Review



krt14  (Novus Biologicals)


Bioz Verified Symbol Novus Biologicals is a verified supplier
Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Novus Biologicals krt14
    Characterization of the cultured hiPSCs derived ITGB4 + corneal cells. A Differentiation of ocular cells from hiPSCs, the representative image showed the various ocular cells, within optic vesicle and the epithelial cells area (black box). Scale bar: 200 μm. B The representative image showed the unsorted epithelial and mesenchymal cells after eliminating the neural cells. C FACS sorted ITGB4 + corneal progenitor cells from hiPSCs derived ocular cells. D Representative images of cultured ITGB4 + corneal progenitor cells. Scale bars: 50 μm. E The single cells clone forming capacity of ITGB4 + corneal progenitor cell. Scale bar: 10 μm. F Immunofluorescent staining of ITGB4 + corneal progenitor cells with ITGB4 (green), <t>Krt14</t> (red), Krt12 (red), P63 (red), PAX6 (green) and Ki67(green). Scale bars: 100 μm. G The representative image of ITGB4 + corneal progenitor cell spheres. Scale bars: 200 μm. H Immunofluorescent staining of ITGB4 + corneal progenitor cell spheres with ITGB4, Krt14, Krt12 and PAX6. Scale bar: 100 μm. I , J The q-RT PCR results show the genes expression by GAPDH normalized △CT value and fold change
    Krt14, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/krt14/Cytokeratin+14+Antibody+(KRT14%2F532)/pmc12837101-6-0-4
    Average 93 stars, based on 2 article reviews
    krt14 - by Bioz Stars, 2026-11
    93/100 stars

    Images

    1) Product Images from "Simultaneous generation of transplantable RGC-like and corneal progenitor cells from hiPSCs using a dual-lineage platform"

    Article Title: Simultaneous generation of transplantable RGC-like and corneal progenitor cells from hiPSCs using a dual-lineage platform

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/s13287-025-04843-z

    Characterization of the cultured hiPSCs derived ITGB4 + corneal cells. A Differentiation of ocular cells from hiPSCs, the representative image showed the various ocular cells, within optic vesicle and the epithelial cells area (black box). Scale bar: 200 μm. B The representative image showed the unsorted epithelial and mesenchymal cells after eliminating the neural cells. C FACS sorted ITGB4 + corneal progenitor cells from hiPSCs derived ocular cells. D Representative images of cultured ITGB4 + corneal progenitor cells. Scale bars: 50 μm. E The single cells clone forming capacity of ITGB4 + corneal progenitor cell. Scale bar: 10 μm. F Immunofluorescent staining of ITGB4 + corneal progenitor cells with ITGB4 (green), Krt14 (red), Krt12 (red), P63 (red), PAX6 (green) and Ki67(green). Scale bars: 100 μm. G The representative image of ITGB4 + corneal progenitor cell spheres. Scale bars: 200 μm. H Immunofluorescent staining of ITGB4 + corneal progenitor cell spheres with ITGB4, Krt14, Krt12 and PAX6. Scale bar: 100 μm. I , J The q-RT PCR results show the genes expression by GAPDH normalized △CT value and fold change
    Figure Legend Snippet: Characterization of the cultured hiPSCs derived ITGB4 + corneal cells. A Differentiation of ocular cells from hiPSCs, the representative image showed the various ocular cells, within optic vesicle and the epithelial cells area (black box). Scale bar: 200 μm. B The representative image showed the unsorted epithelial and mesenchymal cells after eliminating the neural cells. C FACS sorted ITGB4 + corneal progenitor cells from hiPSCs derived ocular cells. D Representative images of cultured ITGB4 + corneal progenitor cells. Scale bars: 50 μm. E The single cells clone forming capacity of ITGB4 + corneal progenitor cell. Scale bar: 10 μm. F Immunofluorescent staining of ITGB4 + corneal progenitor cells with ITGB4 (green), Krt14 (red), Krt12 (red), P63 (red), PAX6 (green) and Ki67(green). Scale bars: 100 μm. G The representative image of ITGB4 + corneal progenitor cell spheres. Scale bars: 200 μm. H Immunofluorescent staining of ITGB4 + corneal progenitor cell spheres with ITGB4, Krt14, Krt12 and PAX6. Scale bar: 100 μm. I , J The q-RT PCR results show the genes expression by GAPDH normalized △CT value and fold change

    Techniques Used: Cell Culture, Derivative Assay, Staining, Reverse Transcription Polymerase Chain Reaction, Expressing

