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human keap1 enzyme linked immunosorbent assay elisa kit  (Wuhan Fine Biotech)

 
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    Structured Review

    Wuhan Fine Biotech human keap1 enzyme linked immunosorbent assay elisa kit
    Oxidative stress response and the <t>KEAP1–NRF2</t> pathway levels are linked to CAS in COPD patients. (A) ELISA detection of KEAP1 and NRF2 levels in the serum of patients. (B) KEAP1, NRF2, NQO1, and HO-1 levels in the serum of patients determined via RT-qPCR. (C) Levels of ROS and MDA, and activities of SOD and catalase in the serum of patients determined using kits. The CAS group was used as a normalization control. Data among multiple groups were compared using one-way ANOVA, followed by Tukey’s post-hoc tests with corrections for multiple comparisons. CAS, coronary atherosclerosis; COPD, chronic obstructive pulmonary disease; ROS, reactive oxygen species; MDA, malondialdehyde; SOD, superoxide dismutase.
    Human Keap1 Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by Wuhan Fine Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/keap1/assay+enzyme+immunosorbent+linked/pmc13189924-159-9-17
    Average 86 stars, based on 1 article reviews
    human keap1 enzyme linked immunosorbent assay elisa kit - by Bioz Stars, 2026-11
    86/100 stars

    Images

    1) Product Images from "The possible mechanisms linking chronic obstructive pulmonary disease and coronary atherosclerosis based on coronary computed tomography angiography and animal experiments"

    Article Title: The possible mechanisms linking chronic obstructive pulmonary disease and coronary atherosclerosis based on coronary computed tomography angiography and animal experiments

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2026.1688832

    Oxidative stress response and the KEAP1–NRF2 pathway levels are linked to CAS in COPD patients. (A) ELISA detection of KEAP1 and NRF2 levels in the serum of patients. (B) KEAP1, NRF2, NQO1, and HO-1 levels in the serum of patients determined via RT-qPCR. (C) Levels of ROS and MDA, and activities of SOD and catalase in the serum of patients determined using kits. The CAS group was used as a normalization control. Data among multiple groups were compared using one-way ANOVA, followed by Tukey’s post-hoc tests with corrections for multiple comparisons. CAS, coronary atherosclerosis; COPD, chronic obstructive pulmonary disease; ROS, reactive oxygen species; MDA, malondialdehyde; SOD, superoxide dismutase.
    Figure Legend Snippet: Oxidative stress response and the KEAP1–NRF2 pathway levels are linked to CAS in COPD patients. (A) ELISA detection of KEAP1 and NRF2 levels in the serum of patients. (B) KEAP1, NRF2, NQO1, and HO-1 levels in the serum of patients determined via RT-qPCR. (C) Levels of ROS and MDA, and activities of SOD and catalase in the serum of patients determined using kits. The CAS group was used as a normalization control. Data among multiple groups were compared using one-way ANOVA, followed by Tukey’s post-hoc tests with corrections for multiple comparisons. CAS, coronary atherosclerosis; COPD, chronic obstructive pulmonary disease; ROS, reactive oxygen species; MDA, malondialdehyde; SOD, superoxide dismutase.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Control

    KEAP1–NRF2-mediated oxidative stress participates in the occurrence of COPD combined with CAS in mice. (A) PFT for FRC, RI, Cdyn, and MV in mice. (B) The histopathological changes of lung tissues observed using H&E staining. (C) Serum lipid levels in mice measured via ELISA. (D) KEAP1, NRF2, NQO1, and HO-1 levels in the serum of mice determined via RT-qPCR. (E) Levels of ROS and MDA, and activities of SOD and catalase in the lung tissue of mice measured using kits. (F) Representative Western blotting of KEAP1, NRF2, NQO1, and HO-1 proteins in the lung tissue of mice and the quantification data. n = 6 mice for each treatment. Data among multiple groups were compared using one-way ANOVA, followed by Tukey’s post-hoc tests for multiple comparisons. COPD, chronic obstructive pulmonary disease; CAS, coronary atherosclerosis; PFT, pulmonary function test; FRC, functional residual capacity; RI, resistance index; Cdyn, dynamic compliance; MV, minute ventilation; ROS, reactive oxygen species; MDA, malondialdehyde; SOD, superoxide dismutase.
    Figure Legend Snippet: KEAP1–NRF2-mediated oxidative stress participates in the occurrence of COPD combined with CAS in mice. (A) PFT for FRC, RI, Cdyn, and MV in mice. (B) The histopathological changes of lung tissues observed using H&E staining. (C) Serum lipid levels in mice measured via ELISA. (D) KEAP1, NRF2, NQO1, and HO-1 levels in the serum of mice determined via RT-qPCR. (E) Levels of ROS and MDA, and activities of SOD and catalase in the lung tissue of mice measured using kits. (F) Representative Western blotting of KEAP1, NRF2, NQO1, and HO-1 proteins in the lung tissue of mice and the quantification data. n = 6 mice for each treatment. Data among multiple groups were compared using one-way ANOVA, followed by Tukey’s post-hoc tests for multiple comparisons. COPD, chronic obstructive pulmonary disease; CAS, coronary atherosclerosis; PFT, pulmonary function test; FRC, functional residual capacity; RI, resistance index; Cdyn, dynamic compliance; MV, minute ventilation; ROS, reactive oxygen species; MDA, malondialdehyde; SOD, superoxide dismutase.

