annexin v binding buffer from annexin v fitc pi apoptosis kit elabscience e ck a211 (Elabscience Biotechnology)

Elabscience Biotechnology is a verified supplier

Elabscience Biotechnology manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Elabscience Biotechnology annexin v binding buffer from annexin v fitc pi apoptosis kit elabscience e ck a211

CRISPR/Cas9 induces DNA damage and apoptosis in PGCs. (A) Flow cytometry analysis 24 h after electroporation, quantifying the proportion of <t>Annexin</t> <t>V</t> + /PI + cells. The horizontal axis indicates PI and the vertical axis Annexin V. The upper-left quadrant (Annexin V + /PI + ) represents late apoptotic cells, and the lower-right quadrant (Annexin V + only) represents early apoptotic cells. Upper panels: results after electroporation with Cas9 + various gRNAs; lower panels: results with dCas9 + various gRNAs. (B) Bar graph of Annexin V + /PI + percentages across groups. Cas9 editing induced a highly significant increase in late apoptosis. (C) γ-H 2 AX foci (green) detected by immunofluorescence 24 h after electroporation. Foci appear as discrete nuclear puncta; nuclei are counterstained with DAPI (blue). Scale bar = 10 µm. (D) Quantification of γ-H 2 AX foci per cell. Cas9 targeting resulted in a significant increase in γ-H 2 AX foci per cell, whereas dCas9 with sgRNA did not. Statistical significance determined by one-way ANOVA (p-values are indicated in the figure).

Annexin V Binding Buffer From Annexin V Fitc Pi Apoptosis Kit Elabscience E Ck A211, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1303 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kit+buffer/Annexin+V-FITC%2FPI+Apoptosis+Kit/pmc13011053-141-16-25
Average 98 stars, based on 1303 article reviews

annexin v binding buffer from annexin v fitc pi apoptosis kit elabscience e ck a211 - by Bioz Stars, 2026-10

98/100 stars

Images

1) Product Images from "High genotoxicity of CRISPR/Cas9 versus limited efficacy of CRISPRi in chicken primordial germ cells"

Article Title: High genotoxicity of CRISPR/Cas9 versus limited efficacy of CRISPRi in chicken primordial germ cells

Journal: Poultry Science

doi: 10.1016/j.psj.2026.106722

CRISPR/Cas9 induces DNA damage and apoptosis in PGCs. (A) Flow cytometry analysis 24 h after electroporation, quantifying the proportion of Annexin V + /PI + cells. The horizontal axis indicates PI and the vertical axis Annexin V. The upper-left quadrant (Annexin V + /PI + ) represents late apoptotic cells, and the lower-right quadrant (Annexin V + only) represents early apoptotic cells. Upper panels: results after electroporation with Cas9 + various gRNAs; lower panels: results with dCas9 + various gRNAs. (B) Bar graph of Annexin V + /PI + percentages across groups. Cas9 editing induced a highly significant increase in late apoptosis. (C) γ-H 2 AX foci (green) detected by immunofluorescence 24 h after electroporation. Foci appear as discrete nuclear puncta; nuclei are counterstained with DAPI (blue). Scale bar = 10 µm. (D) Quantification of γ-H 2 AX foci per cell. Cas9 targeting resulted in a significant increase in γ-H 2 AX foci per cell, whereas dCas9 with sgRNA did not. Statistical significance determined by one-way ANOVA (p-values are indicated in the figure).

Figure Legend Snippet: CRISPR/Cas9 induces DNA damage and apoptosis in PGCs. (A) Flow cytometry analysis 24 h after electroporation, quantifying the proportion of Annexin V + /PI + cells. The horizontal axis indicates PI and the vertical axis Annexin V. The upper-left quadrant (Annexin V + /PI + ) represents late apoptotic cells, and the lower-right quadrant (Annexin V + only) represents early apoptotic cells. Upper panels: results after electroporation with Cas9 + various gRNAs; lower panels: results with dCas9 + various gRNAs. (B) Bar graph of Annexin V + /PI + percentages across groups. Cas9 editing induced a highly significant increase in late apoptosis. (C) γ-H 2 AX foci (green) detected by immunofluorescence 24 h after electroporation. Foci appear as discrete nuclear puncta; nuclei are counterstained with DAPI (blue). Scale bar = 10 µm. (D) Quantification of γ-H 2 AX foci per cell. Cas9 targeting resulted in a significant increase in γ-H 2 AX foci per cell, whereas dCas9 with sgRNA did not. Statistical significance determined by one-way ANOVA (p-values are indicated in the figure).

