay:

    Article Title: RNA-binding proteins in the mouse lens: Functional classifications, expression profiling, and interaction studies of Carhsp1 with crystallin mRNAs.
    Article Snippet: RNA-binding proteins (RBPs) are critical regulators of mRNAs controlling all processes such as RNA transcription, transport, localization, translation, mRNA:ncRNA interactions, and decay.. Cellular differentiation is driven by temporally and spatially regulated expression of proteins needed for the optimal function of individual cells, tissues and organs.. Lens fiber cell differentiation is marked by high levels of expression of crystallin genes encoding critical proteins for lens transparency and light refraction.

    Hybridization:

    Article Title: RNA-binding proteins in the mouse lens: Functional classifications, expression profiling, and interaction studies of Carhsp1 with crystallin mRNAs.
    Article Snippet: RNA-binding proteins (RBPs) are critical regulators of mRNAs controlling all processes such as RNA transcription, transport, localization, translation, mRNA:ncRNA interactions, and decay.. Cellular differentiation is driven by temporally and spatially regulated expression of proteins needed for the optimal function of individual cells, tissues and organs.. Lens fiber cell differentiation is marked by high levels of expression of crystallin genes encoding critical proteins for lens transparency and light refraction.

    Saline:

    Article Title: DDERMMAL , a melanocytic long non-coding RNA, confers DNA damage tolerance to melanoma cells
    Article Snippet: Formamide , Sigma-Aldrich , Cat# F9037. .. Saline sodium citrate (20X) , Thermo Fisher Scientific , Cat# J60839.K2. .. ProLongTM Glass Antifade Mountant , Thermo Fisher Scientific , Cat# P36982.

    Article Title: The role of cell growth rate on accumulation of the mitotic cyclin Cdc13 in fission yeast
    Article Snippet: For each sample, approximately 20–25 ng of Stellaris CAL Fluor 610 probes targeting the mEGFP coding sequence (Table S2) was mixed with 2 μL of yeast tRNA (Thermo Fisher Scientific, AM7119), 2 μL of Herring Sperm DNA (Sigma, D7290), and 45 μL of Buffer F (20% formamide, 10 mM sodium-phosphate buffer pH 7.2). .. This mixture was heated at 95°C for 3 minutes and allowed to cool to room temperature before combining with 50 μL of Buffer H (4x saline-sodium citrate (SSC) buffer, 4 mg/ml acetylated BSA (Thermo Fisher Scientific, 2614G1), 20 mM vanadyl ribonuclease complex) to create the final probe solution. ..

    Article Title: Live-cell imaging of endogenous Egr1 mRNA using an MPBS knock-in mouse model
    Article Snippet: Formamide , Sigma-Aldrich , Cat# F9037-100 mL. .. Saline sodium citrate (SSC) buffer , Invitrogen , Cat# AM9763. .. Bovine serum albumin (BSA) , Sigma , Cat# A7906.

    Polymerase Chain Reaction:

