Concentration Assay:Article Title: Method for selecting polynucleotides based on enzyme interaction duration
Article Snippet: .. Briefly, the Lambda genomic DNA from NEB (N3013) was diluted to the concentration specified by the manufacturer in nuclease free water. ..
Lambda DNA Preparation:Article Title: A multiplex PCR-based sequencing method for the diagnosis and differentiation of echinococcosis using plasma samples, a proof-of-concept study.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human DNA (NA12878) CORIELL INSTITUTE CatNA12878 Lambda DNA NEB Cat#N3011S Echinococcus gDNA Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China N/A Human plasma samples The People’s Hospital of Ganzi Tibetan Autonomous Prefecture, Kangding, Sichuan Province, China N/A Critical commercial assays Diagnostic Kit for IgG Antibody to Hydatid (ELISA) Haitai Biotech Cat#20153400132 MagPure Circulating DNA KF Kit Magen Cat#MD5432-02 ATOPlex DNA Dual BC Library Prep Set MGI Cat#940-001191-00 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32854 Deposited data Sequencing data This paper CNSA: https://db.cngb.org/cnsa with accession number CNP0005472 Oligonucleotides Primer pairs and targeting genomic positions of the amplicons, see Tables S1 and S2. .. This study and primers from the literature (Wan, Z. et al.20 Spotin, A. et al.,23 Kinkar, L. et al.,24 Toribio, L. et al.25 N/A Software and algorithms ATOPlex Designer MGI https://atoplex.mgi-tech.com/ SOPAnuke v2.1.0 Chen, Y. et al.37 https://github.com/BGI-flexlab/SOAPnuke Fastp v0.23.2 Chen, S. et al.38 https://github.com/OpenGene/fastp Kraken v1.0 Wood, D.E. et al.39 https://ccb.jhu.edu/software/kraken/ Snap-aligner v1.0beta.23 N/A https://github.com/amplab/snap Prinseq v0.20.4 N/A (https://github.com/uwb-linux/prinseq BWA v0.7.19 Li, H. et al.40 https://github.com/lh3/bwa Samtools Danecek, P. et al.41 http://samtools.sourceforge.net/ Bedtools v2.31.0 Quinlan, A.R. et al.42 https://github.com/arq5x/bedtools2 R v4.3.0 R Project https://www.r-project.org/ Stata Stata Corp, College Station, TX, USA https://www.stata.com/ Biorender.com Biorender https://www.biorender.com/ Cell Reports Methods 6, 101428, June 15, 2026 e1 Article ll OPEN ACCESS Biological samples and clinical information collection Lanzhou Veterinary Research Institute provided Echinococcus gDNA.
Article Title: Compositions and methods for analyzing modified nucleotides
Article Snippet: .. TABLE 3 Methylation (NEBNext (APOBEC)) and bisulfite Illumina libraries (WGBS) have similar global methylation levels % Methylation (50 ng) % Methylation (10 ng) CpG CHG CHH CpG CHG CHH NA12878 NEBNext 53.7 ± 0.00 0.7 ± 0.07 0.7 ± 0.07 53.7 ± 0.07 0.8 ± 0.07 0.8 ± 0.14 WGBS 53.9 ± 0.42 0.9 ± 0.14 1.2 ± 0.14 52.6 ± 0.07 0.7 ± 0.00 0.9 ± 0.00 Lambda NEBNext 0.6 ± 0.00 0.6 ± 0.00 0.6 ± 0.00 0.7 ± 0.07 0.8 ± 0.07 0.8 ± 0.07 WgBs 0.9 ± 0.30 0.95 ± 0.20 1.1 ± 0.20 0.5 ± 0.00 0.5 ± 0.00 0.7 ± 0.00 The percentage methylation for NA12878 and unmethylated lambda DNA in CpG/CHG/CHH contexts using 50 ng and 10 ng DNA inputs are shown from NEBNext (APOBEC) and WGBS libraries. .. Reads were obtained from Illumina 2×100 base by NovaSeq sequencing.