Techniques Used: CRISPR, Flow Cytometry, Electroporation, Immunofluorescence

$PGCs are more sensitive to DNA double-strand breaks than somatic cells. (A) Cell viability curves after 24 h treatment with increasing concentrations of etoposide (ETP; x-axis shows log 10 [ETP]). PGC viability dropped sharply even at low ETP doses, whereas CEF cells were more tolerant (viability data are mean ± SD of triplicates). (B) Apoptosis detection in PGCs treated with low-dose ETP. The proportion of Annexin V + /PI + cells (late apoptosis) was significantly elevated even at 0.03 µM ETP. (C) Western blot analysis of γ-H 2 AX protein in PGCs treated with varying ETP concentrations (left panel; β-actin as loading control). The bar graph (right) shows the ratio of γ-H 2 AX to β-actin band intensity, with a marked increase at 3 µM ETP (p-values are indicated in the figure). (D) Quantification of γ-H 2 AX foci per cell in THP-1 cells, male PGCs, and female PGCs after exposure to X-ray doses of 0, 2, 4, 6, and 8 Gy. After 48 h recovery, γ-H 2 AX foci increased significantly with higher radiation in THP-1, male PGCs, and female PGCs. Female PGC data points are red squares; male PGCs are blue squares; THP-1 are black circles. (E) Cell-cycle distribution of male vs. female PGCs after DNA damage. PGCs were irradiated (2, 4, 6 Gy), cultured 48 h, and analyzed by flow cytometry for cell-cycle phase (propidium iodide staining). Stacked bars show the percentage of cells in G 0 G 1 , S, and G 2 /M phases in untreated vs. irradiated cells. After damage, female PGCs predominantly arrested in G 2 /M (increased G 2 fraction), whereas male PGCs accumulated in S phase. Statistical significance by one-way ANOVA (p-values are indicated in the figure).$
Figure Legend Snippet: PGCs are more sensitive to DNA double-strand breaks than somatic cells. (A) Cell viability curves after 24 h treatment with increasing concentrations of etoposide (ETP; x-axis shows log 10 [ETP]). PGC viability dropped sharply even at low ETP doses, whereas CEF cells were more tolerant (viability data are mean ± SD of triplicates). (B) Apoptosis detection in PGCs treated with low-dose ETP. The proportion of Annexin V + /PI + cells (late apoptosis) was significantly elevated even at 0.03 µM ETP. (C) Western blot analysis of γ-H 2 AX protein in PGCs treated with varying ETP concentrations (left panel; β-actin as loading control). The bar graph (right) shows the ratio of γ-H 2 AX to β-actin band intensity, with a marked increase at 3 µM ETP (p-values are indicated in the figure). (D) Quantification of γ-H 2 AX foci per cell in THP-1 cells, male PGCs, and female PGCs after exposure to X-ray doses of 0, 2, 4, 6, and 8 Gy. After 48 h recovery, γ-H 2 AX foci increased significantly with higher radiation in THP-1, male PGCs, and female PGCs. Female PGC data points are red squares; male PGCs are blue squares; THP-1 are black circles. (E) Cell-cycle distribution of male vs. female PGCs after DNA damage. PGCs were irradiated (2, 4, 6 Gy), cultured 48 h, and analyzed by flow cytometry for cell-cycle phase (propidium iodide staining). Stacked bars show the percentage of cells in G 0 G 1 , S, and G 2 /M phases in untreated vs. irradiated cells. After damage, female PGCs predominantly arrested in G 2 /M (increased G 2 fraction), whereas male PGCs accumulated in S phase. Statistical significance by one-way ANOVA (p-values are indicated in the figure).

Techniques Used: Western Blot, Control, Irradiation, Cell Culture, Flow Cytometry, Staining

Staining:

Article Title: The NQO1 of Nile tilapia (Oreochromis niloticus) is involved in the oxidative stress induced by copper exposure.
Article Snippet: .. After 24 h of CuSO4 5H2O exposure, cells from each group were collected and stained using an Annexin V-FITC/PI Apoptosis Detection Kit (Elabscience, Wuhan, China) according to the manufacturer’s instructions. .. Apoptotic cells were then analyzed using an Attune NxT flow cytometer (Invitrogen, Thermo Fisher Scientific, USA).

Article Title: Exploring the potential mechanisms of chrysophanol in the treatment of nonalcoholic fatty liver disease based on network pharmacology and experimental validation
Article Snippet: After harvesting, the cells were washed two times with PBS (Elabscience). .. Apoptosis staining was subsequently performed using an Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit (Elabscience) following the manufacturer’s protocol. ..

Analysis:

Article Title: Isocyanate-Modified Melatplatin(IV) Enhances Antitumor Efficacy via TCF4/COL6A3-Mediated Extracellular Matrix Remodeling and cGAS-STING Pathway Activation in Bladder Cancer.
Article Snippet: .. The procedure as performed according to the guidelines of the Annexin VITC/PI Cell Apoptosis Kit (Elabscience, E-CK-A211), followed y flow cytometric analysis. ..

Organoids:

Article Title: Dual Compartmentalization of GSTA4 Suppresses Ferroptosis to Drive Antiandrogen Resistance in Prostate Cancer.
Article Snippet: .. Following treatment, organoids were tained with propidium iodide (PI) (Elabscience, E-CK-A211) for 5 min and visualized under a fluorescence microscope. ..

Fluorescence:

Microscopy:

Incubation:

Article Title: STOML2 Knockdown Amplifies PINK1/Parkin-Dependent Mitophagy in Colorectal Cancer.
Article Snippet: Colorectal cancer (CRC) remains a major cause of cancer death.. STOML2 can orchestrate mitophagy in pancreatic cancer cells.. Herein, this study probed whether STOML2 modulated cell mitophagy in CRC.

Flow Cytometry:

Labeling:

Article Title: Baihe Zhimu Decoction ameliorates anxious depression via PI3K / AKT / mTOR pathway‐mediated neuroprotection and inhibition of hippocampal neuronal apoptosis
Article Snippet: .. Cells were labeled with 5 μL Annexin V‐FITC and 5 μL PI (E‐CK‐A211, Elabscience) for 15 min at room temperature in the dark. ..

Apoptosis Assay:

Article Title: Suppression of microglial MEF2C by α-Synuclein is associated with neuroinflammation in a mouse model of Parkinson's disease and promotes degeneration In Vitro.
Article Snippet: .. Cell apoptosis was then assessed by using a apoptosis assay kit (E-CK-A211; Elabscience, Wuhan, China) according to manufacturer's protocol strictly. ..

Review