    Article Title: The genetic legacy of the 17th-century colonial capital of St. Mary's City.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples 2 Calvert Individuals (Phillip & Son) Reich et al.8 N/A 47 newly reported St. Mary’s individuals This paper N/A Chemicals, peptides, and recombinant proteins 23 HI-RPM hybridization buffer Agilent Technologies 5190-0403 Herculase II fusion DNA polymerase Agilent Technologies 600679 Pfu turbo Cx Hotstart DNA polymerase Agilent Technologies 600412 50% PEG 8000 Anatrace OPTIMIZE-82 100 ML 0.5 M EDTA pH 8.0 BioExpress E177 Sera-Mag SpeedBead CarboxylateModified [E3] magnetic particles Cytiva Life Sciences 65152105050250 Silica magnetic beads G-Biosciences 786–916 10 3 T4 RNA ligase buffer New England Biolabs B0216L Bst DNA Polymerase2.0, large frag. .. New England Biolabs M0537 UGI New England Biolabs M0281 USER enzyme New England Biolabs M5505 Buffer PB QIAGEN 19066 Buffer PE concentrate QIAGEN 19065 1 M Tris-HCl pH 8.0 Sigma Aldrich AM9856 1 M NaOH Sigma Aldrich 71463 20% SDS Sigma Aldrich 5030 3 M Sodium Acetate (pH 5.2) Sigma Aldrich S7899 5 M NaCl Sigma Aldrich S5150 Ethanol Sigma Aldrich E7023 Guanidine hydrochloride Sigma Aldrich G3272 Isopropanol Sigma Aldrich 650447 PEG-8000 Sigma Aldrich 89510 Proteinase K Sigma Aldrich P6556 Tween-20 Sigma Aldrich P9416 Water Sigma Aldrich W4502 103 Buffer Tango Thermo Fisher Scientific BY5 503 Denhardt’s solution Thermo Fisher Scientific 750018 AccuPrime Pfx Polymerase (2.5 U/ul) Thermo Fisher Scientific 12344032 ATP Thermo Fisher Scientific R0441 dNTP Mix Thermo Fisher Scientific R1121 Dyna MyOne Streptavidin C1 beads Thermo Fisher Scientific 65002 FastAP (1 U/mL) Thermo Fisher Scientific EF0651 GeneAmp 103 PCR Gold Buffer Thermo Fisher Scientific 4379874 Human Cot-I DNA Thermo Fisher Scientific 15279011 Klenow Fragment (10 U/mL) Thermo Fisher Scientific EP0052 Maxima Probe qPCR 2xMM Thermo Fisher Scientific K0233 Maxima SYBR Green kit Thermo Fisher Scientific K0251 Maxima SYBR Green kit Thermo Fisher Scientific K0253 Salmon sperm DNA Thermo Fisher Scientific 15632-011 SSC Buffer (203) Thermo Fisher Scientific AM9770 (Continued on next page) ll OPEN ACCESS e1 Current Biology 36, 1–13.e1–e6, June 8, 2026 Article .. This study was made possible by the millions of research participants who actively consented to participate in genetic research (including those from 23andMe and others whose data has been released as part of publicly available datasets).

    Real-time Polymerase Chain Reaction:

    Article Title: The genetic legacy of the 17th-century colonial capital of St. Mary's City.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples 2 Calvert Individuals (Phillip & Son) Reich et al.8 N/A 47 newly reported St. Mary’s individuals This paper N/A Chemicals, peptides, and recombinant proteins 23 HI-RPM hybridization buffer Agilent Technologies 5190-0403 Herculase II fusion DNA polymerase Agilent Technologies 600679 Pfu turbo Cx Hotstart DNA polymerase Agilent Technologies 600412 50% PEG 8000 Anatrace OPTIMIZE-82 100 ML 0.5 M EDTA pH 8.0 BioExpress E177 Sera-Mag SpeedBead CarboxylateModified [E3] magnetic particles Cytiva Life Sciences 65152105050250 Silica magnetic beads G-Biosciences 786–916 10 3 T4 RNA ligase buffer New England Biolabs B0216L Bst DNA Polymerase2.0, large frag. .. New England Biolabs M0537 UGI New England Biolabs M0281 USER enzyme New England Biolabs M5505 Buffer PB QIAGEN 19066 Buffer PE concentrate QIAGEN 19065 1 M Tris-HCl pH 8.0 Sigma Aldrich AM9856 1 M NaOH Sigma Aldrich 71463 20% SDS Sigma Aldrich 5030 3 M Sodium Acetate (pH 5.2) Sigma Aldrich S7899 5 M NaCl Sigma Aldrich S5150 Ethanol Sigma Aldrich E7023 Guanidine hydrochloride Sigma Aldrich G3272 Isopropanol Sigma Aldrich 650447 PEG-8000 Sigma Aldrich 89510 Proteinase K Sigma Aldrich P6556 Tween-20 Sigma Aldrich P9416 Water Sigma Aldrich W4502 103 Buffer Tango Thermo Fisher Scientific BY5 503 Denhardt’s solution Thermo Fisher Scientific 750018 AccuPrime Pfx Polymerase (2.5 U/ul) Thermo Fisher Scientific 12344032 ATP Thermo Fisher Scientific R0441 dNTP Mix Thermo Fisher Scientific R1121 Dyna MyOne Streptavidin C1 beads Thermo Fisher Scientific 65002 FastAP (1 U/mL) Thermo Fisher Scientific EF0651 GeneAmp 103 PCR Gold Buffer Thermo Fisher Scientific 4379874 Human Cot-I DNA Thermo Fisher Scientific 15279011 Klenow Fragment (10 U/mL) Thermo Fisher Scientific EP0052 Maxima Probe qPCR 2xMM Thermo Fisher Scientific K0233 Maxima SYBR Green kit Thermo Fisher Scientific K0251 Maxima SYBR Green kit Thermo Fisher Scientific K0253 Salmon sperm DNA Thermo Fisher Scientific 15632-011 SSC Buffer (203) Thermo Fisher Scientific AM9770 (Continued on next page) ll OPEN ACCESS e1 Current Biology 36, 1–13.e1–e6, June 8, 2026 Article .. This study was made possible by the millions of research participants who actively consented to participate in genetic research (including those from 23andMe and others whose data has been released as part of publicly available datasets).