Article Title: Serotypes, MIC-Based Antimicrobial Susceptibility, and Genotypic Diversity of Actinobacillus pleuropneumoniae Isolates from Diseased Pigs in Brazil
Article Snippet: Gels were stained for 40 min with SYBR Safe ® (Invitrogen, Carlsbad, CA, USA), and DNA fragments were visualized under UV illumination using a Gel Doc XR ® imaging system (Bio-Rad Laboratories, Hercules, CA, USA). .. Band sizes were estimated using a lambda DNA PFGE marker (New England BioLabs, Ipswich, MA, USA). .. Frequency distributions of serotypes and antimicrobial resistance profiles were analyzed using SPSS v16.0 (SPSS Inc., Chicago, IL, USA).
Clinical Proteomics:Article Title: A multiplex PCR-based sequencing method for the diagnosis and differentiation of echinococcosis using plasma samples, a proof-of-concept study.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human DNA (NA12878) CORIELL INSTITUTE CatNA12878 Lambda DNA NEB Cat#N3011S Echinococcus gDNA Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China N/A Human plasma samples The People’s Hospital of Ganzi Tibetan Autonomous Prefecture, Kangding, Sichuan Province, China N/A Critical commercial assays Diagnostic Kit for IgG Antibody to Hydatid (ELISA) Haitai Biotech Cat#20153400132 MagPure Circulating DNA KF Kit Magen Cat#MD5432-02 ATOPlex DNA Dual BC Library Prep Set MGI Cat#940-001191-00 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32854 Deposited data Sequencing data This paper CNSA: https://db.cngb.org/cnsa with accession number CNP0005472 Oligonucleotides Primer pairs and targeting genomic positions of the amplicons, see Tables S1 and S2. .. This study and primers from the literature (Wan, Z. et al.20 Spotin, A. et al.,23 Kinkar, L. et al.,24 Toribio, L. et al.25 N/A Software and algorithms ATOPlex Designer MGI https://atoplex.mgi-tech.com/ SOPAnuke v2.1.0 Chen, Y. et al.37 https://github.com/BGI-flexlab/SOAPnuke Fastp v0.23.2 Chen, S. et al.38 https://github.com/OpenGene/fastp Kraken v1.0 Wood, D.E. et al.39 https://ccb.jhu.edu/software/kraken/ Snap-aligner v1.0beta.23 N/A https://github.com/amplab/snap Prinseq v0.20.4 N/A (https://github.com/uwb-linux/prinseq BWA v0.7.19 Li, H. et al.40 https://github.com/lh3/bwa Samtools Danecek, P. et al.41 http://samtools.sourceforge.net/ Bedtools v2.31.0 Quinlan, A.R. et al.42 https://github.com/arq5x/bedtools2 R v4.3.0 R Project https://www.r-project.org/ Stata Stata Corp, College Station, TX, USA https://www.stata.com/ Biorender.com Biorender https://www.biorender.com/ Cell Reports Methods 6, 101428, June 15, 2026 e1 Article ll OPEN ACCESS Biological samples and clinical information collection Lanzhou Veterinary Research Institute provided Echinococcus gDNA.
Diagnostic Assay:Article Title: A multiplex PCR-based sequencing method for the diagnosis and differentiation of echinococcosis using plasma samples, a proof-of-concept study.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human DNA (NA12878) CORIELL INSTITUTE CatNA12878 Lambda DNA NEB Cat#N3011S Echinococcus gDNA Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China N/A Human plasma samples The People’s Hospital of Ganzi Tibetan Autonomous Prefecture, Kangding, Sichuan Province, China N/A Critical commercial assays Diagnostic Kit for IgG Antibody to Hydatid (ELISA) Haitai Biotech Cat#20153400132 MagPure Circulating DNA KF Kit Magen Cat#MD5432-02 ATOPlex DNA Dual BC Library Prep Set MGI Cat#940-001191-00 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32854 Deposited data Sequencing data This paper CNSA: https://db.cngb.org/cnsa with accession number CNP0005472 Oligonucleotides Primer pairs and targeting genomic positions of the amplicons, see Tables S1 and S2. .. This study and primers from the literature (Wan, Z. et al.20 Spotin, A. et al.,23 Kinkar, L. et al.,24 Toribio, L. et al.25 N/A Software and algorithms ATOPlex Designer MGI https://atoplex.mgi-tech.com/ SOPAnuke v2.1.0 Chen, Y. et al.37 https://github.com/BGI-flexlab/SOAPnuke Fastp v0.23.2 Chen, S. et al.38 https://github.com/OpenGene/fastp Kraken v1.0 Wood, D.E. et al.39 https://ccb.jhu.edu/software/kraken/ Snap-aligner v1.0beta.23 N/A https://github.com/amplab/snap Prinseq v0.20.4 N/A (https://github.com/uwb-linux/prinseq BWA v0.7.19 Li, H. et al.40 https://github.com/lh3/bwa Samtools Danecek, P. et al.41 http://samtools.sourceforge.net/ Bedtools v2.31.0 Quinlan, A.R. et al.42 https://github.com/arq5x/bedtools2 R v4.3.0 R Project https://www.r-project.org/ Stata Stata Corp, College Station, TX, USA https://www.stata.com/ Biorender.com Biorender https://www.biorender.com/ Cell Reports Methods 6, 101428, June 15, 2026 e1 Article ll OPEN ACCESS Biological samples and clinical information collection Lanzhou Veterinary Research Institute provided Echinococcus gDNA.