    Techniques Used: Staining, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot, Functional Assay

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    Enzyme-linked Immunosorbent Assay:

    Article Title: Regulation of trophoblast fusion dysfunction in preeclampsia by the NCK1/EIF3D/PPARG axis.
    Article Snippet: .. Secreted β-hCG was quantified using a Human β-HCG ELISA Kit (Wuhan Fine Biotech Co., Ltd., Wuhan, China), following the manufacturer’s instructions. .. Protein purification, co-immunoprecipitation (Co-IP), and immunoblotting Placental tissues and the indicated CTB cells were lysed using cell lysis buffer (Beyotime Biotech Co., Ltd.) supplemented with the protease inhibitor phenylmethanesulfonyl fluoride (PMSF, Beyotime Biotech Co., Ltd.).

    Article Title: Chronic simultaneous inducible nitric oxide synthase (iNOS) and peripheral cannabinoid CB 1 receptors blockade ameliorates pulmonary hypertension in monocrotaline-induced rat model
    Article Snippet: .. The plasma concentrations of heme oxygenase 1 (HO-1) and N-terminal propeptide of procollagen type III (PIIINP) were measured using an enzyme-linked immunosorbent assay (ELISA), according to the manufacturer’s protocols (Wuhan Fine Biotech Co. Cat# ER1221 and ER1041). ..

    Article Title: Melatonin alleviates mitochondrial damage by regulating SIRT3‑mediated mitochondrial dynamics in granulosa cells under PCOS‑like conditions.
    Article Snippet: .. The MT content of FF was measured using a Human MT eliSa Kit (cat. no. eH3344; Wuhan Fine Biotech co., ltd.), according to the manufacturer's instructions. ..

    Article Title: Diverse roles of SERPINE1 in regulating cellular proliferation and invasion
    Article Snippet: .. The concentration of SERPINE1 in the cell culture supernatant was measured using a human SERPINE1 ELISA Kit (cat. no. EH0538; Wuhan Fine Biotech Co., Ltd.). ..

    Article Title: Serum TNF-α dynamics under chemoradiation and prognosis in high-grade glial tumors
    Article Snippet: .. Using a commercial human ELISA kit (Wuhan Fine Biotech, Wuhan, China; EH0302) on a Multiskan FC microplate reader with an automated washer (Wellwash), we measured the level of TNF-α in serum. ..

    Article Title: Biodegradable polymeric compositions, methods of preparation and uses thereof
    Article Snippet: .. The supernatant solutions of both plasma and brain extracts were analyzed by ELISA, by a Human INS ELISA Kit utilizing the sandwich principle (Wuhan Fine Biotech Co., Ltd. Wuhan, Hubei, China; Catalogue No. EH0374). ..

    Article Title: Endothelial glycocalyx perturbation in obstructive sleep apnea is associated with repetitive hypoxemia and immunothrombotic endothelial dysfunction.
    Article Snippet: Syndecan-1 was quantified using the Human CD138 (Syndecan-1) ELISA kit (Diaclone, Besançon, France; Cat# 950.640.196). .. HS was measured using the Heparan sulfate ELISA kit (Wuhan Fine Biotech, Wuhan, China; Cat# ABIN5525972). .. HA was quantified AR TIC LE IN PR ES S using the Hyaluronan (HA) ELISA kit (Echelon Biosciences, Salt Lake City, UT, USA; Cat# K-1200), detecting HA species as small as 6.4kDa.

    Article Title: The possible mechanisms linking chronic obstructive pulmonary disease and coronary atherosclerosis based on coronary computed tomography angiography and animal experiments
    Article Snippet: .. Serum KEAP1 and Nrf2 levels were measured using a human KEAP1 enzyme-linked immunosorbent assay (ELISA) kit (EH4240, Wuhan Fine Biotech Co., Ltd., Wuhan, Hubei, China) and a human Nrf2 ELISA kit (abs551899, Absin Bioscience Inc., Shanghai, China) according to the manufacturer’s instructions. ..

