Review



laemmli buffer  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Thermo Fisher laemmli buffer
    Laemmli Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/laemmli+sample+buffer/Laemmli+SDS+sample+buffer%2C+reducing/pm42360315-82-32-34
    Average 99 stars, based on 1 article reviews
    laemmli buffer - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Bicinchoninic Acid Protein Assay:

    Article Title: Bispecific antibody-drug conjugates targeting EGFR and LGR5 exert potent antitumor activity in colorectal cancer models
    Article Snippet: .. Protein concentrations were quantified by BCA protein assay (Thermo Scientific) and lysates were diluted in reducing SDS Laemmli buffer (Thermo Scientific) and incubated at 37°C for 1 h prior to SDS-PAGE. .. Anti-mouse IgG and anti-rabbit IgG HRP-labeled secondary antibodies from Cell Signaling Technology were utilized with the standard ECL protocol (Cytiva).

    Article Title: Neurodegenerative NMNAT2 Deficiency Promotes APP Processing in a SARM1-Dependent Manner.
    Article Snippet: Neurons were lysed using 150 μL of RIPA buffer per well [20 mM of Tris-base, 150 mM of NaCl, 2 mM EDTA, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), 1× cOmpleteTM Mini protease inhibitor cocktail (Roche®, Basel, Switzerland), and phosphatase inhibitor cocktail 3 (Sigma-Aldrich® P0044, St. Louis, MO, USA), pH 7.4] and homogenized for 15 s with a pestle motor using disposable pellet pestles. .. Samples were then sonicated using a Branson Sonifier® 250 (Emerson, St. Louis, MO, USA) for 10 intervals set at duty cycle 30/output 3 and stored at −80 ◦C. https://doi.org/10.3390/cells15121100 Protein concentration was measured using the PierceTM BCA Protein Assay Kit (Thermo ScientificTM, Waltham, MA, USA) and samples were prepared for loading in Laemmli SDS buffer (Thermo ScientificTM). ..

    Incubation:

    Article Title: Bispecific antibody-drug conjugates targeting EGFR and LGR5 exert potent antitumor activity in colorectal cancer models
    Article Snippet: .. Protein concentrations were quantified by BCA protein assay (Thermo Scientific) and lysates were diluted in reducing SDS Laemmli buffer (Thermo Scientific) and incubated at 37°C for 1 h prior to SDS-PAGE. .. Anti-mouse IgG and anti-rabbit IgG HRP-labeled secondary antibodies from Cell Signaling Technology were utilized with the standard ECL protocol (Cytiva).

    Sonication:

    Article Title: Neurodegenerative NMNAT2 Deficiency Promotes APP Processing in a SARM1-Dependent Manner.
    Article Snippet: Neurons were lysed using 150 μL of RIPA buffer per well [20 mM of Tris-base, 150 mM of NaCl, 2 mM EDTA, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), 1× cOmpleteTM Mini protease inhibitor cocktail (Roche®, Basel, Switzerland), and phosphatase inhibitor cocktail 3 (Sigma-Aldrich® P0044, St. Louis, MO, USA), pH 7.4] and homogenized for 15 s with a pestle motor using disposable pellet pestles. .. Samples were then sonicated using a Branson Sonifier® 250 (Emerson, St. Louis, MO, USA) for 10 intervals set at duty cycle 30/output 3 and stored at −80 ◦C. https://doi.org/10.3390/cells15121100 Protein concentration was measured using the PierceTM BCA Protein Assay Kit (Thermo ScientificTM, Waltham, MA, USA) and samples were prepared for loading in Laemmli SDS buffer (Thermo ScientificTM). ..

