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acs  (ATCC)


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  • 94

    Structured Review

    ATCC acs
    Acs, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kit+formulation+buffer/Organoid+Growth+Kit+1B/pmc12816621-421-14-13
    Average 94 stars, based on 4 article reviews
    acs - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    other:

    Article Title: PARP inhibitor augments anti-tumor efficacy of DNMT inhibitor by inducing senescence in cholangiocarcinoma
    Article Snippet: CCA PDO(ATCC) was grown in organoid growth kit (ATCC, ACS-7101) and Matrigel.

    Formulation:

    Article Title: Pancreatic cancer organoid-screening captures personalized sensitivity and chemoresistance suppression upon cytochrome P450 3A5-targeted inhibition
    Article Snippet: .. The organoid medium formulation consisted of Gibco Advanced DMEM/F12 (cat. no. 12634028) supplemented with 10 mmol/L HEPES (cat. no. 15630080), 1X GlutaMAX (cat. no. 35050061), 1X B27 supplement (cat. no. 17504044), and 1X penicillin/streptomycin (cat. no. 15140122) (all from Thermo Fisher Scientific); 500 nmol/L A 83-01, 50 ng/mL EGF, 100 ng/mL noggin, 100 ng/mL FGF-10, 10 nmol/L gastrin, 1.25 mmol/L N-acetylcysteine, 10 mmol/L nicotinamide (all from the ATCC Organoid Growth Kit, cat. no. ACS-7101); 50% Wnt-3A (ATCC, cat. no CRL-2647) and 10% R-spondin (Trevigen, cat. no. 3710-001-01) conditioned medium; and 10 μmol/L ROCK Inhibitor Y-27632 (Sigma-Aldrich, cat. no. 688000). ..

    Article Title: Glycolytic heterogeneity drives metabolic-targeted therapy in pancreatic ductal adenocarcinoma
    Article Snippet: Cell motility was quantified by manual tracking via the Manual Tracking plugin in FIJI (ImageJ). .. Human PDAC organoids (HCM-CSHL-0092-C25, PDM-39, ATCC) were maintained in Organoid Media Formulation #3 (ATCC, ACS-7101), which was prepared according to the manufacturer’s instructions. .. In brief, the complete growth medium consisted of Advanced DMEM/F12 (Thermo Fisher Scientific, 12634028) supplemented with 10 mM HEPES (Thermo Fisher Scientific, 15630080), 2 mM L-glutamine (ATCC, 30-2214), 1× B-27 supplement (Thermo Fisher Scientific, 17504-044), and the following recombinant factors from the Organoid Growth Kit: Noggin (ATCC, ACS-7200), EGF (ATCC, ACS-7202), nicotinamide (ATCC, ACS-7214), N-acetylcysteine (ATCC, ACS-7215), FGF-10 (ATCC, ACS-7204), Gastrin (ATCC, ACS-7208), and A83-01 (ATCC, ACS-7209).

    Organoids:

    Article Title: Proteomics profiling of research models for studying pancreatic ductal adenocarcinoma
    Article Snippet: .. Name Reagents Manufacturer Cat number Final concentr ation Volume Human PDAC organoids PDM-41 ATCC HCM-CSHL-0094-C2 Lot 70014508 Splitting media Advanced DMEM F:12 Thermo Fischer Scientific 12634028 500 mL HEPES pH 7.2-7.5 Thermo Fischer Scientific 15630106 5 mL GlutaMAX Supplement Thermo Fischer Scientific 35050061 5 mL Primocin 50 mg/mL InvivoGen ant-pm-2 1 mL Bovine serum albumin (BSA) Sigma Aldrich Cas 9048- 46-8 1,67 mL Supplemente d basal media Advanced DMEM:F12 Thermo Fischer Scientific 12634028 90 mL HEPES (10 mM) Thermo Fischer Scientific 15630106 2,5 mL L-Glutamine Solution, 200 mM ATCC 30-2214 2,5 mL B27 supplement Thermo Fischer Scientific 17504001 5 mL Complete growth medium * RSPO1 Conditioned Media 10 % 10 mL **Wnt-3A conditioned Media 50 % 50 mL Supplemented basal media 37,4 % 37,4 mL ***Organoid growth kit 1B ATCC ACS-7101 Noggin ATCC ACS-7200 100ng/m L 0,2 mL EGF ATCC ACS-7202 50 ng/mL 0,1 mL Nicotinamide ATCC ACS-7214 10 mM 1 mL N-Acetyl- cysteine ATCC ACS-7215 1.25 mM 0,25 mL FGF-10 ATCC ACS-7204 100 ng/mL 1 mL Gastrin ATCC ACS-7208 10 nM 0,02 mL A 83-01 ATCC ACS-7209 500 nM 0,02 mL Freezing media Dimethyl sulfoxide (DMSO) Calbiochem 317275 5% 20 mL Fetal calf serum (FCS) 95% 1 mL * See Table S5 Materials and reagents for production of Wnt-3a conditioned media ** See Table S6 Materials and reagents for production of Rspondin1 conditioned media ***The organoid growth factors are reconstituted in supplemented basal media. ..



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