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pbs buffer ph7 4 invitrogen am9624 bovine serum albumin sigma aldrich a9418 next gem single cell 3 ht gem kit v3 1 10 × genomics 1000351  (Thermo Fisher)


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    Thermo Fisher pbs buffer ph7 4 invitrogen am9624 bovine serum albumin sigma aldrich a9418 next gem single cell 3 ht gem kit v3 1 10 × genomics 1000351
    Pbs Buffer Ph7 4 Invitrogen Am9624 Bovine Serum Albumin Sigma Aldrich A9418 Next Gem Single Cell 3 Ht Gem Kit V3 1 10 × Genomics 1000351, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kit+buffer/Bovine+Serum+Albumin/pm42221817-206-127-130
    Average 99 stars, based on 1 article reviews
    pbs buffer ph7 4 invitrogen am9624 bovine serum albumin sigma aldrich a9418 next gem single cell 3 ht gem kit v3 1 10 × genomics 1000351 - by Bioz Stars, 2026-10
    99/100 stars

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    Incubation:

    Article Title: BMP-7 mRNA delivered by Fibrin–CaP scaffolds activates osteogenic programs in vivo as evidenced by transcriptomic and proteomic analyses
    Article Snippet: .. Fixed cells were then incubated for 1 h at room temperature in a blocking solution containing 1% bovine serum albumin (BSA; Thermo Fisher Scientific), 10% normal goat serum (R&D Systems), 0.3 M glycine, and 0.1% Tween-20 in DPBS to minimize nonspecific binding. .. Following blocking, cells were incubated overnight at 4 °C with a primary rabbit anti-human osteopontin antibody (1:200; Abcam).

    Article Title: Synergistic antibacterial effect of photocatalytic coatings activated by visible light and photodynamic therapy for rapid peri-implantitis treatment
    Article Snippet: Cytoskeleton/nuclei staining was conducted to observe hBMSCs' adhesion to coatings, as undertaken previously [ ]. .. Briefly, after 1 day of cells culture on samples surfaces the experiment was carried out as follow: cells were i) washed once in PBS; ii) fixed for 20 min with 4% formaldehyde solution; iii) permeabilized with 0.5% Triton X-100 in PBS (v/v) for 20 min; iv) washed twice with 0.05% Tween-20 in PBS (v/v); v) incubated in blocking buffer containing 1% bovine serum albumin (BSA) with 100 mM glycine (10:1 v/v) for 30 min at room temperature; vii) incubated with Texas RedTM-X Phalloidin (1:200 dilution; Thermo Fischer Scientific Inc) during 1 h for cytoskeleton staining; viii) washed with 0.05% Tween-20 in PBS (v/v); and iv) incubated with 5 mg/mL of 4, 6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for the nucleus staining (5 min). .. Then, the samples were analyzed using a fluorescence microscope (Zeiss AxioImager Z.1), and the number of nuclei per mm 2 was calculated using ImageJ software.

    Blocking Assay:

    Article Title: BMP-7 mRNA delivered by Fibrin–CaP scaffolds activates osteogenic programs in vivo as evidenced by transcriptomic and proteomic analyses
    Article Snippet: .. Fixed cells were then incubated for 1 h at room temperature in a blocking solution containing 1% bovine serum albumin (BSA; Thermo Fisher Scientific), 10% normal goat serum (R&D Systems), 0.3 M glycine, and 0.1% Tween-20 in DPBS to minimize nonspecific binding. .. Following blocking, cells were incubated overnight at 4 °C with a primary rabbit anti-human osteopontin antibody (1:200; Abcam).

    Article Title: Neonatal muscle-derived extracellular vesicles containing miR-542-3p rejuvenate aged skeletal muscle via a functional microneedle patch
    Article Snippet: .. After washing frozen muscle sections or C2C12 cell coverslips with PBS, the samples were fixed 4% paraformaldehyde (PFA, Beyotime Biotechnology, China) for 10 min. After three PBS washes, the samples were permeabilized with 0.1% Triton X-100 in PBS for 15 min, followed by blocking with 3% bovine serum albumin (BSA, Thermo Fisher, USA) for 30 min. ..

    Article Title: Synergistic antibacterial effect of photocatalytic coatings activated by visible light and photodynamic therapy for rapid peri-implantitis treatment
    Article Snippet: Cytoskeleton/nuclei staining was conducted to observe hBMSCs' adhesion to coatings, as undertaken previously [ ]. .. Briefly, after 1 day of cells culture on samples surfaces the experiment was carried out as follow: cells were i) washed once in PBS; ii) fixed for 20 min with 4% formaldehyde solution; iii) permeabilized with 0.5% Triton X-100 in PBS (v/v) for 20 min; iv) washed twice with 0.05% Tween-20 in PBS (v/v); v) incubated in blocking buffer containing 1% bovine serum albumin (BSA) with 100 mM glycine (10:1 v/v) for 30 min at room temperature; vii) incubated with Texas RedTM-X Phalloidin (1:200 dilution; Thermo Fischer Scientific Inc) during 1 h for cytoskeleton staining; viii) washed with 0.05% Tween-20 in PBS (v/v); and iv) incubated with 5 mg/mL of 4, 6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for the nucleus staining (5 min). .. Then, the samples were analyzed using a fluorescence microscope (Zeiss AxioImager Z.1), and the number of nuclei per mm 2 was calculated using ImageJ software.

