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nucleospin soil kit  (MACHEREY NAGEL)


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    Structured Review

    MACHEREY NAGEL nucleospin soil kit
    Nucleospin Soil Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kit+buffer/Lysis+Buffer+SL1/pm42276667-69-21-24
    Average 93 stars, based on 74 article reviews
    nucleospin soil kit - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Sterility:

    Article Title: Stage-Specific Microbiota Transitions Throughout Black Soldier Fly Ontogeny.
    Article Snippet: .. WS Ovipositor wash Ten gravid females per replicate were held above a sterile microcentrifuge tube filled with 700 μl lysis buffer SL1 (NucleoSpin Soil kit, Macherey-Nagel, Düren, Germany) one by one, and the ovipositor was dipped into the liquid and moved in circles for 1 min to wash microbes off of the ovipositor’s surface (n = 3). ..

    Lysis:

    Article Title: Stage-Specific Microbiota Transitions Throughout Black Soldier Fly Ontogeny.
    Article Snippet: .. WS Ovipositor wash Ten gravid females per replicate were held above a sterile microcentrifuge tube filled with 700 μl lysis buffer SL1 (NucleoSpin Soil kit, Macherey-Nagel, Düren, Germany) one by one, and the ovipositor was dipped into the liquid and moved in circles for 1 min to wash microbes off of the ovipositor’s surface (n = 3). ..

    Article Title: Sympatric Lepus spp. in the central Italian Alps host significantly different gut microbiotas.
    Article Snippet: .. Total DNA was extracted from each 50 mg subsample of a single faecal pellet with the NucleoSpin Soil mini kit (Macherey-Nagel, Germany) using the lysis buffer SL1 in combination with 50 μl of Buffer SX, as suggested by Praeg, Pauli, and Illmer [29]. .. Each sample was co-extracted with a 0.15 dose of ZymoBIOMICSTM Spike-in Control I (High Microbial Load; EuroClone, Milan, Italy), before the initial lysis step following Galla et al. [30], unless otherwise stated (Tab.

    Article Title: Terfezia claveryi MAT locus characterization uncovers evolutionary insights about sexual reproduction of Pezizomycetes and reveals mating type dynamics in mycorrhizal plants.
    Article Snippet: Three steel beads of 3 mm diameter were added to microcentrifuge tubes containing root, soil or FLM samples, which were then homogenized for 2 min at a frequency of 30 Hz using the TissueLyser II (QIAGEN, Germany) as dry material (soil) or pre-immersed in liquid nitrogen to facilitate plant and fungal cell disruption (roots and FLM). .. DNA from soil was extracted using the NucleoSpin Soil kit (MachereyNagel, Düren, Germany), following the manufacturer’s instructions with some modifications: 500 μl of the lysis buffer SL1 and 150 μl of the SX enhancer solution were used. ..

    Article Title: Opportunities, Limitations, and Soil Microbial Predictors of Yield Response to Bacillus atrophaeus and Mycorrhiza in Silage Maize
    Article Snippet: .. DNA extraction was performed from 500 g of fresh soil using the NucleoSpinTM Soil Kit (Macherey-Nagel, Düren, Germany) with lysis buffer SL1 and without enhancer. ..

    Article Title: Herd-level occurrence and spread of Theileria equi and its associated ticks on an Austrian horse farm.
    Article Snippet: Genomic DNA from whole blood samples was extracted using a High Pure® PCR Template Preparation kit (Roche Diagnostics, Mannheim, Germany) following the manufacturer's instructions. .. For DNA extraction from fecal samples, 25 mg/sample were transferred to a 20 ml collection tube for DNA extraction using the NucleoSpin® Soil kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany) according to the manufacturer's specifications with some modifications: lysis buffer (SL1) was used for sample preparation without lysis condition adjustment, and DNA elution was carried out using 100 μl of elution buffer. ..