    Generation of hiPSCs derived corneal organoids. A The representative image of hiPSCs derived corneal organoids with transparent property. scale bars: 200 μm. B , C Immunofluorescent staining of corneal organoid sections with ITGB4 (green), Krt14 (red), Krt12 (red) and P63 (red). Scale bars: 100 μm and 50 μm, respectively
    Figure Legend Snippet: Generation of hiPSCs derived corneal organoids. A The representative image of hiPSCs derived corneal organoids with transparent property. scale bars: 200 μm. B , C Immunofluorescent staining of corneal organoid sections with ITGB4 (green), Krt14 (red), Krt12 (red) and P63 (red). Scale bars: 100 μm and 50 μm, respectively

    Techniques Used: Derivative Assay, Staining

    Related Articles

    In Vitro:

    Article Title: Framework nucleic acids as programmable carrier for transdermal drug delivery
    Article Snippet: .. Such sample size was chosen with referral to similar studies previously Data exclusions No data were excluded from the analyses, except for outliers as determined with Tukey's fences Replication All in vitro analyses were repeated at least three times with n>=3; all in vivo analyses were repeated at least twice Randomization Animals, ex vivo tissues were allocated randomly to different treatment groups Blinding Single blinding was performed for all animal studies, where personnel in charge of data collection and analyses is not aware of treatment conditions Reporting for specific materials, systems and methods Materials & experimental systems n/a Involved in the study Unique biological materials Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Unique biological materials Policy information about availability of materials Obtaining unique materials All unique materials used are available from the authors or from standard commercial sources (Sigma-Aldrich, Lonza, Gibco Thermofisher) Antibodies Antibodies used mouse Cytokeratin14 antibody (KRT14/532) [DyLight 488] from Novus Biologicals Validation Novusbio guarantees primary and secondary antibodies of homology >90% within one year from receipt. .. Authors validated and optimized Antibody concentration with signal on dermis region Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Males, 6 week-old NCR nude mice or C57Bl6 mice were obtained from InVivos Singapore, used within 2 weeks of delivery Wild animals No wild animals were used in this study Field-collected samples This study does not involve field collection of samples

    In Vivo:

    Article Title: Framework nucleic acids as programmable carrier for transdermal drug delivery
    Article Snippet: .. Such sample size was chosen with referral to similar studies previously Data exclusions No data were excluded from the analyses, except for outliers as determined with Tukey's fences Replication All in vitro analyses were repeated at least three times with n>=3; all in vivo analyses were repeated at least twice Randomization Animals, ex vivo tissues were allocated randomly to different treatment groups Blinding Single blinding was performed for all animal studies, where personnel in charge of data collection and analyses is not aware of treatment conditions Reporting for specific materials, systems and methods Materials & experimental systems n/a Involved in the study Unique biological materials Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Unique biological materials Policy information about availability of materials Obtaining unique materials All unique materials used are available from the authors or from standard commercial sources (Sigma-Aldrich, Lonza, Gibco Thermofisher) Antibodies Antibodies used mouse Cytokeratin14 antibody (KRT14/532) [DyLight 488] from Novus Biologicals Validation Novusbio guarantees primary and secondary antibodies of homology >90% within one year from receipt. .. Authors validated and optimized Antibody concentration with signal on dermis region Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Males, 6 week-old NCR nude mice or C57Bl6 mice were obtained from InVivos Singapore, used within 2 weeks of delivery Wild animals No wild animals were used in this study Field-collected samples This study does not involve field collection of samples

    Ex Vivo:

    Article Title: Framework nucleic acids as programmable carrier for transdermal drug delivery
    Article Snippet: .. Such sample size was chosen with referral to similar studies previously Data exclusions No data were excluded from the analyses, except for outliers as determined with Tukey's fences Replication All in vitro analyses were repeated at least three times with n>=3; all in vivo analyses were repeated at least twice Randomization Animals, ex vivo tissues were allocated randomly to different treatment groups Blinding Single blinding was performed for all animal studies, where personnel in charge of data collection and analyses is not aware of treatment conditions Reporting for specific materials, systems and methods Materials & experimental systems n/a Involved in the study Unique biological materials Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Unique biological materials Policy information about availability of materials Obtaining unique materials All unique materials used are available from the authors or from standard commercial sources (Sigma-Aldrich, Lonza, Gibco Thermofisher) Antibodies Antibodies used mouse Cytokeratin14 antibody (KRT14/532) [DyLight 488] from Novus Biologicals Validation Novusbio guarantees primary and secondary antibodies of homology >90% within one year from receipt. .. Authors validated and optimized Antibody concentration with signal on dermis region Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Males, 6 week-old NCR nude mice or C57Bl6 mice were obtained from InVivos Singapore, used within 2 weeks of delivery Wild animals No wild animals were used in this study Field-collected samples This study does not involve field collection of samples

    Chromatin Immunoprecipitation:

    Article Title: Framework nucleic acids as programmable carrier for transdermal drug delivery
    Article Snippet: .. Such sample size was chosen with referral to similar studies previously Data exclusions No data were excluded from the analyses, except for outliers as determined with Tukey's fences Replication All in vitro analyses were repeated at least three times with n>=3; all in vivo analyses were repeated at least twice Randomization Animals, ex vivo tissues were allocated randomly to different treatment groups Blinding Single blinding was performed for all animal studies, where personnel in charge of data collection and analyses is not aware of treatment conditions Reporting for specific materials, systems and methods Materials & experimental systems n/a Involved in the study Unique biological materials Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Unique biological materials Policy information about availability of materials Obtaining unique materials All unique materials used are available from the authors or from standard commercial sources (Sigma-Aldrich, Lonza, Gibco Thermofisher) Antibodies Antibodies used mouse Cytokeratin14 antibody (KRT14/532) [DyLight 488] from Novus Biologicals Validation Novusbio guarantees primary and secondary antibodies of homology >90% within one year from receipt. .. Authors validated and optimized Antibody concentration with signal on dermis region Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Males, 6 week-old NCR nude mice or C57Bl6 mice were obtained from InVivos Singapore, used within 2 weeks of delivery Wild animals No wild animals were used in this study Field-collected samples This study does not involve field collection of samples

    Flow Cytometry:

    Article Title: Framework nucleic acids as programmable carrier for transdermal drug delivery
    Article Snippet: .. Such sample size was chosen with referral to similar studies previously Data exclusions No data were excluded from the analyses, except for outliers as determined with Tukey's fences Replication All in vitro analyses were repeated at least three times with n>=3; all in vivo analyses were repeated at least twice Randomization Animals, ex vivo tissues were allocated randomly to different treatment groups Blinding Single blinding was performed for all animal studies, where personnel in charge of data collection and analyses is not aware of treatment conditions Reporting for specific materials, systems and methods Materials & experimental systems n/a Involved in the study Unique biological materials Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Unique biological materials Policy information about availability of materials Obtaining unique materials All unique materials used are available from the authors or from standard commercial sources (Sigma-Aldrich, Lonza, Gibco Thermofisher) Antibodies Antibodies used mouse Cytokeratin14 antibody (KRT14/532) [DyLight 488] from Novus Biologicals Validation Novusbio guarantees primary and secondary antibodies of homology >90% within one year from receipt. .. Authors validated and optimized Antibody concentration with signal on dermis region Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Males, 6 week-old NCR nude mice or C57Bl6 mice were obtained from InVivos Singapore, used within 2 weeks of delivery Wild animals No wild animals were used in this study Field-collected samples This study does not involve field collection of samples

    Magnetic Resonance Imaging:

    Article Title: Framework nucleic acids as programmable carrier for transdermal drug delivery
    Article Snippet: .. Such sample size was chosen with referral to similar studies previously Data exclusions No data were excluded from the analyses, except for outliers as determined with Tukey's fences Replication All in vitro analyses were repeated at least three times with n>=3; all in vivo analyses were repeated at least twice Randomization Animals, ex vivo tissues were allocated randomly to different treatment groups Blinding Single blinding was performed for all animal studies, where personnel in charge of data collection and analyses is not aware of treatment conditions Reporting for specific materials, systems and methods Materials & experimental systems n/a Involved in the study Unique biological materials Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Unique biological materials Policy information about availability of materials Obtaining unique materials All unique materials used are available from the authors or from standard commercial sources (Sigma-Aldrich, Lonza, Gibco Thermofisher) Antibodies Antibodies used mouse Cytokeratin14 antibody (KRT14/532) [DyLight 488] from Novus Biologicals Validation Novusbio guarantees primary and secondary antibodies of homology >90% within one year from receipt. .. Authors validated and optimized Antibody concentration with signal on dermis region Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Males, 6 week-old NCR nude mice or C57Bl6 mice were obtained from InVivos Singapore, used within 2 weeks of delivery Wild animals No wild animals were used in this study Field-collected samples This study does not involve field collection of samples

    Biomarker Discovery:

    Article Title: Framework nucleic acids as programmable carrier for transdermal drug delivery
    Article Snippet: .. Such sample size was chosen with referral to similar studies previously Data exclusions No data were excluded from the analyses, except for outliers as determined with Tukey's fences Replication All in vitro analyses were repeated at least three times with n>=3; all in vivo analyses were repeated at least twice Randomization Animals, ex vivo tissues were allocated randomly to different treatment groups Blinding Single blinding was performed for all animal studies, where personnel in charge of data collection and analyses is not aware of treatment conditions Reporting for specific materials, systems and methods Materials & experimental systems n/a Involved in the study Unique biological materials Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Unique biological materials Policy information about availability of materials Obtaining unique materials All unique materials used are available from the authors or from standard commercial sources (Sigma-Aldrich, Lonza, Gibco Thermofisher) Antibodies Antibodies used mouse Cytokeratin14 antibody (KRT14/532) [DyLight 488] from Novus Biologicals Validation Novusbio guarantees primary and secondary antibodies of homology >90% within one year from receipt. .. Authors validated and optimized Antibody concentration with signal on dermis region Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Males, 6 week-old NCR nude mice or C57Bl6 mice were obtained from InVivos Singapore, used within 2 weeks of delivery Wild animals No wild animals were used in this study Field-collected samples This study does not involve field collection of samples

    other:

    Article Title: Simultaneous generation of transplantable RGC-like and corneal progenitor cells from hiPSCs using a dual-lineage platform.
    Article Snippet: AR TIC LE IN PR ES S AR TIC LE IN PR ES S AR TIC LE IN PR ES S AR TIC LE IN PR ES S AR TIC LE IN PR ES S AR TIC LE IN PR ES S Antibodies Dilution Distributor (Cat. No) Primary antibodies: PAX6 1:100 Biolegend (901301) ITGB4 1:100 Sigma (HPA036348) TP63 1:100 Abcam (ab735) Ki67 1:100 Abcam (ab15580) KRT12 1:200 Abcam (ab185627) KRT14 1:100 Novus (NBP2-45032) Recoverin 1:200 MERCK(ab5585) Tuj-1 1:200 Abcam (ab78078) Synaptophysin 1:200 Abcam (ab32127) APC-CD184 1:50 BD (555976) PE-CD117 1:100 Life technologies (A18361) AR TIC LE IN PR ES S APC-CD104 1:100 Invitrogen (MA523535) Secondary antibodies: Donkey anti-mouse (488) 1:500 Thermo Fisher (A21202) Donkey anti-rabbit (555) 1:500 Thermo Fisher (A31572) Donkey anti-mouse (555) 1:500 Thermo Fisher (A31570) Donkey anti-rabbit (488) 1:500 Thermo Fisher (A21206) Donkey anti-sheep (555) 1:500 Thermo Fisher (A21436) Genes Forward primer Reverse Primer PAX6 AGTGAATCAGCTCGGTGGTGTCTT TGCAGAATTCGGGAAATGTCGCAC Brn3a GGGCAAGAGCCATCCTTTCAA CTGTTCATCGTGTGGTACGTG Brn3b CAAGCAGCGACGCATCAAG GGGTTTGAGCGCGATCATATT ISL1 ATGACAAAACTAATATCCAGGGG CTGAAAAATTGACCAGTTGCTG RBPMSa AGTTCATTGCCAGAGAGCCA AGCGGGATAGGTGAAAGCAG RBPMSb ACCTCCTCCTGCTTTCACCT GCTGGCACTATCAGGAGACG RBPMSc CCTCTGTACCCAGCGGAGTT TGTGTTGGGCTTTGCCTCAG NEFL ATGAGTTCCTTCAGCTACGAGC CTGGGCATCAACGATCCAGA NAV1.6 CTGGAGAATGGAGGCACACAC ACGCTGCTGCTTCTCCTTGTC BCAM ATGCTGTCGCTCAGTTCTATC GGCTCTATTTCCGCTGTCTT EpCAM GCAGTTGTTGCTGGAATTGT GCATCTCACCCATCTCCTTTAT GJA-1 CTGGGTCCTGCAGATCATATTT GGCAACCTTGAGTTCTTCCT




    Similar Products

    99
    NSJ Bioreagents cytokeratin 14 antibody / ck14 / krt14
    Cytokeratin 14 Antibody / Ck14 / Krt14, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/krt14/Cytokeratin+14+Antibody+%2F+CK14+%2F+KRT14/custom%40v2175btn%4042055231
    Average 99 stars, based on 1 article reviews
    cytokeratin 14 antibody / ck14 / krt14 - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    93
    ABclonal Biotechnology krt14
    Krt14, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/krt14/KRT14+Rabbit+pAb/pm42594498-110-13-23
    Average 93 stars, based on 1 article reviews
    krt14 - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    94
    ABclonal Biotechnology keratin 14 krt14
    Keratin 14 Krt14, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/krt14/Cytokeratin+14+Rabbit+mAb/10__3892_slash_br__2026__2180-75-21-29
    Average 94 stars, based on 1 article reviews
    keratin 14 krt14 - by Bioz Stars, 2026-11
    94/100 stars
      Buy from Supplier

    86
    Technology Development Co krt14 creert2
    Krt14 Creert2, supplied by Technology Development Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/krt14/krt5creert2/pm42104015-52-0-12
    Average 86 stars, based on 1 article reviews
    krt14 creert2 - by Bioz Stars, 2026-11
    86/100 stars
      Buy from Supplier