    Protein Concentration:

    Article Title: Neurodegenerative NMNAT2 Deficiency Promotes APP Processing in a SARM1-Dependent Manner.
    Article Snippet: Neurons were lysed using 150 μL of RIPA buffer per well [20 mM of Tris-base, 150 mM of NaCl, 2 mM EDTA, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), 1× cOmpleteTM Mini protease inhibitor cocktail (Roche®, Basel, Switzerland), and phosphatase inhibitor cocktail 3 (Sigma-Aldrich® P0044, St. Louis, MO, USA), pH 7.4] and homogenized for 15 s with a pestle motor using disposable pellet pestles. .. Samples were then sonicated using a Branson Sonifier® 250 (Emerson, St. Louis, MO, USA) for 10 intervals set at duty cycle 30/output 3 and stored at −80 ◦C. https://doi.org/10.3390/cells15121100 Protein concentration was measured using the PierceTM BCA Protein Assay Kit (Thermo ScientificTM, Waltham, MA, USA) and samples were prepared for loading in Laemmli SDS buffer (Thermo ScientificTM). ..

    Western Blot:

    Article Title: MAPK/ERK signaling regulates H3K9me3 heterochromatin reorganization to confer mesendoderm developmental competence.
    Article Snippet: Protein concentration was measured using the Pierce icinchoninic acid (BCA) protein assay kit (23 225, Thermo isher Scientific). .. For western blot analysis, 5–15 μg of whole cell lysate in Laemmli Sample Buffer (J60015, Alfa Aesar) was denatured at 100◦C for 5 min, then separated on a 10%–15% sodium dodecyl sulphate–polyacrylamide gel electrophoresis gel at 60 V for 30 min, followed by 100 V for 2–2.5 h. Proteins on the gel were transferred to a 0.2 μm pore size nitrocellulose membrane (1620 112, Bio-Rad) using a wet tank transfer system with the Mini Blot Module (Thermo Fisher Scientific) at 10 V for 1 h. The transferred membrane was briefly washed with tween tris-buffered saline (TTBS) (0.05% Tween-20 in TBS) and blocked with blocking buffer [5% Blotting-Grade Blocker (1706 404, Bio-Rad) in TBS] for 1 h at room temperature. .. maging data processing enoising: Acquired images were denoised using the “Deoise.ai” function in NIS Elements Advanced Research (verion 5.20/5.30/5.42, Nikon Instrument).

    Nucleic Acid Electrophoresis:

    Article Title: MAPK/ERK signaling regulates H3K9me3 heterochromatin reorganization to confer mesendoderm developmental competence.
    Article Snippet: Protein concentration was measured using the Pierce icinchoninic acid (BCA) protein assay kit (23 225, Thermo isher Scientific). .. For western blot analysis, 5–15 μg of whole cell lysate in Laemmli Sample Buffer (J60015, Alfa Aesar) was denatured at 100◦C for 5 min, then separated on a 10%–15% sodium dodecyl sulphate–polyacrylamide gel electrophoresis gel at 60 V for 30 min, followed by 100 V for 2–2.5 h. Proteins on the gel were transferred to a 0.2 μm pore size nitrocellulose membrane (1620 112, Bio-Rad) using a wet tank transfer system with the Mini Blot Module (Thermo Fisher Scientific) at 10 V for 1 h. The transferred membrane was briefly washed with tween tris-buffered saline (TTBS) (0.05% Tween-20 in TBS) and blocked with blocking buffer [5% Blotting-Grade Blocker (1706 404, Bio-Rad) in TBS] for 1 h at room temperature. .. maging data processing enoising: Acquired images were denoised using the “Deoise.ai” function in NIS Elements Advanced Research (verion 5.20/5.30/5.42, Nikon Instrument).

    Pore Size:

    Article Title: MAPK/ERK signaling regulates H3K9me3 heterochromatin reorganization to confer mesendoderm developmental competence.
    Article Snippet: Protein concentration was measured using the Pierce icinchoninic acid (BCA) protein assay kit (23 225, Thermo isher Scientific). .. For western blot analysis, 5–15 μg of whole cell lysate in Laemmli Sample Buffer (J60015, Alfa Aesar) was denatured at 100◦C for 5 min, then separated on a 10%–15% sodium dodecyl sulphate–polyacrylamide gel electrophoresis gel at 60 V for 30 min, followed by 100 V for 2–2.5 h. Proteins on the gel were transferred to a 0.2 μm pore size nitrocellulose membrane (1620 112, Bio-Rad) using a wet tank transfer system with the Mini Blot Module (Thermo Fisher Scientific) at 10 V for 1 h. The transferred membrane was briefly washed with tween tris-buffered saline (TTBS) (0.05% Tween-20 in TBS) and blocked with blocking buffer [5% Blotting-Grade Blocker (1706 404, Bio-Rad) in TBS] for 1 h at room temperature. .. maging data processing enoising: Acquired images were denoised using the “Deoise.ai” function in NIS Elements Advanced Research (verion 5.20/5.30/5.42, Nikon Instrument).

