4 kb ecori dna segment (TaKaRa)
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TaKaRa
4 kb ecori dna segment
4 Kb Ecori Dna Segment, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 16041 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kb+dna+segment/EcoR+I/pm12370121-55-1-49
Average 99 stars, based on 16041 article reviews
4 Kb Ecori Dna Segment, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 16041 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kb+dna+segment/EcoR+I/pm12370121-55-1-49
Average 99 stars, based on 16041 article reviews
4 kb ecori dna segment - by Bioz Stars,
2026-11
99/100 stars
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Expressing:Article Title: Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system Article Snippet: .. The resulting fusion fragments (GFP/BmMKLP1-Head, GFP/BmMKLP1-Stalk, GFP/BmMKLP1-Tail, and GFP/BmMKLP1-Full Length) were digested with Article Title: Molecular Characterization of Soritesidine Reveals a Novel Marine Sponge DNase I-like Toxin Article Snippet: The full-length sor coding sequence was amplified from the 5′-RACE-ready cDNA using Phusion High-Fidelity DNA Polymerase (New England Biolabs). .. PCR primers containing SalI and Cloning:Article Title: Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system Article Snippet: .. The resulting fusion fragments (GFP/BmMKLP1-Head, GFP/BmMKLP1-Stalk, GFP/BmMKLP1-Tail, and GFP/BmMKLP1-Full Length) were digested with Article Title: Molecular Characterization of Soritesidine Reveals a Novel Marine Sponge DNase I-like Toxin Article Snippet: The full-length sor coding sequence was amplified from the 5′-RACE-ready cDNA using Phusion High-Fidelity DNA Polymerase (New England Biolabs). .. PCR primers containing SalI and Plasmid Preparation:Article Title: CG14075/Mmt promotes amino acid catabolism and cooperates with AKH during starvation in Drosophila. Article Snippet: The cells expressing CG14075::HA and their culture media were processed with 2× sample buffer containing cOmplete protease inhibitor (Roche) after collection and subsequently used for Western blotting. .. The CG14075::HA-expressing plasmid was constructed as follows: after Article Title: AcWRKY75 and AcMYB108 mediated ABA-GA antagonism in the regulation of pineapple internal browning. Article Snippet: .. Full-length ORF of AcWRKY75 was fused to Article Title: LAMTOR2 Reprograms Energy Metabolism to Sustain Hepatocellular Carcinoma Cell Survival Article Snippet: Hepatocellular carcinoma (HCC) is characterized by profound metabolic reprogramming to support rapid proliferation and survival under nutrient stress.. However, the central regulatory hubs that coordinate these diverse metabolic programs remain poorly understood.. Here, we identify LAMTOR2 as a critical coordinator of energy metabolism and survival in HCC cells. Article Title: Molecular Characterization of Soritesidine Reveals a Novel Marine Sponge DNase I-like Toxin Article Snippet: The full-length sor coding sequence was amplified from the 5′-RACE-ready cDNA using Phusion High-Fidelity DNA Polymerase (New England Biolabs). .. PCR primers containing SalI and Article Title: Daam-, FMNL-, and mDia-family formins jointly regulate cortical actin dynamics in melanoma cells. Article Snippet: .. For Y2H analysis, the cDNAs of murine Rho-family GTPases, RhoA (Uniprot ID:Q9QUI0), RhoB (Uniprot ID:P62746), RhoC (Uniprot ID:Q62159), RhoD (Uniprot ID:P97348), RhoF/Rif (Uniprot ID:Q8BYP3), Rac1 (Uniprot ID:P63001) and Cdc42 (Uniprot ID:P60766-1) all lacking their CAAX motifs were amplified by PCR from a B16-F1 cDNA library [60] and inserted into the Construct:Article Title: CG14075/Mmt promotes amino acid catabolism and cooperates with AKH during starvation in Drosophila. Article Snippet: The cells expressing CG14075::HA and their culture media were processed with 2× sample buffer containing cOmplete protease inhibitor (Roche) after collection and subsequently used for Western blotting. .. The CG14075::HA-expressing plasmid was constructed as follows: after Article Title: AcWRKY75 and AcMYB108 mediated ABA-GA antagonism in the regulation of pineapple internal browning. Article Snippet: .. Full-length ORF of AcWRKY75 was fused to Hemagglutination Assay:Article Title: CG14075/Mmt promotes amino acid catabolism and cooperates with AKH during starvation in Drosophila. Article Snippet: The cells expressing CG14075::HA and their culture