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dolichos biflorus agglutinin dba  (Vector Laboratories)


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    Vector Laboratories dolichos biflorus agglutinin dba
    (A) RT-PCR (left panel) and qPCR (right panel) analysis showing Xbp1s transcript levels in mouse kidney is increased by lipopolysaccharide (LPS; CTR and LPS-injected, n=3 each), but not by other acute kidney injury (AKI) insults such as folic acid (FA; CTR and FA, n=3 each) or ischemia-reperfusion injury (IRI; CTR and IRI, n=4) compared to respective control. (B) RT-PCR (left panel) and qPCR (right panel) showing that Xbp1s transcript levels are unchanged in the kidney of genetic mouse models of chronic kidney disease, i.e. type II diabetes (CTR and ob/ob mice, n=3) and polycystic kidney disease [PKD; Ksp/Cre;Pkd1F/F (Pkd1, n=3) mice at postnatal day 10, P10; Pkhd1/Cre;Pkd2F/F (Pkd2, n=3) mice at postnatal day 21, P21] compared to respective control (n=3 for each condition). Mouse kidney with known Xbp1 splicing was used as positive control. (C) Xbp1s antibody co-staining with nephron segment-specific markers revealed that Xbp1s expression was induced in epithelia cells from all nephron segments in kidneys of mice treated with LPS compared to vehicle-treated controls. LTA: lotus tetragonolobus agglutinin; NKCC2: Na+-K+-Cl- cotransporter; NCC: Na+-Cl- cotransporter; <t>DBA:</t> dolichos <t>biflorus</t> agglutinin. Scale bar, 20 μm. Bars and error bars indicate mean and SEM. Student’s unpaired t-test of injury vs. control within each model. * indicates P<0.05, compared to respective control. CTR: control.
    Dolichos Biflorus Agglutinin Dba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 409 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kidney+expression+summary+section/Unconjugated+Dolichos+Biflorus+Agglutinin+(DBA)/pmc07286357-441-3-14
    Average 94 stars, based on 409 article reviews
    dolichos biflorus agglutinin dba - by Bioz Stars, 2026-11
    94/100 stars

    Images

    1) Product Images from "Renal tubular cell spliced X-box binding protein-1 (Xbp1s) has a unique role in sepsis-induced acute kidney injury and inflammation"

    Article Title: Renal tubular cell spliced X-box binding protein-1 (Xbp1s) has a unique role in sepsis-induced acute kidney injury and inflammation

    Journal: Kidney international

    doi: 10.1016/j.kint.2019.06.023

    (A) RT-PCR (left panel) and qPCR (right panel) analysis showing Xbp1s transcript levels in mouse kidney is increased by lipopolysaccharide (LPS; CTR and LPS-injected, n=3 each), but not by other acute kidney injury (AKI) insults such as folic acid (FA; CTR and FA, n=3 each) or ischemia-reperfusion injury (IRI; CTR and IRI, n=4) compared to respective control. (B) RT-PCR (left panel) and qPCR (right panel) showing that Xbp1s transcript levels are unchanged in the kidney of genetic mouse models of chronic kidney disease, i.e. type II diabetes (CTR and ob/ob mice, n=3) and polycystic kidney disease [PKD; Ksp/Cre;Pkd1F/F (Pkd1, n=3) mice at postnatal day 10, P10; Pkhd1/Cre;Pkd2F/F (Pkd2, n=3) mice at postnatal day 21, P21] compared to respective control (n=3 for each condition). Mouse kidney with known Xbp1 splicing was used as positive control. (C) Xbp1s antibody co-staining with nephron segment-specific markers revealed that Xbp1s expression was induced in epithelia cells from all nephron segments in kidneys of mice treated with LPS compared to vehicle-treated controls. LTA: lotus tetragonolobus agglutinin; NKCC2: Na+-K+-Cl- cotransporter; NCC: Na+-Cl- cotransporter; DBA: dolichos biflorus agglutinin. Scale bar, 20 μm. Bars and error bars indicate mean and SEM. Student’s unpaired t-test of injury vs. control within each model. * indicates P<0.05, compared to respective control. CTR: control.
    Figure Legend Snippet: (A) RT-PCR (left panel) and qPCR (right panel) analysis showing Xbp1s transcript levels in mouse kidney is increased by lipopolysaccharide (LPS; CTR and LPS-injected, n=3 each), but not by other acute kidney injury (AKI) insults such as folic acid (FA; CTR and FA, n=3 each) or ischemia-reperfusion injury (IRI; CTR and IRI, n=4) compared to respective control. (B) RT-PCR (left panel) and qPCR (right panel) showing that Xbp1s transcript levels are unchanged in the kidney of genetic mouse models of chronic kidney disease, i.e. type II diabetes (CTR and ob/ob mice, n=3) and polycystic kidney disease [PKD; Ksp/Cre;Pkd1F/F (Pkd1, n=3) mice at postnatal day 10, P10; Pkhd1/Cre;Pkd2F/F (Pkd2, n=3) mice at postnatal day 21, P21] compared to respective control (n=3 for each condition). Mouse kidney with known Xbp1 splicing was used as positive control. (C) Xbp1s antibody co-staining with nephron segment-specific markers revealed that Xbp1s expression was induced in epithelia cells from all nephron segments in kidneys of mice treated with LPS compared to vehicle-treated controls. LTA: lotus tetragonolobus agglutinin; NKCC2: Na+-K+-Cl- cotransporter; NCC: Na+-Cl- cotransporter; DBA: dolichos biflorus agglutinin. Scale bar, 20 μm. Bars and error bars indicate mean and SEM. Student’s unpaired t-test of injury vs. control within each model. * indicates P<0.05, compared to respective control. CTR: control.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Injection, Positive Control, Staining, Expressing