    88
    Thermo Fisher gene exp krt14 mm00516876 m1
    The 3D culture of esophageal organoids in EEM hydrogel. a) Brightfield images of esophageal organoids cultured in various concentrations of EEM hydrogels and MAT at day 9. b) Quantification of organoid formation efficiency in EEM hydrogels, relative to MAT ( n = 3, one-way ANOVA with Tukey's multiple comparisons test; ∗∗ p < 0.01; ns, non-significant difference with p > 0.05). c) qRT-PCR analysis to compare gene expression of a basal layer marker ( <t>Krt14</t> ) and a suprabasal layer marker ( Krt13 ) in esophageal organoids cultured in EEM hydrogels and MAT at day 9 ( n = 4, one-way ANOVA with Tukey's multiple comparisons test; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 versus MAT; ns, non-significant difference with p > 0.05). d) Relative gene expression of basal layer markers ( Sox2 and Krt14 ) and suprabasal layer markers ( Krt13 and Krt4 ) in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT at day 9 ( n = 4; unpaired two-tailed t -test; ∗∗∗ p < 0.001 versus MAT). e) Morphology of esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 9 days. f) Immunofluorescent staining for basal layer markers (CK14, SOX2, and p63), suprabasal layer markers (CK13 and CK4), F-actin, a cell-cell adhesion marker (ECAD), and a proliferation marker (Ki67) in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 12 days. g) Quantification of CK14 and CK13 positive area in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 12 days, based on immunofluorescence-stained images ( n = 4; unpaired two-tailed t -test; ∗ p < 0.05 versus MAT; ns, non-significant difference with p > 0.05). h) Live/Dead staining to assess the viability of organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 11 days. i) Representative brightfield images at passage 0, 2, 4, and 6 of esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT. Total culture days are given in parentheses. j) Comparison of gene expression of a basal layer marker ( Krt14 ) and a suprabasal layer marker ( Krt13 ) in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT at passage 0 and 6 ( n = 4; unpaired two-tailed t -test; ∗∗∗ p < 0.001 versus MAT at each passage).
    Gene Exp Krt14 Mm00516876 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/krt14/Gene+Exp%2E+Krt14%2C+Mm00516876_m1/pmc12809746-152-31-15
    Average 88 stars, based on 1 article reviews
    gene exp krt14 mm00516876 m1 - by Bioz Stars, 2026-11
    88/100 stars
      Buy from Supplier

    86
    Jackson Laboratory krt14 cre mice
    The 3D culture of esophageal organoids in EEM hydrogel. a) Brightfield images of esophageal organoids cultured in various concentrations of EEM hydrogels and MAT at day 9. b) Quantification of organoid formation efficiency in EEM hydrogels, relative to MAT ( n = 3, one-way ANOVA with Tukey's multiple comparisons test; ∗∗ p < 0.01; ns, non-significant difference with p > 0.05). c) qRT-PCR analysis to compare gene expression of a basal layer marker ( <t>Krt14</t> ) and a suprabasal layer marker ( Krt13 ) in esophageal organoids cultured in EEM hydrogels and MAT at day 9 ( n = 4, one-way ANOVA with Tukey's multiple comparisons test; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 versus MAT; ns, non-significant difference with p > 0.05). d) Relative gene expression of basal layer markers ( Sox2 and Krt14 ) and suprabasal layer markers ( Krt13 and Krt4 ) in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT at day 9 ( n = 4; unpaired two-tailed t -test; ∗∗∗ p < 0.001 versus MAT). e) Morphology of esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 9 days. f) Immunofluorescent staining for basal layer markers (CK14, SOX2, and p63), suprabasal layer markers (CK13 and CK4), F-actin, a cell-cell adhesion marker (ECAD), and a proliferation marker (Ki67) in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 12 days. g) Quantification of CK14 and CK13 positive area in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 12 days, based on immunofluorescence-stained images ( n = 4; unpaired two-tailed t -test; ∗ p < 0.05 versus MAT; ns, non-significant difference with p > 0.05). h) Live/Dead staining to assess the viability of organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 11 days. i) Representative brightfield images at passage 0, 2, 4, and 6 of esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT. Total culture days are given in parentheses. j) Comparison of gene expression of a basal layer marker ( Krt14 ) and a suprabasal layer marker ( Krt13 ) in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT at passage 0 and 6 ( n = 4; unpaired two-tailed t -test; ∗∗∗ p < 0.001 versus MAT at each passage).
    Krt14 Cre Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/krt14/cre+k14+mice/10__1091_slash_mbc__e26___01___0040-150-0-7
    Average 86 stars, based on 1 article reviews
    krt14 cre mice - by Bioz Stars, 2026-11
    86/100 stars
      Buy from Supplier

