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resuspension buffer  (Qiagen)


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    Structured Review

    Qiagen resuspension buffer
    Resuspension Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kit+buffer/miRCURY+Exosome+Cell%2FUrine%2FCSF+Kit/pmc13065346-170-13-15
    Average 94 stars, based on 48 article reviews
    resuspension buffer - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: Epicardial extracellular vesicles modulate gene expression following ischemia-reperfusion injury in heart-on-a-chip
    Article Snippet: .. Pelleted EVs were resuspended in an appropriate volume of resuspension buffer (Qiagen, 76743) to produce a 100x concentrated EV solution relative to the initial volume of conditioned media from which they were derived. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Cerebrospinal Fluid from Restless Legs Syndrome Patients Reduces Iron Uptake in Blood-Brain Barrier Endothelial Cells by Disrupting the Regulation of Transferrin Receptors.
    Article Snippet: .. To perform the qPCR for miRNAs, we isolated the extracellular vesicles (EVs) from the 2mL/subject of CSF by using the commercial EV isolation kit (QIAGEN, USA, 76743) the manufacturer’s protocol. ..

    Isolation:

    Article Title: Cerebrospinal Fluid from Restless Legs Syndrome Patients Reduces Iron Uptake in Blood-Brain Barrier Endothelial Cells by Disrupting the Regulation of Transferrin Receptors.
    Article Snippet: .. To perform the qPCR for miRNAs, we isolated the extracellular vesicles (EVs) from the 2mL/subject of CSF by using the commercial EV isolation kit (QIAGEN, USA, 76743) the manufacturer’s protocol. ..

    Article Title: Adipose‐Derived Extracellular Vesicles and Intercellular Crosstalk With Skeletal Muscle: Implications for Sarcopenic Obesity and Metabolic Dysregulation
    Article Snippet: EV separation in this study is performed by the miRCURY exosome isolation kit (exiqon), so there is potential confounding of results by enriching for specific EV populations that this kit selects for; however, thorough characterization of the resulting EV population via TEM, NTA, and Western blotting for classical EV markers (CD9, CD81, and HSP70) demonstrates that the miRNAs identified in this work are likely EV associated. .. Katayama et al. also used the miRCURY exosome isolation kit (exiqon) to examine circulating EV‐ and non–EV‐associated miRNA profiles in BMI‐matched diabetic and nondiabetic patients; no difference existed in total circulating miRNA profile, but six differentially expressed miRNAs were present in EVs, implying specific modulation of the EV‐associated miRNAs [ ]. ..

    Article Title: The potential of exosomes in regenerative medicine and in the diagnosis and therapies of neurodegenerative diseases and cancer
    Article Snippet: .. Several commercial kits use a polyethylene glycol precipitation technology, including the Total Exosome Isolation Kit (Invitrogen), ExoQuick-TC Exosome Precipitation Solution (System Biosciences), miRCURY Exosome Kits (QIAGEN), Exo-Prep (HansaBioMed), PureExo Exosome Isolation Kit (101Bio), ExoGAG (NasasBiotech), Exosome Precipitation Solutions (Immunostep), and the miRCURY Exosome Isolation Kit (Exiqon) ( ). ..

    Article Title: Recent Exploration of Solid Cancer Biomarkers Hidden Within Urine or Blood Exosomes That Provide Fundamental Information for Future Cancer Diagnostics
    Article Snippet: .. Candidate Cancer Markers Molecular Types Isolation Methods Analysis Methods Cancer Types References miR-3940-5p/miR-8069 miRNA ExoQuick-TC (System Biosciences, Palo Alto, CA, USA) Microarray Pancrea [76] SLC9A3R1, SPAG9, and ferritin light chain (FTL) Protein Phosphatidylserine molecularly imprinted polymers Mass spectrometry Pancrea [77] Leucine-rich alpha-2-glycoprotein 1(LRG1) Glycoprotein Ultracentrifugation Mass spectrometry Ovarian [78] CD117 Protein 100 kDa Amicon Ultra-15 centrifugal filter units (Millipore, Boston, MA, USA) Flow cytometry Ovarian [79] miR-222, miR-200c, and miR-375 miRNA miRNeasy serum/plasma kit (QIAGEN, Hilden, Germany) RT-qPCR Breast [80] Raman spectrum Exosome Surface-enhanced Ramanspectroscopy Raman spectrum MCF-7, HeLa, and H8 cell lines [81] miR-542-5p and miR-320a miRNA Exo-UrineTM EV isolation kit (System Biosciences, Palo Alto, CA, USA) RNA-seq Renal [82] SNORD99 and SNORA50C snRNA miRCURY exosome cell/urine/CSF kit (QIAGEN, Hilden, Germany) RNA-seq Renal [83] lncRNA MALAT1 lncRNA Total exosome isolation kit (Invitogen, Carlsbad, CA, USA) RT-qPCR Wilms’ tumor (a rarekidney cancer) [84] Table 2. ..

