2x dna ligase buffer 20 (New England Biolabs)

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New England Biolabs 2x dna ligase buffer 20
2x Dna Ligase Buffer 20, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33799 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+exonuclease+lambda+exonuclease+reaction+buffer/T4+DNA+Ligase/us11560589-943-11-19
Average 99 stars, based on 33799 article reviews

2x dna ligase buffer 20 - by Bioz Stars, 2026-10

99/100 stars

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Polymerase Chain Reaction:

Article Title: Improvement in the enzymatic efficiency of a zinc metalloprotease through random mutagenesis approach: Studies on the enzymatic and structural properties of the mutant
Article Snippet: .. The chemical constituents of polymerase chain reaction (PCR), such as Tris-HCl, Magnesium Chloride (MgCl 2 ), Manganese Chloride (MnCl 2 ), Dimethyl Sulphoxide (DMSO), and Potassium Chloride (KCl), were ordered from Invitrogen (by Thermo Fischer Scientific). dNTPs, restriction enzymes (BamHI and XhoI), Taq DNA polymerase, Quick CIP (Alkaline Phosphatase Calf Intestinal), and T4 λ-DNA ligase were purchased from New England Biolabs (NEB, USA). ..

Article Title: Negative regulation of hippo-mediated antiviral response targeted WSSV in Penaeus vannamei
Article Snippet: Penaeus vannamei is a vital crustacean species in global aquaculture.. It is challenged by various pathogens, including the devastating White Spot Syndrome Virus (WSSV), which often causes high mortality rates.. Hippo signaling pathway, an evolutionarily conserved mechanism controlling organ development and immune responses, has been implicated in antiviral defense.

Article Title: Piwi regulates the usage of alternative transcription start sites in the Drosophila ovary
Article Snippet: .. The Flag-NLS-Piwi-YK construct was generated by site-directed mutagenesis of Tyrosine (Y) (amino acid 551 of Piwi) codon into Leucine (L) codon and Lysine (K) (amino acid 555 of Piwi) codon into Glutamate (E) codon using high-fidelity PCR amplification (NEB) with a primer pair containing the mutated sequence and then ligation with T4 DNA ligase (NEB). ..

other:

Article Title: Dose-dependent sensitivity of human 3D chromatin to a heart disease-linked transcription factor
Article Snippet: Entire eluate was used for adapter ligation in a 50 μl reaction with 6000 U T4 DNA ligase (New England Biolabs #M0202L), 20 nM annealed and uniquely indexed adapters and 1x T4 DNA ligase buffer with 10 mM ATP (New England Biolabs #B0202S) in nuclease-free water for 2 hours at room temperature, followed by 1x bead:sample AMPureXP purification.

Incubation:

Article Title: Pervasive RNA-binding protein enrichment on TAD boundaries regulates TAD organization
Article Snippet: Samples were then centrifuged at 2500 × g for 5 min. Pelleted nuclei were resuspended in 50 μl of fill-in master mix [3.75 μl of 0.4 mM biotin-14-2′-deoxyadenosine-5′-triphosphate, 1.5 μl of 1 mM 2′-deoxycytidine-5′-triphosphate, 1.5 μl of 1 mM 2′-deoxyguanosine-5′-triphosphate, 1.5 μl of 1 mM 3′-deoxythymidine-5′-triphosphate mix, 2 μl of DNA polymerase I (5 U μl −1 ), Large (Klenow) Fragment and 1× NEBuffer 3.1] to fill in the restriction fragment overhangs and mark the DNA ends with biotin. .. Samples were mixed by pipetting and incubated at 23°C for 4 h. Ligation master mix [398 μl of water, 50 μl of 10× NEB T4 DNA ligase buffer, 1 μl of bovine serum albumin (50 mg ml −1 ), 1 μl of T4 DNA ligase (400 U μl −1 )] was added, and samples were incubated at 16°C for overnight in ThermoMixer C with interval shake. ..

Ligation:

Article Title: inDrops-2: a flexible, versatile and cost-efficient droplet microfluidic approach for high-throughput scRNA-seq of fresh and preserved clinical samples
Article Snippet: .. Then, a master mix containing hydrogel beads carrying DNA stub and ligation mixture was distributed in the same 4 × 96-well plates such that each well would contain 12.5 μl of close-packed hydrogel beads, 1.25 μl of 10 × ligation buffer (NEB, B0202S), 0.3 μl of T4 DNA ligase I (NEB, M0202L), 6.25-μl of double-stranded barcoded oligonucleotides (first set of sub-barcodes) at 200 μM concentration and 4.7 μl of nuclease-free water. ..

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Purification:

Article Title: Dose-dependent sensitivity of human 3D chromatin to a heart disease-linked transcription factor
Article Snippet: For Illumina sequencing library preparation, the entire ChIP sample or 2 μl of each input was used. .. End repair was performed in a 100 μl reaction with 15 U T4 DNA polymerase (New England Biolabs #M0203L), 5 U Klenow fragment DNA polymerase (New England Biolabs, #M0210L), 50 U T4 PNK (New England Biolabs #M0201L), 400 μM dNTP (Promega #U1511) and 1x T4 DNA ligase buffer w 10 mM ATP (New England Biolabs #B0202S) in nuclease-free water for 30 min at room temperature, followed by 1.6x bead:sample AmpureXP purification. .. Entire eluate was used for A-tailing in a 50 μl reaction with 1 mM dATP (New England Biolabs #N0440S), 15 U Klenow 3’>5’ exonuclease (New England Biolabs #M0212L) and 1x NEB buffer 2 in nuclease-free water for 30 min at 37°C, followed by 1.6x bead:sample AMPureXP purification.

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