    SYBR Green Assay:

    Article Title: The genetic legacy of the 17th-century colonial capital of St. Mary's City.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples 2 Calvert Individuals (Phillip & Son) Reich et al.8 N/A 47 newly reported St. Mary’s individuals This paper N/A Chemicals, peptides, and recombinant proteins 23 HI-RPM hybridization buffer Agilent Technologies 5190-0403 Herculase II fusion DNA polymerase Agilent Technologies 600679 Pfu turbo Cx Hotstart DNA polymerase Agilent Technologies 600412 50% PEG 8000 Anatrace OPTIMIZE-82 100 ML 0.5 M EDTA pH 8.0 BioExpress E177 Sera-Mag SpeedBead CarboxylateModified [E3] magnetic particles Cytiva Life Sciences 65152105050250 Silica magnetic beads G-Biosciences 786–916 10 3 T4 RNA ligase buffer New England Biolabs B0216L Bst DNA Polymerase2.0, large frag. .. New England Biolabs M0537 UGI New England Biolabs M0281 USER enzyme New England Biolabs M5505 Buffer PB QIAGEN 19066 Buffer PE concentrate QIAGEN 19065 1 M Tris-HCl pH 8.0 Sigma Aldrich AM9856 1 M NaOH Sigma Aldrich 71463 20% SDS Sigma Aldrich 5030 3 M Sodium Acetate (pH 5.2) Sigma Aldrich S7899 5 M NaCl Sigma Aldrich S5150 Ethanol Sigma Aldrich E7023 Guanidine hydrochloride Sigma Aldrich G3272 Isopropanol Sigma Aldrich 650447 PEG-8000 Sigma Aldrich 89510 Proteinase K Sigma Aldrich P6556 Tween-20 Sigma Aldrich P9416 Water Sigma Aldrich W4502 103 Buffer Tango Thermo Fisher Scientific BY5 503 Denhardt’s solution Thermo Fisher Scientific 750018 AccuPrime Pfx Polymerase (2.5 U/ul) Thermo Fisher Scientific 12344032 ATP Thermo Fisher Scientific R0441 dNTP Mix Thermo Fisher Scientific R1121 Dyna MyOne Streptavidin C1 beads Thermo Fisher Scientific 65002 FastAP (1 U/mL) Thermo Fisher Scientific EF0651 GeneAmp 103 PCR Gold Buffer Thermo Fisher Scientific 4379874 Human Cot-I DNA Thermo Fisher Scientific 15279011 Klenow Fragment (10 U/mL) Thermo Fisher Scientific EP0052 Maxima Probe qPCR 2xMM Thermo Fisher Scientific K0233 Maxima SYBR Green kit Thermo Fisher Scientific K0251 Maxima SYBR Green kit Thermo Fisher Scientific K0253 Salmon sperm DNA Thermo Fisher Scientific 15632-011 SSC Buffer (203) Thermo Fisher Scientific AM9770 (Continued on next page) ll OPEN ACCESS e1 Current Biology 36, 1–13.e1–e6, June 8, 2026 Article .. This study was made possible by the millions of research participants who actively consented to participate in genetic research (including those from 23andMe and others whose data has been released as part of publicly available datasets).