    Clinical Proteomics:

    Article Title: Cerebrospinal Fluid from Restless Legs Syndrome Patients Reduces Iron Uptake in Blood-Brain Barrier Endothelial Cells by Disrupting the Regulation of Transferrin Receptors.
    Article Snippet: .. To perform the qPCR for miRNAs, we isolated the extracellular vesicles (EVs) from the 2mL/subject of CSF by using the commercial EV isolation kit (QIAGEN, USA, 76743) the manufacturer’s protocol. ..

    Biomarker Discovery:

    Article Title: Analysis of qRT-PCR Data to Identify the Most Stable Reference Gene Using gQuant
    Article Snippet: .. A comprehensive literature review and Qiagen’s Human Urine Exosomes Focus miRCURY LNA Panel list were used to select the miRNA set used in this study for validation. ..

    Microarray:

    Article Title: Recent Exploration of Solid Cancer Biomarkers Hidden Within Urine or Blood Exosomes That Provide Fundamental Information for Future Cancer Diagnostics
    Article Snippet: .. Candidate Cancer Markers Molecular Types Isolation Methods Analysis Methods Cancer Types References miR-3940-5p/miR-8069 miRNA ExoQuick-TC (System Biosciences, Palo Alto, CA, USA) Microarray Pancrea [76] SLC9A3R1, SPAG9, and ferritin light chain (FTL) Protein Phosphatidylserine molecularly imprinted polymers Mass spectrometry Pancrea [77] Leucine-rich alpha-2-glycoprotein 1(LRG1) Glycoprotein Ultracentrifugation Mass spectrometry Ovarian [78] CD117 Protein 100 kDa Amicon Ultra-15 centrifugal filter units (Millipore, Boston, MA, USA) Flow cytometry Ovarian [79] miR-222, miR-200c, and miR-375 miRNA miRNeasy serum/plasma kit (QIAGEN, Hilden, Germany) RT-qPCR Breast [80] Raman spectrum Exosome Surface-enhanced Ramanspectroscopy Raman spectrum MCF-7, HeLa, and H8 cell lines [81] miR-542-5p and miR-320a miRNA Exo-UrineTM EV isolation kit (System Biosciences, Palo Alto, CA, USA) RNA-seq Renal [82] SNORD99 and SNORA50C snRNA miRCURY exosome cell/urine/CSF kit (QIAGEN, Hilden, Germany) RNA-seq Renal [83] lncRNA MALAT1 lncRNA Total exosome isolation kit (Invitogen, Carlsbad, CA, USA) RT-qPCR Wilms’ tumor (a rarekidney cancer) [84] Table 2. ..

    Mass Spectrometry:

    Article Title: Recent Exploration of Solid Cancer Biomarkers Hidden Within Urine or Blood Exosomes That Provide Fundamental Information for Future Cancer Diagnostics
    Article Snippet: .. Candidate Cancer Markers Molecular Types Isolation Methods Analysis Methods Cancer Types References miR-3940-5p/miR-8069 miRNA ExoQuick-TC (System Biosciences, Palo Alto, CA, USA) Microarray Pancrea [76] SLC9A3R1, SPAG9, and ferritin light chain (FTL) Protein Phosphatidylserine molecularly imprinted polymers Mass spectrometry Pancrea [77] Leucine-rich alpha-2-glycoprotein 1(LRG1) Glycoprotein Ultracentrifugation Mass spectrometry Ovarian [78] CD117 Protein 100 kDa Amicon Ultra-15 centrifugal filter units (Millipore, Boston, MA, USA) Flow cytometry Ovarian [79] miR-222, miR-200c, and miR-375 miRNA miRNeasy serum/plasma kit (QIAGEN, Hilden, Germany) RT-qPCR Breast [80] Raman spectrum Exosome Surface-enhanced Ramanspectroscopy Raman spectrum MCF-7, HeLa, and H8 cell lines [81] miR-542-5p and miR-320a miRNA Exo-UrineTM EV isolation kit (System Biosciences, Palo Alto, CA, USA) RNA-seq Renal [82] SNORD99 and SNORA50C snRNA miRCURY exosome cell/urine/CSF kit (QIAGEN, Hilden, Germany) RNA-seq Renal [83] lncRNA MALAT1 lncRNA Total exosome isolation kit (Invitogen, Carlsbad, CA, USA) RT-qPCR Wilms’ tumor (a rarekidney cancer) [84] Table 2. ..

    Flow Cytometry:

    Article Title: Recent Exploration of Solid Cancer Biomarkers Hidden Within Urine or Blood Exosomes That Provide Fundamental Information for Future Cancer Diagnostics
    Article Snippet: .. Candidate Cancer Markers Molecular Types Isolation Methods Analysis Methods Cancer Types References miR-3940-5p/miR-8069 miRNA ExoQuick-TC (System Biosciences, Palo Alto, CA, USA) Microarray Pancrea [76] SLC9A3R1, SPAG9, and ferritin light chain (FTL) Protein Phosphatidylserine molecularly imprinted polymers Mass spectrometry Pancrea [77] Leucine-rich alpha-2-glycoprotein 1(LRG1) Glycoprotein Ultracentrifugation Mass spectrometry Ovarian [78] CD117 Protein 100 kDa Amicon Ultra-15 centrifugal filter units (Millipore, Boston, MA, USA) Flow cytometry Ovarian [79] miR-222, miR-200c, and miR-375 miRNA miRNeasy serum/plasma kit (QIAGEN, Hilden, Germany) RT-qPCR Breast [80] Raman spectrum Exosome Surface-enhanced Ramanspectroscopy Raman spectrum MCF-7, HeLa, and H8 cell lines [81] miR-542-5p and miR-320a miRNA Exo-UrineTM EV isolation kit (System Biosciences, Palo Alto, CA, USA) RNA-seq Renal [82] SNORD99 and SNORA50C snRNA miRCURY exosome cell/urine/CSF kit (QIAGEN, Hilden, Germany) RNA-seq Renal [83] lncRNA MALAT1 lncRNA Total exosome isolation kit (Invitogen, Carlsbad, CA, USA) RT-qPCR Wilms’ tumor (a rarekidney cancer) [84] Table 2. ..

    Quantitative RT-PCR:

    Article Title: Recent Exploration of Solid Cancer Biomarkers Hidden Within Urine or Blood Exosomes That Provide Fundamental Information for Future Cancer Diagnostics
    Article Snippet: .. Candidate Cancer Markers Molecular Types Isolation Methods Analysis Methods Cancer Types References miR-3940-5p/miR-8069 miRNA ExoQuick-TC (System Biosciences, Palo Alto, CA, USA) Microarray Pancrea [76] SLC9A3R1, SPAG9, and ferritin light chain (FTL) Protein Phosphatidylserine molecularly imprinted polymers Mass spectrometry Pancrea [77] Leucine-rich alpha-2-glycoprotein 1(LRG1) Glycoprotein Ultracentrifugation Mass spectrometry Ovarian [78] CD117 Protein 100 kDa Amicon Ultra-15 centrifugal filter units (Millipore, Boston, MA, USA) Flow cytometry Ovarian [79] miR-222, miR-200c, and miR-375 miRNA miRNeasy serum/plasma kit (QIAGEN, Hilden, Germany) RT-qPCR Breast [80] Raman spectrum Exosome Surface-enhanced Ramanspectroscopy Raman spectrum MCF-7, HeLa, and H8 cell lines [81] miR-542-5p and miR-320a miRNA Exo-UrineTM EV isolation kit (System Biosciences, Palo Alto, CA, USA) RNA-seq Renal [82] SNORD99 and SNORA50C snRNA miRCURY exosome cell/urine/CSF kit (QIAGEN, Hilden, Germany) RNA-seq Renal [83] lncRNA MALAT1 lncRNA Total exosome isolation kit (Invitogen, Carlsbad, CA, USA) RT-qPCR Wilms’ tumor (a rarekidney cancer) [84] Table 2. ..