    94
    Proteintech anti cytokeratin 14
    The 3D culture of esophageal organoids in EEM hydrogel. a) Brightfield images of esophageal organoids cultured in various concentrations of EEM hydrogels and MAT at day 9. b) Quantification of organoid formation efficiency in EEM hydrogels, relative to MAT ( n = 3, one-way ANOVA with Tukey's multiple comparisons test; ∗∗ p < 0.01; ns, non-significant difference with p > 0.05). c) qRT-PCR analysis to compare gene expression of a basal layer marker ( <t>Krt14</t> ) and a suprabasal layer marker ( Krt13 ) in esophageal organoids cultured in EEM hydrogels and MAT at day 9 ( n = 4, one-way ANOVA with Tukey's multiple comparisons test; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 versus MAT; ns, non-significant difference with p > 0.05). d) Relative gene expression of basal layer markers ( Sox2 and Krt14 ) and suprabasal layer markers ( Krt13 and Krt4 ) in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT at day 9 ( n = 4; unpaired two-tailed t -test; ∗∗∗ p < 0.001 versus MAT). e) Morphology of esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 9 days. f) Immunofluorescent staining for basal layer markers (CK14, SOX2, and p63), suprabasal layer markers (CK13 and CK4), F-actin, a cell-cell adhesion marker (ECAD), and a proliferation marker (Ki67) in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 12 days. g) Quantification of CK14 and CK13 positive area in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 12 days, based on immunofluorescence-stained images ( n = 4; unpaired two-tailed t -test; ∗ p < 0.05 versus MAT; ns, non-significant difference with p > 0.05). h) Live/Dead staining to assess the viability of organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 11 days. i) Representative brightfield images at passage 0, 2, 4, and 6 of esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT. Total culture days are given in parentheses. j) Comparison of gene expression of a basal layer marker ( Krt14 ) and a suprabasal layer marker ( Krt13 ) in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT at passage 0 and 6 ( n = 4; unpaired two-tailed t -test; ∗∗∗ p < 0.001 versus MAT at each passage).
    Anti Cytokeratin 14, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/krt14/KRT14+Fusion+Protein/pmc12992106-208-14-20
    Average 94 stars, based on 1 article reviews
    anti cytokeratin 14 - by Bioz Stars, 2026-11
    94/100 stars
      Buy from Supplier

    Image Search Results


    The 3D culture of esophageal organoids in EEM hydrogel. a) Brightfield images of esophageal organoids cultured in various concentrations of EEM hydrogels and MAT at day 9. b) Quantification of organoid formation efficiency in EEM hydrogels, relative to MAT ( n = 3, one-way ANOVA with Tukey's multiple comparisons test; ∗∗ p < 0.01; ns, non-significant difference with p > 0.05). c) qRT-PCR analysis to compare gene expression of a basal layer marker ( Krt14 ) and a suprabasal layer marker ( Krt13 ) in esophageal organoids cultured in EEM hydrogels and MAT at day 9 ( n = 4, one-way ANOVA with Tukey's multiple comparisons test; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 versus MAT; ns, non-significant difference with p > 0.05). d) Relative gene expression of basal layer markers ( Sox2 and Krt14 ) and suprabasal layer markers ( Krt13 and Krt4 ) in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT at day 9 ( n = 4; unpaired two-tailed t -test; ∗∗∗ p < 0.001 versus MAT). e) Morphology of esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 9 days. f) Immunofluorescent staining for basal layer markers (CK14, SOX2, and p63), suprabasal layer markers (CK13 and CK4), F-actin, a cell-cell adhesion marker (ECAD), and a proliferation marker (Ki67) in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 12 days. g) Quantification of CK14 and CK13 positive area in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 12 days, based on immunofluorescence-stained images ( n = 4; unpaired two-tailed t -test; ∗ p < 0.05 versus MAT; ns, non-significant difference with p > 0.05). h) Live/Dead staining to assess the viability of organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 11 days. i) Representative brightfield images at passage 0, 2, 4, and 6 of esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT. Total culture days are given in parentheses. j) Comparison of gene expression of a basal layer marker ( Krt14 ) and a suprabasal layer marker ( Krt13 ) in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT at passage 0 and 6 ( n = 4; unpaired two-tailed t -test; ∗∗∗ p < 0.001 versus MAT at each passage).

    Journal: Bioactive Materials

    Article Title: Esophagus extracellular matrix with microenvironmental complexity for esophageal organoids