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    Immunofluorescence:

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    Article Snippet: The secondary antibodies included: IRDye 680RD Goat anti‐Rabbit IgG (1:5000 for WB), IRDye 800CW Goat anti‐Mouse IgG (1:5000 for WB) (LI‐COR Biosciences, USA), HRP‐labeled Goat Anti‐Rabbit IgG (1:2000 for WB), HRP‐labeled Goat Anti‐Mouse IgG (Beyotime Biotechnology, China) (1:2000 for WB), ABflo 405‐conjugated Goat Anti‐Rabbit IgG (ABclonal, China) (1:5000 for IFA), Alexa Fluor 488‐conjugated goat anti‐mouse IgG (1:2000 for IFA), Alexa Fluor 594‐conjugated goat anti‐mouse IgG (1:2000 for IFA), Alexa Fluor 488‐conjugated goat anti‐rabbit IgG (1:2000 for IFA), Alexa Fluor 594‐conjugated goat anti‐rabbit IgG (1:2000 for IFA) (Fisher Scientific, USA). .. FITC‐conjugated Dolichos biflorus agglutinin (DBA) (Vector Laboratories, Burlingame, USA) (1:500 for IFA) was used for Toxoplasma cyst staining. .. IFA images were captured using an Olympus BX53 microscope (Olympus Life Science, Japan) equipped with an AxioCam 503 mono camera (Zeiss, Germany).

    Article Title: GRA86 Is a Novel Dense Granule Protein Important for Virulence and Bradyzoite Differentiation in Toxoplasma gondii
    Article Snippet: .. Bradyzoite cysts were identified by indirect immunofluorescence using FITC-conjugated Dolichos biflorus agglutinin (DBA, Vectorlabs, Burlingame, CA, USA), which specifically binds N-acetylgalactosamine on the cyst wall [ ]. .. Parasites were co-stained with rabbit anti-IMC1 (1:500, available in our laboratory) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (1:500, Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: A Novel Nuclear Protein Complex Controlling the Expression of Developmentally Regulated Genes in Toxoplasma Gondii.
    Article Snippet: The secondary antibodies included: IRDye 680RD Goat anti-Rabbit IgG (1:5000 for WB), IRDye 800CW Goat anti-Mouse IgG (1:5000 for WB) (LI-COR Biosciences, USA), HRP-labeled Goat AntiRabbit IgG (1:2000 for WB), HRP-labeled Goat Anti-Mouse IgG (Beyotime Biotechnology, China) (1:2000 for WB), ABflo 405-conjugated Goat AntiRabbit IgG (ABclonal, China) (1:5000 for IFA), Alexa Fluor 488-conjugated goat anti-mouse IgG (1:2000 for IFA), Alexa Fluor 594-conjugated goat anti-mouse IgG (1:2000 for IFA), Alexa Fluor 488-conjugated goat antirabbit IgG (1:2000 for IFA), Alexa Fluor 594-conjugated goat anti-rabbit IgG (1:2000 for IFA) (Fisher Scientific, USA). .. FITC-conjugated Dolichos biflorus agglutinin (DBA) (Vector Laboratories, Burlingame, USA) (1:500 for IFA) was used for Toxoplasma cyst staining. .. IFA images were captured using an Olympus BX53 microscope (Olympus Life Science, Japan) equipped with an AxioCam 503 mono camera (Zeiss, Germany).