    RNA Sequencing:

    Article Title: Recent Exploration of Solid Cancer Biomarkers Hidden Within Urine or Blood Exosomes That Provide Fundamental Information for Future Cancer Diagnostics
    Article Snippet: .. Candidate Cancer Markers Molecular Types Isolation Methods Analysis Methods Cancer Types References miR-3940-5p/miR-8069 miRNA ExoQuick-TC (System Biosciences, Palo Alto, CA, USA) Microarray Pancrea [76] SLC9A3R1, SPAG9, and ferritin light chain (FTL) Protein Phosphatidylserine molecularly imprinted polymers Mass spectrometry Pancrea [77] Leucine-rich alpha-2-glycoprotein 1(LRG1) Glycoprotein Ultracentrifugation Mass spectrometry Ovarian [78] CD117 Protein 100 kDa Amicon Ultra-15 centrifugal filter units (Millipore, Boston, MA, USA) Flow cytometry Ovarian [79] miR-222, miR-200c, and miR-375 miRNA miRNeasy serum/plasma kit (QIAGEN, Hilden, Germany) RT-qPCR Breast [80] Raman spectrum Exosome Surface-enhanced Ramanspectroscopy Raman spectrum MCF-7, HeLa, and H8 cell lines [81] miR-542-5p and miR-320a miRNA Exo-UrineTM EV isolation kit (System Biosciences, Palo Alto, CA, USA) RNA-seq Renal [82] SNORD99 and SNORA50C snRNA miRCURY exosome cell/urine/CSF kit (QIAGEN, Hilden, Germany) RNA-seq Renal [83] lncRNA MALAT1 lncRNA Total exosome isolation kit (Invitogen, Carlsbad, CA, USA) RT-qPCR Wilms’ tumor (a rarekidney cancer) [84] Table 2. ..

    Wilms Tumor Assay:

    Article Title: Recent Exploration of Solid Cancer Biomarkers Hidden Within Urine or Blood Exosomes That Provide Fundamental Information for Future Cancer Diagnostics
    Article Snippet: .. Candidate Cancer Markers Molecular Types Isolation Methods Analysis Methods Cancer Types References miR-3940-5p/miR-8069 miRNA ExoQuick-TC (System Biosciences, Palo Alto, CA, USA) Microarray Pancrea [76] SLC9A3R1, SPAG9, and ferritin light chain (FTL) Protein Phosphatidylserine molecularly imprinted polymers Mass spectrometry Pancrea [77] Leucine-rich alpha-2-glycoprotein 1(LRG1) Glycoprotein Ultracentrifugation Mass spectrometry Ovarian [78] CD117 Protein 100 kDa Amicon Ultra-15 centrifugal filter units (Millipore, Boston, MA, USA) Flow cytometry Ovarian [79] miR-222, miR-200c, and miR-375 miRNA miRNeasy serum/plasma kit (QIAGEN, Hilden, Germany) RT-qPCR Breast [80] Raman spectrum Exosome Surface-enhanced Ramanspectroscopy Raman spectrum MCF-7, HeLa, and H8 cell lines [81] miR-542-5p and miR-320a miRNA Exo-UrineTM EV isolation kit (System Biosciences, Palo Alto, CA, USA) RNA-seq Renal [82] SNORD99 and SNORA50C snRNA miRCURY exosome cell/urine/CSF kit (QIAGEN, Hilden, Germany) RNA-seq Renal [83] lncRNA MALAT1 lncRNA Total exosome isolation kit (Invitogen, Carlsbad, CA, USA) RT-qPCR Wilms’ tumor (a rarekidney cancer) [84] Table 2. ..



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