lambda phage dna (New England Biolabs)
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New England Biolabs
lambda phage dna
Lambda Phage Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+phage+dna/DNA+Polymerase+I%2C+Large+(Klenow)+Fragment/pmc12781881-85-10-18
Average 99 stars, based on 1584 article reviews
Lambda Phage Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+phage+dna/DNA+Polymerase+I%2C+Large+(Klenow)+Fragment/pmc12781881-85-10-18
Average 99 stars, based on 1584 article reviews
lambda phage dna - by Bioz Stars,
2026-11
99/100 stars
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Incubation:Article Title: Guanine content in the CTCF motif encodes a vulnerability to persistent, alcohol-induced redistribution Article Snippet: After quenching with 10% Triton X-100 and incubation at 37°C for 15 minutes, DpnII (NEB R0543M, 400 U / reaction) was added for 2 hours to digest chromatin. .. Next, digested chromatin was incubated at 65°C for 20 minutes to inactivate DpnII, after which time restriction fragment overhangs were marked with biotin and repaired by incubation at 37°C for one hour in the presence of fill-in Article Title: Identification and full genome characterization of a novel CRESS DNA virus associated with giant freshwater prawn, Macrobrachium Rosenbergii. Article Snippet: intensive aquaculture farming resulted in the emergence of novel diseases, which become the major obstacles to the sustainable development of M. rosenbergii cultivation [3].. So far, viral pathogens including decapod iridescent virus 1 (DIV1) [4], M. rosenbergii nodavirus and extra small virus (MrNV & XSV) [5, 6], and infectious precocity virus (IPV) [1], have led significant economic losses to the M. rosenbergii yield [3].. DIV1 could cause acute infection with a mass mortality [7]; MrNV and XSV are responsible for the white tail disease in the post-larvae stage of M. rosenbergii with high mortality in certain hatcheries [8]; prawns infected with IPV are associated with sexual precocity induced stunted growth [1]. Article Title: Chromatin interaction landscape of epimorphic regeneration in teleost fish Article Snippet: To enhance the efficiency of T4 DNA ligation, the cell pellet was incubated with 50 μl of premix solution [5 μl of 10× NEBuffer 2.1, 1 μl of 100 mM adenosine triphosphate (ATP), 2.5 μl of 100 mM dithiothreitol, 34 μl of H 2 O, and 2.5 μl of T4 PNK (10 U/μl)] for 15 min at 37°C. .. Then, Article Title: Single-allele nanoscale mapping of regulatory variants. Article Snippet: The remaining reaction was centrifuged, PBS washed and resuspended in DNA ligation buffer (Thermo Fisher Scientific; final concentrations: 40 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 10 mM dithiothreitol and 5 mM ATP) supplemented with dNTPs (final concentration 400 μM each for dATP, dCTP, dGTP and dTTP (Thermo Fisher Scientific, cat. no. R0191)) and EGTA 5 mM. .. T4 polynucleotide kinase PNK (NEB, cat. no. M0201L) and DNA polymerase I Virus:Article Title: HIV-driven virome dysbiosis unveils distinct virome features and inter-viral correlations in blood and respiratory niches. Article Snippet: To selectively concentrate viral particles, we performed enzymatic digestion using a nuclease cocktail (TURBO DNase from Ambion with Cat.NO.AM2239, Benzonase Nuclease from Merck with Cat.NO.70664-3, Baseline-ZERO from Epicentre with Cat.NO.DB0715K and RNase A from Thermo Fisher Scientific with Cat.NO.EN0531), 34μl/pool at 37°C for 60 minutes, effectively eliminating unprotected host and environmental nucleic acids 74. .. Viral nucleic acids were extracted using the QIAamp MinElute Virus Spin Kit. cDNA synthesis used SuperScript III (Invitrogen, 18080093), 1μl/pool and cDNA Synthesis:Article Title: HIV-driven virome dysbiosis unveils distinct virome features and inter-viral correlations in blood and respiratory niches. Article Snippet: To selectively concentrate viral particles, we performed enzymatic digestion using a nuclease cocktail (TURBO DNase from Ambion with Cat.NO.AM2239, Benzonase Nuclease from Merck with Cat.NO.70664-3, Baseline-ZERO from Epicentre with Cat.NO.DB0715K and RNase A from Thermo Fisher Scientific with Cat.NO.EN0531), 34μl/pool at 37°C for 60 minutes, effectively eliminating unprotected host and environmental nucleic acids 74. .. Viral nucleic acids were extracted using the QIAamp MinElute Virus Spin Kit. cDNA synthesis used SuperScript III (Invitrogen, 18080093), 1μl/pool and Sequencing:Article Title: HIV-driven virome dysbiosis unveils distinct virome features and inter-viral correlations in blood and respiratory niches. Article Snippet: To selectively concentrate viral particles, we performed enzymatic digestion using a nuclease cocktail (TURBO DNase from Ambion with Cat.NO.AM2239, Benzonase Nuclease from Merck with Cat.NO.70664-3, Baseline-ZERO from Epicentre with Cat.NO.DB0715K and RNase A from Thermo Fisher Scientific with Cat.NO.EN0531), 34μl/pool at 37°C for 60 minutes, effectively eliminating unprotected host and environmental nucleic acids 74. .. Viral nucleic acids were extracted using the QIAamp MinElute Virus Spin Kit. cDNA synthesis used SuperScript III (Invitrogen, 18080093), 1μl/pool and Polymerase Chain Reaction:Article Title: Pooled CRISPRi screening reveals fungal-specific drug target candidates. Article Snippet: The rising rate of drug-resistant fungal infections and the emergence of intrinsically resistant pathogens pose growing clinical challenges.. Because fungi are closely related to mammals, developing antifungals without toxic off-target effects is difficult.. Targeted gene repression can model drug-mediated inhibition and reveal gene dosage sensitivity, but traditional approaches in the fungal pathogen Candida albicans are labour intensive and low throughput. Concentration Assay:Article Title: Single-allele nanoscale mapping of regulatory variants. Article Snippet: The remaining reaction was centrifuged, PBS washed and resuspended in DNA ligation buffer (Thermo Fisher Scientific; final concentrations: 40 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 10 mM dithiothreitol and 5 mM ATP) supplemented with dNTPs (final concentration 400 μM each for dATP, dCTP, dGTP and dTTP (Thermo Fisher Scientific, cat. no. R0191)) and EGTA 5 mM. .. T4 polynucleotide kinase PNK (NEB, cat. no. M0201L) and DNA polymerase I |