    Article Title: Application of high carbon amendments stabilizes soil microbial community composition and improves microbial recovery after a late spring drought during winter wheat cultivation
    Article Snippet: For determination of pH values 5 g soil were shaken for 5 min in 25 ml 0.01 M CaCl2 solution and stored at room temperature for 3 h. Afterwards, pH values were analyzed using a Lab pH level 1 sensor (WTW GmbH & Co. KG, Germany). .. DNA extraction and quantitative real time PCR for bacteria and archaea DNA was extracted from 0.3 g frozen bulk soil using the NuceloSpin Soil Kit (Macherey-Nagel GmbH & CO KG, Germany) following the user manual (Lysis Buffer SL1 was used). ..

    DNA Extraction:

    Article Title: Opportunities, Limitations, and Soil Microbial Predictors of Yield Response to Bacillus atrophaeus and Mycorrhiza in Silage Maize
    Article Snippet: .. DNA extraction was performed from 500 g of fresh soil using the NucleoSpinTM Soil Kit (Macherey-Nagel, Düren, Germany) with lysis buffer SL1 and without enhancer. ..

    Article Title: Herd-level occurrence and spread of Theileria equi and its associated ticks on an Austrian horse farm.
    Article Snippet: Genomic DNA from whole blood samples was extracted using a High Pure® PCR Template Preparation kit (Roche Diagnostics, Mannheim, Germany) following the manufacturer's instructions. .. For DNA extraction from fecal samples, 25 mg/sample were transferred to a 20 ml collection tube for DNA extraction using the NucleoSpin® Soil kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany) according to the manufacturer's specifications with some modifications: lysis buffer (SL1) was used for sample preparation without lysis condition adjustment, and DNA elution was carried out using 100 μl of elution buffer. ..

    Article Title: Application of high carbon amendments stabilizes soil microbial community composition and improves microbial recovery after a late spring drought during winter wheat cultivation
    Article Snippet: For determination of pH values 5 g soil were shaken for 5 min in 25 ml 0.01 M CaCl2 solution and stored at room temperature for 3 h. Afterwards, pH values were analyzed using a Lab pH level 1 sensor (WTW GmbH & Co. KG, Germany). .. DNA extraction and quantitative real time PCR for bacteria and archaea DNA was extracted from 0.3 g frozen bulk soil using the NuceloSpin Soil Kit (Macherey-Nagel GmbH & CO KG, Germany) following the user manual (Lysis Buffer SL1 was used). ..

    Sample Prep:

    Article Title: Herd-level occurrence and spread of Theileria equi and its associated ticks on an Austrian horse farm.
    Article Snippet: Genomic DNA from whole blood samples was extracted using a High Pure® PCR Template Preparation kit (Roche Diagnostics, Mannheim, Germany) following the manufacturer's instructions. .. For DNA extraction from fecal samples, 25 mg/sample were transferred to a 20 ml collection tube for DNA extraction using the NucleoSpin® Soil kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany) according to the manufacturer's specifications with some modifications: lysis buffer (SL1) was used for sample preparation without lysis condition adjustment, and DNA elution was carried out using 100 μl of elution buffer. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Application of high carbon amendments stabilizes soil microbial community composition and improves microbial recovery after a late spring drought during winter wheat cultivation
    Article Snippet: For determination of pH values 5 g soil were shaken for 5 min in 25 ml 0.01 M CaCl2 solution and stored at room temperature for 3 h. Afterwards, pH values were analyzed using a Lab pH level 1 sensor (WTW GmbH & Co. KG, Germany). .. DNA extraction and quantitative real time PCR for bacteria and archaea DNA was extracted from 0.3 g frozen bulk soil using the NuceloSpin Soil Kit (Macherey-Nagel GmbH & CO KG, Germany) following the user manual (Lysis Buffer SL1 was used). ..

    Bacteria:

    Article Title: Application of high carbon amendments stabilizes soil microbial community composition and improves microbial recovery after a late spring drought during winter wheat cultivation
    Article Snippet: For determination of pH values 5 g soil were shaken for 5 min in 25 ml 0.01 M CaCl2 solution and stored at room temperature for 3 h. Afterwards, pH values were analyzed using a Lab pH level 1 sensor (WTW GmbH & Co. KG, Germany). .. DNA extraction and quantitative real time PCR for bacteria and archaea DNA was extracted from 0.3 g frozen bulk soil using the NuceloSpin Soil Kit (Macherey-Nagel GmbH & CO KG, Germany) following the user manual (Lysis Buffer SL1 was used). ..



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