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    (A) Western blot analysis of cleared bacterial lysates of GST-PFKFB2 and mutants. Top: Total protein staining of cleared bacterial lysates over-expressing GST-PFKFB2 and mutants. Bottom: Western blots against GST and phosphorylated-PFKFB2 (Ser483) for GST-PFKFB2 and mutants. “Double mut” indicates protein containing both K174G and H259A. (B) Western blot analysis of GST-PFKFB2 expression, purification, and in vitro phosphorylation. Flow thru indicates proteins unbound to GSH beads. Column are proteins bound to the GSH beads. Column + Thrombin are proteins remaining bound to GSH beads after thrombin cleavage. Purified PFK2 lanes are proteins eluted from the GSH beads with thrombin cleavage. Purified PFK2 + ATP are proteins incubated with ATP alone. Purified PFK2 + ATP + PKA are proteins phosphorylated by PKA. (C) Western blot analysis of GST-PFKFB2 and mutant proteins showing they are phosphorylated (Ser483) upon extraction from bacteria ( left lanes ), lose phosphorylation after purification ( middle lanes ), but are re-phosphorylated upon treatment with PKA and ATP ( right lanes ). (D) Representative western blot against phosphorylated-PFKFB2 (Ser483) of bacterial samples (WT PFKFB2) treated/not treated with <t>λ-phosphatase</t> for indicated times before lysis. Equal amounts of purified PFKFB2 were treated and loaded into each lane. Quantitation of bacterial samples treated with λ-phosphatase. Data are shown as mean±SD. The original, unedited blots are included in .
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    (A) Western blot analysis of cleared bacterial lysates of GST-PFKFB2 and mutants. Top: Total protein staining of cleared bacterial lysates over-expressing GST-PFKFB2 and mutants. Bottom: Western blots against GST and phosphorylated-PFKFB2 (Ser483) for GST-PFKFB2 and mutants. “Double mut” indicates protein containing both K174G and H259A. (B) Western blot analysis of GST-PFKFB2 expression, purification, and in vitro phosphorylation. Flow thru indicates proteins unbound to GSH beads. Column are proteins bound to the GSH beads. Column + Thrombin are proteins remaining bound to GSH beads after thrombin cleavage. Purified PFK2 lanes are proteins eluted from the GSH beads with thrombin cleavage. Purified PFK2 + ATP are proteins incubated with ATP alone. Purified PFK2 + ATP + PKA are proteins phosphorylated by PKA. (C) Western blot analysis of GST-PFKFB2 and mutant proteins showing they are phosphorylated (Ser483) upon extraction from bacteria ( left lanes ), lose phosphorylation after purification ( middle lanes ), but are re-phosphorylated upon treatment with PKA and ATP ( right lanes ). (D) Representative western blot against phosphorylated-PFKFB2 (Ser483) of bacterial samples (WT PFKFB2) treated/not treated with <t>λ-phosphatase</t> for indicated times before lysis. Equal amounts of purified PFKFB2 were treated and loaded into each lane. Quantitation of bacterial samples treated with λ-phosphatase. Data are shown as mean±SD. The original, unedited blots are included in .
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    Image Search Results


