laemli buffer (Applichem inc)

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Applichem inc laemli buffer
Laemli Buffer, supplied by Applichem inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/laemlli+loading+buffer/laemmli+buffer/pmc02656543-66-9-11
Average 90 stars, based on 1 article reviews

laemli buffer - by Bioz Stars, 2026-09

90/100 stars

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Article Title: Acid sphingomyelinase deactivation post-ischemia promotes brain angiogenesis and remodeling by small extracellular vesicles
Article Snippet: For Western blot, 30 μg protein samples were solubilised with Laemmli sample buffer under reducing (containing dithiothreitol [DTT]; AppliChem, Darmstadt, Germany) or non-reducing (not containing DTT) conditions and separated on SDS–PAGE gels before transfer to polyvinylidene fluoride membranes (PVDF; Millipore, Darmstadt, Germany).

Article Title: Functionalized Terpolymer-Brush-Based Biointerface with Improved Antifouling Properties for Ultra-Sensitive Direct Detection of Virus in Crude Clinical Samples.
Article Snippet: New analytical techniques that overcome major drawbacks of current routinely used viral infection diagnosis methods, i.e., the long analysis time and laboriousness of real-time reversetranscription polymerase chain reaction (qRT-PCR) and the insufficient sensitivity of “antigen tests”, are urgently needed in the context of SARS-CoV-2 and other highly contagious viruses.. Here, we report on an antifouling terpolymer-brush biointerface that enables the rapid and sensitive detection of SARSCoV-2 in untreated clinical samples.. The developed biointerface carries a tailored composition of zwitterionic and non-ionic moieties and allows for the significant improvement of antifouling capabilities when postmodified with biorecognition elements and exposed to complex media.

Article Title: Cathepsin S Is More Abundant in Serum of Mycobacterium avium subsp. paratuberculosis -Infected Dairy Cows
Article Snippet: To 0.5 μL serum 1× Laemmli-buffer (50 mM tris-HCl, 1% SDS (AppliChem, Darmstadt, Germany), 10% glycerol (SERVA, Heidelberg, Germany), 4 mM 2-mercaptoethanol (Sigma-Aldrich, Taufkirchen, Germany), bromophenol blue (Sigma-Aldrich)) was added.

Article Title: Cathepsin S Is More Abundant in Serum of Mycobacterium avium subsp. paratuberculosis -Infected Dairy Cows.
Article Snippet: To 0.5 μL serum 1× Laemmli-buffer (50 mM tris-HCl, 1% SDS (AppliChem, Darmstadt, Germany), 10% glycerol (SERVA, Heidelberg, Germany), 4 mM 2-mercaptoethanol (SigmaAldrich, Taufkirchen, Germany), bromophenol blue (Sigma-Aldrich)) was added.

Protease Inhibitor:

Article Title: Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells
Article Snippet: .. Briefly, dissected embryos at E9.5 were lysed in Laemmli buffer, and the cells were lysed in RIPA buffer 150 mM NaCl, 50 mM Tris pH 7.6, 1% Triton X-100, 0.25% sodium deoxycholate, and 0.1% SDS (AppliChem) with an ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail (Merck), phosphatase inhibitor cocktail sets II and IV (Merck), and phenylmethylsulfonyl fluoride (PMSF; Sigma-Aldrich). ..

Nucleic Acid Electrophoresis:

Article Title: EV products obtained from iPSC-derived MSCs show batch-to-batch variations in their ability to modulate allogeneic immune responses in vitro
Article Snippet: .. Briefly, 5 μL of iMSC-EV preparations were solved and denatured using Laemmli sample buffer (4×) with dithiothreitol (AppliChem GmbH, Darmstadt, Germany) and separated by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis on 12% gels. .. Proteins were transferred to polyvinylidene fluoride membranes (Sigma-Aldrich), which were subsequently blocked with Tris-buffered saline and 0.1% Tween 20 containing 5% (w/v) skim milk powder (Sigma-Aldrich).

Polyacrylamide Gel Electrophoresis:

Article Title: CD73 activity of mesenchymal stromal cell-derived extracellular vesicle preparations is detergent-resistant and does not correlate with immunomodulatory capabilities.
Article Snippet: Background aims: Extracellular vesicles (EVs) derived from human mesenchymal stromal cells (MSCs) show immunomodulatory activity in different assays both in vitro and in vivo.. In previous work, the authors compared the immunomodulatory potential of independent MSC-EV preparations in a multi-donor mixed lymphocyte reaction (mdMLR) assay and an optimized steroid-refractory acute graft-versus-host disease (aGVHD) mouse model.. The authors observed that only a proportion of the MSC-EV preparations showed immunomodulatory capabilities and demonstrated that only MSC-EV preparations with mdMLR immunomodulating activities were able to suppress aGVHD symptoms in vivo and vice versa.

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