    Membrane:

    Article Title: MAPK/ERK signaling regulates H3K9me3 heterochromatin reorganization to confer mesendoderm developmental competence.
    Article Snippet: Protein concentration was measured using the Pierce icinchoninic acid (BCA) protein assay kit (23 225, Thermo isher Scientific). .. For western blot analysis, 5–15 μg of whole cell lysate in Laemmli Sample Buffer (J60015, Alfa Aesar) was denatured at 100◦C for 5 min, then separated on a 10%–15% sodium dodecyl sulphate–polyacrylamide gel electrophoresis gel at 60 V for 30 min, followed by 100 V for 2–2.5 h. Proteins on the gel were transferred to a 0.2 μm pore size nitrocellulose membrane (1620 112, Bio-Rad) using a wet tank transfer system with the Mini Blot Module (Thermo Fisher Scientific) at 10 V for 1 h. The transferred membrane was briefly washed with tween tris-buffered saline (TTBS) (0.05% Tween-20 in TBS) and blocked with blocking buffer [5% Blotting-Grade Blocker (1706 404, Bio-Rad) in TBS] for 1 h at room temperature. .. maging data processing enoising: Acquired images were denoised using the “Deoise.ai” function in NIS Elements Advanced Research (verion 5.20/5.30/5.42, Nikon Instrument).

    Saline:

    Article Title: MAPK/ERK signaling regulates H3K9me3 heterochromatin reorganization to confer mesendoderm developmental competence.
    Article Snippet: Protein concentration was measured using the Pierce icinchoninic acid (BCA) protein assay kit (23 225, Thermo isher Scientific). .. For western blot analysis, 5–15 μg of whole cell lysate in Laemmli Sample Buffer (J60015, Alfa Aesar) was denatured at 100◦C for 5 min, then separated on a 10%–15% sodium dodecyl sulphate–polyacrylamide gel electrophoresis gel at 60 V for 30 min, followed by 100 V for 2–2.5 h. Proteins on the gel were transferred to a 0.2 μm pore size nitrocellulose membrane (1620 112, Bio-Rad) using a wet tank transfer system with the Mini Blot Module (Thermo Fisher Scientific) at 10 V for 1 h. The transferred membrane was briefly washed with tween tris-buffered saline (TTBS) (0.05% Tween-20 in TBS) and blocked with blocking buffer [5% Blotting-Grade Blocker (1706 404, Bio-Rad) in TBS] for 1 h at room temperature. .. maging data processing enoising: Acquired images were denoised using the “Deoise.ai” function in NIS Elements Advanced Research (verion 5.20/5.30/5.42, Nikon Instrument).

    Blocking Assay:

    Article Title: MAPK/ERK signaling regulates H3K9me3 heterochromatin reorganization to confer mesendoderm developmental competence.
    Article Snippet: Protein concentration was measured using the Pierce icinchoninic acid (BCA) protein assay kit (23 225, Thermo isher Scientific). .. For western blot analysis, 5–15 μg of whole cell lysate in Laemmli Sample Buffer (J60015, Alfa Aesar) was denatured at 100◦C for 5 min, then separated on a 10%–15% sodium dodecyl sulphate–polyacrylamide gel electrophoresis gel at 60 V for 30 min, followed by 100 V for 2–2.5 h. Proteins on the gel were transferred to a 0.2 μm pore size nitrocellulose membrane (1620 112, Bio-Rad) using a wet tank transfer system with the Mini Blot Module (Thermo Fisher Scientific) at 10 V for 1 h. The transferred membrane was briefly washed with tween tris-buffered saline (TTBS) (0.05% Tween-20 in TBS) and blocked with blocking buffer [5% Blotting-Grade Blocker (1706 404, Bio-Rad) in TBS] for 1 h at room temperature. .. maging data processing enoising: Acquired images were denoised using the “Deoise.ai” function in NIS Elements Advanced Research (verion 5.20/5.30/5.42, Nikon Instrument).