    (A) Western blot analysis of cleared bacterial lysates of GST-PFKFB2 and mutants. Top: Total protein staining of cleared bacterial lysates over-expressing GST-PFKFB2 and mutants. Bottom: Western blots against GST and phosphorylated-PFKFB2 (Ser483) for GST-PFKFB2 and mutants. “Double mut” indicates protein containing both K174G and H259A. (B) Western blot analysis of GST-PFKFB2 expression, purification, and in vitro phosphorylation. Flow thru indicates proteins unbound to GSH beads. Column are proteins bound to the GSH beads. Column + Thrombin are proteins remaining bound to GSH beads after thrombin cleavage. Purified PFK2 lanes are proteins eluted from the GSH beads with thrombin cleavage. Purified PFK2 + ATP are proteins incubated with ATP alone. Purified PFK2 + ATP + PKA are proteins phosphorylated by PKA. (C) Western blot analysis of GST-PFKFB2 and mutant proteins showing they are phosphorylated (Ser483) upon extraction from bacteria ( left lanes ), lose phosphorylation after purification ( middle lanes ), but are re-phosphorylated upon treatment with PKA and ATP ( right lanes ). (D) Representative western blot against phosphorylated-PFKFB2 (Ser483) of bacterial samples (WT PFKFB2) treated/not treated with λ-phosphatase for indicated times before lysis. Equal amounts of purified PFKFB2 were treated and loaded into each lane. Quantitation of bacterial samples treated with λ-phosphatase. Data are shown as mean±SD. The original, unedited blots are included in .

    Journal: PLOS One

    Article Title: Mechanistic studies of PFKFB2 reveal a novel inhibitor of its kinase activity

    doi: 10.1371/journal.pone.0317167

    Figure Lengend Snippet: (A) Western blot analysis of cleared bacterial lysates of GST-PFKFB2 and mutants. Top: Total protein staining of cleared bacterial lysates over-expressing GST-PFKFB2 and mutants. Bottom: Western blots against GST and phosphorylated-PFKFB2 (Ser483) for GST-PFKFB2 and mutants. “Double mut” indicates protein containing both K174G and H259A. (B) Western blot analysis of GST-PFKFB2 expression, purification, and in vitro phosphorylation. Flow thru indicates proteins unbound to GSH beads. Column are proteins bound to the GSH beads. Column + Thrombin are proteins remaining bound to GSH beads after thrombin cleavage. Purified PFK2 lanes are proteins eluted from the GSH beads with thrombin cleavage. Purified PFK2 + ATP are proteins incubated with ATP alone. Purified PFK2 + ATP + PKA are proteins phosphorylated by PKA. (C) Western blot analysis of GST-PFKFB2 and mutant proteins showing they are phosphorylated (Ser483) upon extraction from bacteria ( left lanes ), lose phosphorylation after purification ( middle lanes ), but are re-phosphorylated upon treatment with PKA and ATP ( right lanes ). (D) Representative western blot against phosphorylated-PFKFB2 (Ser483) of bacterial samples (WT PFKFB2) treated/not treated with λ-phosphatase for indicated times before lysis. Equal amounts of purified PFKFB2 were treated and loaded into each lane. Quantitation of bacterial samples treated with λ-phosphatase. Data are shown as mean±SD. The original, unedited blots are included in .

    Article Snippet: Assay conditions were 100 µL sonicated sample, 10 µL MnCl 2 (10 mM NEB), 10 µL phosphatase buffer (10x PMP buffer, NEB) with or without 2 µL Lambda protein phosphatase (NEB, 400000 U/mL).

    Techniques: Western Blot, Staining, Expressing, Purification, In Vitro, Phospho-proteomics, Incubation, Mutagenesis, Extraction, Bacteria, Lysis, Quantitation Assay