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lambda fix ii svj/129 mouse genomic library  (Agilent technologies)


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    Agilent technologies lambda fix ii svj/129 mouse genomic library
    Lambda Fix Ii Svj/129 Mouse Genomic Library, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+fix+ii+mouse+129/pm10864459-60-8-16
    Average 90 stars, based on 1 article reviews
    lambda fix ii svj/129 mouse genomic library - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Kidney development in cadherin-6 mutants: delayed mesenchyme-to-epithelial conversion and loss of nephrons.
    Article Snippet: Genomic sequences for Cad-6 were isolated from an isogenic Lambda FIX II SVJ/129 mouse genomic library (Stratagene) screened with the first 651 bp of Cad-6 cDNA previously described (Cho et al., 1998).

    Plasmid Preparation:

    Article Title: TSC-22 contributes to hematopoietic precursor cell proliferation and repopulation and is epigenetically silenced in large granular lymphocyte leukemia
    Article Snippet: 26 The antibodies used were as follows: rabbit anti–human TSC-22 polyclonal antibody (a kind gift of Drs Hitoshi Kawamata [Dokkyo University School of Medicine, Tochigi, Japan] and Marco G. Cecchini [University of Bern, Bern, Switzerland]); mouse anti–MYC-tag monoclonal antibody (Cell Signaling Technology, Beverly, MA); goat polyclonal anti–β-ACTIN antibody (Santa Cruz Biotechnology, Santa Cruz, CA). .. Targeting vector for the generation of TSC-22 –deficient mice and genotyping analysis The genomic clone encoding murine TSC-22 was isolated from SVJ129 genomic library (Stratagene, La Jolla, CA) and was mapped by restriction analyses. .. A targeting vector was constructed by ligating a 1.7-kb left arm fragment ( Sac I- Xho I) of TSC-22 , a 1.1-kb TK-Neo-cassette ( Xho I- Bam HI) from a pMC1neo plasmid (Stratagene), an 18-bp adaptor to accommodate incompatible cohesive ends ( Bam HI- Kpn I- Xba I; Xba I is compatible to Spe I), and a 6.3-kb right arm fragment ( Spe I- Nsi I) of TSC-22 .

    Isolation:

    Article Title: TSC-22 contributes to hematopoietic precursor cell proliferation and repopulation and is epigenetically silenced in large granular lymphocyte leukemia
    Article Snippet: 26 The antibodies used were as follows: rabbit anti–human TSC-22 polyclonal antibody (a kind gift of Drs Hitoshi Kawamata [Dokkyo University School of Medicine, Tochigi, Japan] and Marco G. Cecchini [University of Bern, Bern, Switzerland]); mouse anti–MYC-tag monoclonal antibody (Cell Signaling Technology, Beverly, MA); goat polyclonal anti–β-ACTIN antibody (Santa Cruz Biotechnology, Santa Cruz, CA). .. Targeting vector for the generation of TSC-22 –deficient mice and genotyping analysis The genomic clone encoding murine TSC-22 was isolated from SVJ129 genomic library (Stratagene, La Jolla, CA) and was mapped by restriction analyses. .. A targeting vector was constructed by ligating a 1.7-kb left arm fragment ( Sac I- Xho I) of TSC-22 , a 1.1-kb TK-Neo-cassette ( Xho I- Bam HI) from a pMC1neo plasmid (Stratagene), an 18-bp adaptor to accommodate incompatible cohesive ends ( Bam HI- Kpn I- Xba I; Xba I is compatible to Spe I), and a 6.3-kb right arm fragment ( Spe I- Nsi I) of TSC-22 .

    Article Title: Pax3:Fkhr interferes with embryonic Pax3 and Pax7 function: implications for alveolar rhabdomyosarcoma cell of origin
    Article Snippet: .. Isolation of genomic clones A 21-kb genomic clone spanning exon 8 to the 3′-untranslated region of Pax7 was isolated from a λ bacteriophage library of mouse strain SvJ-129 (Stratagene). ..

    Article Title: Alveolar rhabdomyosarcomas in conditional Pax3:Fkhr mice: cooperativity of Ink4a/ARF and Trp53 loss of function
    Article Snippet: .. Genomic clones isolation Genomic DNA clones were isolated from a λ bacteriophage library of mouse strain SvJ-129 (Stratagene). ..

    Clone Assay:

    Article Title: Pax3:Fkhr interferes with embryonic Pax3 and Pax7 function: implications for alveolar rhabdomyosarcoma cell of origin
    Article Snippet: .. Isolation of genomic clones A 21-kb genomic clone spanning exon 8 to the 3′-untranslated region of Pax7 was isolated from a λ bacteriophage library of mouse strain SvJ-129 (Stratagene). ..

    Article Title: Alveolar rhabdomyosarcomas in conditional Pax3:Fkhr mice: cooperativity of Ink4a/ARF and Trp53 loss of function
    Article Snippet: .. Genomic clones isolation Genomic DNA clones were isolated from a λ bacteriophage library of mouse strain SvJ-129 (Stratagene). ..



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