Enzyme-linked Immunosorbent Assay:Article Title: A multiplex PCR-based sequencing method for the diagnosis and differentiation of echinococcosis using plasma samples, a proof-of-concept study.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human DNA (NA12878) CORIELL INSTITUTE CatNA12878 Lambda DNA NEB Cat#N3011S Echinococcus gDNA Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China N/A Human plasma samples The People’s Hospital of Ganzi Tibetan Autonomous Prefecture, Kangding, Sichuan Province, China N/A Critical commercial assays Diagnostic Kit for IgG Antibody to Hydatid (ELISA) Haitai Biotech Cat#20153400132 MagPure Circulating DNA KF Kit Magen Cat#MD5432-02 ATOPlex DNA Dual BC Library Prep Set MGI Cat#940-001191-00 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32854 Deposited data Sequencing data This paper CNSA: https://db.cngb.org/cnsa with accession number CNP0005472 Oligonucleotides Primer pairs and targeting genomic positions of the amplicons, see Tables S1 and S2. .. This study and primers from the literature (Wan, Z. et al.20 Spotin, A. et al.,23 Kinkar, L. et al.,24 Toribio, L. et al.25 N/A Software and algorithms ATOPlex Designer MGI https://atoplex.mgi-tech.com/ SOPAnuke v2.1.0 Chen, Y. et al.37 https://github.com/BGI-flexlab/SOAPnuke Fastp v0.23.2 Chen, S. et al.38 https://github.com/OpenGene/fastp Kraken v1.0 Wood, D.E. et al.39 https://ccb.jhu.edu/software/kraken/ Snap-aligner v1.0beta.23 N/A https://github.com/amplab/snap Prinseq v0.20.4 N/A (https://github.com/uwb-linux/prinseq BWA v0.7.19 Li, H. et al.40 https://github.com/lh3/bwa Samtools Danecek, P. et al.41 http://samtools.sourceforge.net/ Bedtools v2.31.0 Quinlan, A.R. et al.42 https://github.com/arq5x/bedtools2 R v4.3.0 R Project https://www.r-project.org/ Stata Stata Corp, College Station, TX, USA https://www.stata.com/ Biorender.com Biorender https://www.biorender.com/ Cell Reports Methods 6, 101428, June 15, 2026 e1 Article ll OPEN ACCESS Biological samples and clinical information collection Lanzhou Veterinary Research Institute provided Echinococcus gDNA.
