kb dna substrate (New England Biolabs)

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New England Biolabs kb dna substrate
Kb Dna Substrate, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1840 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+dna/Lambda+DNA/pm42913629-185-2-8
Average 97 stars, based on 1840 article reviews

kb dna substrate - by Bioz Stars, 2026-11

97/100 stars

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Article Title: Biocatalytic synthesis of S-adenosyl-l-methionine analogues for allosteric activation of cystathionine-β-synthase.
Article Snippet: Methylation of lambda DNA by SAM analogues was performed using a commercial kit (M0211S, New England Biolabs).

Generated:

Article Title: Spatial 5mC-seq profiling of embryos and decidua after implantation in mammals.
Article Snippet: DNA methylation is vital for development and diseases, yet no spatial DNA methylation profiling technology has been reported.. Here we developed spatial 5mC-seq (SmC-seq), a microfluidic-based method providing unbiased genome-wide methylome at near single-cell resolution.. Applying SmC-seq to mouse post-implantation development revealed a clear spatial heterogenous pattern of DNA methylation in inner cell mass-derived tissues.

Article Title: CRISPR-mediated gap-based replacement enables site-specific DNA lesion insertion into long DNA and nucleosomes.
Article Snippet: .. To test the efficiency of DNA damage insertion, a 1036- bp DNA fragment was generated via PCR amplification using λ-DNA (New England Biolabs, N3011S) as a template with primers (1036F and 1036R) ( Supplementary Table S1 ). ..

Lambda DNA Preparation:

Article Title: Nuclease-NTPase antiphage defence systems use conserved molecular features to control bacterial immunity.
Article Snippet: Bacteria encode diverse defence systems, including restriction– modification and CRISPR–Cas, that cleave nucleic acid to protect against phage infection.. Bioinformatic analyses demonstrate that many recently identified antiphage defence operons comprise a nuclease and NTPase protein, suggesting that additional nucleic acid-targeting systems remain to be understood.. Here we develop large-scale comparative cell biology and biochemical approaches to analyse 16 nuclease–NTPase systems and define molecular features that control antiphage defence.

Article Title: A multiplex PCR-based sequencing method for the diagnosis and differentiation of echinococcosis using plasma samples, a proof-of-concept study.
Article Snippet: .. The panel used Lambda DNA as a spike-in control (NEB, N3011S) with 1,000 copies/reaction (5 primer pairs), and human genomic DNA from cell line (GM12878) as an internal quality control (1 ng/reaction) (7 primer pairs). .. Amplicon library preparation and sequencing E. granulosus and E. multilocularis gDNA were gradient-diluted to a range from 0.1 ng to 0.1 fg using nuclease-free water (Invitrogen, cat #AM9930) in a 10-fold gradient.

Polymerase Chain Reaction:

Amplification:

Control:

Force Spectroscopy:

Article Title: Conjugated Polymeric Nanostructures as Potential Tools in DNA Nanotechnology: Interactions with the Double Helix
Article Snippet: .. The samples prepared for the single-molecule force spectroscopy assays consist of biotin-labeled λ-DNA (48,502 base pairs, New England Biolabs) attached at the ends to a streptavidin-coated polystyrene bead with a 3 μm diameter (Bangs Laboratories) and to a streptavidin-coated glass coverslip (which serves as the bottom surface of the sample chamber used). ..

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