    SDS Page:

    Article Title: Physicochemical stability and protein corona profiling on the interaction of iron oxide nanoparticles with human tears.
    Article Snippet: .. SDS-PAGE of tear extract controls and unbound or loosely bound (soft corona) proteins and the IONP extracted proteins, 10 mL of each protein samples were mixed separately with 10 mL of 2 Laemmli buffer (Invitrogen) and heated at 95 1C for 5 minutes to ensure complete protein denaturation. .. The prepared samples, along with 2 mL of Benchmarkt pre-stained protein molecular weight ladder (Invitrogen), were loaded onto a 12% Tris-polyacrylamide gel and electrophoresed in Trisglycine running buffer (Invitrogen) at 120 V for 45 minutes.

    Protease Inhibitor:

    Article Title: Immortalization of human airway epithelial cells for genome editing applications
    Article Snippet: .. Differentiated cells were trypsinized and lysed in Laemmli buffer (120 mM Tris-HCl pH 6.8, 20 % glycerol, 4 % sodium dodecyl sulfate (SDS), protease inhibitor (Fisher Emergo B.V.)), and the lysates were homogenized using a QIAshredder (QIAGEN N.V., Germany) column to reduce viscosity. .. Protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, MA, USA) according to the manufacturer’s instructions.

    Viscosity:

    Article Title: Immortalization of human airway epithelial cells for genome editing applications
    Article Snippet: .. Differentiated cells were trypsinized and lysed in Laemmli buffer (120 mM Tris-HCl pH 6.8, 20 % glycerol, 4 % sodium dodecyl sulfate (SDS), protease inhibitor (Fisher Emergo B.V.)), and the lysates were homogenized using a QIAshredder (QIAGEN N.V., Germany) column to reduce viscosity. .. Protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, MA, USA) according to the manufacturer’s instructions.

    Isolation:

    Article Title: The club cell circadian clock regulates temporal patterns in leukocyte trafficking in chronic allergic airways disease
    Article Snippet: Mouse lungs were weighed and approximately 30–35 mg of tissue was lysed using 0.5 ml T-Per tissue protein extraction reagent (Thermo Scientific) supplemented with protease and phosphatase inhibitor cocktail (cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail, MilliporeSigma) using a lysing matrix D on a homogenizer. .. Proteins were isolated from the lysate supernatant and quantified using Pierce BCA assay, and 50 μg of protein was heated at 95 °C for 5 min in Laemmli SDS sample reducing buffer (Thermo Scientific) and loaded into Any kD Mini-PROTEAN TGX Precast Protein Gels (BioRad). .. Gels were run using premixed electrophoresis buffer (BioRad) at 100 V for an hour.

    BIA-KA:

    Article Title: The club cell circadian clock regulates temporal patterns in leukocyte trafficking in chronic allergic airways disease
    Article Snippet: Mouse lungs were weighed and approximately 30–35 mg of tissue was lysed using 0.5 ml T-Per tissue protein extraction reagent (Thermo Scientific) supplemented with protease and phosphatase inhibitor cocktail (cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail, MilliporeSigma) using a lysing matrix D on a homogenizer. .. Proteins were isolated from the lysate supernatant and quantified using Pierce BCA assay, and 50 μg of protein was heated at 95 °C for 5 min in Laemmli SDS sample reducing buffer (Thermo Scientific) and loaded into Any kD Mini-PROTEAN TGX Precast Protein Gels (BioRad). .. Gels were run using premixed electrophoresis buffer (BioRad) at 100 V for an hour.



