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knockout serum replacement medium  (Tocris)


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    Tocris knockout serum replacement medium
    Knockout Serum Replacement Medium, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 376 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/knockout+serum+replacement/LDN+193189/pm41942002-77-42-56
    Average 96 stars, based on 376 article reviews
    knockout serum replacement medium - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: Autism-relevant oxysterol signaling regulates GABAergic neurogenesis and interneuron subtype specification
    Article Snippet: .. Cells were induced to neuroectoderm fate by LDN-193189 (100nM, Tocris), SB-431542 (10μM, Tocris) and XAV-939 (2μM, Stratech) from day 1-10 followed by ventralisation with a combination of recombinant Sonic Hedgehog (SHH, 200 ng/ml, Peprotech) and a small molecule SHH agonist purmorphamine (PM, 1μM, Merck) from day 11-18. .. To promote terminal differentiation and cell survival, cultures were treated with BDNF (10ng/ml, Peprotech) from day 25 until harvest.

    Knock-Out:

    Article Title: Innervated organoid compositions and methods of making same
    Article Snippet: Derivation of Neural Crest (NC) from hPSC Lines At day 0, Control or mutant hiPSCs were seeded on Matrigel-coated plate (105 cells cm-2) in iPS cell medium containing 10 ng ml-1 FGF2 (PeproTech, 100-18B) and 10 μM Y-27632. .. Differentiation was then initiated by replacing iPS cell medium with KSR medium, containing knockout DMEM plus 15% KSR (Life Technologies, 10828-028), NEAA (Life Technologies, 11140-050), L-glutamine (Life Technologies, 25030-081), β-mercaptoethanol (Life Technologies, 21985-023), LDN193189 (100 nM, Stemgent) and SB431542 (10 μM, Tocris). .. The dual SMAD inhibitors and a potent GSK inhibitor were added at different time frame during the NC induction, including LDN193189 (from day 0 to day 3), SB431542 (from day 0 to day 4), 3 μM CHIR99021 (from day 2 to day 10, Tocris Bioscience, 4423).



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    Schema to obtain induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs). (A) A method for iMSC generation via lateral plate mesoderm (LPM). To induce mesodermal lineage, canine iPSCs (ciPSCs) were cultured on Matrigel with 20 % knockout serum <t>replacement</t> (KSR) medium containing transforming growth factor β (TGFβ) signal inhibitor, SB431542 (SB). After passages, the cells were cultured on tissue-culture dishes with fetal bovine serum (FBS)-MSC medium containing basic fibroblast growth factor (bFGF). We described this iMSC induction protocol as the LPM protocol. (B) A method for iMSC generation via neural crest cells (NCCs). To induce NCCs, ciPSCs were cultured with laminine-511 (LN511) and StemFit without solution C (StemFit without C) containing glycogen synthetase kinase 3β (GSK3β) inhibitor, CHIR99031 (CHIR), SB, and bFGF. After neural specification, the cells were maintained in NCC maintenance medium, which was StemFit without C containing SB, bFGF, and epidermal growth factor (EGF) on fibronectin (FN). When NCCs differentiated into iMSCs, they were cultured with StemFit for mesenchymal stem cells and LN511. We described this iMSC induction protocol as NCC + StemFit. (C) iMSC induction method via NCCs using other iMSC culture conditions. NCCs generated as described in (B) were induced to iMSCs by culturing with PRIME-XV MSC expansion XSFM on FN. We described this iMSC induction protocol as NCC + PRIME.
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    Schema to obtain induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs). (A) A method for iMSC generation via lateral plate mesoderm (LPM). To induce mesodermal lineage, canine iPSCs (ciPSCs) were cultured on Matrigel with 20 % knockout serum replacement (KSR) medium containing transforming growth factor β (TGFβ) signal inhibitor, SB431542 (SB). After passages, the cells were cultured on tissue-culture dishes with fetal bovine serum (FBS)-MSC medium containing basic fibroblast growth factor (bFGF). We described this iMSC induction protocol as the LPM protocol. (B) A method for iMSC generation via neural crest cells (NCCs). To induce NCCs, ciPSCs were cultured with laminine-511 (LN511) and StemFit without solution C (StemFit without C) containing glycogen synthetase kinase 3β (GSK3β) inhibitor, CHIR99031 (CHIR), SB, and bFGF. After neural specification, the cells were maintained in NCC maintenance medium, which was StemFit without C containing SB, bFGF, and epidermal growth factor (EGF) on fibronectin (FN). When NCCs differentiated into iMSCs, they were cultured with StemFit for mesenchymal stem cells and LN511. We described this iMSC induction protocol as NCC + StemFit. (C) iMSC induction method via NCCs using other iMSC culture conditions. NCCs generated as described in (B) were induced to iMSCs by culturing with PRIME-XV MSC expansion XSFM on FN. We described this iMSC induction protocol as NCC + PRIME.

    Journal: Regenerative Therapy

    Article Title: Generation of canine induced pluripotent stem cell-derived mesenchymal stem cells: Comparison of differentiation strategies and cell origins

    doi: 10.1016/j.reth.2025.05.008

    Figure Lengend Snippet: Schema to obtain induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs). (A) A method for iMSC generation via lateral plate mesoderm (LPM). To induce mesodermal lineage, canine iPSCs (ciPSCs) were cultured on Matrigel with 20 % knockout serum replacement (KSR) medium containing transforming growth factor β (TGFβ) signal inhibitor, SB431542 (SB). After passages, the cells were cultured on tissue-culture dishes with fetal bovine serum (FBS)-MSC medium containing basic fibroblast growth factor (bFGF). We described this iMSC induction protocol as the LPM protocol. (B) A method for iMSC generation via neural crest cells (NCCs). To induce NCCs, ciPSCs were cultured with laminine-511 (LN511) and StemFit without solution C (StemFit without C) containing glycogen synthetase kinase 3β (GSK3β) inhibitor, CHIR99031 (CHIR), SB, and bFGF. After neural specification, the cells were maintained in NCC maintenance medium, which was StemFit without C containing SB, bFGF, and epidermal growth factor (EGF) on fibronectin (FN). When NCCs differentiated into iMSCs, they were cultured with StemFit for mesenchymal stem cells and LN511. We described this iMSC induction protocol as NCC + StemFit. (C) iMSC induction method via NCCs using other iMSC culture conditions. NCCs generated as described in (B) were induced to iMSCs by culturing with PRIME-XV MSC expansion XSFM on FN. We described this iMSC induction protocol as NCC + PRIME.

    Article Snippet: After 2–5 days, the medium was changed to KSR + SB medium, which consisted of Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/F-12; Nacalai Tesque, Kyoto, Japan) with 20 % knockout serum replacement (KSR; Thermo Fisher Scientific, Waltham, MA, USA), 2 mM L-glutamine (Nacalai Tesque), 100 IU/mL penicillin, 100 μg/mL streptomycin (Nacalai Tesque), 0.1 mM non-essential amino acid (Nacalai Tesque), 0.1 mM 2-mercaptoethanol (Thermo Fisher Scientific), and 10 μM transforming growth factor β (TGFβ) inhibitor (SB431542; Fujifilm Wako Pure Chemical Corporation, Osaka, Japan).

    Techniques: Derivative Assay, Cell Culture, Knock-Out, Generated