    Clinical Proteomics:

    Article Title: Chronic simultaneous inducible nitric oxide synthase (iNOS) and peripheral cannabinoid CB 1 receptors blockade ameliorates pulmonary hypertension in monocrotaline-induced rat model
    Article Snippet: .. The plasma concentrations of heme oxygenase 1 (HO-1) and N-terminal propeptide of procollagen type III (PIIINP) were measured using an enzyme-linked immunosorbent assay (ELISA), according to the manufacturer’s protocols (Wuhan Fine Biotech Co. Cat# ER1221 and ER1041). ..

    Article Title: Biodegradable polymeric compositions, methods of preparation and uses thereof
    Article Snippet: .. The supernatant solutions of both plasma and brain extracts were analyzed by ELISA, by a Human INS ELISA Kit utilizing the sandwich principle (Wuhan Fine Biotech Co., Ltd. Wuhan, Hubei, China; Catalogue No. EH0374). ..

    Concentration Assay:

    Article Title: Diverse roles of SERPINE1 in regulating cellular proliferation and invasion
    Article Snippet: .. The concentration of SERPINE1 in the cell culture supernatant was measured using a human SERPINE1 ELISA Kit (cat. no. EH0538; Wuhan Fine Biotech Co., Ltd.). ..

    Cell Culture:

    Article Title: Diverse roles of SERPINE1 in regulating cellular proliferation and invasion
    Article Snippet: .. The concentration of SERPINE1 in the cell culture supernatant was measured using a human SERPINE1 ELISA Kit (cat. no. EH0538; Wuhan Fine Biotech Co., Ltd.). ..



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    Image Search Results


    A , gene expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.

    Journal: The Journal of Physiology

    Article Title: Mitochondrial dynamics and repair differences in two mouse models of Friedreich's ataxia

    doi: 10.1113/JP291356

    Figure Lengend Snippet: A , gene expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.

    Article Snippet: Primary antibodies: TFAM (Cell Signalling Technology, 8076), DRP1 (Cell Signalling Technology, 8570), FIS1 (Cell Signalling Technology, 32525), MFN2 (Abcam, ab56889), OPA1 (Cell Signalling Technology, 8047), Pink1 (Abcam, 23707), Parkin (Cell Signalling Technology, 32833), p62 (Abcam, ab56416), FUNDC1 (Cell Signalling Technology, 49240), BNIP3L (Cell Signalling Technology, 12396), LC3 (Cell Signalling Technology, 12 741), ATG7 (Cell Signalling Technology, 8558), ATG12–5 (Cell Signalling Technology, 4180), LAMP1 (Abcam, 24170), CD63 (Cell Signalling Technology, 10112), Cathepsin D (Cell Signalling, 74089), GAPDH (Abcam, ab8245), αTubulin (Millipore Sigma, T6199).

    Techniques: Gene Expression, Western Blot, Expressing

    A Western blot analysis of total p62, phospho-p62 (S349), KEAP1, NRF2, and phospho-ULK1 in HCT116 and HT29 cells after 24 hours of treatment with the mentioned compounds. GAPDH served as a loading control. B-D Densitometric quantification of phospho-p62/total p62 ratio, KEAP1, and NRF2 in HCT116 and HT29 cells. Values were normalized to GAPDH. Data are shown as mean ± SD (n=3). * p < 0.05; ** p < 0.005; *** p < 0.0005; **** p < 0.0001 versus single agents (celecoxib or NDGA alone).

    Journal: bioRxiv

    Article Title: Combined celecoxib and NDGA induces a non-canonical, autophagy-independent redox cell death via p62/KEAP1 in colorectal cancer

    doi: 10.64898/2026.09.29.753356

    Figure Lengend Snippet: A Western blot analysis of total p62, phospho-p62 (S349), KEAP1, NRF2, and phospho-ULK1 in HCT116 and HT29 cells after 24 hours of treatment with the mentioned compounds. GAPDH served as a loading control. B-D Densitometric quantification of phospho-p62/total p62 ratio, KEAP1, and NRF2 in HCT116 and HT29 cells. Values were normalized to GAPDH. Data are shown as mean ± SD (n=3). * p < 0.05; ** p < 0.005; *** p < 0.0005; **** p < 0.0001 versus single agents (celecoxib or NDGA alone).

    Article Snippet: NRF-2 (A3577), KEAP-1 (A25951), SQSTM1/p62 (A19700), Phospho-SQSTM1/p62-S349 (A20737), and ALOX5 (A2877) phosphor-ULK1 S555 (AP1495) were purchased from ABclonal (Woburn, MA, USA).

    Techniques: Western Blot, Analysis, Control