    Similar Products

    laemmli buffer  (Boston BioProducts)
    97
    Boston BioProducts laemmli buffer
    Laemmli Buffer, supplied by Boston BioProducts, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/laemmli+sample+buffer/Laemmli+SDS-Sample+Buffer/pmc13675307-110-13-14
    Average 97 stars, based on 1 article reviews
    laemmli buffer - by Bioz Stars, 2026-10
    97/100 stars
    		  Buy from Supplier
    laemmli buffer  (Bio-Rad)
    97
    Bio-Rad laemmli buffer
    Laemmli Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/laemmli+sample+buffer/2x+Laemmli+Sample+Buffer/pm42898112-258-18-20
    Average 97 stars, based on 1 article reviews
    laemmli buffer - by Bioz Stars, 2026-10
    97/100 stars
    		  Buy from Supplier
    4x laemmli sample buffer bio rad  (Bio-Rad)
    99
    Bio-Rad 4x laemmli sample buffer bio rad
    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, <t>Bio‐Rad)</t> are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.
    4x Laemmli Sample Buffer Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/laemmli+sample+buffer/4x+Laemmli+Sample+Buffer/pmc13675934-66-17-21
    Average 99 stars, based on 1 article reviews
    4x laemmli sample buffer bio rad - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    1x laemmli sample buffer  (Bio-Rad)
    99
    Bio-Rad 1x laemmli sample buffer
    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, <t>Bio‐Rad)</t> are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.
    1x Laemmli Sample Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/laemmli+sample+buffer/4x+Laemmli+Sample+Buffer/pm42889706-1016-16-20
    Average 99 stars, based on 1 article reviews
    1x laemmli sample buffer - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    laemmli sample buffer  (Bio-Rad)
    99
    Bio-Rad laemmli sample buffer
    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, <t>Bio‐Rad)</t> are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.
    Laemmli Sample Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/laemmli+sample+buffer/4x+Laemmli+Sample+Buffer/pm42883798-169-13-17
    Average 99 stars, based on 1 article reviews
    laemmli sample buffer - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    4x laemmli sample buffer  (Bio-Rad)
    99
    Bio-Rad 4x laemmli sample buffer
    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, <t>Bio‐Rad)</t> are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.
    4x Laemmli Sample Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/laemmli+sample+buffer/4x+Laemmli+Sample+Buffer/pm42886259-33-4-27
    Average 99 stars, based on 1 article reviews
    4x laemmli sample buffer - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    protein concentrations  (Bio-Rad)
    99
    Bio-Rad protein concentrations
    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, <t>Bio‐Rad)</t> are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.
    Protein Concentrations, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/laemmli+sample+buffer/4x+Laemmli+Sample+Buffer/pm42886558-335-3-14
    Average 99 stars, based on 1 article reviews
    protein concentrations - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    sample buffer  (Bio-Rad)
    99
    Bio-Rad sample buffer
    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, <t>Bio‐Rad)</t> are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.
    Sample Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/laemmli+sample+buffer/4x+Laemmli+Sample+Buffer/pm42882515-76-8-11
    Average 99 stars, based on 1 article reviews
    sample buffer - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, Bio‐Rad) are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.

    Journal: The FASEB Journal

    Article Title: Elucidating the Molecular Impact of DA29‐Induced ClpP Chemoactivation on Mitochondrial Function

    doi: 10.1096/fj.202603398RR

    Figure Lengend Snippet: Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, Bio‐Rad) are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.

    Article Snippet: Whole‐cell proteins (20‐30 μg), mitochondrial lysates (30 μg) or sucrose gradient fractions (10 μL) were denatured in 4X Laemmli sample buffer (Bio‐Rad) complemented with 10% 2‐mercaptoethanol, separated by SDS‐PAGE and subjected to immunoblotting as described in [ ], using the following primary antibodies: ClpP (#A306224, Antibodies.com), ClpX (#ab168338, Abcam), total OXPHOS cocktail (#ab110413, Abcam), HA‐tag (#3724, Cell Signaling Technology), uL13m (#16241–1‐AP, Proteintech), VDAC1 (#ab186321, Abcam), mS29 (# MA141279 , Thermo Fisher Scientific), α‐tubulin (#T6074, Sigma‐Aldrich) and β‐actin (#A2066, Sigma‐Aldrich).

    Techniques: Activation Assay, Western Blot, Control, Analysis, Gradient Centrifugation, Migration, Marker, Fluorescence, Activity Assay, Incubation, Concentration Assay, Molecular Weight, Staining, Negative Control