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10x lambda exonuclease reaction buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs 10x lambda exonuclease reaction buffer
    10x Lambda Exonuclease Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+exonuclease+lambda+exonuclease+reaction+buffer/Lambda+Exonuclease/pm40064876-483-16-21
    Average 98 stars, based on 1133 article reviews
    10x lambda exonuclease reaction buffer - by Bioz Stars, 2026-11
    98/100 stars

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    Amplification:

    Article Title: Heterophilic interactions between cell-surface proteins Teneurin-m and Capricious drive dendrite segregation in the Drosophila olfactory circuit
    Article Snippet: .. cDNA was digested using lambda exonuclease (New England Biolabs) and then amplified for 25 cycles. .. Sequencing libraries were prepared from amplified cDNA, pooled, and quantified using BioAnalyser (Agilent).

    other:

    Article Title: Response of the replisome to araA and araC suggests a mechanism for arabinosyl nucleoside tolerance
    Article Snippet: Lambda Exonuclease , New England Biolabs , M0262.

    Polymerase Chain Reaction:

    Article Title: Selection of DNA aptamers targeting PirAB toxin for potential biosensing applications in acute hepatopancreatic necrosis disease (AHPND)
    Article Snippet: .. The single stranded DNA production was done with λ-exonuclease (NEB) digestion, using 3 μl of enzyme (at 5000 U/ml) per 50 μl of PCR product, incubating 30 minutes at 37 °C, followed by an inactivation of 10 minutes at 80 °C to immediately start the new cycle. ..

    Article Title: Development of µg-scale cell-free production method for gene-size ssDNA fragments
    Article Snippet: .. Input dsDNA was generated via preparative PCR (Q5 DNA polymerase, 5'-phosphorylated primer), purified with AmpliClean magnetic beads, and digested with Lambda exo (NEB). .. ssDNA was purified using Quick-DNA/ RNA MagBeads (Zymo Research) and verified by agarose gel electrophoresis.

    Inactivation:

    Article Title: Selection of DNA aptamers targeting PirAB toxin for potential biosensing applications in acute hepatopancreatic necrosis disease (AHPND)
    Article Snippet: .. The single stranded DNA production was done with λ-exonuclease (NEB) digestion, using 3 μl of enzyme (at 5000 U/ml) per 50 μl of PCR product, incubating 30 minutes at 37 °C, followed by an inactivation of 10 minutes at 80 °C to immediately start the new cycle. ..

    Purification:

    Article Title: A systematic fluorescently tagged protein toolkit for dissecting apical extracellular matrix patterning in C. elegans.
    Article Snippet: This phosphorylation was either performed during primer synthesis (Invitrogen) or in-house using T4 polynucleotide kinase (NEB). .. The phosphorylated strand was degraded using lambda exonuclease (NEB) and purified as previously described [37]. ..

    Article Title: Development of µg-scale cell-free production method for gene-size ssDNA fragments
    Article Snippet: .. Input dsDNA was generated via preparative PCR (Q5 DNA polymerase, 5'-phosphorylated primer), purified with AmpliClean magnetic beads, and digested with Lambda exo (NEB). .. ssDNA was purified using Quick-DNA/ RNA MagBeads (Zymo Research) and verified by agarose gel electrophoresis.

    Generated:

    Article Title: Development of µg-scale cell-free production method for gene-size ssDNA fragments
    Article Snippet: .. Input dsDNA was generated via preparative PCR (Q5 DNA polymerase, 5'-phosphorylated primer), purified with AmpliClean magnetic beads, and digested with Lambda exo (NEB). .. ssDNA was purified using Quick-DNA/ RNA MagBeads (Zymo Research) and verified by agarose gel electrophoresis.

    Magnetic Beads:

    Article Title: Development of µg-scale cell-free production method for gene-size ssDNA fragments
    Article Snippet: .. Input dsDNA was generated via preparative PCR (Q5 DNA polymerase, 5'-phosphorylated primer), purified with AmpliClean magnetic beads, and digested with Lambda exo (NEB). .. ssDNA was purified using Quick-DNA/ RNA MagBeads (Zymo Research) and verified by agarose gel electrophoresis.



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