    doi: 10.1016/j.bioactmat.2025.12.046

    Figure Lengend Snippet: The 3D culture of esophageal organoids in EEM hydrogel. a) Brightfield images of esophageal organoids cultured in various concentrations of EEM hydrogels and MAT at day 9. b) Quantification of organoid formation efficiency in EEM hydrogels, relative to MAT ( n = 3, one-way ANOVA with Tukey's multiple comparisons test; ∗∗ p < 0.01; ns, non-significant difference with p > 0.05). c) qRT-PCR analysis to compare gene expression of a basal layer marker ( Krt14 ) and a suprabasal layer marker ( Krt13 ) in esophageal organoids cultured in EEM hydrogels and MAT at day 9 ( n = 4, one-way ANOVA with Tukey's multiple comparisons test; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 versus MAT; ns, non-significant difference with p > 0.05). d) Relative gene expression of basal layer markers ( Sox2 and Krt14 ) and suprabasal layer markers ( Krt13 and Krt4 ) in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT at day 9 ( n = 4; unpaired two-tailed t -test; ∗∗∗ p < 0.001 versus MAT). e) Morphology of esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 9 days. f) Immunofluorescent staining for basal layer markers (CK14, SOX2, and p63), suprabasal layer markers (CK13 and CK4), F-actin, a cell-cell adhesion marker (ECAD), and a proliferation marker (Ki67) in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 12 days. g) Quantification of CK14 and CK13 positive area in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 12 days, based on immunofluorescence-stained images ( n = 4; unpaired two-tailed t -test; ∗ p < 0.05 versus MAT; ns, non-significant difference with p > 0.05). h) Live/Dead staining to assess the viability of organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT for 11 days. i) Representative brightfield images at passage 0, 2, 4, and 6 of esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT. Total culture days are given in parentheses. j) Comparison of gene expression of a basal layer marker ( Krt14 ) and a suprabasal layer marker ( Krt13 ) in esophageal organoids cultured in EEM hydrogel (5 mg mL −1 ) and MAT at passage 0 and 6 ( n = 4; unpaired two-tailed t -test; ∗∗∗ p < 0.001 versus MAT at each passage).

    Article Snippet: The relative gene expression was evaluated using the primers for the following genes (all from Thermo Fisher Scientific): sex-determining region Y-box 2 ( Sox2 ; Mm03053810_s1), keratin 14 ( Krt14 ; Mm00516876_m1), keratin 13 ( Krt13 ; Mm00495199_m1), keratin 4 ( Krt4 ; Mm01296260_m1), keratin 18 ( Krt18 ; Mm01601704_g1), keratin 19 ( Krt19 ; Mm00492980_m1), alpha fetoprotein ( Afp ; Mm00431715_m1), keratin 5 ( Krt5 ; Mm01305291_g1), secretoglobin family 1A member 1 ( Scgb1a1 ; Mm00442046_m1), and forkhead box J1 ( Foxj1 ; Mm01267279_m1).

    Techniques: Cell Culture, Quantitative RT-PCR, Gene Expression, Marker, Two Tailed Test, Staining, Immunofluorescence, Comparison

    Tissue-specific effects of EEM on esophageal organoid culture. a) Brightfield images of esophageal organoids cultured for 11 days in various ECM hydrogels (5 mg mL −1 ) produced from different decellularized organs. b) qRT-PCR analysis to compare gene expression of a basal layer marker ( Krt14 ) and a suprabasal layer marker ( Krt13 ) in esophageal organoids cultured in various ECM hydrogels for 11 days ( n = 4; one-way ANOVA with Tukey's multiple comparisons test; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 versus EEM; ns, non-significant difference with p > 0.05). c) Representative immunofluorescence staining images showing CK14 (a basal layer marker) and CK13 (a suprabasal layer marker) expression in esophageal organoids cultured in various ECM hydrogels for 11 days. d) Quantification of CK14 + and CK13 + areas in esophageal organoids cultured in various ECM hydrogels for 11 days, based on immunofluorescence staining ( n = 4; one-way ANOVA with Tukey's multiple comparisons test; ∗ p < 0.05 and ∗∗ p < 0.01 versus EEM; ns, non-significant difference with p > 0.05).

    Journal: Bioactive Materials

    Article Title: Esophagus extracellular matrix with microenvironmental complexity for esophageal organoids

    doi: 10.1016/j.bioactmat.2025.12.046

    Figure Lengend Snippet: Tissue-specific effects of EEM on esophageal organoid culture. a) Brightfield images of esophageal organoids cultured for 11 days in various ECM hydrogels (5 mg mL −1 ) produced from different decellularized organs. b) qRT-PCR analysis to compare gene expression of a basal layer marker ( Krt14 ) and a suprabasal layer marker ( Krt13 ) in esophageal organoids cultured in various ECM hydrogels for 11 days ( n = 4; one-way ANOVA with Tukey's multiple comparisons test; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 versus EEM; ns, non-significant difference with p > 0.05). c) Representative immunofluorescence staining images showing CK14 (a basal layer marker) and CK13 (a suprabasal layer marker) expression in esophageal organoids cultured in various ECM hydrogels for 11 days. d) Quantification of CK14 + and CK13 + areas in esophageal organoids cultured in various ECM hydrogels for 11 days, based on immunofluorescence staining ( n = 4; one-way ANOVA with Tukey's multiple comparisons test; ∗ p < 0.05 and ∗∗ p < 0.01 versus EEM; ns, non-significant difference with p > 0.05).

