lambda phage dna 48.5 kb (Toyobo)

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Toyobo lambda phage dna 48.5 kb
Lambda Phage Dna 48.5 Kb, supplied by Toyobo, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kb+lambda+phage+dna/lambda+phage+dna/pm12387882-21-0-6
Average 90 stars, based on 1 article reviews

lambda phage dna 48.5 kb - by Bioz Stars, 2026-11

90/100 stars

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Protein Concentration:

Article Title: Preparation of two types of silver-doped fluorescent carbon dots and determination of their antibacterial properties.
Article Snippet: The preparation and performance of doped antibacterial carbon dots (CDs) have been the research hotspot.. In this paper, we report the preparation of two blue light-emitting silver-doped CDs, CD-1 and CD-2, through oneand two-step routes, respectively, by using polyethyleneimine, citric acid, and AgNO3 as raw materials.. Both CDs exhibited excellent and broad-spectrum activity against gram-positive bacteria, gram-negative bacteria, and fungi.

Plasmid Preparation:

Article Title: Method for constructing chimeric plasmid library
Article Snippet: .. The unit DNA plasmid prepared in this manner was quantified by a fluorescence plate reader for SYBR GreenII, which is a nucleic acid fluorescent dye, using a calibration curve created based on a dilution series of commercially available Lambda phage genome DNA (TOYOBO). (6) Gene Assembling 11 μl of 2×ligation buffer was added to 10 μl of mixture solution comprising 0.1 fmol or greater of the unit DNAs of SEQ ID NOs: 2 to 14 for assembling of seed plasmid 1 or the unit DNAs of SEQ ID NOs: 14 to 26 for assembling of seed plasmid 2 and pGETS302-SfiI (SEQ ID NO: 1) that is a vector for gene assembling in equimolar amounts. .. The whole mixture was kept at a constant temperature of 37° C. for 5 minutes, followed by adding 1 μl of T4 DNA ligase (Takara) and keeping the mixture at a constant temperature of 37° C. for 4 hours.

Fluorescence:

Sterility:

Article Title: Method for constructing chimeric plasmid library
Article Snippet: .. 2, 3 μl of 25 mM MgSO4, 5 μl of dNTP (2 mM each), 1 μl of KOD Plus (1 unit/μl), 48 pg of lambda phage DNA (TOYOBO), 15 pmol of primers (F primer and R primer, respectively), and sterile water, and the reaction was performed with GeneAmp PCR System 9700, Applied Biosystems) according to the following program. ..

Polymerase Chain Reaction:

other:

Article Title: Method for preparing DNA unit composition, and method for creating concatenated DNA
Article Snippet: The amplified DNA unit fragments were separated in 1% agarose gel (UltraPure Agarose, Invitrogen Ltd.) made of 1×TAE buffer (prepared by diluting “Tris-acetate-EDTA stock buffer (50× concentrated) pH8.3 (at 25° C.)” manufactured by Nacalai Tesque, Inc. 50 times with milliQ water) containing 2 mg/ml of Crystal Violet (Wako Pure Chemical Industries, Ltd.), on an electrophoresis system (i-MyRun.

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