    Binding Assay:

    Article Title: BMP-7 mRNA delivered by Fibrin–CaP scaffolds activates osteogenic programs in vivo as evidenced by transcriptomic and proteomic analyses
    Article Snippet: .. Fixed cells were then incubated for 1 h at room temperature in a blocking solution containing 1% bovine serum albumin (BSA; Thermo Fisher Scientific), 10% normal goat serum (R&D Systems), 0.3 M glycine, and 0.1% Tween-20 in DPBS to minimize nonspecific binding. .. Following blocking, cells were incubated overnight at 4 °C with a primary rabbit anti-human osteopontin antibody (1:200; Abcam).

    Modification:

    Article Title: Antioxidant and anti-inflammatory effects of the extract from rhizomes of Belamcanda chinensis (L.) DC. on human keratinocyte HaCaT cells
    Article Snippet: The cell line identity was verified by matching the supplier-provided STR profile with the HaCaT reference profile from Ubigene ( https://www.ubigene.us/ ). .. The cells were grown in Dulbecco's modified Eagle's medium (Gibco; Thermo Fisher Scientific, Inc.) with 10% heat-inactivated fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.) with 100 U/ml penicillin and 100 g/ml streptomycin. ..

    Labeling:

    Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
    Article Snippet: .. Briefly, gels were formed and left in a solution of 1 mg mL −1 of fluorescein isothiocyanate (FITC) labeled bovine serum albumin (BSA, Thermo Fisher) in PBS overnight at room temperature. .. A Leica STELLARIS 5 confocal microscope with HCX PL APO CS × 63.0/1.40 OIL objective was used to bleach a region of interest with a white light laser (WLL) for 20 s (until the region was fully bleached).

    Staining:

    Article Title: Synergistic antibacterial effect of photocatalytic coatings activated by visible light and photodynamic therapy for rapid peri-implantitis treatment
    Article Snippet: Cytoskeleton/nuclei staining was conducted to observe hBMSCs' adhesion to coatings, as undertaken previously [ ]. .. Briefly, after 1 day of cells culture on samples surfaces the experiment was carried out as follow: cells were i) washed once in PBS; ii) fixed for 20 min with 4% formaldehyde solution; iii) permeabilized with 0.5% Triton X-100 in PBS (v/v) for 20 min; iv) washed twice with 0.05% Tween-20 in PBS (v/v); v) incubated in blocking buffer containing 1% bovine serum albumin (BSA) with 100 mM glycine (10:1 v/v) for 30 min at room temperature; vii) incubated with Texas RedTM-X Phalloidin (1:200 dilution; Thermo Fischer Scientific Inc) during 1 h for cytoskeleton staining; viii) washed with 0.05% Tween-20 in PBS (v/v); and iv) incubated with 5 mg/mL of 4, 6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for the nucleus staining (5 min). .. Then, the samples were analyzed using a fluorescence microscope (Zeiss AxioImager Z.1), and the number of nuclei per mm 2 was calculated using ImageJ software.

    Generated:

    Article Title: 4D piezoceramic-integrated scaffolds with bioelectric cues for skeletal muscle regeneration
    Article Snippet: Protein content was quantified using a Lowry assay (DC protein assay kit, Bio-Rad), according to the manufacturer's instructions. .. Absorbance was measured at 750 nm using a microplate reader (Multiskan FC, Thermo Scientific), and protein concentrations were determined using a standard curve generated from known concentrations of bovine serum albumin (BSA, PAA). .. The porosity of the scaffolds was evaluated both qualitatively, using Scanning Electron Microscopy (SEM) (FEI Quanta 200, ESEM), and quantitatively, using X-ray micro-computed tomography (micro-CT).

    Centrifugation:

    Article Title: Recombinant bone morphogenetic protein-2 attenuates colorectal cancer progression by orchestrating Hippo pathway activation, Yes-associated protein inhibition, and epithelial-mesenchymal transition suppression.
    Article Snippet: The cells were homogenized in Radioimmunoprecipitation Assay lysis buffer (G-Biosciences) containing protease and phosphatase inhibitors (Sigma) and incubated on ice for 30 min with gentle agitation. .. After centrifugation at 13,200 rpm for 30 min at 4°C, the supernatants were obtained, and protein concentrations were measured using a bovine serum albumin-based quantification kit (Thermo Fisher Scientific). .. Equal amounts of protein (30 μg) were separated using SDS-PAGE and transferred to polyvinylidene fluoride membranes (Amersham).



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