media were processed with 2× sample buffer containing cOmplete protease inhibitor (Roche) after collection and subsequently used for Western blotting. .. The CG14075::HA-expressing plasmid was constructed as follows: after other:Article Title: EPG5 orchestrates iron compartmentalization through dual regulation in macroferritinophagy. Article Snippet: Vector assembly was achieved through double restriction enzyme digestion (Xba I, 1093, Takara; EcoR I, 1040, Takara; Xho I, 1094, Takara; BamH I, 1010, Takara; Not I, 1166, Takara; Age I, R3552, NEB) followed by ligation with Over Expression:Article Title: Enhancing the Secretion Efficiency of EGFP in Pichia pastoris Through N-Glycosylation and SOD1-Mediated Redox Regulation. Article Snippet: .. or SOD1 overexpression studies, the SOD1 gene PAS_chr4_0786) was amplified from GS115 genomic DNA sing Amplification:Article Title: Enhancing the Secretion Efficiency of EGFP in Pichia pastoris Through N-Glycosylation and SOD1-Mediated Redox Regulation. Article Snippet: .. or SOD1 overexpression studies, the SOD1 gene PAS_chr4_0786) was amplified from GS115 genomic DNA sing Article Title: Daam-, FMNL-, and mDia-family formins jointly regulate cortical actin dynamics in melanoma cells. Article Snippet: .. For Y2H analysis, the cDNAs of murine Rho-family GTPases, RhoA (Uniprot ID:Q9QUI0), RhoB (Uniprot ID:P62746), RhoC (Uniprot ID:Q62159), RhoD (Uniprot ID:P97348), RhoF/Rif (Uniprot ID:Q8BYP3), Rac1 (Uniprot ID:P63001) and Cdc42 (Uniprot ID:P60766-1) all lacking their CAAX motifs were amplified by PCR from a B16-F1 cDNA library [60] and inserted into the Purification:Article Title: LAMTOR2 Reprograms Energy Metabolism to Sustain Hepatocellular Carcinoma Cell Survival Article Snippet: Hepatocellular carcinoma (HCC) is characterized by profound metabolic reprogramming to support rapid proliferation and survival under nutrient stress.. However, the central regulatory hubs that coordinate these diverse metabolic programs remain poorly understood.. Here, we identify LAMTOR2 as a critical coordinator of energy metabolism and survival in HCC cells. Gel Extraction:Article Title: LAMTOR2 Reprograms Energy Metabolism to Sustain Hepatocellular Carcinoma Cell Survival Article Snippet: Hepatocellular carcinoma (HCC) is characterized by profound metabolic reprogramming to support rapid proliferation and survival under nutrient stress.. However, the central regulatory hubs that coordinate these diverse metabolic programs remain poorly understood.. Here, we identify LAMTOR2 as a critical coordinator of energy metabolism and survival in HCC cells. Polymerase Chain Reaction:Article Title: Molecular Characterization of Soritesidine Reveals a Novel Marine Sponge DNase I-like Toxin Article Snippet: The full-length sor coding sequence was amplified from the 5′-RACE-ready cDNA using Phusion High-Fidelity DNA Polymerase (New England Biolabs). .. PCR primers containing SalI and Article Title: Daam-, FMNL-, and mDia-family formins jointly regulate cortical actin dynamics in melanoma cells. Article Snippet: .. For Y2H analysis, the cDNAs of murine Rho-family GTPases, RhoA (Uniprot ID:Q9QUI0), RhoB (Uniprot ID:P62746), RhoC (Uniprot ID:Q62159), RhoD (Uniprot ID:P97348), RhoF/Rif (Uniprot ID:Q8BYP3), Rac1 (Uniprot ID:P63001) and Cdc42 (Uniprot ID:P60766-1) all lacking their CAAX motifs were amplified by PCR from a B16-F1 cDNA library [60] and inserted into the Analysis:Article Title: Daam-, FMNL-, and mDia-family formins jointly regulate cortical actin dynamics in melanoma cells. Article Snippet: .. For Y2H analysis, the cDNAs of murine Rho-family GTPases, RhoA (Uniprot ID:Q9QUI0), RhoB (Uniprot ID:P62746), RhoC (Uniprot ID:Q62159), RhoD (Uniprot ID:P97348), RhoF/Rif (Uniprot ID:Q8BYP3), Rac1 (Uniprot ID:P63001) and Cdc42 (Uniprot ID:P60766-1) all lacking their CAAX motifs were amplified by PCR from a B16-F1 cDNA library [60] and inserted into the cDNA Library Assay:Article Title: Daam-, FMNL-, and mDia-family formins jointly regulate cortical actin dynamics in melanoma cells. Article Snippet: .. For Y2H analysis, the cDNAs of murine Rho-family GTPases, RhoA (Uniprot ID:Q9QUI0), RhoB (Uniprot ID:P62746), RhoC (Uniprot ID:Q62159), RhoD (Uniprot ID:P97348), RhoF/Rif (Uniprot ID:Q8BYP3), Rac1 (Uniprot ID:P63001) and Cdc42 (Uniprot ID:P60766-1) all lacking their CAAX motifs were amplified by PCR from a B16-F1 cDNA library [60] and inserted into the |