    Staining:

    Article Title: A Novel Nuclear Protein Complex Controlling the Expression of Developmentally Regulated Genes in Toxoplasma Gondii
    Article Snippet: The secondary antibodies included: IRDye 680RD Goat anti‐Rabbit IgG (1:5000 for WB), IRDye 800CW Goat anti‐Mouse IgG (1:5000 for WB) (LI‐COR Biosciences, USA), HRP‐labeled Goat Anti‐Rabbit IgG (1:2000 for WB), HRP‐labeled Goat Anti‐Mouse IgG (Beyotime Biotechnology, China) (1:2000 for WB), ABflo 405‐conjugated Goat Anti‐Rabbit IgG (ABclonal, China) (1:5000 for IFA), Alexa Fluor 488‐conjugated goat anti‐mouse IgG (1:2000 for IFA), Alexa Fluor 594‐conjugated goat anti‐mouse IgG (1:2000 for IFA), Alexa Fluor 488‐conjugated goat anti‐rabbit IgG (1:2000 for IFA), Alexa Fluor 594‐conjugated goat anti‐rabbit IgG (1:2000 for IFA) (Fisher Scientific, USA). .. FITC‐conjugated Dolichos biflorus agglutinin (DBA) (Vector Laboratories, Burlingame, USA) (1:500 for IFA) was used for Toxoplasma cyst staining. .. IFA images were captured using an Olympus BX53 microscope (Olympus Life Science, Japan) equipped with an AxioCam 503 mono camera (Zeiss, Germany).

    Article Title: Effects of exercise on vascular remodelling and fetal growth in uncomplicated and abortion-prone mouse pregnancies
    Article Snippet: .. To visualise uNK cells within GD10.5 implantation sites, tissue sections were stained using Dolichos Biflorus Agglutinin (DBA) lectin (Vector Laboratories), counterstained with Weigert’s haematoxylin . ..

    Article Title: PTPA Governs Stress-Responsive Differentiation and Metabolic Homeostasis in Toxoplasma gondii .
    Article Snippet: .. Differentiation efficiency into bradyzoites was assessed using Dolichos biflorus agglutinin (DBA) staining (Vector Laboratories, California, USA). ..

    Article Title: A Novel Nuclear Protein Complex Controlling the Expression of Developmentally Regulated Genes in Toxoplasma Gondii.
    Article Snippet: The secondary antibodies included: IRDye 680RD Goat anti-Rabbit IgG (1:5000 for WB), IRDye 800CW Goat anti-Mouse IgG (1:5000 for WB) (LI-COR Biosciences, USA), HRP-labeled Goat AntiRabbit IgG (1:2000 for WB), HRP-labeled Goat Anti-Mouse IgG (Beyotime Biotechnology, China) (1:2000 for WB), ABflo 405-conjugated Goat AntiRabbit IgG (ABclonal, China) (1:5000 for IFA), Alexa Fluor 488-conjugated goat anti-mouse IgG (1:2000 for IFA), Alexa Fluor 594-conjugated goat anti-mouse IgG (1:2000 for IFA), Alexa Fluor 488-conjugated goat antirabbit IgG (1:2000 for IFA), Alexa Fluor 594-conjugated goat anti-rabbit IgG (1:2000 for IFA) (Fisher Scientific, USA). .. FITC-conjugated Dolichos biflorus agglutinin (DBA) (Vector Laboratories, Burlingame, USA) (1:500 for IFA) was used for Toxoplasma cyst staining. .. IFA images were captured using an Olympus BX53 microscope (Olympus Life Science, Japan) equipped with an AxioCam 503 mono camera (Zeiss, Germany).

    Article Title: PTPA Governs Stress-Responsive Differentiation and Metabolic Homeostasis in Toxoplasma gondii
    Article Snippet: .. Differentiation efficiency into bradyzoites was assessed using Dolichos biflorus agglutinin (DBA) staining (Vector Laboratories, California, USA). ..



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