    Article Snippet: The relative gene expression was evaluated using the primers for the following genes (all from Thermo Fisher Scientific): sex-determining region Y-box 2 ( Sox2 ; Mm03053810_s1), keratin 14 ( Krt14 ; Mm00516876_m1), keratin 13 ( Krt13 ; Mm00495199_m1), keratin 4 ( Krt4 ; Mm01296260_m1), keratin 18 ( Krt18 ; Mm01601704_g1), keratin 19 ( Krt19 ; Mm00492980_m1), alpha fetoprotein ( Afp ; Mm00431715_m1), keratin 5 ( Krt5 ; Mm01305291_g1), secretoglobin family 1A member 1 ( Scgb1a1 ; Mm00442046_m1), and forkhead box J1 ( Foxj1 ; Mm01267279_m1).

    Techniques: Cell Culture, Produced, Quantitative RT-PCR, Gene Expression, Marker, Immunofluorescence, Staining, Expressing

    Storage of EEM as the pre-gel solution and its application in organoid culture. Graphical representation for storage of EEM pre-gel solution at a) 4 °C or d) −80 °C. Brightfield images of esophageal organoids cultured in EEM hydrogels formed with pre-gel solution stored at b) 4 °C (for 1 week–1 month) or e, g) −80 °C (for 1 month–2 years), compared to freshly prepared EEM hydrogel and MAT. Relative gene expression of a basal layer marker ( Krt14 ) and suprabasal layer markers ( Krt13 and Krt4 ) in esophageal organoids cultured in EEM hydrogels formed with pre-gel solution stored at c) 4 °C (for 1 week–1 month) or f, h) −80 °C (for 1 month–2 years), compared to freshly prepared EEM hydrogel and MAT ( n = 3–4; one-way ANOVA with Tukey's multiple comparisons test; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 versus MAT; ns, non-significant difference with p > 0.05). i) Representative images from immunofluorescence staining of CK14 (a basal layer marker) and CK13 (a suprabasal layer marker) in esophageal organoids cultured in EEM hydrogels formed with pre-gel solution stored at 4 °C or −80 °C for 2 months, compared to freshly prepared EEM hydrogel and MAT. j) Percentages of CK14 + and CK13 + areas relative to the total area in esophageal organoids based on immunofluorescence results shown in (i) ( n = 4; one-way ANOVA with Tukey's multiple comparisons test; ∗∗∗ p < 0.001 versus MAT; ns, non-significant difference with p > 0.05).

    Journal: Bioactive Materials

    Article Title: Esophagus extracellular matrix with microenvironmental complexity for esophageal organoids

    doi: 10.1016/j.bioactmat.2025.12.046

    Figure Lengend Snippet: Storage of EEM as the pre-gel solution and its application in organoid culture. Graphical representation for storage of EEM pre-gel solution at a) 4 °C or d) −80 °C. Brightfield images of esophageal organoids cultured in EEM hydrogels formed with pre-gel solution stored at b) 4 °C (for 1 week–1 month) or e, g) −80 °C (for 1 month–2 years), compared to freshly prepared EEM hydrogel and MAT. Relative gene expression of a basal layer marker ( Krt14 ) and suprabasal layer markers ( Krt13 and Krt4 ) in esophageal organoids cultured in EEM hydrogels formed with pre-gel solution stored at c) 4 °C (for 1 week–1 month) or f, h) −80 °C (for 1 month–2 years), compared to freshly prepared EEM hydrogel and MAT ( n = 3–4; one-way ANOVA with Tukey's multiple comparisons test; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 versus MAT; ns, non-significant difference with p > 0.05). i) Representative images from immunofluorescence staining of CK14 (a basal layer marker) and CK13 (a suprabasal layer marker) in esophageal organoids cultured in EEM hydrogels formed with pre-gel solution stored at 4 °C or −80 °C for 2 months, compared to freshly prepared EEM hydrogel and MAT. j) Percentages of CK14 + and CK13 + areas relative to the total area in esophageal organoids based on immunofluorescence results shown in (i) ( n = 4; one-way ANOVA with Tukey's multiple comparisons test; ∗∗∗ p < 0.001 versus MAT; ns, non-significant difference with p > 0.05).

    Article Snippet: The relative gene expression was evaluated using the primers for the following genes (all from Thermo Fisher Scientific): sex-determining region Y-box 2 ( Sox2 ; Mm03053810_s1), keratin 14 ( Krt14 ; Mm00516876_m1), keratin 13 ( Krt13 ; Mm00495199_m1), keratin 4 ( Krt4 ; Mm01296260_m1), keratin 18 ( Krt18 ; Mm01601704_g1), keratin 19 ( Krt19 ; Mm00492980_m1), alpha fetoprotein ( Afp ; Mm00431715_m1), keratin 5 ( Krt5 ; Mm01305291_g1), secretoglobin family 1A member 1 ( Scgb1a1 ; Mm00442046_m1), and forkhead box J1 ( Foxj1 ; Mm01267279_m1).

    Techniques: Cell Culture, Gene Expression, Marker, Immunofluorescence, Staining