Sequencing:Article Title: A multiplex PCR-based sequencing method for the diagnosis and differentiation of echinococcosis using plasma samples, a proof-of-concept study.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human DNA (NA12878) CORIELL INSTITUTE CatNA12878 Lambda DNA NEB Cat#N3011S Echinococcus gDNA Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China N/A Human plasma samples The People’s Hospital of Ganzi Tibetan Autonomous Prefecture, Kangding, Sichuan Province, China N/A Critical commercial assays Diagnostic Kit for IgG Antibody to Hydatid (ELISA) Haitai Biotech Cat#20153400132 MagPure Circulating DNA KF Kit Magen Cat#MD5432-02 ATOPlex DNA Dual BC Library Prep Set MGI Cat#940-001191-00 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32854 Deposited data Sequencing data This paper CNSA: https://db.cngb.org/cnsa with accession number CNP0005472 Oligonucleotides Primer pairs and targeting genomic positions of the amplicons, see Tables S1 and S2. .. This study and primers from the literature (Wan, Z. et al.20 Spotin, A. et al.,23 Kinkar, L. et al.,24 Toribio, L. et al.25 N/A Software and algorithms ATOPlex Designer MGI https://atoplex.mgi-tech.com/ SOPAnuke v2.1.0 Chen, Y. et al.37 https://github.com/BGI-flexlab/SOAPnuke Fastp v0.23.2 Chen, S. et al.38 https://github.com/OpenGene/fastp Kraken v1.0 Wood, D.E. et al.39 https://ccb.jhu.edu/software/kraken/ Snap-aligner v1.0beta.23 N/A https://github.com/amplab/snap Prinseq v0.20.4 N/A (https://github.com/uwb-linux/prinseq BWA v0.7.19 Li, H. et al.40 https://github.com/lh3/bwa Samtools Danecek, P. et al.41 http://samtools.sourceforge.net/ Bedtools v2.31.0 Quinlan, A.R. et al.42 https://github.com/arq5x/bedtools2 R v4.3.0 R Project https://www.r-project.org/ Stata Stata Corp, College Station, TX, USA https://www.stata.com/ Biorender.com Biorender https://www.biorender.com/ Cell Reports Methods 6, 101428, June 15, 2026 e1 Article ll OPEN ACCESS Biological samples and clinical information collection Lanzhou Veterinary Research Institute provided Echinococcus gDNA.
other:
Article Title: In‑Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing
Article Snippet: Lambda DNA, pUC19, and phiX174 single-stranded and supercoiled DNA were purchased from New England Biolabs (N3011S, N3041S, N3021S, N3023S).
Methylation:Article Title: Compositions and methods for analyzing modified nucleotides
Article Snippet: .. TABLE 3 Methylation (NEBNext (APOBEC)) and bisulfite Illumina libraries (WGBS) have similar global methylation levels % Methylation (50 ng) % Methylation (10 ng) CpG CHG CHH CpG CHG CHH NA12878 NEBNext 53.7 ± 0.00 0.7 ± 0.07 0.7 ± 0.07 53.7 ± 0.07 0.8 ± 0.07 0.8 ± 0.14 WGBS 53.9 ± 0.42 0.9 ± 0.14 1.2 ± 0.14 52.6 ± 0.07 0.7 ± 0.00 0.9 ± 0.00 Lambda NEBNext 0.6 ± 0.00 0.6 ± 0.00 0.6 ± 0.00 0.7 ± 0.07 0.8 ± 0.07 0.8 ± 0.07 WgBs 0.9 ± 0.30 0.95 ± 0.20 1.1 ± 0.20 0.5 ± 0.00 0.5 ± 0.00 0.7 ± 0.00 The percentage methylation for NA12878 and unmethylated lambda DNA in CpG/CHG/CHH contexts using 50 ng and 10 ng DNA inputs are shown from NEBNext (APOBEC) and WGBS libraries. .. Reads were obtained from Illumina 2×100 base by NovaSeq sequencing.
Marker:Article Title: Serotypes, MIC-Based Antimicrobial Susceptibility, and Genotypic Diversity of Actinobacillus pleuropneumoniae Isolates from Diseased Pigs in Brazil
Article Snippet: Gels were stained for 40 min with SYBR Safe ® (Invitrogen, Carlsbad, CA, USA), and DNA fragments were visualized under UV illumination using a Gel Doc XR ® imaging system (Bio-Rad Laboratories, Hercules, CA, USA). .. Band sizes were estimated using a lambda DNA PFGE marker (New England BioLabs, Ipswich, MA, USA). .. Frequency distributions of serotypes and antimicrobial resistance profiles were analyzed using SPSS v16.0 (SPSS Inc., Chicago, IL, USA).
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